LOCUS       sequence-454923-       12781 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12781)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 12781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2090
FEATURES             Location/Qualifiers
     source          1..12781
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    8638..9226
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8691..8711)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9109..9120)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9298..9531
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9609..9730
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9646..9665)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9700..9719
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9770..9905
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9925..9944)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9926..9944)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9954..9971)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9954..9970)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9963..9985)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10112..10567
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10199..10218)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10409..10430
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10593..10697
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10698..10766,10767..11558)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10916..10935)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11729..12317
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12218..12237
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12471..12488
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12605..12626
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12641..12646,12647..12664,12665..12671)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12679..12695
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12684..12706
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12703..12719
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12703..12719
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12738..12758
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12740..12758
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggtt tccaaaagaa gactgtcaaa
     4141 atctgaggat aaagagagcc tgacagaaga tgcctccaaa accaggaagc aaccactttc
     4201 caaaaagaca aagaaatctc atattgctaa tgaagttgaa gaaaatgaca gcatctttgt
     4261 aaagcttctt aagatatcag gaattattct taaaacggga gagagtcaga atcaactagc
     4321 tgtggatcaa atagctttcc aaaagaagct ctttcagacc ctgaggagac acccttccta
     4381 tcccaaaata atagaagaat ttgttagtgg cctggagtct tacattgagg atgaagacag
     4441 tttcaggaac tgccttttgt cttgtgagcg tctgcaggat gaggaagcca gtatgggtgc
     4501 atcttattct aagagtctca tcaaactgct tctggggatt gacatactgc agcctgccat
     4561 tatcaaaacc ttatttgaga agttgccaga atattttttt gaaaacaaga acagtgatga
     4621 aatcaacata cctcgactca ttgtcagtca actaaaatgg cttgacagag ttgtggatgg
     4681 caaggacctc accaccaaga tcatgcagct gatcagtatt gctccagaga acctgcagca
     4741 tgacatcatc accagcctac ctgagatcct aggggattcc cagcacgctg atgtggggaa
     4801 agaactcagt gacctactga tagagaatac ttcactcact gtcccaatcc tggatgtcct
     4861 ttcaagcctc cgacttgacc caaacttcct attgaaggtt cgccagttgg tgatggataa
     4921 gttgtcgtct attagattgg aggatttacc tgtgataata aagttcattc ttcattccgt
     4981 aacagccatg gatacacttg aggtaatttc tgagcttcgg gagaagttgg atctgcagca
     5041 ttgtgttttg ccatcacggt tacaggcttc ccaagtaaag ttgaaaagta aaggacgagc
     5101 aagttcctca ggaaatcaag aaagcagcgg tcagagctgt attattctcc tctttgatgt
     5161 aataaagtca gctattagat atgagaaaac catttcagaa gcctggatta aggcaattga
     5221 aaacactgcc tcagtatctg aacacaaggt gtttgacctg gtgatgcttt tcatcatcta
     5281 tagcaccaat actcagacaa agaagtacat tgacagggtg ctaagaaata agattcgatc
     5341 aggctgcatt caagaacagc tgctccagag tacattctct gttcattact tagttcttaa
     5401 ggatatgtgt tcatccattc tgtcgctggc tcagagtttg cttcactctc tagaccagag
     5461 tataatttca tttggcagtc tcctatacaa atatgcattt aagttttttg acacgtactg
     5521 ccagcaggaa gtggttggtg ccttagtgac ccatatctgc agtgggaatg aagctgaagt
     5581 tgatactgcc ttagatgtcc ttctagagtt ggtagtgtta aacccatctg ctatgatgat
     5641 gaatgctgtc tttgtaaagg gcattttaga ttatctggat aacatatccc ctcagcaaat
     5701 acgaaaactc ttctatgttc tcagcacact ggcatttagc aaacagaatg aagccagcag
     5761 ccacatccag gatgacatgc acttggtgat aagaaagcag ctctctagca ccgtattcaa
     5821 gtacaagctc attgggatta ttggtgctgt gaccatggct ggcatcatgg cggcagacag
     5881 aagtgaatca cctagtttga cccaagagag agccaacctg agcgatgagc agtgcacaca
     5941 ggtgacctcc ttgttgcagt tggttcattc ctgcagtgag cagtctcctc aggcctctgc
     6001 actttactat gatgaatttg ccaacctgat ccaacatgaa aagctggatc caaaagccct
     6061 ggaatgggtt gggcatacca tctgtaatga tttccaggat gccttcgtag tggactcctg
     6121 tgttgttccg gaaggtgact ttccatttcc tgtgaaagca ctgtacggac tggaagaata
     6181 cgacactcag gatgggattg ccataaacct cctgccgctg ctgttttctc aggactttgc
     6241 aaaagatggg ggtccggtga cctcacagga atcaggccaa aaattggtgt ctccgctgtg
     6301 cctggctccg tatttccggt tactgagact ttgtgtggag agacagcata acggaaactt
     6361 ggaggagatt gatggtctac tagattgtcc tatattccta actgacctgg agcctggaga
     6421 gaagttggag tccatgtctg ctaaagagcg ttcattcatg tgttctctca tatttcttac
     6481 tctcaactgg ttccgagaga ttgtaaatgc cttctgccag gaaacatcac ctgagatgaa
     6541 ggggaaggtg ctcactcggt taaagcacat tgtagaattg caaataatcc tggaaaagta
     6601 cttggcagtc accccagact atgtccctcc tcttggaaac tttgatgtgg aaactttaga
     6661 tataacacct catactgtta ctgctatttc agcaaaaatc agaaagaaag gaaaaataga
     6721 aaggaaacaa aaaacagatg gcagcaagac atcctcctct gacacacttt cagaagagaa
     6781 aaattcagaa tgtgacccta cgccatctca tagaggccag ctaaacaagg agttcacagg
     6841 gaaggaagaa aagacatcat tgttactaca taattcccat gcttttttcc gagagctgga
     6901 cattgaggtc ttctctattc tacattgtgg acttgtgacg aagttcatct tagatactga
     6961 aatgcacact gaagctacag aagttgtgca acttgggccc cctgagctgc ttttcttgct
     7021 ggaagatctc tcccagaagc tggagagtat gctgacacct cctattgcca ggagagtccc
     7081 ctttctcaag aacaaaggaa gccggaatat tggattctca catctccaac agagatctgc
     7141 ccaagaaatt gttcattgtg tttttcaact gctgacccca atgtgtaacc acctggagaa
