LOCUS       sequence-455019-       11681 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Stable expression of V-ATPase subunit ATP6V0A3 with N-terminal 
            3xFLAG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11681)
  AUTHORS   Ratto E, Chowdhury SR, Siefert NS, Schneider M, Wittmann M, Helm D, 
            Palm W
  TITLE     Direct control of lysosomal catabolic activity by mTORC1 through 
            regulation of V-ATPase assembly.
  JOURNAL   Nat Commun. 2022 Aug 17;13(1):4848. doi: 10.1038/s41467-022-32515-6.
  PUBMED    35977928
REFERENCE   2  (bases 1 to 11681)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-08-17"; volume: "13(1):4848. doi"; pages: " 
            10.1038/s41467-022-32515-6"
COMMENT     Sequence Label: pLV_3xFLAG-ATP6V0A3
FEATURES             Location/Qualifiers
     source          1..11681
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    109..658
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(253..270)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     480..499
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             661..1686
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             661..1686
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     misc_feature    1710..2298
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1763..1783)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2181..2192)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2505..3138
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3266..3282)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3266..3282)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3279..3301)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3290..3306
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3314..3320,3321..3338,3339..3344))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3359..3380
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3497..3514)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3668..4256)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3748..3767)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4427..5218,5219..5287))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5050..5069
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5288..5392)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    5440..5574
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5477..5496)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5531..5550
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5742..6375
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6422..6547
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7044..7277
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7462..7506
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7769..7884
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        7962..8173
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          8174..9112
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     9069..9089
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             9126..9191
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     regulatory      10623..10632
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cctcgagggc ggccgctcta gagtcgacgg gccgcggtaa caattgttaa ctaacttaag
       61 ctagcaacgg tttccctcta gcgggatcaa ttccgccccc cccccctaac gttactggcc
      121 gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc
      181 cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta
      241 ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag
      301 ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga
      361 accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg
      421 caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat
      481 ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta
      541 tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa
      601 aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgataatacc
      661 atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac
      721 agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat
      781 gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat
      841 cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt
      901 ggggaattta gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg
      961 caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga ggccatggat
     1021 gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga
     1081 atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat
     1141 cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag
     1201 ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc
     1261 tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg
     1321 atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct
     1381 tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg
     1441 cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac
     1501 ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga
     1561 gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc
     1621 tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc gagggcaaag
     1681 gaatagcccg ggcggggcgc gtctggaaca atcaacctct ggattacaaa atttgtgaaa
     1741 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     1801 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     1861 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     1921 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     1981 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     2041 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     2101 ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     2161 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     2221 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     2281 tttgggccgc ctccccgcct ggaattaatt ctgcagtcga gacctagaaa aacatggagc
