LOCUS       sequence-455018-        5396 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Adenoviral E4 gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5396)
  AUTHORS   Doshi J, Couto E, Staiti J, Vandenberghe LH, Zabaleta N
  TITLE     E2A, VA RNA I, and L4-22k adenoviral helper genes are sufficient for
            AAV production in HEK293 cells.
  JOURNAL   Mol Ther Methods Clin Dev. 2024 Nov 13;32(4):101376. doi: 
            10.1016/j.omtm.2024.101376. eCollection 2024 Dec 12.
  PUBMED    39670178
REFERENCE   2  (bases 1 to 5396)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther 
            Methods Clin Dev."; date: "2024-11-13"; volume: "32(4):101376. doi";
            pages: " 10.1016/j.omtm.2024.101376. eCollection 2024 Dec 12"
COMMENT     Sequence Label: JD134
FEATURES             Location/Qualifiers
     source          1..5396
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        254..272
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     254..271
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3358..3377)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3359..3377)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        3469..3573
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3574..3642,3643..4434)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3792..3811)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4632..5220
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5121..5140
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5374..5391
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgaaaaatca ataatcagac aacaagatgt gcgaactcga tattttacac gactctcttt
       61 accaattctg ccccgaatta cacttaaaac gactcaacag cttaacgttg gcttgccacg
      121 cattacttga ctgtaaaact ctcactctta ccgaacttgg ccgtaacctg ccaaccaaag
      181 cgagaacaaa acataacatc aaacgaatcg accgattgtt aggtaatcgt cacctgcagg
      241 aaggtttaaa cgcatttagg tgacactata gaagtgtgta tcgctcgagg gatccaaatg
      301 ggaagttacg taacgtggga aaacggaagt gacgatttga ggaagttgtg ggttttttgg
      361 ctttcgtttc tgggcgtagg ttcgcgtgcg gttttctggg tgttttttgt ggactttaac
      421 cgttacgtca ttttttagtc ctatatatac tcgctctgca cttggccctt ttttacactg
      481 tgactgattg agctggtgcc gtgtcgagtg gtgttttttt aataggtttt cttttttact
      541 ggtaaggctg actgttatgg ctgccgctgt ggaagcgctg tatgttgttc tggagcggga
      601 gggtgctatt ttgcctaggc aggagggttt ttcaggtgtt tatgtgtttt tctctcctat
      661 taattttgtt atacctccta tgggggctgt aatgttgtct ctacgcctgc gggtatgtat
      721 tcccccgggc tatttcggtc gctttttagc actgaccgat gtgaatcaac ctgatgtgtt
      781 taccgagtct tacattatga ctccggacat gaccgaggag ctgtcggtgg tgctttttaa
      841 tcacggtgac cagttttttt acggtcacgc cggcatggcc gtagtccgtc ttatgcttat
      901 aagggttgtt tttcctgttg taagacaggc ttctaatgtt taaatgtttt tttgttattt
      961 tattttgtgt ttatgcagaa acccgcagac atgtttgaga gaaaaatggt gtctttttct
     1021 gtggtggttc cggagcttac ctgcctttat ctgcatgagc atgactacga tgtgctttct
     1081 tttttgcgcg aggctttgcc tgattttttg agcagcacct tgcattttat atcgccgccc
     1141 atgcaacaag cttacatcgg ggctacgctg gttagcatag ctccgagtat gcgtgtcata
     1201 atcagtgtgg gttcttttgt catggttcct ggcggggaag tggccgcgct ggtccgtgca
     1261 gacctgcacg attatgttca gctggccctg cgaagggacc tacgggatcg cggtattttt
     1321 gttaatgttc cgcttttgaa tcttatacag gtctgtgagg aacctgaatt tttgcaatca
     1381 tgattcgctg cttgaggctg aaggtggagg gcgctctgga gcagattttt acaatggccg
     1441 gacttaatat tcgggatttg cttagagata tattgagaag gtggcgagat gagaattatt
     1501 tgggcatggt tgaaggtgct ggaatgttta tagaggagat tcaccctgaa gggtttagcc
     1561 tttacgtcca cttggacgtg agggccgttt gccttttgga agccattgtg caacatctta
     1621 caaatgccat tatctgttct ttggctgtag agtttgacca cgccaccgga ggggagcgcg
     1681 ttcacttaat agatcttcat tttgaggttt tggataatct tttggaataa aaaaaaaaac
     1741 atggttcttc cagctcttcc cgctcctccc gtgtgtgact cgcagaacga atgtgtaggt
     1801 tggctgggtg tggcttattc tgcggtggtg gatgttatca gggcagcggc gcatgaagga
     1861 gtttacatag aacccgaagc cagggggcgc ctggatgctt tgagagagtg gatatactac
