LOCUS       sequence-454983-       11974 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11974)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 11974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0248
FEATURES             Location/Qualifiers
     source          1..11974
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    7831..8419
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7884..7904)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8302..8313)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8491..8724
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8802..8923
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8839..8858)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8893..8912
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8963..9098
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9118..9137)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9119..9137)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9147..9164)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9147..9163)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9156..9178)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9305..9760
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9392..9411)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9602..9623
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9786..9890
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9891..9959,9960..10751)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10109..10128)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10922..11510
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11411..11430
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11664..11681
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11798..11819
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11834..11839,11840..11857,11858..11864)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11872..11888
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11877..11899
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11896..11912
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11896..11912
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11931..11951
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11933..11951
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgaat accaaggaca ccaccgaggt
     4141 tgctgaaaac agccaccacc tgaagatctt tctccccaag aagctgctgg agtgtcttcc
     4201 tcgctgcccg ctgctgcctc cagagaggct acggtggaat acaaatgagg agattgcatc
     4261 ctacctgatc acctttgaga agcatgatga gtggctgtct tgtgccccaa agacaaggcc
     4321 tcagaatggc tccatcatcc tctacaatcg caagaaggtg aaatatcgga aggatggtta
     4381 cctctggaag aagcggaagg atgggaagac cacccgagag gaccacatga agctgaaggt
     4441 ccagggcatg gagtgtctct atggctgcta cgttcactct tccatcgtcc ccacattcca
     4501 tcggcgctgc tactggctgc tccagaaccc tgacatcgtc cttgtgcact acctgaacgt
     4561 cccagccctg gaggactgtg gaaagggctg cagccccatc ttttgttcca tcagcagcga
     4621 ccgtcgagag tggctgaagt ggtcccggga ggagttgttg ggacagctga agcccatgtt
     4681 tcatggcatc aagtggagct gcgggaatgg aacagaagag ttctctgtag aacacctggt
     4741 gcagcagatt ttggacaccc acccaaccaa gcctgctccc cgaacccacg cctgtctctg
     4801 cagtgggggg cttggttctg ggagccttac ccacaaatgc agcagcacga aacaccgcat
     4861 catctctccc aaagtggagc cccgagcttt aaccctgacc tctatccccc acgctcaccc
     4921 cccagagcct cctccactga tagccccact tcccccagag ctccccaagg cacacacctc
     4981 cccatcttct tcctcttctt cctcctcatc aggttttgca gagcccctag aaatcagacc
     5041 tagccctccc acttctcgag ggggttcttc aagaggaggc actgctatcc tcctcctgac
     5101 aggactggag cagcgggctg gaggcttgac gcccaccagg cacttggctc cacaggctga
     5161 tcctaggcct tccatgagtt tggcagtggt tgtaggcact gagccttctg ccccaccagc
     5221 tcctcccagt cctgcctttg accctgatcg ttttctcaac agcccccaga ggggccagac
     5281 atatggaggg gggcagggag taagcccaga cttccccgag gcagaggccg ctcatacccc
     5341 ctgttctgcc ctagagcctg ctgctgccct ggagccccag gcagctgctc ggggtccccc
     5401 accacagtca gtagcaggtg ggagaagagg aaactgcttc ttcatccaag atgatgacag
     5461 tggggaggag ctcaagggtc acggggctgc cccacccata ccttcacccc ctccctcacc
     5521 cccaccctca cctgccccct tggagccgtc aagcagggta ggaagaggag aggccttgtt
     5581 tggaggacct gttggggcca gtgaactgga gcccttcagt ctttcatcat tcccagacct
     5641 tatgggagaa ctcatcagtg acgaagctcc aagcatccct gctccgaccc cccagctgtc
     5701 tcctgctctt agcaccatca cagacttctc cccagagtgg tcctacccag agggtggggt
     5761 caaggtgctc atcacaggtc cttggaccga agccgccgag cattactcct gtgtctttga
     5821 tcacatcgca gtgccagcct cacttgtcca gcctggtgtc ttacgctgct actgtcccgc
     5881 ccatgaggta gggctggtgt ctttgcaggt ggcagggcgg gaggggcccc tttctgcttc
     5941 tgtgctcttt gagtatcgag cccgccgatt cctgtctctg cctagtactc aacttgactg
     6001 gctgtcactg gacgacaacc agttccggat gtccatacta gagcgactgg agcagatgga
     6061 gaagcggatg gcagagatcg cagcagctgg gcaggtgcct tgccagggtc ctgatgctcc
     6121 tccagttcag gatgaaggcc aggggcctgg gttcgaagca cgggtagtgg tcttggtaga
     6181 aagcatgatc ccacgctcca cctggaaggg tcctgaacgt ctggcccatg gaagcccctt
     6241 ccggggcatg agccttctgc acctggctgc tgcccagggc tatgcccgcc tcatcgagac
     6301 cctgagccag tggcggagtg tggagactgg aagcttggac ttagagcagg aggttgaccc
     6361 gctcaacgtg gatcatttct cttgcacccc tctgatgtgg gcttgtgccc tgggacacct
     6421 ggaagctgct gtgctccttt tccgttggaa ccgacaggca ctgagcattc ccgactctct
     6481 gggccgtctg ccattgtctg tggctcattc ccggggtcat gtgcgccttg cccgctgcct
     6541 tgaggaacta cagagacagg agccttcggt ggagccccca tttgccctat cgccaccctc
     6601 ctccagccca gacactggtc tgagcagcgt ctcctcgccc tcggagctgt cggatggcac
     6661 cttttccgtc acgtcagcct attctagtgc cccagatggc agtccccccc ctgcacctct
     6721 gccagcctct gagatgacta tggaggacat ggccccaggc cagctttcct ctggtgtccc
