LOCUS       sequence-454922-       13015 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13015)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 13015)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2086
FEATURES             Location/Qualifiers
     source          1..13015
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    8872..9460
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8925..8945)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9343..9354)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9532..9765
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9843..9964
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9880..9899)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9934..9953
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10004..10139
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10159..10178)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10160..10178)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10188..10205)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10188..10204)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10197..10219)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10346..10801
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10433..10452)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10643..10664
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10827..10931
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10932..11000,11001..11792)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11150..11169)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11963..12551
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12452..12471
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12705..12722
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12839..12860
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12875..12880,12881..12898,12899..12905)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12913..12929
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12918..12940
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12937..12953
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12937..12953
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12972..12992
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12974..12992
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgccc tcggacttca tctcattgct
     4141 cagcgcggac ctagacctgg aatcgcccaa gtccctctac tcgcgagatt ctctgaagtt
     4201 acacccatca cagaattttc atagagctgg actattggaa gaatctgtct atgatcttct
     4261 cccaaaggag ttacagttac ctccatctag agaaacatct gtagcatcaa tgagtcagac
     4321 aagcggtggt gaggcaggct cgcctcctcc agctgttgtt gctgctgatg cttcttcagc
     4381 tccctcctct tcctccatgg gcggtgcttg cagctccttt accacctctt ccagccctac
     4441 catttattct acctcagtca ccgacagcaa ggctatgcaa gtggagagct gctcctcagc
     4501 cgtgggggta agtaacagag gggtaagtga aaagcagtta accagtaaca cagttcagca
     4561 gcatccatca acaccgaaga ggcacacagt cttgtacatc tcaccaccac ctgaggactt
     4621 gctggataac agtcggatgt cctgccagga tgaggggtgt ggattggaat ctgagcagag
     4681 ctgcagtatg tggatggagg attccccctc caacttcagt aacatgagca ccagttccta
     4741 caatgataac actgaggtac ctcgtaaatc acgaaaacga aatccaaagc agaggccggg
     4801 ggtcaaacga cgagattgtg aagaatctaa tatggatata tttgatgccg acagtgccaa
     4861 agcacctcac tatgtgcttt ctcagcttac cacggacaac aaaggcaact caaaagcggg
     4921 aaatggaaca ttggaaaacc aaaaaggaac tggagtaaag aagagcccta tgttgtgtgg
     4981 acaatatcct gttaaaagtg agggaaagga gctgaagata gttgtacaac ctgagacaca
     5041 gcaccgagct cggtacctga ctgagggcag ccgtggctca gtgaaagata gaacacagca
     5101 aggctttcct acagtaaagc tggaaggcca taatgaacct gtagtgttgc aagtgtttgt
     5161 gggcaacgac tctggacgag tgaaaccaca tggattttat caggcctgca gagtaactgg
     5221 acgaaataca actccttgca aagaagtgga cattgaaggc actactgtta tagaagtcgg
     5281 ccttgatcct agcaacaaca tgacactggc ggtggactgc gtagggatat tgaaattgag
     5341 gaatgctgat gtcgaagcca gaataggaat tgctggttcc aagaagaaaa gcactcgtgc
     5401 cagattggtt tttcgagtta atatcatgag gaaagatggc tccactttga cactgcaaac
     5461 accctcttct ccaattttgt gtactcagcc agcaggagtg ccagaaatct taaagaaaag
     5521 cttgcatagc tgttcagtga aaggagaaga agaagtgttt ttaatcggca agaactttct
     5581 gaaaggaact aaagttattt tccaagaaaa tgtttctgat gaaaactctt ggaagtcaga
     5641 agctgaaatt gatatggaac tatttcatca gaatcatctt attgtgaagg ttcctcccta
     5701 tcatgaccaa catataactt tgcctgtgtc agtgggaata tatgtagtga caaatgctgg
     5761 aagatctcat gatgttcaac cattcactta cactccagac ccagcagcag ctggtgcttt
     5821 gaatgtaaat gtgaagaagg aaatatctag tccagcaaga ccttgctctt ttgaagaggc
     5881 catgaaagca atgaaaacta ctggatgtaa tttagataag gtaaatatta tccctaatgc
     5941 cctgatgact ccactcatac caagcagtat gattaagagt gaagatgtta ctccaatgga
     6001 agtaacagca gaaaaaagat cttccactat ttttaagact acaaagtctg ttggatcaac
     6061 tcagcaaaca ttagaaaaca tctcaaacat agcaggaaat ggctcttttt catcaccatc
     6121 atcttcccac ctaccttctg aaaatgaaaa acagcagcag attcagccca aggcatacaa
     6181 cccagagacc ctgacaacta ttcaaaccca ggacatctca cagcctggta cttttccagc
     6241 agtttctgct tctagtcagc tgcccaacag cgatgcacta ttgcagcagg ctacacagtt
     6301 tcagacaaga gaaactcagt ctagagagat attacagtca gatggtacag tggttaattt
     6361 gtcacaactg actgaggcat cacaacaaca gcagcagtca ccactacaag aacaagcaca
     6421 gactttacag cagcagattt catcaaatat ttttccatca ccaaatagtg tgagtcagct
     6481 tcagaatact attcagcagc tgcaagcagg gagtttcaca ggcagtactg ctagtggcag
     6541 cagtggaagt gttgacttgg tccaacaagt tttagaggca cagcagcagt tatcttcagt
     6601 tttattttct gctccagatg gtaatgagaa tgttcaagag cagcttagtg cagatatttt
     6661 tcaacaagtc agtcaaattc agagtggtgt aagccctgga atgttttcct caacagagcc
     6721 aacagtccat accagaccag ataatttatt acctggaaga gctgaaagtg ttcatccaca
     6781 gtctgaaaac acgttatcta atcaacagca gcagcagcag cagcaacagc aagtgatgga
     6841 atcttcagcc gcaatggtga tggagatgca acagagtatc tgccaggcag ctgcccagat
     6901 tcagtcagag ttattccctt caactgcttc agcaaatgga aaccttcagc aatcgccagt
     6961 ttaccagcag acttctcaca tgatgagtgc attgtctacc aatgaggata tgcaaatgca
     7021 gtgtgaattg ttttcttctc ctcctgcagt ttctggaaat gaaacttcta caactaccac
     7081 acagcaggtt gcaacccctg gcactaccat gtttcagaca tcaagttcag gagatggaga
     7141 agaaactgga acacaagcaa aacagattca gaacagtgtc tttcagacca tggtccaaat
     7201 gcaacatagt ggggacaatc aacctcaagt taaccttttt tcatccacaa aaagtatgat
     7261 gagtgttcag aatagtggta cccaacaaca aggtaatggt ttattccagc aagggaatga
