LOCUS       sequence-454884-        3105 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  L0 part - CDS (without STOP codon).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Golubova D, Salmon M, Su H, Tansley C, Kaithakottil GG, Linsmith G, 
            Schudoma C, Swarbreck D, O'Connell MA, Patron NJ
  TITLE     Biosynthesis and bioactivity of anti-inflammatory triterpenoids in 
            Calendula officinalis.
  JOURNAL   Nat Commun. 2025 Jul 28;16(1):6941. doi: 10.1038/s41467-025-62269-w.
  PUBMED    40721615
REFERENCE   2  (bases 1 to 3105)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-07-28"; volume: "16(1):6941. doi"; pages: " 
            10.1038/s41467-025-62269-w"
COMMENT     Sequence Label: pEPCT0CM0207
FEATURES             Location/Qualifiers
     source          1..3105
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1149..1165)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1149..1165)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1162..1184)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(1337..1925)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1417..1436)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2058..2152)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2173..2832)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2747..2766
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2833..2935)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     3022..3044
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3036..3053
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3037..3053
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ataggtctct aatggatgga gaggtgcaca acttcatagt ggtttggata atagtgttgc
       61 taggacttct ctattcttac accataggca agttcatccc aaaaggtaca attagacttt
      121 tggctttatc cattgttata tgcctctttc tatggtttcc actcaatcta accaccatac
      181 accttggtgg tttaacctct ttctttatat catggcttgc aaacttcaaa cttcttctct
      241 atgcttatgg aaaaggtccc ctttcttcgg atccatcgat ctcgttgtct agctttatcc
      301 tcctggcgtg cctgccaatt cggtccgtcc atacttcatc ttcttcatac cctgaaaagc
      361 cgactcaaaa aaccaagata aatgagaaaa tagttttatc gaaggtttta ttgtttggga
      421 tgttacttaa agggtatgat tattatgaat atattcatcc gaagttgata atggtgttgt
      481 atagcatcca tatctacttc atgcttgagt tcgtgcttgc catgctcgca tttgtggctc
      541 gaaccctagt gcaaatggat ttagagccac aatttgacga gccttatctt gctacttccc
      601 tccaagactt ttggggcaaa aggtggaacc taatggtcac tagaattcta catccgacta
      661 tatatgaacc tattcattca atttctacgc gttttatacc aagaaaatgg gcatctctag
      721 ttgcggtttt tgcatccttt cttgtttcgg gtttgatgca cgagttcata ttctataaca
      781 ttggtagact gaaaccgacc ggagaagtaa catgtttttt cttattacat ggagtattga
      841 tgagcattga agttgttatc aagagagcaa caaagggaaa attacatttg catcccgttg
      901 tgtcgaggcc aataactttg ggatgcgtgc tcattacatg tttttggttg ttctttccgc
      961 cgtttttgcg gtttaaacca gacatccgtg gttgtcaaga aaccgttgca tttctaaagt
     1021 ttgtaacaaa cggtcaacta attagcccta gtaacgcttc ttgtccgtat ctatcaagtt
     1081 tcgagagacc taaggctagg tggaggctca gtgatgataa gtctgcgatg gtggatgcat
     1141 gtgtcatggt catagctgtt tcctgtgtga aattgttatc cgctcagagg gcacaatcct
     1201 attccgcgct atccgacaat ctccaagaca ttaggtggag ttcagttcgg cgtatggcat
     1261 atgtcgctgg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     1321 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     1381 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     1441 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     1501 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     1561 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     1621 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     1681 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     1741 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     1801 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     1861 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     1921 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tctattcaac aaagccgccg
     1981 tcccgtcaag tcagcgtaaa tgggtagggg gcttcaaatc gtccccccat acgatataag
     2041 ttgttactag tgcttggatt ctcaccaata aaaaacgccc ggcggcaacc gagcgttctg
     2101 aacaaatcca gatggagttc tgaggtcatt actggatcta tcaacaggag tccaagcgag
     2161 ctcgatatca aattacgccc cgccctgcca ctcatcgcag tactgttgta attcattaag
     2221 cattctgccg acatggaagc catcacaaac ggcatgatga acctgaatcg ccagcggcat
     2281 cagcaccttg tcgccttgcg tataatattt gcccatggtg aaaacggggg cgaagaagtt
     2341 gtccatattg gccacgttta aatcaaaact ggtgaaactc acccagggat tggctgagac
     2401 gaaaaacata ttctcaataa accctttagg gaaataggcc aggttttcac cgtaacacgc
     2461 cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt cactccagag
     2521 cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca
     2581 tatcaccagc tcaccgtctt tcattgccat acgaaattcc ggatgagcat tcatcaggcg
     2641 ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa
     2701 aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa
     2761 tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat atccagtgat
     2821 ttttttctcc attttagctt ccttagctcc tgaaaatctc gataactcaa aaaatacgcc
     2881 cggtagtgat cttatttcat tatggtgaaa gttggaacct cttacgtgcc gatcaacgtc
     2941 tcattgatac ctgaaacaaa acccatcgta cggccaagga agtctccaat aactgtgatc
     3001 caccacaagc gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtcatgcat
     3061 aatccgcacg catctggaat aaggaagtgc cattccgcct gacct
//
