LOCUS       sequence-454885-        3472 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  L0 part - CDS (without STOP codon).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3472)
  AUTHORS   Golubova D, Salmon M, Su H, Tansley C, Kaithakottil GG, Linsmith G, 
            Schudoma C, Swarbreck D, O'Connell MA, Patron NJ
  TITLE     Biosynthesis and bioactivity of anti-inflammatory triterpenoids in 
            Calendula officinalis.
  JOURNAL   Nat Commun. 2025 Jul 28;16(1):6941. doi: 10.1038/s41467-025-62269-w.
  PUBMED    40721615
REFERENCE   2  (bases 1 to 3472)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-07-28"; volume: "16(1):6941. doi"; pages: " 
            10.1038/s41467-025-62269-w"
COMMENT     Sequence Label: pEPMS0CM0042
FEATURES             Location/Qualifiers
     source          1..3472
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1516..1532)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1516..1532)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1529..1551)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(1704..2292)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1784..1803)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2425..2519)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2540..3199)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3114..3133
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(3200..3302)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     3389..3411
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3403..3420
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3404..3420
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 taggtctcta atggatatct tctatgcttc tctcgtatct ctcttcgtta ttgctgtttc
       61 attctcaatt catttggtgt tctacaagtc taagccacat gtagaaggca aactgccacc
      121 tggccaaaaa gggtggccgg tgatcggaga aagtatcgag tttctggcca ccggatggaa
      181 aggccaccct gaaaagttca tcttcgaacg catggctaaa ttctcacctc aagttttcag
      241 gacctctctc atgttagaag atgcggccgt gttttgcggt gcttctggta acaaattttt
      301 gttctcaaat gagaacaaac ttgttcaagc atggtggcca tctaatgtca acaagatctt
      361 tccatcttca aaccaatcat cacaaatcga agccaaaaag atgaaaagtt tgcttcctaa
      421 tttcttcaaa ccggaagcct tacgtggata cgttccaatc atggacaaag tgacacaaaa
      481 acactttgaa accgggtgga aaggcaagga ccaaatcgtg acatacgaac tcaccaagaa
      541 cttcactttt tggcttgcat gcaagatctt tgtcagtatt gatgaccccg aacgggtcaa
      601 gtatctgggt ggcccgttcg agagcatcgc tctagggatc ctctcgatcc ctatcgacct
      661 ccccggaaca ccgttccgga gagggattaa tgcagctaac ttcattagga aagaactaac
      721 tgcaataata aagcaaagaa agacggattt ggctaatggt aaagcttcac ctacacaaga
      781 catattgtca cacatgcttc tttataggga tgaagatggt aaattcatgg aagaacatga
      841 tattgccgac aagatcctcg gattgttaat aggtggccat gataccgcaa gctcggcatg
      901 cgcattcatt gtcaagtatc tggctgagct accagaaatt tacgagggag tctacaaaga
      961 acaaaccgaa attgcaaaat ctaaagcgcc aggagaattg ttgaactggg atgatttatc
     1021 gaagatgaag tactcttgga atgtggcatg tgaagttctt agattagccc cacctctcca
     1081 aggtgctttc agggaagcta ttactgattt tatgtacaat ggctactcca ttcccaaggg
     1141 ctggaagttg tattggagtg caaactcggt tcataaaaac cccgagttct ttcccgaacc
     1201 acaaaagttt gacccgtcca gattcgatgg gaaaggacca ataccatata catttgtacc
     1261 gtttggagga gggcctcgta tgtgccctgg aaaagagtat gcacgattag aaatacttgt
     1321 gttcatgcat catctcgtaa gaaagtataa gtgggagaaa gtaattcccg atgaacaaat
     1381 tgttgtcaac ccgatgccga gccccgaaaa aggacttccg attcgcttat atcctcataa
     1441 tgctggttcg agagacctaa ggctaggtgg aggctcagtg atgataagtc tgcgatggtg
     1501 gatgcatgtg tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcagagggca
     1561 caatcctatt ccgcgctatc cgacaatctc caagacatta ggtggagttc agttcggcgt
     1621 atggcatatg tcgctggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     1681 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     1741 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     1801 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     1861 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     1921 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     1981 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     2041 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     2101 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     2161 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     2221 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     2281 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctct attcaacaaa
     2341 gccgccgtcc cgtcaagtca gcgtaaatgg gtagggggct tcaaatcgtc cccccatacg
     2401 atataagttg ttactagtgc ttggattctc accaataaaa aacgcccggc ggcaaccgag
     2461 cgttctgaac aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc
     2521 aagcgagctc gatatcaaat tacgccccgc cctgccactc atcgcagtac tgttgtaatt
     2581 cattaagcat tctgccgaca tggaagccat cacaaacggc atgatgaacc tgaatcgcca
     2641 gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga
     2701 agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg
     2761 ctgagacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt
     2821 aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac
     2881 tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac
     2941 tatcccatat caccagctca ccgtctttca ttgccatacg aaattccgga tgagcattca
     3001 tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg
     3061 tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg
     3121 actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc
     3181 cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat aactcaaaaa
     3241 atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgccgat
     3301 caacgtctca ttgatacctg aaacaaaacc catcgtacgg ccaaggaagt ctccaataac
     3361 tgtgatccac cacaagcgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt
     3421 catgcataat ccgcacgcat ctggaataag gaagtgccat tccgcctgac ct
//
