LOCUS       sequence-454894-       12178 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing the DHX9 transcription factor 
            ORF with a unique 24-bp barcode. Barcodes facilitate identification 
            of transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12178)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 12178)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0025
FEATURES             Location/Qualifiers
     source          1..12178
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    8035..8623
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8088..8108)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8506..8517)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8695..8928
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9006..9127
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9043..9062)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9097..9116
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9167..9302
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9322..9341)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9323..9341)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9351..9368)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9351..9367)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9360..9382)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9509..9964
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9596..9615)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9806..9827
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9990..10094
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10095..10163,10164..10955)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10313..10332)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11126..11714
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11615..11634
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11868..11885
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12002..12023
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12038..12043,12044..12061,12062..12068)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12076..12092
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12081..12103
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12100..12116
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12100..12116
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12135..12155
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12137..12155
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgggt gacgttaaaa attttctgta
     4141 tgcctggtgt ggcaaaagga agatgacccc atcctatgaa attagagcag tggggaacaa
     4201 aaacaggcag aaattcatgt gtgaggttca ggtggaaggt tataattaca ctggcatggg
     4261 aaattccacc aataaaaaag atgcacaaag caatgctgcc agagactttg ttaactattt
     4321 ggttcgaata aatgaaataa agagtgaaga agttccagct tttggggtag catctccgcc
     4381 cccacttact gatactcctg acactacagc aaatgctgaa ggagatttac caacaaccat
     4441 gggaggacct cttcctccac atctggctct caaagcagaa aataattctg aggtaggggc
     4501 ctctggctat ggtgttcctg ggcccacctg ggaccgagga gccaacttga aggattacta
     4561 ctcaagaaag gaagaacaag aagtgcaagc gactctagaa tcagaagaag tggatttaaa
     4621 tgctgggctt catggaaact ggaccttgga aaatgctaaa gctcgtctaa accaatattt
     4681 tcagaaagaa aagatccaag gagaatataa gtacacccaa gtgggtcctg atcacaacag
     4741 gagctttatt gcagaaatga ccatttatat caagcagctg ggcagaagga tttttgcacg
     4801 agaacatgga tcaaataaga aattggcagc acagtcctgt gccctgtcac ttgtcagaca
     4861 actgtaccat cttggagtgg ttgaagctta ctccggactt acaaagaaga aggaaggaga
     4921 gacagtggag ccttacaaag taaacctctc tcaagattta gagcatcagc tgcaaaacat
     4981 cattcaagag ctaaatcttg agattttgcc cccgcctgaa gatccttctg tgccagttgc
     5041 actcaacatt ggcaaattgg ctcagttcga accatctcag cgacaaaacc aagtgggtgt
     5101 ggttccttgg tcacctccac aatccaactg gaatccttgg actagtagca acattgatga
     5161 ggggcctctg gcttttgcta ctccagagca aataagcatg gacctcaaga atgaattgat
     5221 gtaccagttg gaacaggatc atgatttgca agcaatcttg caggagagag agttactgcc
     5281 tgtgaagaaa tttgaaagtg agattctgga agcaatcagc caaaattcag ttgtcattat
     5341 tagaggggct actggatgtg ggaaaaccac acaggttccc cagttcattc tagatgactt
     5401 tatccagaat gaccgagcag cagagtgtaa catcgtagta actcagccca gaagaatcag
     5461 tgcggtttct gtggcagagc gagttgcatt tgaaagagga gaagagcctg gaaaaagctg
     5521 tggctacagc gttcgatttg agtctatact tcctcgtcct catgccagta taatgttttg
     5581 tactgtaggt gtgctcctga gaaaattaga agcaggcatt cgaggaatca gtcatgtaat
     5641 tgtagatgaa atacatgaaa gagatattaa tactgacttc cttttggtag tactgcgtga
     5701 tgttgttcag gcttatcctg aagttcgcat tgttcttatg tctgctacta ttgataccag
     5761 catgttttgt gaatatttct tcaattgccc catcattgaa gtttatggga ggacttaccc
     5821 agttcaagaa tattttctgg aagactgcat tcagatgacc cactttgttc ctccaccaaa
     5881 agacaaaaag aagaaggata aggatgatga tggtggtgag gatgatgatg caaattgcaa
     5941 cttgatctgt ggtgatgaat atggtccaga aacaaggttg agcatgtctc aattgaacga
     6001 aaaggaaact ccttttgaac tcatcgaggc tctacttaag tacattgaaa cccttaatgt
     6061 tcctggagct gtgttggttt ttttgcctgg ctggaatctg atttatacta tgcagaagca
     6121 tttggaaatg aatccacatt ttggaagcca tcggtatcag attctacccc tgcattctca
     6181 gattcctcga gaggaacagc gcaaagtgtt tgatccagta ccagttggag taaccaaggt
     6241 tattttgtcc acaaatattg ctgaaacaag cattaccata aacgatgttg tttatgtcat
     6301 tgactcctgc aagcagaaag tgaaactctt cactgctcac aacaatatga ccaactatgc
     6361 taccgtatgg gcatcaaaaa caaaccttga gcaacggaaa gggcgagctg gccgagtacg
     6421 gcctggattc tgctttcacc tgtgcagccg agctcgtttt gagagacttg aaacccacat
     6481 gacaccagag atgttccgaa caccattgca tgaaattgct cttagcataa aacttctgcg
     6541 tctaggagga attggccaat ttctggccaa agcaattgaa cctccccctt tggatgctgt
     6601 gattgaagca gaacacactc ttagagagct tgatgcatta gatgccaatg atgagttgac
     6661 tcctttggga cgaatcctgg ctaaactccc cattgagcct cgttttggca aaatgatgat
     6721 aatggggtgt attttctacg tgggagatgc tatctgtacc attgctgctg ctacctgctt
     6781 tccagagcct ttcatcaatg aaggaaagcg gctgggctat atccatcgaa attttgctgg
