LOCUS       sequence-455002-       10532 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Adenoviral helper plasmid containing E2A, VA RNA I, E4orf6 and 
            L4-22k genes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10532)
  AUTHORS   Doshi J, Couto E, Staiti J, Vandenberghe LH, Zabaleta N
  TITLE     E2A, VA RNA I, and L4-22k adenoviral helper genes are sufficient for
            AAV production in HEK293 cells.
  JOURNAL   Mol Ther Methods Clin Dev. 2024 Nov 13;32(4):101376. doi: 
            10.1016/j.omtm.2024.101376. eCollection 2024 Dec 12.
  PUBMED    39670178
REFERENCE   2  (bases 1 to 10532)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther 
            Methods Clin Dev."; date: "2024-11-13"; volume: "32(4):101376. doi";
            pages: " 10.1016/j.omtm.2024.101376. eCollection 2024 Dec 12"
COMMENT     Sequence Label: JD150
FEATURES             Location/Qualifiers
     source          1..10532
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    52..123
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(128..174)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    192..240
                     /note="synthetic polyadenylation signal"
     promoter        277..1455
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          508..1446
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1403..1423
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1661..1670
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    2602..2723
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2613..2632)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2667..2686
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     polyA_signal    2732..2956
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        complement(4712..4915)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        4916..5219
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        complement(6905..7108)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        7109..7412
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     terminator      7535..7581
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      7611..7747
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    7792..7863
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     rep_origin      complement(8089..8677)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8169..8188)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(9423..9491,9492..10283)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9641..9660)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      10296..10432
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
ORIGIN
        1 tctcgtcagc tatcgtcagc ttaccttttt ggcagcgatc gcggctcccg acatcttgga
       61 ccattagctc cacaggtatc ttcttccctc tagtggtcat aacagcagct tcagctacct
      121 ctcaattcaa aaaacccctc aagacccgtt tagaggcccc aaggggttat gctatcaatc
      181 gttgcgttac acacacaaaa aaccaacaca catccatctt cgatggatag cgattttatt
      241 atctaactgc tgatcgagtg tagccagatc tcgtgaggct ccggtgcccg tcagtgggca
      301 gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaaccggt
      361 gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt
      421 tttcccgagg gtgggggaga accgtatata agtgcactag tcgccgtgaa cgttcttttt
      481 cgcaacgggt ttgccgccag aacacaggta agtgccgtgt gtggttcccg cgggcctggc
      541 ctctttacgg gttatggccc ttgcgtgcct tgaattactt ccacctggct gcagtacgtg
      601 attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgtggcc ttgcgcttaa
      661 ggagcccctt cgcctcgtgc ttgagttgtg gcctggcctg ggcgctgggg ccgccgcgtg
      721 cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa
      781 aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc
      841 caagatcagc acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg
      901 tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg
      961 gggtagtctc aagctgcccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc
