LOCUS       sequence-455000-        4165 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CMV promoter driving expression of adenoviral E4orf6 gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4165)
  AUTHORS   Doshi J, Couto E, Staiti J, Vandenberghe LH, Zabaleta N
  TITLE     E2A, VA RNA I, and L4-22k adenoviral helper genes are sufficient for
            AAV production in HEK293 cells.
  JOURNAL   Mol Ther Methods Clin Dev. 2024 Nov 13;32(4):101376. doi: 
            10.1016/j.omtm.2024.101376. eCollection 2024 Dec 12.
  PUBMED    39670178
REFERENCE   2  (bases 1 to 4165)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther 
            Methods Clin Dev."; date: "2024-11-13"; volume: "32(4):101376. doi";
            pages: " 10.1016/j.omtm.2024.101376. eCollection 2024 Dec 12"
COMMENT     Sequence Label: JD92
FEATURES             Location/Qualifiers
     source          1..4165
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        254..272
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     254..271
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     enhancer        341..644
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        645..848
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     799..819
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    1819..2043
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(2127..2146)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2128..2146)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        2238..2342
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2343..2411,2412..3203)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2561..2580)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3401..3989
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3890..3909
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4143..4160
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgaaaaatca ataatcagac aacaagatgt gcgaactcga tattttacac gactctcttt
       61 accaattctg ccccgaatta cacttaaaac gactcaacag cttaacgttg gcttgccacg
      121 cattacttga ctgtaaaact ctcactctta ccgaacttgg ccgtaacctg ccaaccaaag
      181 cgagaacaaa acataacatc aaacgaatcg accgattgtt aggtaatcgt cacctgcagg
      241 aaggtttaaa cgcatttagg tgacactata gaagtgtgta tcgctcgagg gatccgaatt
      301 cgaagtcttg gtacggagcg agaccggagc gagacgggag cgttacataa cttacggtaa
      361 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
      421 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
      481 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
      541 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
      601 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
      661 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
      721 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
      781 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
      841 gcagagctca ctgtgactga ttgagctggt gccgtgtcga gtggtgtttt ttaataggtt
      901 tttttactgg aaatatgact acgtccggcg ttccatttgg catgacacta cgaccaacac
      961 gatctcggtt gtctcggcgc actccgtaca gtagggatcg cctacctcct tttgagacag
     1021 agacccgcgc taccatactg gaggatcatc cgctgctgcc cgaatgtaac actttgacaa
     1081 tgcacaacgt gagttacgtg cgaggtctcc cctgcagtgt gggatttacg ctgattcagg
     1141 aatgggttgt tccctgggat atggttctga cgcgggagga gcttgtaatc ctgaggaagt
     1201 gtatgcacgt gtgcctgtgt tgtgccaaca ttgatatcat gacgagcatg atgatccatg
     1261 gttacgagtc ctgggctctc cactgtcatt gttccagtcc cggttccctg cagtgcatag
     1321 ccggcgggca ggttttggcc agctggttta ggatggtggt ggatggcgcc atgtttaatc
     1381 agaggtttat atggtaccgg gaggtggtga attacaacat gccaaaagag gtaatgttta
     1441 tgtccagcgt gtttatgagg ggtcgccact taatctacct gcgcttgtgg tatgatggcc
     1501 acgtgggttc tgtggtcccc gccatgagct ttggatacag cgccttgcac tgtgggattt
     1561 tgaacaatat tgtggtgctg tgctgcagtt actgtgctga tttaagtgag atcagggtgc
     1621 gctgctgtgc ccggaggaca aggcggctca tgctgcgggc ggtgcgaatc atcgctgagg
     1681 agaccactgc catgttgtat tcctgcagga cggagcggcg gcggcagcag tttattcgcg
     1741 cgctgctgca gcaccaccgc cctatcctga tgcacgatta tgactctacc cccatgtaga
     1801 tgccatttat ctattttact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
     1861 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
     1921 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
     1981 acagcaaggg ggaggattgg gaacacaata gcaggcatgc tggggatgcg gtgggctcta
     2041 tggccatcgt ctcaccatgg tctcaccatt cctgtagact tcttaattaa gacgtcagaa
     2101 ttctcgaggc ggccgcatgt gagtctccct atagtgagtc gtattaattt cgcgggcgga
     2161 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagtagcacc
     2221 aggcgtttaa gggcacccgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     2281 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     2341 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     2401 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     2461 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     2521 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     2581 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     2641 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     2701 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     2761 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     2821 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     2881 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     2941 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     3001 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtggat
     3061 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     3121 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     3181 cgtcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     3241 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatcctttac gtgccgatca
     3301 aagtctcatt ttcgccaaaa gttgtcatga ccaaaatccc ttaacgtgag ttttcgttcc
     3361 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     3421 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     3481 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     3541 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     3601 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     3661 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     3721 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     3781 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     3841 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     3901 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     3961 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa tgcggccttt ttacggttcc
     4021 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     4081 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     4141 gcagcgagtc agtgagcgag gaagc
//
