LOCUS       sequence-454873-        4312 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  L0 part - CDS (without STOP codon).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4312)
  AUTHORS   Golubova D, Salmon M, Su H, Tansley C, Kaithakottil GG, Linsmith G, 
            Schudoma C, Swarbreck D, O'Connell MA, Patron NJ
  TITLE     Biosynthesis and bioactivity of anti-inflammatory triterpenoids in 
            Calendula officinalis.
  JOURNAL   Nat Commun. 2025 Jul 28;16(1):6941. doi: 10.1038/s41467-025-62269-w.
  PUBMED    40721615
REFERENCE   2  (bases 1 to 4312)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-07-28"; volume: "16(1):6941. doi"; pages: " 
            10.1038/s41467-025-62269-w"
COMMENT     Sequence Label: pEPMS0CM0026
FEATURES             Location/Qualifiers
     source          1..4312
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2356..2372)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2356..2372)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2369..2391)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(2544..3132)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2624..2643)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3265..3359)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(3380..4039)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3954..3973
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(4040..4142)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     4229..4251
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4243..4260
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4244..4260
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 taggtctcta atgtggaagt tgaagatagc agaagggaat gatccttact tatttagtac
       61 caacaatttc gtcggtaggc aaatttggga atttgatccc gacgctggta ctcccgaaga
      121 aaaacaagaa gttgaaaatg ctcgtcaaca ttatagaaac aatcgaaagg aagatgttca
      181 tccatgcagt gatttgctta tgcggatgca gttgatcaaa gagaatggca tagatctact
      241 gagtattcca ccagcaaggt taggagataa tgagcaagta aattatgaag cagtgacgag
      301 atcagtgacg aaagcagttc gattgaaccg tgcgattcaa gcaaaggatg gtcactggcc
      361 tgcagaaaac gctggcccta tgtttttcac tcctcccctc cttattgcta tgtacatcag
      421 tggagccatc gccacacatt taaccaaaga acacaagacc gaaatgatac gttatatcta
      481 caaccatcaa aacgatgatg gagggtgggg attttatatc gagggacaca gtaccatgat
      541 cgggtctgct ttgagctatg tagccctaag gttactagga gagggacctc atgatggaaa
      601 tggtgcggtt gaccgagcca gaaagtggat aattgaccat ggtggtgcaa cctcaattcc
      661 ctcatggggc aaaacttatc tatcggttct tggagtatat gaatgggaag gatgcaatcc
      721 acttccacca gaattttgga ttttccctga aactttacca tatcatccgg cgaaaatgtg
      781 gtgctattgc cggacaacat acatgcctat gtcatactta tatgggacaa aatttcatgg
      841 tccgatcact gatcttgttc tacaacttag acaagaaatt catgcgatcc cgtacaatga
      901 gataaattgg aataaacaac gacataactg ttgtaaggaa gatttgtatt accctcattc
      961 aacgttgcaa gatctattgt gggatggact tcattactta agtgaaccgt ttcttaaata
     1021 ttggcctttt aaaaagctaa gagaaagagg actcaaaaga gcagttgaat taatgcgata
     1081 tggtgctcaa caaagcagat atatgactat tggatgtgtt gaaaagagct tgcaaatgat
     1141 gtgttggtgg gcagagaacc caaatgggga tgaattcaag caccatcttg ctagagtgcc
     1201 tgattatttg tggttagcag aagatggaat gaagatgcaa agttttggga gccaattatg
     1261 ggattgcaca cttgcaacac aagcaataat agctactgat atggttgaag aatatggtga
     1321 ttcacttaaa aaagctcatt tttatatcaa agaatctcaa gttaaacaaa atccttcggg
     1381 cgattttagt caaatgtgca gacagtttac tatagggtcg tggacttttt ctgaccaaga
     1441 tcatggttgg gttgtctcag attgcacagc tgaagcatta aagtgtcttt tgttactatc
     1501 ccaaatgcca gaggaaattg tgggagaaaa agctgataat gagcgattgt atgaagctgt
     1561 taatgttctt ctttacttac agagtcctat tactggaggt tttgctattt gggagccacc
     1621 tgtcccgcaa ccatatttac aaatgttgaa tccttcagaa cttttcgcag atattgttgt
     1681 tgagaaagag catgtcgagt gtacagcatc aattattcaa gcacttttag ccttcaaacg
     1741 actgcaccca gggcacaggg agaaagaaat tgaaatttcc gtggcaaaag cagttacatt
     1801 tttggaggga aaacaacagc atgatggttc atggtatggt tattggggaa tatgctttct
     1861 atatggcaca ttctttgctt taggaggctt agtttctgct ggaaaaacat ataataacag
     1921 tgaagaaatt cgtaaagcag ttaatttttt cattttgact caaaatgaag aaggcggttg
     1981 gggagaaagt atcaaatctt gccctactga agtatacaca cccttggatg gaaaccgaac
     2041 aaacctagtt caaacatcat gggctatgct tgctcttatg ttaggtggac aggctgaaag
     2101 agatccaaca cctttgcata aagcagcaaa gatattaatt aatgcacaga tggataatgg
     2161 agattttcct caacaagaga ttactggagt ttacatgaag aattgcatgc tacattatcc
     2221 agaatacaga aacatcttcc cactttgggc acttggcgaa tatcgcaaac gagtttgggt
     2281 caatggttcg agagacctaa ggctaggtgg aggctcagtg atgataagtc tgcgatggtg
     2341 gatgcatgtg tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcagagggca
     2401 caatcctatt ccgcgctatc cgacaatctc caagacatta ggtggagttc agttcggcgt
     2461 atggcatatg tcgctggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     2521 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     2581 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     2641 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     2701 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     2761 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     2821 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     2881 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     2941 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     3001 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     3061 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     3121 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctct attcaacaaa
     3181 gccgccgtcc cgtcaagtca gcgtaaatgg gtagggggct tcaaatcgtc cccccatacg
     3241 atataagttg ttactagtgc ttggattctc accaataaaa aacgcccggc ggcaaccgag
     3301 cgttctgaac aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc
     3361 aagcgagctc gatatcaaat tacgccccgc cctgccactc atcgcagtac tgttgtaatt
     3421 cattaagcat tctgccgaca tggaagccat cacaaacggc atgatgaacc tgaatcgcca
     3481 gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga
     3541 agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg
     3601 ctgagacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt
     3661 aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac
     3721 tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac
     3781 tatcccatat caccagctca ccgtctttca ttgccatacg aaattccgga tgagcattca
     3841 tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg
     3901 tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg
     3961 actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc
     4021 cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat aactcaaaaa
     4081 atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgccgat
     4141 caacgtctca ttgatacctg aaacaaaacc catcgtacgg ccaaggaagt ctccaataac
     4201 tgtgatccac cacaagcgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt
     4261 catgcataat ccgcacgcat ctggaataag gaagtgccat tccgcctgac ct
//
