LOCUS       sequence-454876-        4366 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  L0 part - CDS (without STOP codon).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4366)
  AUTHORS   Golubova D, Salmon M, Su H, Tansley C, Kaithakottil GG, Linsmith G, 
            Schudoma C, Swarbreck D, O'Connell MA, Patron NJ
  TITLE     Biosynthesis and bioactivity of anti-inflammatory triterpenoids in 
            Calendula officinalis.
  JOURNAL   Nat Commun. 2025 Jul 28;16(1):6941. doi: 10.1038/s41467-025-62269-w.
  PUBMED    40721615
REFERENCE   2  (bases 1 to 4366)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-07-28"; volume: "16(1):6941. doi"; pages: " 
            10.1038/s41467-025-62269-w"
COMMENT     Sequence Label: pEPMS0CM0037
FEATURES             Location/Qualifiers
     source          1..4366
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2410..2426)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2410..2426)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2423..2445)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(2598..3186)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2678..2697)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3319..3413)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(3434..4093)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     4008..4027
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(4094..4196)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     4283..4305
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4297..4314
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4298..4314
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 taggtctcta atgtggaagc tcaaaatagg tgaaaagaat gggaaattca acatcggtga
       61 tggaaatggt gatgaatatt tatatagcac caataacttt gtggggagac aaacttggga
      121 gttcgaccct gatgcaggca cgcaggaaga acgtgaacaa gttgaaaggt ttcgagaaca
      181 atttttgatt aataagaaga aacttgacat cagttgttgt gcagatctgc tcatgcgaaa
      241 tcagcttatt aaggaaagcg ggatcgatct tctgagcgaa cctccagtga gacttggaga
      301 ggaggaggat gtgaactttg aagcggtaac aaccgcagtt aaaaaagcag ttcgattaaa
      361 ccgtgcaatt caagcttggg atggtcattg gccggctgaa aatgcgggtc cactcttctt
      421 cactcctccc ctgataattg ccctatacat aagtggtaca ttggatacaa tcctaacaga
      481 agaacacaag aaggagatga tccgttatat gtacatccat caaaatgaag atggaggatg
      541 gggattctat atatcaggca gaagtacaat gatagggagt gtcttgaact atgtgggtct
      601 aagacttctt ggagaaactt catcacccaa tgacaatggt gatggtgcgc ttgctaaagg
      661 ccgtaaatgg atacttgatc acggtggcgc tacttcgatt ccttcgtggg gcaaggtgta
      721 tctctcggcg cttggagtgt atgaatgggc aggctgcaac cctcttcctc cagaattctg
      781 gcttttccct tcctttttgc cttatcatcc tgcaaaaatg tggtgttact gcagaaccac
      841 atacatgcca atgtcatact tgtatggtag aagctttcat gggcctatta cagaccttgt
      901 tatatcattg agagaagaga ttcatgtcat tccttaccac cagattaatt ggaacaaaca
      961 acgacataat tgttgcaagg aggatctgta ctacccccac acctacatcc aagatctctt
     1021 atgggatgga cttcattatt ttagcgagcc acttatcaca aaatggccct ttaagaagtt
     1081 aagagagaag ggactccaaa gagttttgga gctaatgcaa tataacgctg aggaaggacg
     1141 ttacataacc atgggttgcg tggaaaaggc tttacagatg atgtgtttct ttgccctgga
     1201 tccaaatgga attgacttca aacgacacct tgctagactg ccggattact tgtggatggc
     1261 ggaggatggt atgaagatgc aaagttttgg tagccagtta tgggattgca ctcttgtgac
     1321 tcaagcaatt ctctcaagtg atatggttga cgagtatgga gattcactaa aaaaagctca
     1381 cttttacttg aaagaatcac agataaaaga gaacccaaaa ggagatttcg caaatatgtg
     1441 tcgccaattt acaaaagggg catggacttt ctccgatcaa gatcaaggtt gggtcgtttc
     1501 agattgcacc gctgaagctt tgaagtgttt aatggcgttg tcgcaaatgc cacaagaaat
     1561 tgtcggagaa aaggctgaag tggagcaatt atatgatgct gtgaatgtcc ttctttacct
     1621 acaaagtcca caaagtggtg gttttgcaat ttgggaggca ccagtcccaa aaccatattt
     1681 agagaaatta aacccttcag aactttttgc ggacatagtg gtggaaagag agcatgttga
     1741 atgtacaggc tccataattc aggctctaca aacgttcaaa gatctacatc cagggcatcg
     1801 tgagaaagaa atcgaagttg ctattgaaaa aggcatacac tttttggaaa acaggcaaca
     1861 agaaaatggt tcatggtatg gttattgggg tatatgttat ctctatggca catattttgt
     1921 gcttcaagga ttagtatctt gtgggaaaac atatgaaaat agtgaagcag ttcgaaaagc
     1981 tgtcaatttt ttcctctcaa cacagaactc agaaggtggt tggggagaga actttgagtc
     2041 atgcccacaa gagaaattta tacctttgga ggggaaccga acaaatttgg tgcaaacttc
     2101 atgggcattg ctcggtcttt tatgtggtgg acaggctgaa agggatcaga caccattaca
     2161 caaggcagca aaattactta taaatgggca aatggatgac ggagattttc ctcaacagga
     2221 aataacggga gtgtacatga agaattgcat gttacattat gcagaatata ggaacacttt
     2281 tcccctatgg gcgctcgctg agtatcgaaa gcgtgtttgg ttgccaaaac aagaaaacgg
     2341 ttcgagagac ctaaggctag gtggaggctc agtgatgata agtctgcgat ggtggatgca
     2401 tgtgtcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcagag ggcacaatcc
     2461 tattccgcgc tatccgacaa tctccaagac attaggtgga gttcagttcg gcgtatggca
     2521 tatgtcgctg gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     2581 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     2641 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     2701 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     2761 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     2821 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     2881 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     2941 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     3001 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     3061 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     3121 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     3181 tctcaagaag atcctttgat cttttctacg gggtctgacg ctctattcaa caaagccgcc
     3241 gtcccgtcaa gtcagcgtaa atgggtaggg ggcttcaaat cgtcccccca tacgatataa
     3301 gttgttacta gtgcttggat tctcaccaat aaaaaacgcc cggcggcaac cgagcgttct
     3361 gaacaaatcc agatggagtt ctgaggtcat tactggatct atcaacagga gtccaagcga
     3421 gctcgatatc aaattacgcc ccgccctgcc actcatcgca gtactgttgt aattcattaa
     3481 gcattctgcc gacatggaag ccatcacaaa cggcatgatg aacctgaatc gccagcggca
     3541 tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt
     3601 tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaga
     3661 cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg
     3721 ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga
     3781 gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc
     3841 atatcaccag ctcaccgtct ttcattgcca tacgaaattc cggatgagca ttcatcaggc
     3901 gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta
     3961 aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa
     4021 atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga
     4081 tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca aaaaatacgc
     4141 ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgtgc cgatcaacgt
     4201 ctcattgata cctgaaacaa aacccatcgt acggccaagg aagtctccaa taactgtgat
     4261 ccaccacaag cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtcatgca
     4321 taatccgcac gcatctggaa taaggaagtg ccattccgcc tgacct
//
