LOCUS       sequence-455010-        2656 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Adenoviral VA RNA II gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Doshi J, Couto E, Staiti J, Vandenberghe LH, Zabaleta N
  TITLE     E2A, VA RNA I, and L4-22k adenoviral helper genes are sufficient for
            AAV production in HEK293 cells.
  JOURNAL   Mol Ther Methods Clin Dev. 2024 Nov 13;32(4):101376. doi: 
            10.1016/j.omtm.2024.101376. eCollection 2024 Dec 12.
  PUBMED    39670178
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther 
            Methods Clin Dev."; date: "2024-11-13"; volume: "32(4):101376. doi";
            pages: " 10.1016/j.omtm.2024.101376. eCollection 2024 Dec 12"
COMMENT     Sequence Label: JD81
FEATURES             Location/Qualifiers
     source          1..2656
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        254..272
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     254..271
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(618..637)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(619..637)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        729..833
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(834..902,903..1694)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1052..1071)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1892..2480
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2381..2400
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2634..2651
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgaaaaatca ataatcagac aacaagatgt gcgaactcga tattttacac gactctcttt
       61 accaattctg ccccgaatta cacttaaaac gactcaacag cttaacgttg gcttgccacg
      121 cattacttga ctgtaaaact ctcactctta ccgaacttgg ccgtaacctg ccaaccaaag
      181 cgagaacaaa acataacatc aaacgaatcg accgattgtt aggtaatcgt cacctgcagg
      241 aaggtttaaa cgcatttagg tgacactata gaagtgtgta tcgctcgagg gatccgaatt
      301 cgaagtcttg gtacggaggt taggctggaa agcgaaagca ttaagtggct cgctccctgt
      361 agccggaggg ttattttcca agggttgagt cgcaggaccc ccggttcgag tctcgggccg
      421 gccggactgc ggcgaacggg ggtttgcctc cccgtcatgc aagaccccgc ttgcaaattc
      481 ctccggaaac agggacgagc cccttttttg cttttcccag atgcatccgg tgctgcggca
      541 gatgcgccat tcctgtccat tcctgtagac ttcttaatta agacgtcaga attctcgagg
      601 cggccgcatg tgagtctccc tatagtgagt cgtattaatt tcgcgggcgg aacccctatt
      661 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagtagcac caggcgttta
      721 agggcacccg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
      781 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
      841 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
      901 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
      961 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     1021 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     1081 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     1141 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     1201 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     1261 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     1321 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     1381 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     1441 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     1501 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtgga tctcgcggta
     1561 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     1621 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcgtcactga
     1681 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     1741 ttcattttta atttaaaagg atctaggtga agatccttta cgtgccgatc aaagtctcat
     1801 tttcgccaaa agttgtcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1861 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1921 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1981 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     2041 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     2101 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     2161 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     2221 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     2281 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     2341 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     2401 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     2461 gggggcggag cctatggaaa aacgccagca atgcggcctt tttacggttc ctggcctttt
     2521 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     2581 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     2641 cagtgagcga ggaagc
//