     1021 ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg
     1081 cttcccggcc ctgctgcagg gagcacaaaa tggaggacgc ggcgctcggg agagcgggcg
     1141 ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac
     1201 tccacggagt accgggcgcc gtccaggcac ctcgattagt tctccagctt ttggagtacg
     1261 tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg
     1321 gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt
     1381 gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca
     1441 tttcaggtgt cgtgaaaact acccctaaaa gccaaaagat ctttgtcgat cctaccatcc
     1501 actcgacaca cccgccagcg gccgctgcca agcttccgag ctctcgaatt catcttggct
     1561 gaggaatctt ctaacaattt agagcttaaa aacgcccacg aggcggagaa cgaaatatcc
     1621 agagagacgt tagaaacgtt caaaaacgtt caccggtagg gccaccatgg cacccaaaaa
     1681 gaagctgcag ctgccgccgc cacccacgga cgaggaggaa tactgggaca gtcaggcaga
     1741 ggaggttttg gacgaggagg aggaggacat gatggaagac tgggagagcc tagacgagga
     1801 agcttccgag gtcgaagagg tgtcagacga aacaccgtca ccctcggtcg cattcccctc
     1861 gccggcgccc cagaaatcgg caaccggttc cagcatggct acaacctccg ctcctcaggc
     1921 gccgccggca ctgcccgttc gccgacccaa ccgtagatgg gacaccactg gaaccagggc
     1981 cggtaagtcc aagcagccgc cgccgttagc ccaagagcaa caacagcgcc aaggctaccg
     2041 ctcatggcgc gggcacaaga acgccatagt tgcttgcttg caagactgtg ggggcaacat
     2101 ctccttcgcc cgccgctttc ttctctacca tcacggcgtg gccttccccc gtaacatcct
     2161 gcattactac cgtcatctct acagcccata ctgcaccggc ggcagcggca gcaacagcag
     2221 cggccacaca gaagcaaagg cgaccggata gcaagactct gacaaagccc aagaaatcca
     2281 cagcggcggc agcagcagga ggaggagcgc tgcgtctggc gcccaacgaa cccgtatcga
     2341 cccgcgagct tagaaacagg atttttccca ctctgtatgc tatatttcaa cagagcaggg
     2401 gccaagaaca agagctgaaa ataaaaaaca ggtctctgcg atccctcacc cgcagctgcc
     2461 tgtatcacaa aagcgaagat cagcttcggc gcacgctgga agacgcggag gctctcttca
     2521 gtaaatactg cgcgctgact cttaaggact aggctagctt gactgactga gatacagcgt
     2581 accttcagct cacagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
     2641 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     2701 ttataagctg caataaacaa gttaggaatg gccatagagc ccaccgcatc cccagcatgc
     2761 ctgctattgt gttcccaatc ctcccccttg ctgtcctgcc ccaccccacc ccccagaata
     2821 gaatgacacc tactcagaca atgcgatgca atttcctcat tttattagga aaggacagtg
     2881 ggagtggcac cttccagggt caaggaaggc acgggggagg ggcaaacaac agatggctgg
     2941 caactagaag gcacagtaaa atagataaat ggcatcttgc cgtctgcgcc gtttaaaaat
     3001 caaaggggtt ctgccgcgca tcgctatgcg ccactggcag ggacacgttg cgatactggt
     3061 gtttagtgct ccacttaaac tcaggcacaa ccatccgcgg cagctcggtg aagttttcac
     3121 tccacaggct gcgcaccatc accaacgcgt ttagcaggtc gggcgccgat atcttgaagt
     3181 cgcagttggg gcctccgccc tgcgcgcgcg agttgcgata cacagggttg cagcactgga
     3241 acactatcag cgccgggtgg tgcacgctgg ccagcacgct cttgtcggag atcagatccg
     3301 cgtccaggtc ctccgcgttg ctcagggcga acggagtcaa ctttggtagc tgccttccca
     3361 aaaagggtgc atgcccaggc tttgagttgc actcgcaccg tagtggcatc agaaggtgac
     3421 cgtgcccggt ctgggcgtta ggatacagcg cctgcatgaa agccttgatc tgcttaaaag
     3481 ccacctgagc ctttgcgcct tcagagaaga acatgccgca agacttgccg gaaaactgat
     3541 tggccggaca ggccgcgtca tgcacgcagc accttgcgtc ggtgttggag atctgcacca
     3601 catttcggcc ccaccggttc ttcacgatct tggccttgct agactgctcc ttcagcgcgc
     3661 gctgcccgtt ttcgctcgtc acatccattt caatcacgtg ctccttattt atcataatgc
     3721 tcccgtgtag acacttaagc tcgccttcga tctcagcgca gcggtgcagc cacaacgcgc
     3781 agcccgtggg ctcgtggtgc ttgtaggtta cctctgcaaa cgactgcagg tacgcctgca
     3841 ggaatcgccc catcatcgtc acaaaggtct tgttgctggt gaaggtcagc tgcaacccgc
     3901 ggtgctcctc gtttagccag gtcttgcata cggccgccag agcttccact tggtcaggca
     3961 gtagcttgaa gtttgccttt agatcgttat ccacgtggta cttgtccatc aacgcgcgcg
     4021 cagcctccat gcccttctcc cacgcagaca cgatcggcag gctcagcggg tttatcaccg
     4081 tgctttcact ttccgcttca ctggactctt ccttttcctc ttgcgtccgc ataccccgcg
