LOCUS       sequence-454871-        4363 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  L0 part - CDS (without STOP codon).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4363)
  AUTHORS   Golubova D, Salmon M, Su H, Tansley C, Kaithakottil GG, Linsmith G, 
            Schudoma C, Swarbreck D, O'Connell MA, Patron NJ
  TITLE     Biosynthesis and bioactivity of anti-inflammatory triterpenoids in 
            Calendula officinalis.
  JOURNAL   Nat Commun. 2025 Jul 28;16(1):6941. doi: 10.1038/s41467-025-62269-w.
  PUBMED    40721615
REFERENCE   2  (bases 1 to 4363)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-07-28"; volume: "16(1):6941. doi"; pages: " 
            10.1038/s41467-025-62269-w"
COMMENT     Sequence Label: pEPHS0CM0054
FEATURES             Location/Qualifiers
     source          1..4363
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2407..2423)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2407..2423)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2420..2442)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(2595..3183)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2675..2694)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3316..3410)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(3431..4090)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     4005..4024
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(4091..4193)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     4280..4302
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4294..4311
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4295..4311
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 taggtctcta atgtggaagc tgaagattgg tgaaaagaat ggaaatttta acattggtga
       61 cggaactaat ggggatgaat atttgtatag cacaaacaat ttcgtgggta gacaaacatg
      121 ggagttcgac ccggatgcag gcacccagga ggagagggac caagttgaga ggcttcgaga
      181 acagttcttg atcaacaaga agaagctcgg catttcttgt tgtgcggacc tactcatgag
      241 aaatcagctg attaaggaaa gcgggatcaa tcttacgagc gagcctcctg ttcagcttgc
      301 agacgatgag gatgtaaact gtgaagctgt aacaatagca gtgagaaaag cagtccgact
      361 taatcgtgct atccaggctt gggatggcca ctggccggct gaaaatgctg gtcctctctt
      421 cttcactcct cctctgatta ttgccctcta cataagtggt actatggatg cggttctaac
      481 ggaagagcac aagaaggaga tgattcgtta catgtacatc caccagaatg aagatggagg
      541 atggggattc tatatatctg ggagaagcac aatgatagga agtgccttga attatgtggc
      601 tctaagactt cttggagaag aagctccacc ggacgacgaa tgcgctcttg ctaaagggcg
      661 taaatggata tttgatcacg gtggcgccat ctctatccct tcatggggca aggtttatct
      721 ctcggtgctt ggagtatatg aatgggaagg ttgcaaccct cttcctccag aattctggct
      781 ttttccttcc tttttacctt atcatccagc aaaaatgtgg tgttactgca gaactacata
      841 catgccaatg tcatacttgt atggtaccgg cttccatggt ccgattactg acattgttgt
      901 gtctttgagg aaagagattc ataccattcc ttaccaccaa attgattgga acaaacaaag
      961 acataattgt tgcaaggagg atttgtacta ccctcactca tacattcaag atctcttatg
     1021 ggattctctg catcattttt ccgaaccact tatcacaaaa tggcccttta agaagctaag
     1081 agagaagggt ctgaaacggg tcgttgagct aatgcgttat ggatctgagg aaggaaggta
     1141 cataaacatg ggttgtgtcg acaaggcatt gcaaatgatg tgtttttatg ccatggatcc
     1201 cgatggagtt gactggaagc gacaccttgc cagggtaccc gattacttgt ggttagctga
     1261 agatggtatg aagatgcaaa gttttggtag tcagttatgg gattgcacac ttgtaactca
     1321 ggcaattatc gctagtaata tggttgacga gtatggggat agcctaacca aagctcattt
     1381 ttacatcaaa gagtcacaga tcaaggagaa tccgaagggg gatttcatta agatgtgtcg
     1441 ccaatttaca aagggggcat ggaccttctc tgatcaagat catggttggg tcgtctcaga
     1501 ttgcactgga gaatctttga agtgtttatt ggtgctgtcg cagatgccac cagaaatttc
     1561 cggtgaaaag gctgaagtgg agagattata tgatgccgtt aatgtccttc tctacctaca
     1621 gagtccagat agtggcggtt ttgctatttg ggagccacca gttcccaaac cttatttaga
     1681 gatgttgaat ccttctgaac tttttgcaga cattgtggtt gagagagagc atgttgaatg
     1741 tactggctct ataattcagg ccttgaatac attcaagcat ctgcatcccg ggcatcggga
     1801 aaaagaaata gaagttgcta ttgagaaagg cattcagttt ttggaaaaca ggcaacaaga
     1861 gaacggttcc tggtatggtt actggggaat atgttatctc tatggcacgt ttttcgttct
     1921 gcaaggactt gtatcttgcg ggaaaaccta tgaaaattcc gaaactgttc ggaaaggtgt
     1981 caactttttg ctttcaaccc aaaattcgga aggtggttgg ggagagaact tcgagtcatg
     2041 cccacaagag aaatatattc cattggaggg gaaccgcact aattttgtgc aaacttcatg
     2101 ggctatgctt ggtcttttat gtgctggaca ggctgaaagg gatccaacac cattacacaa
     2161 ggcagcaaag ttgctcatca atggacaaat ggatgatgga gattttcctc agcaggaaat
     2221 aacgggagtg tacatgaaaa attgcatgtt acattatgca gagtatagga acttgtttcc
     2281 tctctgggcg ctgggagagt accgcaaacg tgtttggttg gcaaaacaag aaatgggttc
     2341 gagagaccta aggctaggtg gaggctcagt gatgataagt ctgcgatggt ggatgcatgt
     2401 gtcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcagagggc acaatcctat
     2461 tccgcgctat ccgacaatct ccaagacatt aggtggagtt cagttcggcg tatggcatat
     2521 gtcgctggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     2581 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     2641 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     2701 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     2761 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     2821 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     2881 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     2941 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     3001 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     3061 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     3121 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     3181 caagaagatc ctttgatctt ttctacgggg tctgacgctc tattcaacaa agccgccgtc
     3241 ccgtcaagtc agcgtaaatg ggtagggggc ttcaaatcgt ccccccatac gatataagtt
     3301 gttactagtg cttggattct caccaataaa aaacgcccgg cggcaaccga gcgttctgaa
     3361 caaatccaga tggagttctg aggtcattac tggatctatc aacaggagtc caagcgagct
     3421 cgatatcaaa ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca
     3481 ttctgccgac atggaagcca tcacaaacgg catgatgaac ctgaatcgcc agcggcatca
     3541 gcaccttgtc gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt
     3601 ccatattggc cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgagacga
     3661 aaaacatatt ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca
     3721 catcttgcga atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg
     3781 atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata
     3841 tcaccagctc accgtctttc attgccatac gaaattccgg atgagcattc atcaggcggg
     3901 caagaatgtg aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa
     3961 aggccgtaat atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg
     4021 cctcaaaatg ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt
     4081 ttttctccat tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg
     4141 gtagtgatct tatttcatta tggtgaaagt tggaacctct tacgtgccga tcaacgtctc
     4201 attgatacct gaaacaaaac ccatcgtacg gccaaggaag tctccaataa ctgtgatcca
     4261 ccacaagcgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tcatgcataa
     4321 tccgcacgca tctggaataa ggaagtgcca ttccgcctga cct
//
