LOCUS       sequence-454929-       11737 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11737)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 11737)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2472
FEATURES             Location/Qualifiers
     source          1..11737
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    7594..8182
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7647..7667)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8065..8076)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8254..8487
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8565..8686
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8602..8621)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8656..8675
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8726..8861
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8881..8900)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8882..8900)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8910..8927)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8910..8926)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8919..8941)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9068..9523
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9155..9174)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9365..9386
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9549..9653
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9654..9722,9723..10514)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9872..9891)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10685..11273
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11174..11193
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11427..11444
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11561..11582
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11597..11602,11603..11620,11621..11627)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11635..11651
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11640..11662
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11659..11675
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11659..11675
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11694..11714
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11696..11714
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgaac tctcccaacg agtcggcaga
     4141 tgggatgtca ggtcgggaac catccttgga aatcctgccg cggacttctc tgcacagcat
     4201 ccctgtgaca gtggaggtga agccggtgct gccaagagcc atgcccagtt ccatgggggg
     4261 tgggggtgga ggcagcccca gccctgtgga gctacggggg gctctggtgg gctctgtgga
     4321 ccccacactg cgggagcagc aactgcagca ggagctcctg gcgctcaagc agcagcagca
     4381 gctgcagaag cagctcctgt tcgctgagtt ccagaaacag catgaccacc tgacaaggca
     4441 gcatgaggtc cagctgcaga agcacctcaa gcagcagcag gagatgctgg cagccaagca
     4501 gcagcaggag atgctggcag ccaagcggca gcaggagctg gagcagcagc ggcagcggga
     4561 gcagcagcgg caggaagagc tggagaagca gcggctggag cagcagctgc tcatcctgcg
     4621 gaacaaggag aagagcaaag agagtgccat tgccagcact gaggtaaagc tgaggctcca
     4681 ggaattcctc ttgtcgaagt caaaggagcc cacaccaggc ggcctcaacc attccctccc
     4741 acagcacccc aaatgctggg gagcccacca tgcttctttg gaccagagtt cccctcccca
     4801 gagcggcccc cctgggacgc ctccctccta caaactgcct ttgcctgggc cctacgacag
     4861 tcgagacgac ttccccctcc gcaaaacagc ctctgaaccc aacttgaaag tgcgttcaag
     4921 gctaaaacag aaggtggctg agcggagaag cagtcccctc ctgcgtcgca aggatgggac
     4981 tgttattagc acctttaaga agagagctgt tgagatcaca ggtgccgggc ctggggcgtc
     5041 gtccgtgtgt aacagcgcac ccggctccgg ccccagctct cccaacagct cccacagcac
     5101 catcgctgag aatggcttta ctggctcagt ccccaacatc cccactgaga tgctccctca
     5161 gcaccgagcc ctccctctgg acagctcccc caaccagttc agcctctaca cgtctccttc
     5221 tctgcccaac atctccctag ggctgcaggc cacggtcact gtcaccaact cacacctcac
     5281 tgcctccccg aagctgtcga cacagcagga ggccgagagg caggccctcc agtccctgcg
     5341 gcagggtggc acgctgaccg gcaagttcat gagcacatcc tctattcctg gctgcctgct
     5401 gggcgtggca ctggagggcg acgggagccc ccacgggcat gcctccctgc tgcagcatgt
     5461 gctgttgctg gagcaggccc ggcagcagag caccctcatt gctgtgccac tccacgggca
     5521 gtccccacta gtgacgggtg aacgtgtggc caccagcatg cggacggtag gcaagctccc
     5581 gcggcatcgg cccctgagcc gcactcagtc ctcaccgctg ccgcagagtc cccaggccct
     5641 gcagcagctg gtcatgcaac aacagcacca gcagttcctg gagaagcaga agcagcagca
     5701 gctacagctg ggcaagatcc tcaccaagac aggggagctg cccaggcagc ccaccaccca
     5761 ccctgaggag acagaggagg agctgacgga gcagcaggag gtcttgctgg gggagggagc
     5821 cctgaccatg ccccgggagg gctccacaga gagtgagagc acacaggaag acctggagga
     5881 ggaggacgag gaagacgatg gggaggagga ggaggattgc atccaggtta aggacgagga
     5941 gggcgagagt ggtgctgagg aggggcccga cttggaggag cctggtgctg gatacaaaaa
     6001 actgttctca gatgcccagc cgctgcagcc tttgcaggtg taccaggcgc ccctcagcct
     6061 ggccactgtg ccccaccagg ccctgggccg tacccagtcc tcccctgctg cccctggggg
     6121 catgaagagc cccccagacc agcccgtcaa gcacctcttc accacaggtg tggtctacga
     6181 cacgttcatg ctaaagcacc agtgcatgtg cgggaacaca cacgtgcacc ctgagcatgc
     6241 tggccggatc cagagcatct ggtcccggct gcaggagaca ggcctgctta gcaagtgcga
     6301 gcggatccga ggtcgcaaag ccacgctaga tgagatccag acagtgcact ctgaatacca
     6361 caccctgctc tatgggacca gtcccctcaa ccggcagaag ctagacagca agaagttgct
     6421 cggccccatc agccagaaga tgtatgctgt gctgccttgt gggggcatcg gggtggacag
     6481 tgacaccgtg tggaatgaga tgcactcctc cagtgctgtg cgcatggcag tgggctgcct
     6541 gctggagctg gccttcaagg tggctgcagg agagctcaag aatggatttg ccatcatccg
     6601 gcccccagga caccacgccg aggaatccac agccatggga ttctgcttct tcaactctgt
     6661 agccatcacc gcaaaactcc tacagcagaa gttgaacgtg ggcaaggtcc tcatcgtgga