     2341 aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga
     2401 ggaggaggag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa
     2461 ggcagctgta gatcttagcc actttttaaa agaaaagagg ggactggaag ggctaattca
     2521 ctcccaacga agacaagata tccttgatct gtggatctac cacacacaag gctacttccc
     2581 tgattagcag aactacacac cagggccagg ggtcagatat ccactgacct ttggatggtg
     2641 ctacaagcta gtaccagttg agccagataa ggtagaagag gccaataaag gagagaacac
     2701 cagcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag aagtgttaga
     2761 gtggaggttt gacagccgcc tagcatttca tcacgtggcc cgagagctgc atccggagta
     2821 cttcaagaac tgctgatatc gagcttgcta caagggactt tccgctgggg actttccagg
     2881 gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatcctgca tataagcagc
     2941 tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     3001 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     3061 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     3121 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg
     3181 caaagaaatg aatatcagag agtgagaggc cttgacattg ctagcgttta ccgtcgacct
     3241 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     3301 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     3361 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     3421 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     3481 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     3541 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     3601 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     3661 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     3721 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     3781 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     3841 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     3901 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     3961 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     4021 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     4081 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     4141 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     4201 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     4261 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     4321 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     4381 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     4441 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     4501 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     4561 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     4621 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     4681 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     4741 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     4801 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     4861 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     4921 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     4981 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     5041 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     5101 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     5161 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     5221 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     5281 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     5341 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     5401 cccgaaaagt gccacctgac gtcgacggat cgggagatca acttgtttat tgcagcttat
     5461 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     5521 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg
     5581 ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc
     5641 aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa
     5701 agaaattgta tttgttaaat atgtactaca aacttagtag ttggaagggc taattcactc
     5761 ccaaagaaga caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga
     5821 ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta
     5881 caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag
     5941 cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag tgttagagtg
     6001 gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt
     6061 caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag
     6121 gcgtggcctg ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc
     6181 tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     6241 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     6301 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     6361 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga
     6421 gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
     6481 actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
     6541 gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc
     6601 agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg
     6661 attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca
     6721 gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc
     6781 aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa
     6841 gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg gccgctgatc
     6901 ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa
     6961 gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag
     7021 agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga
     7081 agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt
