LOCUS       sequence-455015-        4287 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CMV promoter driving expression of adenoviral E4orf6-6/7 gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4287)
  AUTHORS   Doshi J, Couto E, Staiti J, Vandenberghe LH, Zabaleta N
  TITLE     E2A, VA RNA I, and L4-22k adenoviral helper genes are sufficient for
            AAV production in HEK293 cells.
  JOURNAL   Mol Ther Methods Clin Dev. 2024 Nov 13;32(4):101376. doi: 
            10.1016/j.omtm.2024.101376. eCollection 2024 Dec 12.
  PUBMED    39670178
REFERENCE   2  (bases 1 to 4287)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther 
            Methods Clin Dev."; date: "2024-11-13"; volume: "32(4):101376. doi";
            pages: " 10.1016/j.omtm.2024.101376. eCollection 2024 Dec 12"
COMMENT     Sequence Label: JD143
FEATURES             Location/Qualifiers
     source          1..4287
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        254..272
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     254..271
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     enhancer        341..644
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        645..848
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     799..819
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     complement(2249..2268)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2250..2268)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        2360..2464
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2465..2533,2534..3325)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2683..2702)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3523..4111
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4012..4031
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4265..4282
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgaaaaatca ataatcagac aacaagatgt gcgaactcga tattttacac gactctcttt
       61 accaattctg ccccgaatta cacttaaaac gactcaacag cttaacgttg gcttgccacg
      121 cattacttga ctgtaaaact ctcactctta ccgaacttgg ccgtaacctg ccaaccaaag
      181 cgagaacaaa acataacatc aaacgaatcg accgattgtt aggtaatcgt cacctgcagg
      241 aaggtttaaa cgcatttagg tgacactata gaagtgtgta tcgctcgagg gatccgaatt
      301 cgaagtcttg gtacggagcg agaccggagc gagacgggag cgttacataa cttacggtaa
      361 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
      421 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
      481 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
      541 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
      601 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
      661 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
      721 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
      781 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
      841 gcagagctca ctgtgactga ttgagctggt gccgtgtcga gtggtgtttt ttaataggtt
      901 tttttactgg aaatatgact acgtccggcg ttccatttgg catgacacta cgaccaacac
      961 gatctcggtt gtctcggcgc actccgtaca gtagggatcg cctacctcct tttgagacag
     1021 agacccgcgc taccatactg gaggatcatc cgctgctgcc cgaatgtaac actttgacaa
     1081 tgcacaacgt gagttacgtg cgaggtctcc cctgcagtgt gggatttacg ctgattcagg
     1141 aatgggttgt tccctgggat atggttctga cgcgggagga gcttgtaatc ctgaggaagt
     1201 gtatgcacgt gtgcctgtgt tgtgccaaca ttgatatcat gacgagcatg atgatccatg
     1261 gttacgagtc ctgggctctc cactgtcatt gttccagtcc cggttccctg cagtgcatag
     1321 ccggcgggca ggttttggcc agctggttta ggatggtggt ggatggcgcc atgtttaatc
     1381 agaggtttat atggtaccgg gaggtggtga attacaacat gccaaaagag gtaatgttta
     1441 tgtccagcgt gtttatgagg ggtcgccact taatctacct gcgcttgtgg tatgatggcc
     1501 acgtgggttc tgtggtcccc gccatgagct ttggatacag cgccttgcac tgtgggattt
     1561 tgaacaatat tgtggtgctg tgctgcagtt actgtgctga tttaagtgag atcagggtgc
     1621 gctgctgtgc ccggaggaca aggcggctca tgctgcgggc ggtgcgaatc atcgctgagg
     1681 agaccactgc catgttgtat tcctgcagga cggagcggcg gcggcagcag tttattcgcg
     1741 cgctgctgca gcaccaccgc cctatcctga tgcacgatta tgactctacc cccatgtagg
     1801 cgtggacttc tccttcgccg cccgttaagc aaccgcaagt tggacagcag cctgtggctc
     1861 agcagctgga cagcgacatg aacttaagtg agctgcccgg ggagtttatt aatatcactg
     1921 atgagcgttt ggctcgacag gaaaccgtgt ggaatataac acctaagaat atgtctgtta
     1981 cccatgatat gatgcttttt aaggccagcc ggggagaaag gactgtgtac tctgtgtgtt
     2041 gggagggagg tggcaggttg aatactaggg ttctgtgagt ttgattaagg tacggtgatc
     2101 tgtataagct atgtggtggt ggggctatac tactgaatga aaaatgactt gaaattttct
     2161 gcaattgaaa aataaacacg ttgaaacata acacaaacga ttctttaatt aagacgtcag
     2221 aattctcgag gcggccgcat gtgagtctcc ctatagtgag tcgtattaat ttcgcgggcg
     2281 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagtagca
     2341 ccaggcgttt aagggcaccc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     2401 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     2461 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     2521 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     2581 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     2641 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     2701 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     2761 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     2821 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     2881 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     2941 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     3001 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     3061 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     3121 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     3181 atctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     3241 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     3301 tgcgtcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     3361 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt acgtgccgat
     3421 caaagtctca ttttcgccaa aagttgtcat gaccaaaatc ccttaacgtg agttttcgtt
     3481 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     3541 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     3601 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     3661 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     3721 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     3781 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     3841 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     3901 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     3961 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     4021 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     4081 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aatgcggcct ttttacggtt
     4141 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     4201 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     4261 gcgcagcgag tcagtgagcg aggaagc
//
