LOCUS       sequence-454880-        3076 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  L0 part - CDS (without STOP codon).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Golubova D, Salmon M, Su H, Tansley C, Kaithakottil GG, Linsmith G, 
            Schudoma C, Swarbreck D, O'Connell MA, Patron NJ
  TITLE     Biosynthesis and bioactivity of anti-inflammatory triterpenoids in 
            Calendula officinalis.
  JOURNAL   Nat Commun. 2025 Jul 28;16(1):6941. doi: 10.1038/s41467-025-62269-w.
  PUBMED    40721615
REFERENCE   2  (bases 1 to 3076)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-07-28"; volume: "16(1):6941. doi"; pages: " 
            10.1038/s41467-025-62269-w"
COMMENT     Sequence Label: pEPDG1CB0024
FEATURES             Location/Qualifiers
     source          1..3076
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1120..1136)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1120..1136)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1133..1155)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(1308..1896)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1388..1407)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2029..2123)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2144..2803)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2718..2737
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2804..2906)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     2993..3015
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3007..3024
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3008..3024
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 taggtctcta atgatgaata acctcatcaa gatatcattt tatgcatata tttcacttgg
       61 gtattgcttt cttgttggca agattgtccc aaaaggtgtc ccaagactcc ttagtatcct
      121 ccctgtcgtc atcatcttcc tctctatccc tctatcttta acatccgttc actgcattgg
      181 agccgtttct ttcttcattt catggcttgc aaacttcaaa ctcatccttt tcgccttcga
      241 aaaaggcccc ctttcatcac cttctatatc acttatgtgg ttctttgcaa tggcttgctt
      301 cccaatccaa gtttcacggt ccaataaagg gttttcaacc ccgaaaacac ctagaaaaaa
      361 agtgtttagt tatggtacaa agggcgtact tcttaccctt ttccttcggg tttattataa
      421 ccatggtgcg gttatgaacc cgatgctagc atggtgtttc ttagggttct cgctttacct
      481 aatgctcgag ctcttgctca tactcagctc agggattgta ctattgtttc tcacgattga
      541 gctagacccc caatttgacg agccttatct ttccacgtcc ctacaagact tttggggacg
      601 aaggtggaac attatggtca accggatcct tcatccgacc atttacgacc cgattcttaa
      661 actatcagat cgggtcgtgg gtcggttgtg ggctccggtt ccagccattt tgaccacctt
      721 cgcagtttct gggctcatgc atgagctcat tttcttttac ttcacccgag cttggcctac
      781 ttgggaggtg atgcttttct tttgtctaca tggggtttgt ttggtcacgg aggtaatgtt
      841 taagaaactt cttaacgtta aatggagtct accacgtcaa ttaacagcac cgttagtcgt
      901 catattcgtg ttggcaacgt cgtgttggtt gtttctcccg gaattactaa ggtgtaaaat
      961 gcttgaaagg gcgtttcaag agtacgaggc ggttagcaag ttagcaatgg atgttattca
     1021 aagtccccta atatttttga agtataatgg ttcgagagac ctaaggctag gtggaggctc
     1081 agtgatgata agtctgcgat ggtggatgca tgtgtcatgg tcatagctgt ttcctgtgtg
     1141 aaattgttat ccgctcagag ggcacaatcc tattccgcgc tatccgacaa tctccaagac
     1201 attaggtgga gttcagttcg gcgtatggca tatgtcgctg gaaagaacat gtgagcaaaa
     1261 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     1321 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     1381 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     1441 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     1501 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     1561 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     1621 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     1681 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     1741 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     1801 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     1861 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     1921 gggtctgacg ctctattcaa caaagccgcc gtcccgtcaa gtcagcgtaa atgggtaggg
     1981 ggcttcaaat cgtcccccca tacgatataa gttgttacta gtgcttggat tctcaccaat
     2041 aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc agatggagtt ctgaggtcat
     2101 tactggatct atcaacagga gtccaagcga gctcgatatc aaattacgcc ccgccctgcc
     2161 actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaaa
     2221 cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt
     2281 tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac
     2341 tggtgaaact cacccaggga ttggctgaga cgaaaaacat attctcaata aaccctttag
     2401 ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact
     2461 gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga
     2521 aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca
     2581 tacgaaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa
     2641 acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct
     2701 ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt
     2761 gggatatatc aacggtggta tatccagtga tttttttctc cattttagct tccttagctc
     2821 ctgaaaatct cgataactca aaaaatacgc ccggtagtga tcttatttca ttatggtgaa
     2881 agttggaacc tcttacgtgc cgatcaacgt ctcattgata cctgaaacaa aacccatcgt
     2941 acggccaagg aagtctccaa taactgtgat ccaccacaag cgccagggtt ttcccagtca
     3001 cgacgttgta aaacgacggc cagtcatgca taatccgcac gcatctggaa taaggaagtg
     3061 ccattccgcc tgacct
//