     7141 atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa
     7201 ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta
     7261 aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct
     7321 gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc
     7381 tggatggagt gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa
     7441 gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca
     7501 agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg
     7561 atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga
     7621 gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc
     7681 gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag atccattcga
     7741 ttagtgaacg gatctcgacg gtatcgcctt taaaagaaaa ggggggattg gggggtacag
     7801 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
     7861 acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag atccagttta
     7921 tcgatgaggc cctttcgtct tcactcgagg tgcccgtcag tgggcagagc gcacatcgcc
     7981 cacagtcccc gagaagttgg ggggaggggt cggcaattga accggtgcct agagaaggtg
     8041 gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg
     8101 gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc
     8161 cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta
     8221 tggcccttgc gtgccttgaa ttacttccac ctggctgcag tacgtgattc ttgatcccga
     8281 gcttcgggtt ggaagtgggt gggagagttc gaggccttgc gcttaaggag ccccttcgcc
     8341 tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca
     8401 ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc atttaaaatt tttgatgacc
     8461 tgctgcgacg ctttttttct ggcaagatag tcttgtaaat gcgggccaag atctgcacac
     8521 tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg
     8581 ttcggcgagg cggggcctgc gagcgcggcc accgagaatc ggacgggggt agtctcaagc
     8641 tggccggcct gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc
     8701 aaggctggcc cggtcggcac cagttgcgtg agcggaaaga tggccgcttc ccggccctgc
     8761 tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg agtcacccac
     8821 acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg
     8881 ggcgccgtcc aggcacctcg attagttctc gagcttttgg agtacgtcgt ctttaggttg
     8941 gggggagggg ttttatgcga tggagtttcc ccacactgag tgggtggaga ctgaagttag
     9001 gccagcttgg cacttgatgt aattctcctt ggaatttgcc ctttttgagt ttggatcttg
     9061 gttcattctc aagcctcaga cagtggttca aagttttttt cttccatttc aggtgtcgtg
     9121 agatggacta caaagaccat gacggtgatt ataaagatca tgatatcgat tacaaggatg
     9181 acgatgacaa gggctccatg ttccggagcg aggaggtggc cctggtccag ctctttctgc
     9241 ccacagcggc tgcctacacc tgcgtgagtc ggctgggcga gctgggcctc gtggagttca
     9301 gagacctcaa cgcctcggtg agcgccttcc agagacgctt tgtggttgat gttcggcgct
     9361 gtgaggagct ggagaagacc ttcaccttcc tgcaggagga ggtgcggcgg gctgggctgg
     9421 tcctgccccc gccaaagggg aggctgccgg cacccccacc ccgggacctg ctgcgcatcc
     9481 aggaggagac ggagcgcctg gcccaggagc tgcgggatgt gcggggcaac cagcaggccc
     9541 tgcgggccca gctgcaccag ctgcagctcc acgccgccgt gctacgccag ggccatgaac
     9601 ctcagctggc agccgcccac acagatgggg cctcagagag gacgcccctg ctccaggccc
     9661 ccggggggcc gcaccaggac ctgagggtca actttgtggc aggtgccgtg gagccccaca
     9721 aggcccctgc cctagagcgc ctgctctgga gggcctgccg cggcttcctc attgccagct
     9781 tcagggagct ggagcagccg ctggagcacc ccgtgacggg cgagccagcc acgtggatga
     9841 ccttcctcat ctcctactgg ggtgagcaga tcggacagaa gatccgcaag atcacggact
     9901 gcttccactg ccacgtcttc ccgtttctgc agcaggagga ggcccgcctc ggggccctgc
     9961 agcagctgca acagcagagc caggagctgc aggaggtcct cggggagaca gagcggttcc
    10021 tgagccaggt gctaggccgg gtgctgcagc tgctgccgcc agggcaggtg caggtccaca
    10081 agatgaaggc cgtgtacctg gccctgaacc agtgcagcgt gagcaccacg cacaagtgcc
    10141 tcattgccga ggcctggtgc tctgtgcgag acctgcccgc cctgcaggag gccctgcggg
    10201 acagctcgat ggaggaggga gtgagtgccg tggctcaccg catcccctgc cgggacatgc
    10261 cccccacact catccgcacc aaccgcttca cggccagctt ccagggcatc gtggatgcct
    10321 acggcgtggg ccgctaccag gaggtcaacc ccgctcccta caccatcatc accttcccct
    10381 tcctgtttgc tgtgatgttc ggggatgtgg gccacgggct gctcatgttc ctcttcgccc
    10441 tggccatggt ccttgcggag aaccgaccgg ctgtgaaggc cgcgcagaac gagatctggc
    10501 agactttctt caggggccgc tacctgctcc tgcttatggg cctgttctcc atctacaccg
    10561 gcttcatcta caacgagtgc ttcagtcgcg ccaccagcat cttcccctcg ggctggagtg
    10621 tggccgccat ggccaaccag tctggctgga gtgatgcatt cctggcccag cacacgatgc
    10681 ttaccctgga tcccaacgtc accggtgtct tcctgggacc ctaccccttt ggcatcgatc
    10741 ctatttggag cctggctgcc aaccacttga gcttcctcaa ctccttcaag atgaagatgt
    10801 ccgtcatcct gggcgtcgtg cacatggcct ttggggtggt cctcggagtc ttcaaccacg
    10861 tgcactttgg ccagaggcac cggctgctgc tggagacgct gccggagctc accttcctgc
    10921 tgggactctt cggttacctc gtgttcctag tcatctacaa gtggctgtgt gtctgggctg
    10981 ccagggccgc ctcggccccc agcatcctca tccacttcat caacatgttc ctcttctccc
    11041 acagccccag caacaggctg ctctaccccc ggcaggaggt ggtccaggcc acgctggtgg
    11101 tcctggcctt ggccatggtg cccatcctgc tgcttggcac acccctgcac ctgctgcacc
    11161 gccaccgccg ccgcctgcgg aggaggcccg ctgaccgaca ggaggaaaac aaggccgggt
    11221 tgctggacct gcctgacgca tctgtgaatg gctggagctc cgatgaggaa aaggcagggg
    11281 gcctggatga tgaagaggag gccgagctcg tcccctccga ggtgctcatg caccaggcca
    11341 tccacaccat cgagttctgc ctgggctgcg tctccaacac cgcctcctac ctgcgcctgt
    11401 gggccctgag cctggcccac gcccagctgt ccgaggttct gtgggccatg gtgatgcgca
    11461 taggcctggg cctgggccgg gaggtgggcg tggcggctgt ggtgctggtc cccatctttg
    11521 ccgcctttgc cgtgatgacc gtggctatcc tgctggtgat ggagggactc tcagccttcc
    11581 tgcacgccct gcggctgcac tgggtggaat tccagaacaa gttctactca ggcacgggct
    11641 acaagctgag tcccttcacc ttcgctgcca cagatgacta g
//