     7321 gatgatgtca cttcaatctg gaaatttttt gcagcagtct tctcattcac aggcccaact
     7381 ttttcatcct caaaatccta ttgccgatgc tcagaacctt tcccaggaaa ctcaaggttc
     7441 tctctttcat agtccaaatc ctattgtcca cagtcagact tctacaacct cctctgaaca
     7501 aatgcagcct ccaatgtttc actctcaaag taccattgct gtgttacagg gctcttcagt
     7561 tcctcaagac cagcagtcaa ccaacatatt tctttcccag agtcccatga ataatcttca
     7621 gactaacaca gtagcccaag aagcattttt tgcagcaccg aactcaattt ctccacttca
     7681 gtcaacatca aacagtgaac aacaagctgc tttccaacag caagctccaa tatcacacat
     7741 ccagactcct atgctttccc aagaacaggc acaacccccg cagcagggtt tatttcagcc
     7801 tcaggtggcc ctgggctccc ttccacctaa tccaatgcct caaagccaac aaggaaccat
     7861 gttccagtca cagcactcaa tagttgccat gcagagtaac tctccatccc aggaacagca
     7921 gcagcagcag caacagcagc agcaacagca gcagcaacaa caacagagca ttttattcag
     7981 taatcagaat accatggcta caatggcgtc tccaaagcaa ccaccaccaa acatgatatt
     8041 caacccaaat caaaatccaa tggctaatca ggagcaacag aaccagtcaa tttttcacca
     8101 acaaagtaac atggccccaa tgaatcaaga gcaacagccc atgcaatttc agagtcagtc
     8161 cacagtttcc tcacttcaga acccaggtcc tacccagtcg gaatcatcac agaccccctt
     8221 gttccatagc tctcctcaga ttcagttggt acaagggtca cctagttctc aagagcagca
     8281 agtaactctc ttcttatctc cagcatccat gtctgccttg cagaccagta taaatcaaca
     8341 agatatgcaa cagtctcctc tttattcccc tcagaacaac atgcctggaa ttcaaggagc
     8401 cacatcttcg cctcaaccac aggctacttt atttcacaac acagcaggag gcacaatgaa
     8461 ccaactgcag aattctcctg gctcatctca gcagacatca ggaatgttct tatttggcat
     8521 tcaaaataac tgtagtcagc ttttaacctc tggaccagct acattgcctg atcagttgat
     8581 ggccataagt cagccaggcc aaccacaaaa cgagggccag ccacctgtga caacacttct
     8641 ttctcagcaa atgccagaga attctccact ggcatcctct ataaacacca accagaacat
     8701 cgaaaagatt gatttgcttg tttcattgca aaaccaaggg aacaacttga ctggctcctt
     8761 ttagtaatga actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg
     8821 tggaaaggac gaactgcgca cagagagcgt atcctaacgc gttaagtcga caatcaacct
     8881 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     8941 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     9001 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     9061 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     9121 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     9181 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     9241 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     9301 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     9361 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     9421 cctcagacga gtcggatctc cctttgggcc gcctccccgc gtcgacttta agaccaatga
     9481 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     9541 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     9601 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     9661 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     9721 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtacg tatagtagtt
     9781 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
     9841 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     9901 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     9961 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc
    10021 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
    10081 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
    10141 agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt
    10201 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
    10261 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
    10321 ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
    10381 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
    10441 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
    10501 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
    10561 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
    10621 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
    10681 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
    10741 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
    10801 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
    10861 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
    10921 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
    10981 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
    11041 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
    11101 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
    11161 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
    11221 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
    11281 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
    11341 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
    11401 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
    11461 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
    11521 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
    11581 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
    11641 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
    11701 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
    11761 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
    11821 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
    11881 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
    11941 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
    12001 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
    12061 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
    12121 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
    12181 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
    12241 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
    12301 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
    12361 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
    12421 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
    12481 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
    12541 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
    12601 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
    12661 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
    12721 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
    12781 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
    12841 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
    12901 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
    12961 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagc
//