     6841 aaacagattt tctgatcacg tagccctttt atcagtattc caagcctggg atgatgctag
     6901 aatgggtgga gaagaagcag agatacgttt ttgtgagcac aaaagactta atatggctac
     6961 actaagaatg acttgggaag ccaaagttca gctcaaagag attttgatta attctgggtt
     7021 tccagaagat tgtttgttga cacaagtgtt tactaacact ggaccagata ataatttgga
     7081 tgttgttatc tccctcctgg cctttggtgt gtaccccaat gtatgctatc ataaggaaaa
     7141 gaggaagatt ctcaccactg aagggcgtaa tgcacttatc cacaaatcat ctgttaattg
     7201 tccttttagt agccaagaca tgaagtaccc atctcccttc tttgtatttg gtgaaaagat
     7261 tcgaactcga gccatctctg ctaaaggcat gactttagtc acccccctgc agttgcttct
     7321 ctttgcctcc aagaaagtcc aatctgatgg gcagattgtg cttgtagatg actggattaa
     7381 actgcaaata tctcatgaag ctgctgcctg tatcactggt ctccgggcag ccatggaggc
     7441 tttggttgtt gaagtaacca aacaacctgc tatcatcagc cagttggacc ccgtaaatga
     7501 acgtatgctg aacatgatcc gtcagatctc tagaccctca gctgctggta tcaaccttat
     7561 gattggcagt acacggtatg gagatggtcc acgtcctccc aagatggccc gatacgacaa
     7621 tggaagcgga tatagaaggg gaggttctag ttacagtggt ggaggctatg gcggtggcta
     7681 tagcagtgga ggctatggta gcggaggcta tggtggcagc gccaactcct ttcgggcagg
     7741 atatggtgca ggtgttggtg gaggctatag aggagtttcc cgaggtggct ttagaggcaa
     7801 ctctggagga gactacagag ggcctagtgg aggctacaga ggatctgggg gattccagcg
     7861 aggaggtggt aggggggcct atggaactgg ctactttgga cagggaagag gaggtggcgg
     7921 ctattagtaa tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc
     7981 ttgtggaaag gacgaacccg acacgacgcg ggcattcaca cgcgttaagt cgacaatcaa
     8041 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     8101 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     8161 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     8221 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     8281 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     8341 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     8401 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     8461 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     8521 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     8581 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcgtcgact ttaagaccaa
     8641 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     8701 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     8761 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     8821 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     8881 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt acgtatagta
     8941 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     9001 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     9061 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     9121 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac
     9181 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     9241 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     9301 cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
     9361 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     9421 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     9481 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
     9541 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     9601 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     9661 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     9721 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     9781 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     9841 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     9901 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     9961 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
    10021 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
    10081 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
    10141 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
    10201 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
    10261 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
    10321 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
    10381 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
    10441 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
    10501 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
    10561 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
    10621 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
    10681 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
    10741 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
    10801 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
    10861 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
    10921 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
    10981 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
    11041 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
    11101 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
    11161 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
    11221 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
    11281 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
    11341 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
    11401 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
    11461 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
    11521 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
    11581 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
    11641 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
    11701 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
    11761 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
    11821 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
    11881 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
    11941 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
    12001 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
    12061 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
    12121 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagc
//