     7201 cattcacaac tattttcagt gtttagctgc tgagaatcac ggtgtagttg atggaccagg
     7261 agtgaaagtt caggagtacc acataatgtc ttcctgctat cagaggctgc tgcagatttt
     7321 tcatgggctt tttgcttgga gtggattttc tcaacctgaa aatcagaatt tactgtattc
     7381 agccctccat gtccttagta gccgactgaa acagggagaa cacagccagc ctttggagga
     7441 actactcagc cagagcgtcc attacttgca gaatttccat caaagcattc ccagtttcca
     7501 gtgtgctctt tatctcatca gacttttgat ggttattttg gagaaatcaa cagcttctgc
     7561 tcagaacaaa gaaaaaattg cttcccttgc cagacaattc ctctgtcggg tgtggccaag
     7621 tggggataaa gagaagagca acatctctaa tgaccagctc catgctctgc tctgtatcta
     7681 cctggagcac acagagagca ttctgaaggc catagaggag attgctggtg ttggtgtccc
     7741 agaactgatc aactctccta aagatgcatc ttcctccaca ttccctacac tgaccaggca
     7801 tacttttgtt gttttcttcc gtgtgatgat ggctgaacta gagaagacgg tgaaaaaaat
     7861 tgagcctggc acagcagcag actcgcagca gattcatgaa gagaaactcc tctactggaa
     7921 catggctgtt cgagacttca gtatcctcat caacttgata aaggtatttg atagtcatcc
     7981 tgttctgcat gtatgtttga agtatgggcg tctctttgtg gaagcatttc tgaagcaatg
     8041 tatgccgctc ctagacttca gttttagaaa acaccgggaa gatgttctga gcttactgga
     8101 aaccttccag ttggacacaa ggctgcttca tcacctgtgt gggcattcca agattcacca
     8161 ggacacgaga ctcacccaac atgtgcctct gctcaaaaag accctggaac ttttagtttg
     8221 cagagtcaaa gctatgctca ctctcaacaa ttgtagagag gctttctggc tgggcaatct
     8281 aaaaaaccgg gacttgcagg gtgaagagat taagtcccaa aattcccagg agagcacagc
     8341 agatgagagt gaggatgaca tgtcatccca ggcctccaag agcaaagcca ctgaggtatc
     8401 tctacaaaac ccaccagagt ctggcactga tggttgcatt ttgttaattg ttctaagttg
     8461 gtggagcaga actttgccta cttatgttta ttgtcaaatg cttctatgcc catttccatt
     8521 ccctccatag taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat
     8581 atcttgtgga aaggacgagc attgacacgc ggcaggcaca gaacgcgtta agtcgacaat
     8641 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     8701 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     8761 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     8821 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     8881 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     8941 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     9001 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     9061 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     9121 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     9181 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcgtcg actttaagac
     9241 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     9301 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
     9361 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     9421 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     9481 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtacgtata
     9541 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     9601 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     9661 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     9721 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct
     9781 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     9841 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     9901 aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
     9961 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
    10021 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
    10081 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
    10141 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
    10201 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
    10261 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
    10321 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
    10381 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
    10441 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
    10501 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
    10561 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
    10621 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
    10681 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
    10741 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
    10801 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
    10861 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
    10921 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
    10981 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
    11041 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
    11101 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
    11161 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
    11221 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
    11281 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
    11341 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
    11401 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
    11461 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
    11521 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
    11581 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
    11641 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
    11701 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
    11761 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
    11821 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
    11881 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
    11941 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
    12001 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
    12061 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
    12121 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
    12181 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
    12241 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
    12301 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
    12361 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
    12421 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
    12481 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
    12541 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
    12601 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
    12661 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
    12721 atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag
    12781 c
//
