LOCUS       sequence-455003-        4906 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CMV promoter driving expression of adenoviral E2A gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4906)
  AUTHORS   Doshi J, Couto E, Staiti J, Vandenberghe LH, Zabaleta N
  TITLE     E2A, VA RNA I, and L4-22k adenoviral helper genes are sufficient for
            AAV production in HEK293 cells.
  JOURNAL   Mol Ther Methods Clin Dev. 2024 Nov 13;32(4):101376. doi: 
            10.1016/j.omtm.2024.101376. eCollection 2024 Dec 12.
  PUBMED    39670178
REFERENCE   2  (bases 1 to 4906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther 
            Methods Clin Dev."; date: "2024-11-13"; volume: "32(4):101376. doi";
            pages: " 10.1016/j.omtm.2024.101376. eCollection 2024 Dec 12"
COMMENT     Sequence Label: JD94
FEATURES             Location/Qualifiers
     source          1..4906
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        254..272
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     254..271
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     enhancer        319..622
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        623..826
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     777..797
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    2582..2806
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(2868..2887)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2869..2887)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        2979..3083
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3084..3152,3153..3944)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3302..3321)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4142..4730
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4631..4650
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4884..4901
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgaaaaatca ataatcagac aacaagatgt gcgaactcga tattttacac gactctcttt
       61 accaattctg ccccgaatta cacttaaaac gactcaacag cttaacgttg gcttgccacg
      121 cattacttga ctgtaaaact ctcactctta ccgaacttgg ccgtaacctg ccaaccaaag
      181 cgagaacaaa acataacatc aaacgaatcg accgattgtt aggtaatcgt cacctgcagg
      241 aaggtttaaa cgcatttagg tgacactata gaagtgtgta tcgctcgagg gatccgaatt
      301 cgaagtcttg gtacggagcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
      361 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
      421 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
      481 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
      541 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
      601 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
      661 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
      721 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
      781 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcaga cctaggttgt
      841 gatacaggca gctgcggagc gtttgccgcg cgtttctcgt cgcgtccaag accctcaaag
      901 atttttggca cttcgtcgag cgaggcgata tcaggagaag gaaggtaccg ccagcatggc
      961 cagtcgggaa gaggagcagc gcgaaaccac ccccgagcgc ggacgcggtg cggcgcgacg
     1021 tccaccaacc atggaggacg tgtcgtcccc gtcgccgtcg ccgccgcctc cccgcgcgcc
     1081 cccaaaaaag cggctgaggc ggcggctcga gtccgaggac gaagaggact cgtcacaaga
     1141 tgcgctggtg ccgcgcacac ccagcccgcg gccatcgacc tcgacggcgg atttggccat
     1201 tgcgtccaaa aagaaaaaga agcgcccctc tcccaagccc gagcgcccgc catccccaga
     1261 ggtgatcgtg gacagcgagg aagaaagaga agatgtggcg ctacaaatgg tgggtttcag
     1321 caacccaccg gtgctaatca agcacggcaa gggaggtaag cgcacggtgc ggcggctgaa
     1381 tgaggacgac ccagtggcgc ggggtatgcg gacgcaagag gaaaaggaag agtccagtga
     1441 agcggaaagt gaaagcacgg tgataaaccc gctgagcctg ccgatcgtgt ctgcgtggga
     1501 gaagggcatg gaggctgcgc gcgcgttgat ggacaagtac cacgtggata acgatctaaa
     1561 ggcaaacttc aagctactgc ctgaccaagt ggaagctctg gcggccgtat gcaagacctg
     1621 gctaaacgag gagcaccgcg ggttgcagct gaccttcacc agcaacaaga cctttgtgac
     1681 gatgatgggg cgattcctgc aggcgtacct gcagtcgttt gcagaggtaa cctacaagca
     1741 ccacgagccc acgggctgcg cgttgtggct gcaccgctgc gctgagatcg aaggcgagct
     1801 taagtgtcta cacgggagca ttatgataaa taaggagcac gtgattgaaa tggatgtgac
     1861 gagcgaaaac gggcagcgcg cgctgaagga gcagtctagc aaggccaaga tcgtgaagaa
     1921 ccggtggggc cgaaatgtgg tgcagatctc caacaccgac gcaaggtgct gcgtgcatga
     1981 cgcggcctgt ccggccaatc agttttccgg caagtcttgc ggcatgttct tctctgaagg
     2041 cgcaaaggct caggtggctt ttaagcagat caaggctttc atgcaggcgc tgtatcctaa
     2101 cgcccagacc gggcacggtc accttctgat gccactacgg tgcgagtgca actcaaagcc
     2161 tgggcatgca ccctttttgg gaaggcagct accaaagttg actccgttcg ccctgagcaa
     2221 cgcggaggac ctggacgcgg atctgatctc cgacaagagc gtgctggcca gcgtgcacca
     2281 cccggcgctg atagtgttcc agtgctgcaa ccctgtgtat cgcaactcgc gcgcgcaggg
     2341 cggaggcccc aactgcgact tcaagatatc ggcgcccgac ctgctaaacg cgttggtgat
     2401 ggtgcgcagc ctgtggagtg aaaacttcac cgagctgccg cggatggttg tgcctgagtt
     2461 taagtggagc actaaacacc agtatcgcaa cgtgtccctg ccagtggcgc atagcgatgc
     2521 gcggcagaac ccctttgatt tttaaacggc gcagacggca agatgccatt tatctatttt
     2581 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     2641 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     2701 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     2761 tgggaacaca atagcaggca tgctggggat gcggtgggct ctatggccat tcctgtagac
     2821 ttcttaatta agacgtcaga attctcgagg cggccgcatg tgagtctccc tatagtgagt
     2881 cgtattaatt tcgcgggcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     2941 tatccgctca tgagtagcac caggcgttta agggcacccg cggaacccct atttgtttat
     3001 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     3061 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     3121 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     3181 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     3241 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     3301 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     3361 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     3421 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     3481 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     3541 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     3601 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     3661 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     3721 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     3781 ctggagccgg tgagcgtgga tctcgcggta tcattgcagc actggggcca gatggtaagc
     3841 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     3901 gacagatcgc tgagataggt gcgtcactga ttaagcattg gtaactgtca gaccaagttt
     3961 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     4021 agatccttta cgtgccgatc aaagtctcat tttcgccaaa agttgtcatg accaaaatcc
     4081 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     4141 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     4201 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     4261 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     4321 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     4381 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     4441 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     4501 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     4561 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     4621 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     4681 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     4741 atgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     4801 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     4861 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagc
//