     4141 ccactgggtc gtcctcattc agccgccgca ccgtgcgctt acctcccttg ccgtgcttga
     4201 ttagcaccgg tgggttgctg aaacccacca tttgtagcgc cacatcttct ctttcttcct
     4261 cgctgtccac gatcacctct ggggatggcg ggcgctcggg cttgggagag gggcgcttct
     4321 ttttcttttt ggacgcaatg gccaaatccg ccgtcgaggt cgatggccgc gggctgggtg
     4381 tgcgcggcac cagcgcatct tgtgacgagt cctcttcgtc ctcggactcg agccgccgcc
     4441 tcagccgctt ttttgggggc gcgcggggag gcggcggcga cggcgacggg gacgacacgt
     4501 cctccatggt tggtggacgt cgcgccgcac cgcgtccgcg ctcgggggtg gtttcgcgct
     4561 gctcctcttc ccgactggcc atgctggcgg taccttcctt ctcctgatat cgcctcgctc
     4621 gacgaagtgc caaaaatctt tgagggtctt ggacgcgacg agaaacgcgc ggcaaacgct
     4681 ccgcagctgc ctgtatcaca acctaggtct gagctctgct tatatagacc tcccaccgta
     4741 cacgcctacc gcccatttgc gtcaatgggg cggagttgtt acgacatttt ggaaagtccc
     4801 gttgattttg gtgccaaaac aaactcccat tgacgtcaat ggggtggaga cttggaaatc
     4861 cccgtgagtc aaaccgctat ccacgcccat tgatgtactg ccaaaaccgc atcaccatgg
     4921 taatagcgat gactaatacg tagatgtact gccaagtagg aaagtcccat aaggtcatgt
     4981 actgggcata atgccaggcg ggccatttac cgtcattgac gtcaataggg ggcgtacttg
     5041 gcatatgata cacttgatgt actgccaagt gggcagttta ccgtaaatac tccacccatt
     5101 gacgtcaatg gaaagtccct attggcgtta ctatgggaac atacgtcatt attgacgtca
     5161 atgggcgggg gtcgttgggc ggtcagccag gcgggccatt taccgtaagt tatgtaacgc
     5221 tccgtaccag ctccatggtc gggacgctct ggccggtgag gcgtgcgcag tcgttgacgc
     5281 tctagaccgt gcaaaaggag agcctgtaag cgggcactct tccgtggtct ggtggataaa
     5341 ttcgcaaggg tatcatggcg gacgaccggg gttcgaaccc cggatccggc cgtccgccgt
     5401 gatccatgcg gttaccgccc gcgtgtcgaa cccaggtgtg cgacgtcaga caacggggga
     5461 gcgctccttt tggcttcctt ccaggcgcgg cggctgctgc gctagctttt ttggccactg
     5521 gccgcgcgcg gcgtaagcgg ttaggctgag aatcgtttgt gttatgtttc aacgtgttta
     5581 tttttcaatt gcagaaaatt tcaagtcatt tttcattcag tagtatagcc ccaccaccac
     5641 atagcttata cagatcaccg taccttaatc aaactcacag aaccctagta ttcaacctgc
     5701 cacctccctc ccaacacaca gagtacacag tcctttctcc ccggctggcc ttaaaaagca
     5761 tcatatcatg ggtaacagac atattcttag gtgttatatt ccacacggtt tcctgtcgag
     5821 ccaaacgctc atcagtgata ttaataaact ccccgggcag ctcacttaag ttcatgtcgc
     5881 tgtccagctg ctgagccaca ggctgctgtc caacttgcgg ttgcttaacg ggcggcgaag
     5941 gagaagtcca cgcctacatg ggggtagagt cataatcgtg catcaggata gggcggtggt
     6001 gctgcagcag cgcgcgaata aactgctgcc gccgccgctc cgtcctgcag gaatacaaca
     6061 tggcagtggt ctcctcagcg atgattcgca ccgcccgcag catgagccgc cttgtcctcc
     6121 gggcacagca gcgcaccctg atctcactta aatcagcaca gtaactgcag cacagcacca
     6181 caatattgtt caaaatccca cagtgcaagg cgctgtatcc aaagctcatg gcggggacca
     6241 cagaacccac gtggccatca taccacaagc gcaggtagat taagtggcga cccctcataa
     6301 acacgctgga cataaacatt acctcttttg gcatgttgta attcaccacc tcccggtacc
     6361 atataaacct ctgattaaac atggcgccat ccaccaccat cctaaaccag ctggccaaaa
     6421 cctgcccgcc ggctatgcac tgcagggaac cgggactgga acaatgacag tggagagccc
     6481 aggactcgta accatggatc atcatgctcg tcatgatatc aatgttggca caacacaggc
     6541 acacgtgcat acacttcctc aggattacaa gctcctcccg cgtcagaacc atatcccagg
     6601 gaacaaccca ttcctgaatc agcgtaaatc ccacactgca ggggagacct cgcacgtaac
     6661 tcacgttgtg cattgtcaaa gtgttacatt cgggcagcag cggatgatcc tccagtatgg
     6721 tagcgcgggt ctctgtctca aaaggaggta ggcgatccct actgtacgga gtgcgccgag
     6781 acaaccgaga tcgtgttggt cgtagtgtca tgccaaatgg aacgccggac gtagtcatat
     6841 ttccagtaaa aaaacctatt aaaaaacacc actcgacacg gcaccagctc aatcagtcac
     6901 agtgagctct gcttatatag acctcccacc gtacacgcct accgcccatt tgcgtcaatg
     6961 gggcggagtt gttacgacat tttggaaagt cccgttgatt ttggtgccaa aacaaactcc
     7021 cattgacgtc aatggggtgg agacttggaa atccccgtga gtcaaaccgc tatccacgcc
     7081 cattgatgta ctgccaaaac cgcatcacca tggtaatagc gatgactaat acgtagatgt
     7141 actgccaagt aggaaagtcc cataaggtca tgtactgggc ataatgccag gcgggccatt
