LOCUS       sequence-454877-        3051 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  L0 part - CDS (without STOP codon).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Golubova D, Salmon M, Su H, Tansley C, Kaithakottil GG, Linsmith G, 
            Schudoma C, Swarbreck D, O'Connell MA, Patron NJ
  TITLE     Biosynthesis and bioactivity of anti-inflammatory triterpenoids in 
            Calendula officinalis.
  JOURNAL   Nat Commun. 2025 Jul 28;16(1):6941. doi: 10.1038/s41467-025-62269-w.
  PUBMED    40721615
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-07-28"; volume: "16(1):6941. doi"; pages: " 
            10.1038/s41467-025-62269-w"
COMMENT     Sequence Label: pEPCT0CM0210
FEATURES             Location/Qualifiers
     source          1..3051
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1095..1111)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1095..1111)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1108..1130)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(1283..1871)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1363..1382)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2004..2098)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2119..2778)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2693..2712
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2779..2881)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     2968..2990
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2982..2999
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2983..2999
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ataggtctct aatggatatc aaagaagaat tcaagattct cataaagggg tcccttatca
       61 tatcaatttc tcttagttat tgctattttg tgggaagaag tactcctaaa ggctttccaa
      121 gacttttatt gatccttcct gttcttcttc ttttcatttc aattccacta ttcttcaatt
      181 ccgtacatct catcggagca ctttcgttgt tcatcgcttg gctcgctaat ttcaaacttc
      241 tccttttcgc cttcggaaaa ggaccccttt ccgccccttc gattcccctc tcccaattcc
      301 tttcctttgc atgttttccc attgcaaaga ctcaagtggt atacaaaaat ccttcccaca
      361 agtccctttt taattatgga atcaaagcta tccttttcgc ggttttcctt agggtttttt
      421 tttatcatgg tgatcatatg aacccaataa tcgcttgggt tttgttcgcg ttctcagtct
      481 actttggtct ggagctcaca ctagctgtga gctccgtcat aatgggctcg ttacttgggg
      541 tcgagctcga cccccagttt aacgagccct atctctccac atctctccag gaattctggg
      601 gcaggaggtg gaacgtgatg gtcaaccgca tcctttaccc gaccatttac accccggttc
      661 atacgctttc aactcgggta attggtcata tgtgggcccc aataccggcg atcttgacca
      721 catttatcgt gtccggactc atgcatgagc ttattttctt ttattatact cgggattggc
      781 caacatggaa tactctactt ttcttttgtc tacacggtgt ctctttggta acggagataa
      841 taatcaaaaa atctgttaac tttaacggga gtttaccccg ttacataacc accccgtttg
      901 tgttttcgtt cgtgttgggg acatcttatt ggttgttttt cccagaactt atgaggtgta
      961 aactcgtcga acgagcgttt gaagaatatc gggctatggc caagatcgta ccaggtgtcg
     1021 ttaacttcga gagacctaag gctaggtgga ggctcagtga tgataagtct gcgatggtgg
     1081 atgcatgtgt catggtcata gctgtttcct gtgtgaaatt gttatccgct cagagggcac
     1141 aatcctattc cgcgctatcc gacaatctcc aagacattag gtggagttca gttcggcgta
     1201 tggcatatgt cgctggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     1261 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     1321 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     1381 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     1441 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     1501 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     1561 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     1621 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     1681 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     1741 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     1801 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     1861 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcta ttcaacaaag
     1921 ccgccgtccc gtcaagtcag cgtaaatggg tagggggctt caaatcgtcc ccccatacga
     1981 tataagttgt tactagtgct tggattctca ccaataaaaa acgcccggcg gcaaccgagc
     2041 gttctgaaca aatccagatg gagttctgag gtcattactg gatctatcaa caggagtcca
     2101 agcgagctcg atatcaaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc
     2161 attaagcatt ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag
     2221 cggcatcagc accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa
     2281 gaagttgtcc atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc
     2341 tgagacgaaa aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta
     2401 acacgccaca tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact
     2461 ccagagcgat gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact
     2521 atcccatatc accagctcac cgtctttcat tgccatacga aattccggat gagcattcat
     2581 caggcgggca agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt
     2641 ctttaaaaag gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga
     2701 ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc
     2761 agtgattttt ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa
     2821 tacgcccggt agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgccgatc
     2881 aacgtctcat tgatacctga aacaaaaccc atcgtacggc caaggaagtc tccaataact
     2941 gtgatccacc acaagcgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtc
     3001 atgcataatc cgcacgcatc tggaataagg aagtgccatt ccgcctgacc t
//