     6721 ctgggacatt caccatggca atggcaccca gcaggcgttc tacaatgacc cctctgtgct
     6781 ctacatctct ctgcatcgct atgacaacgg gaacttcttt ccaggctctg gggctcctga
     6841 agaggttggt ggaggaccag gcgtggggta caatgtgaac gtggcatgga caggaggtgt
     6901 ggaccccccc attggagacg tggagtacct tacagccttc aggacagtgg tgatgcccat
     6961 tgcccacgag ttctcacctg atgtggtcct agtctccgcc gggtttgatg ctgttgaagg
     7021 acatctgtct cctctgggtg gctactctgt caccgccaga tgttttggcc acttgaccag
     7081 gcagctgatg accctggcag ggggccgggt ggtgctggcc ctggagggag gccatgactt
     7141 gaccgccatc tgtgatgcct ctgaggcttg tgtctcggct ctgctcagtg tagagctgca
     7201 gcccttggat gaggcagtct tgcagcaaaa gcccaacatc aacgcagtgg ccacgctaga
     7261 gaaagtcatc gagatccaga gcaaacactg gagctgtgtg cagaagttcg ccgctggtct
     7321 gggccggtcc ctgcgagagg cccaagcagg tgagaccgag gaggccgaga ctgtgagcgc
     7381 catggccttg ctgtcggtgg gggccgagca ggcccaggct gcggcagccc gggaacacag
     7441 ccccaggccg gcagaggagc ccatggagca ggagcctgcc ctgtagtaat gaactagtcc
     7501 gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg acgacgaatt
     7561 tcagtcgaca cattcctgac gcgttaagtc gacaatcaac ctctggatta caaaatttgt
     7621 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     7681 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     7741 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     7801 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     7861 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     7921 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     7981 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     8041 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     8101 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     8161 tccctttggg ccgcctcccc gcgtcgactt taagaccaat gacttacaag gcagctgtag
     8221 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     8281 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     8341 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     8401 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     8461 tttagtcagt gtggaaaatc tctagcagta cgtatagtag ttcatgtcat cttattattc
     8521 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag
     8581 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     8641 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct
     8701 agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     8761 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     8821 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg
     8881 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg
     8941 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     9001 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     9061 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     9121 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     9181 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     9241 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     9301 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     9361 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     9421 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     9481 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg
     9541 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     9601 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     9661 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     9721 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     9781 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     9841 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     9901 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     9961 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
    10021 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
    10081 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
    10141 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
    10201 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
    10261 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
    10321 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
    10381 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
    10441 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
    10501 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
    10561 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
    10621 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
    10681 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
    10741 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
    10801 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
    10861 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
    10921 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
    10981 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
    11041 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
    11101 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
    11161 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
    11221 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
    11281 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
    11341 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
    11401 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
    11461 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
    11521 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
    11581 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
    11641 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta
    11701 accctcacta aagggaacaa aagctggagc tgcaagc
//