     7201 taccgtcatt gacgtcaata gggggcgtac ttggcatatg atacacttga tgtactgcca
     7261 agtgggcagt ttaccgtaaa tactccaccc attgacgtca atggaaagtc cctattggcg
     7321 ttactatggg aacatacgtc attattgacg tcaatgggcg ggggtcgttg ggcggtcagc
     7381 caggcgggcc atttaccgta agttatgtaa cgctcccgtc aacaacaaca attgcattca
     7441 ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta
     7501 caaatgtggt attggcccat ctctatcggt atcgtagcat aaccccttgg ggcctctaaa
     7561 cgggtcttga ggggtttttt gtgcccctcg ggccggattg ctatctaccg gcattggcgc
     7621 agaaaaaaat gcctgatgcg acgctgcgcg tcttatactc ccacatatgc cagattcagc
     7681 aacggatacg gcttccccaa cttgcccact tccatacgtg tcctccttac cagaaattta
     7741 tccttaaggt cgtcagctat cctgcaggcg atctctcgat ttcgatcaag acattccttt
     7801 aatggtcttt tctggacacc actaggggtc agaagtagtt catcaaactt tcttccctcc
     7861 ctaatctcat tggttacctt gggctatcga aacttaatta accagtcaag tcagctactt
     7921 ggcgagatcg acttgtctgg gtttcgacta cgctcagaat tgcgtcagtc aagttcgatc
     7981 tggtccttgc tattgcaccc gttctccgat tacgagtttc atttaaatca tgtgagcaaa
     8041 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     8101 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     8161 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     8221 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     8281 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     8341 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     8401 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     8461 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     8521 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     8581 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     8641 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     8701 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     8761 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     8821 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     8881 agcgatctgt ctatttcgtt catccatagt tgcatttaaa tttccgaact ctccaaggcc
     8941 ctcgtcggaa aatcttcaaa cctttcgtcc gatccatctt gcaggctacc tctcgaacga
     9001 actatcgcaa gtctcttggc cggccttgcg ccttggctat tgcttggcag cgcctatcgc
     9061 caggtattac tccaatcccg aatatccgag atcgggatca cccgagagaa gttcaaccta
     9121 catcctcaat cccgatctat ccgagatccg aggaatatcg aaatcggggc gcgcctggtg
     9181 taccgagaac gatcctctca gtgcgagtct cgacgatcca tatcgttgct tggcagtcag
     9241 ccagtcggaa tccagcttgg gacccaggaa gtccaatcgt cagatattgt actcaagcct
     9301 ggtcacggca gcgtaccgat ctgtttaaac ctagatattg atagtctgat cggtcaacgt
     9361 ataatcgagt cctagctttt gcaaacatct atcaagagac aggatcagca ggaggctttc
     9421 gcatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     9481 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     9541 cgcgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     9601 ccgaagaacg ctttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     9661 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     9721 tggttgagta ttcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     9781 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     9841 ttggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     9901 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     9961 tgcctgtagc aatggcaaca accttgcgta aactattaac tggcgaacta cttactctag
    10021 cttcccggca acagttgata gactggatgg aggcggataa agttgcagga ccacttctgc
    10081 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
    10141 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
    10201 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
    10261 cctcactgat taagcattgg taaccgattc taggtgcatt ggcgcagaaa aaaatgcctg
    10321 atgcgacgct gcgcgtctta tactcccaca tatgccagat tcagcaacgg atacggcttc
    10381 cccaacttgc ccacttccat acgtgtcctc cttaccagaa atttatcctt aagatcccga
    10441 atcgtttaaa ctcgactctg gctctatcga atctccgtcg tttcgagctt acgcgaacag
    10501 ccgtggcgct catttgctcg tcgggcatcg aa
//
