LOCUS       sequence-454928-       11734 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11734)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 11734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2471
FEATURES             Location/Qualifiers
     source          1..11734
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    7591..8179
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7644..7664)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8062..8073)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8251..8484
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8562..8683
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8599..8618)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8653..8672
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8723..8858
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8878..8897)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8879..8897)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8907..8924)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8907..8923)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8916..8938)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9065..9520
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9152..9171)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9362..9383
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9546..9650
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9651..9719,9720..10511)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9869..9888)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10682..11270
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11171..11190
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11424..11441
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11558..11579
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11594..11599,11600..11617,11618..11624)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11632..11648
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11637..11659
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11656..11672
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11656..11672
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11691..11711
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11693..11711
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgaac tctcccaacg agtcggatgg
     4141 gatgtcaggt cgggaaccat ccttggaaat cctgccgcgg acttctctgc acagcatccc
     4201 tgtgacagtg gaggtgaagc cggtgctgcc aagagccatg cccagttcca tggggggtgg
     4261 gggtggaggc agccccagcc ctgtggagct acggggggct ctggtgggct ctgtggaccc
     4321 cacactgcgg gagcagcaac tgcagcagga gctcctggcg ctcaagcagc agcagcagct
     4381 gcagaagcag ctcctgttcg ctgagttcca gaaacagcat gaccacctga caaggcagca
     4441 tgaggtccag ctgcagaagc acctcaagca gcagcaggag atgctggcag ccaagcagca
     4501 gcaggagatg ctggcagcca agcggcagca ggagctggag cagcagcggc agcgggagca
     4561 gcagcggcag gaagagctgg agaagcagcg gctggagcag cagctgctca tcctgcggaa
     4621 caaggagaag agcaaagaga gtgccattgc cagcactgag gtaaagctga ggctccagga
     4681 attcctcttg tcgaagtcaa aggagcccac accaggcggc ctcaaccatt ccctcccaca
     4741 gcaccccaaa tgctggggag cccaccatgc ttctttggac cagagttccc ctccccagag
     4801 cggcccccct gggacgcctc cctcctacaa actgcctttg cctgggccct acgacagtcg
     4861 agacgacttc cccctccgca aaacagcctc tgaacccaac ttgaaagtgc gttcaaggct
     4921 aaaacagaag gtggctgagc ggagaagcag tcccctcctg cgtcgcaagg atgggactgt
     4981 tattagcacc tttaagaaga gagctgttga gatcacaggt gccgggcctg gggcgtcgtc
     5041 cgtgtgtaac agcgcacccg gctccggccc cagctctccc aacagctccc acagcaccat
     5101 cgctgagaat ggctttactg gctcagtccc caacatcccc actgagatgc tccctcagca
     5161 ccgagccctc cctctggaca gctcccccaa ccagttcagc ctctacacgt ctccttctct
     5221 gcccaacatc tccctagggc tgcaggccac ggtcactgtc accaactcac acctcactgc
     5281 ctccccgaag ctgtcgacac agcaggaggc cgagaggcag gccctccagt ccctgcggca
     5341 gggtggcacg ctgaccggca agttcatgag cacatcctct attcctggct gcctgctggg
     5401 cgtggcactg gagggcgacg ggagccccca cgggcatgcc tccctgctgc agcatgtgct
     5461 gttgctggag caggcccggc agcagagcac cctcattgct gtgccactcc acgggcagtc
     5521 cccactagtg acgggtgaac gtgtggccac cagcatgcgg acggtaggca agctcccgcg
     5581 gcatcggccc ctgagccgca ctcagtcctc accgctgccg cagagtcccc aggccctgca
     5641 gcagctggtc atgcaacaac agcaccagca gttcctggag aagcagaagc agcagcagct
     5701 acagctgggc aagatcctca ccaagacagg ggagctgccc aggcagccca ccacccaccc
     5761 tgaggagaca gaggaggagc tgacggagca gcaggaggtc ttgctggggg agggagccct
     5821 gaccatgccc cgggagggct ccacagagag tgagagcaca caggaagacc tggaggagga
     5881 ggacgaggaa gacgatgggg aggaggagga ggattgcatc caggttaagg acgaggaggg
     5941 cgagagtggt gctgaggagg ggcccgactt ggaggagcct ggtgctggat acaaaaaact
     6001 gttctcagat gcccagccgc tgcagccttt gcaggtgtac caggcgcccc tcagcctggc
     6061 cactgtgccc caccaggccc tgggccgtac ccagtcctcc cctgctgccc ctgggggcat
     6121 gaagagcccc ccagaccagc ccgtcaagca cctcttcacc acaggtgtgg tctacgacac
     6181 gttcatgcta aagcaccagt gcatgtgcgg gaacacacac gtgcaccctg agcatgctgg
     6241 ccggatccag agcatctggt cccggctgca ggagacaggc ctgcttagca agtgcgagcg
     6301 gatccgaggt cgcaaagcca cgctagatga gatccagaca gtgcactctg aataccacac
     6361 cctgctctat gggaccagtc ccctcaaccg gcagaagcta gacagcaaga agttgctcgg
     6421 ccccatcagc cagaagatgt atgctgtgct gccttgtggg ggcatcgggg tggacagtga
     6481 caccgtgtgg aatgagatgc actcctccag tgctgtgcgc atggcagtgg gctgcctgct
     6541 ggagctggcc ttcaaggtgg ctgcaggaga gctcaagaat ggatttgcca tcatccggcc
     6601 cccaggacac cacgccgagg aatccacagc catgggattc tgcttcttca actctgtagc