     6781 agaagccccc ctactcctca tggactatga ggctaccaac tccaaggggc ccctctcctc
     6841 ccttcctgcc ctcccaccag cttcagatga tggggctgct ccagaggacg ctgacagccc
     6901 acaggctgtg gatgtgatcc cggtggacat gatctcacta gccaagcaga tcatcgaagc
     6961 cacaccggag cggattaaac gagaggactt cgtggggctg cccgaggctg gagcctcaat
     7021 gcgggagcgg acaggggctg tggggctcag tgagaccatg tcctggctgg ccagctacct
     7081 ggagaatgtg gaccatttcc ccagctcaac ccctcccagc gaactgccct ttgagcgagg
     7141 tcgcctggct gtcccttcag caccctcctg ggcagagttt ctctctgcat ccaccagtgg
     7201 caagatggaa agtgattttg ccctgctgac actatcagat cacgagcagc gggaactgta
     7261 tgaggctgcc cgagtcatcc agacggcctt ccgaaagtac aagggccggc ggctgaagga
     7321 gcagcaggag gtagcagcag ctgtaatcca gcgctgttac cggaagtaca agcagctgac
     7381 ctggattgca cttaagtttg cactctataa gaagatgacc caggcggcca tcctgatcca
     7441 gagcaagttc cgaagctact atgaacagaa gcgatttcag cagagccgcc gagcggctgt
     7501 gctcatccag cagcactacc gctcctaccg ccgcaggccc ggccctcccc accggacttc
     7561 ggccaccctg cctgcccgca acaaaggctc ctttctcacc aagaagcagg accaggcagc
     7621 ccggaagatc atgagattcc tgcggcgctg ccgacacagg atgagggaac tgaagcagaa
     7681 ccaggagctg gaagggcttc cccagccggg actggccaca tagtaatgaa ctagtccgta
     7741 acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg aggtataggc
     7801 cttcaaacgc tagccacgcg ttaagtcgac aatcaacctc tggattacaa aatttgtgaa
     7861 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     7921 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     7981 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     8041 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     8101 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     8161 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     8221 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     8281 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     8341 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     8401 ctttgggccg cctccccgcg tcgactttaa gaccaatgac ttacaaggca gctgtagatc
     8461 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     8521 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     8581 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     8641 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     8701 agtcagtgtg gaaaatctct agcagtacgt atagtagttc atgtcatctt attattcagt
     8761 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
     8821 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     8881 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
     8941 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     9001 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     9061 gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc
     9121 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa
     9181 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     9241 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     9301 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     9361 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     9421 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     9481 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     9541 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     9601 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     9661 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     9721 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
     9781 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     9841 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     9901 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     9961 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
    10021 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
    10081 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
    10141 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
    10201 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
    10261 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
    10321 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
    10381 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
    10441 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
    10501 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
    10561 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
    10621 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
    10681 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
    10741 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
    10801 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
    10861 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
    10921 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
    10981 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
    11041 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
    11101 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
    11161 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
    11221 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
    11281 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
    11341 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
    11401 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
    11461 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
    11521 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
    11581 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
    11641 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
    11701 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
    11761 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
    11821 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
    11881 gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc
    11941 ctcactaaag ggaacaaaag ctggagctgc aagc
//
