LOCUS       sequence-454879-        3073 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  L0 part - CDS (without STOP codon).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Golubova D, Salmon M, Su H, Tansley C, Kaithakottil GG, Linsmith G, 
            Schudoma C, Swarbreck D, O'Connell MA, Patron NJ
  TITLE     Biosynthesis and bioactivity of anti-inflammatory triterpenoids in 
            Calendula officinalis.
  JOURNAL   Nat Commun. 2025 Jul 28;16(1):6941. doi: 10.1038/s41467-025-62269-w.
  PUBMED    40721615
REFERENCE   2  (bases 1 to 3073)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-07-28"; volume: "16(1):6941. doi"; pages: " 
            10.1038/s41467-025-62269-w"
COMMENT     Sequence Label: pEPDG1CB0025
FEATURES             Location/Qualifiers
     source          1..3073
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1117..1133)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1117..1133)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1130..1152)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(1305..1893)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1385..1404)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2026..2120)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2141..2800)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2715..2734
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2801..2903)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     2990..3012
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3004..3021
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3005..3021
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 taggtctcta atgaataacc tcataaagat atcattttat gcaaatattt cacttggata
       61 ttgctttctt gtaggcaaga ttgtcccaaa aggtgtccca agactcctta gcatcctccc
      121 tgttgtcatc atcttcctct ctatccctct ctcattaacc tctgttcact gcattggagg
      181 agtttctttc ttcatttcat ggcttgcaaa cttcaaactc atcctttttg ccttcgaaaa
      241 aggcccgctt tcatcgcctt ctatctcact tatgtggttc tttgcaatgg cttgtttccc
      301 aatcgatgtt acacgatcca ataaagggtt ttcaaacccg aaaacaccta gcaaaaaggt
      361 gtttagttat ggtacaaagg gcgtacttct tacccttttc cttcgggttt attataatca
      421 tggtgaggtt atgaacccaa tgttagcatg gtgcttttta gggttctcgg tttacctaat
      481 gctcgagctc ttggtcacac tcagctcagg gattgtacta ttgtttctca cggttgagct
      541 agaccctcag tttgatgagc cttatctttc cacgtccctg caagactttt ggggacgaag
      601 gtggaacatt atggtcaacc ggatccttca tccgaccatt tacgacccga tgctgaaact
      661 atcagatcgg gtcattggtc ggttgtgggc tccggttcca gccattttga ccaccttcgc
      721 agtttctggg ctcatgcatg agctaatttt cttttacttc acccgagctt ggcctacttg
      781 ggaggtgatg cttttctttt gtctacatgg ggtttgtttg gttatggagg taatgtttaa
      841 gaaacttatt aacgttaaat ggagtctacc acgtcaatta acagcaccgt tagtcgtcat
      901 attcgtgttg gcaacgtcct attggctgtt tctccccgaa ttactgaggt gtaaaatgct
      961 tgaaagagcg tttcaagagt acgaggcggt tagcaagtta gcaatgtatg ttattcgaag
     1021 ccccctaata atttcgaagt ataacggttc gagagaccta aggctaggtg gaggctcagt
     1081 gatgataagt ctgcgatggt ggatgcatgt gtcatggtca tagctgtttc ctgtgtgaaa
     1141 ttgttatccg ctcagagggc acaatcctat tccgcgctat ccgacaatct ccaagacatt
     1201 aggtggagtt cagttcggcg tatggcatat gtcgctggaa agaacatgtg agcaaaaggc
     1261 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     1321 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     1381 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     1441 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     1501 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     1561 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     1621 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     1681 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     1741 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     1801 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     1861 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     1921 tctgacgctc tattcaacaa agccgccgtc ccgtcaagtc agcgtaaatg ggtagggggc
     1981 ttcaaatcgt ccccccatac gatataagtt gttactagtg cttggattct caccaataaa
     2041 aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg aggtcattac
     2101 tggatctatc aacaggagtc caagcgagct cgatatcaaa ttacgccccg ccctgccact
     2161 catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg
     2221 catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc
     2281 ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg
     2341 tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga
     2401 aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc
     2461 ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa
     2521 cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac
     2581 gaaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact
     2641 tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt
     2701 tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg
     2761 atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg
     2821 aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt
     2881 tggaacctct tacgtgccga tcaacgtctc attgatacct gaaacaaaac ccatcgtacg
     2941 gccaaggaag tctccaataa ctgtgatcca ccacaagcgc cagggttttc ccagtcacga
     3001 cgttgtaaaa cgacggccag tcatgcataa tccgcacgca tctggaataa ggaagtgcca
     3061 ttccgcctga cct
//