     6661 catcaccgca aaactcctac agcagaagtt gaacgtgggc aaggtcctca tcgtggactg
     6721 ggacattcac catggcaatg gcacccagca ggcgttctac aatgacccct ctgtgctcta
     6781 catctctctg catcgctatg acaacgggaa cttctttcca ggctctgggg ctcctgaaga
     6841 ggttggtgga ggaccaggcg tggggtacaa tgtgaacgtg gcatggacag gaggtgtgga
     6901 cccccccatt ggagacgtgg agtaccttac agccttcagg acagtggtga tgcccattgc
     6961 ccacgagttc tcacctgatg tggtcctagt ctccgccggg tttgatgctg ttgaaggaca
     7021 tctgtctcct ctgggtggct actctgtcac cgccagatgt tttggccact tgaccaggca
     7081 gctgatgacc ctggcagggg gccgggtggt gctggccctg gagggaggcc atgacttgac
     7141 cgccatctgt gatgcctctg aggcttgtgt ctcggctctg ctcagtgtag agctgcagcc
     7201 cttggatgag gcagtcttgc agcaaaagcc caacatcaac gcagtggcca cgctagagaa
     7261 agtcatcgag atccagagca aacactggag ctgtgtgcag aagttcgccg ctggtctggg
     7321 ccggtccctg cgagaggccc aagcaggtga gaccgaggag gccgagactg tgagcgccat
     7381 ggccttgctg tcggtggggg ccgagcaggc ccaggctgcg gcagcccggg aacacagccc
     7441 caggccggca gaggagccca tggagcagga gcctgccctg tagtaatgaa ctagtccgta
     7501 acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg atttagctga
     7561 taggatattg tcgaaacgcg ttaagtcgac aatcaacctc tggattacaa aatttgtgaa
     7621 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     7681 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     7741 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     7801 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     7861 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     7921 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     7981 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     8041 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     8101 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     8161 ctttgggccg cctccccgcg tcgactttaa gaccaatgac ttacaaggca gctgtagatc
     8221 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     8281 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     8341 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     8401 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     8461 agtcagtgtg gaaaatctct agcagtacgt atagtagttc atgtcatctt attattcagt
     8521 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
     8581 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     8641 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
     8701 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     8761 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     8821 gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc
     8881 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa
     8941 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     9001 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     9061 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     9121 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     9181 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     9241 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     9301 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     9361 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     9421 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     9481 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
     9541 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     9601 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     9661 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     9721 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     9781 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     9841 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     9901 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     9961 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
    10021 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
    10081 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
    10141 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
    10201 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
    10261 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
    10321 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
    10381 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
    10441 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
    10501 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
    10561 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
    10621 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
    10681 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
    10741 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
    10801 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
    10861 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
    10921 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
    10981 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
    11041 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
    11101 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
    11161 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
    11221 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
    11281 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
    11341 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
    11401 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
    11461 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
    11521 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
    11581 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
    11641 gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc
    11701 ctcactaaag ggaacaaaag ctggagctgc aagc
//