     1921 aactactaca cagagcgatc taagcggcga gaccggagac gcagatctgt ttgtcacgcc
     1981 cgcacctggt tttgcttcag gaaatatgac tacgtccggc gttccatttg gcatgacact
     2041 acgaccaaca cgatctcggt tgtctcggcg cactccgtac agtagggatc gtctacctcc
     2101 ttttgagaca gaaacccgcg ctaccatact ggaggatcat ccgctgctgc ccgaatgtaa
     2161 cactttgaca atgcacaacg tgagttacgt gcgaggtctt ccctgcagtg tgggatttac
     2221 gctgattcag gaatgggttg ttccctggga tatggttcta acgcgggagg agcttgtaat
     2281 cctgaggaag tgtatgcacg tgtgcctgtg ttgtgccaac attgatatca tgacgagcat
     2341 gatgatccat ggttacgagt cctgggctct ccactgtcat tgttccagtc ccggttccct
     2401 gcagtgtata gccggcgggc aggttttggc cagctggttt aggatggtgg tggatggcgc
     2461 catgtttaat cagaggttta tatggtaccg ggaggtggtg aattacaaca tgccaaaaga
     2521 ggtaatgttt atgtccagcg tgtttatgag gggtcgccac ttaatctacc tgcgcttgtg
     2581 gtatgatggc cacgtgggtt ctgtggtccc cgccatgagc tttggataca gcgccttgca
     2641 ctgtgggatt ttgaacaata ttgtggtgct gtgctgcagt tactgtgctg atttaagtga
     2701 gatcagggtg cgctgctgtg cccggaggac aaggcgcctt atgctgcggg cggtgcgaat
     2761 catcgctgag gagaccactg ccatgttgta ttcctgcagg acggagcggc ggcggcagca
     2821 gtttattcgc gcgctgctgc agcaccaccg ccctatcctg atgcacgatt atgactctac
     2881 ccccatgtag gcgtggactt ctccttcgcc gcccgttaag caaccgcaag ttggacagca
     2941 gcctgtggct cagcagctgg acagcgacat gaacttaagt gagctgcccg gggagtttat
     3001 taatatcact gatgagcgtt tggctcgaca ggaaaccgtg tggaatataa cacctaagaa
     3061 tatgtctgtt acccatgata tgatgctttt taaggccagc cggggagaaa ggactgtgta
     3121 ctctgtgtgt tgggagggag gtggcaggtt gaatactagg gttctgtgag tttgattaag
     3181 gtacggtgat ctgtataagc tatgtggtgg tggggctata ctactgaatg aaaaatgact
     3241 tgaaattttc tgcaattgaa aaataaacac gttgaaacat aacacaaacg gtctcaccat
     3301 tcctgtagac ttcttaatta agacgtcaga attctcgagg cggccgcatg tgagtctccc
     3361 tatagtgagt cgtattaatt tcgcgggcgg aacccctatt tgtttatttt tctaaataca
     3421 ttcaaatatg tatccgctca tgagtagcac caggcgttta agggcacccg cggaacccct
     3481 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     3541 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     3601 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     3661 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     3721 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     3781 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     3841 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     3901 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     3961 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     4021 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     4081 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     4141 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     4201 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     4261 gctgataaat ctggagccgg tgagcgtgga tctcgcggta tcattgcagc actggggcca
     4321 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     4381 gaacgaaata gacagatcgc tgagataggt gcgtcactga ttaagcattg gtaactgtca
     4441 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     4501 atctaggtga agatccttta cgtgccgatc aaagtctcat tttcgccaaa agttgtcatg
     4561 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     4621 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     4681 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     4741 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     4801 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     4861 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     4921 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     4981 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     5041 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     5101 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     5161 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     5221 aacgccagca atgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     5281 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     5341 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagc
//
