LOCUS       sequence-45488-e         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Sims JJ, Scavone F, Cooper EM, Kane LA, Youle RJ, Boeke JD, Cohen RE
  TITLE     Polyubiquitin-sensor proteins reveal localization and linkage-type 
            dependence of cellular ubiquitin signaling.
  JOURNAL   Nat Methods. 2012 Feb 5. doi: 10.1038/nmeth.1888.
  PUBMED    22306808
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Feb 5. doi: 10.1038/nmeth.1888."
COMMENT     Sequence Label: pQCXIP-Rx3(A7)-mEGFP
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      245..254
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(375..399)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(396..599)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(425..445)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gtggcgaccg gtggatcccc agaaggccgg caactattca gactttctgc aatggctttc
       61 ctcaagagct cttcttcctc ctcggcagcc gccgctgcgg cagcagcttc ctgttcactc
      121 attttgagag ccagagcaaa ctgttcttct tcctcttctg cggcagcagc ggcagccgcc
      181 gcctcttgct cgctcatctt cagtgcgagt gcgaattgct cctcctcggt catatggctg
      241 ccctccatgg tggcggcgcg gccgcggatc aattcctgca ggtccgttcc aatgcaccgt
      301 tcccggccgc ggaggctgga tcggtcccgg tgtcttctat ggaggtcaaa acagcgtgga
      361 tggcgtctcc aggcgatctg acggttcact aaacgagctc tgcttatata gacctcccac
      421 cgtacacgcc taccgcccat ttgcgtcaat ggggcggagt tgttacgaca ttttggaaag
      481 tcccgttgat tttggtgcca aaacaaactc ccattgacgt caatggggtg gagacttgga
      541 aatccccgtg agtcaaaccg ctatccacgc ccattgatgt actgccaaaa ccgcatcacc
      601 atggtaatag cgatgactaa tacgtagatg tactgccaag taggaaagtc ccataaggtc
      661 atgtactggg cataatgcca ggcgggccat ttaccgtcat tgacgtcaat agggggcgta
      721 cttggcatat gatacacttg atgtactgcc aagtgggcag tttaccgtaa atactccacc
      781 cattgacgtc aatggaaagt ccctattggc gttactatgg gaacatacgt cattattgac
//
