LOCUS       sequence-455042-        1173 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TIFA WT gene under control of a doxycycline-inducible promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Martin A, Caron S, Marcotte M, Bronnec P, Garneret E, Martel N, 
            Maalouf G, Seve P, Saadoun D, Jamilloux Y, Henry T
  TITLE     IFN-gamma licenses normal and pathogenic ALPK1/TIFA pathway in human
            monocytes.
  JOURNAL   iScience. 2024 Dec 10;28(1):111563. doi: 10.1016/j.isci.2024.111563.
            eCollection 2025 Jan 17.
  PUBMED    39868044
REFERENCE   2  (bases 1 to 1173)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2024-12-10"; volume: "28(1):111563. doi"; pages: " 
            10.1016/j.isci.2024.111563. eCollection 2025 Jan 17"
COMMENT     Sequence Label: pInducer20 TIFA WT
FEATURES             Location/Qualifiers
     source          1..1173
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             61..126
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(705..729)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             782..808
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 tttgtcaaaa gcaaggcttt aaggaaccaa ttcagtcgac tgggatccgg taccaccatg
       61 gactacaaag accatgacgg tgattataaa gatcatgaca tcgattacaa ggatgacgat
      121 gacaagcttg cggccgcgac ttccttcgag gacgccgata ctgaggaaac cgtcacctgc
      181 ctgcaaatga ccgtatatca cccaggtcaa ctccaatgcg gcatcttcca aagcatttcc
      241 ttcaatcgcg aaaagcttcc aagttctgag gtagtcaagt tcggtcgcaa ctctaatata
      301 tgccactaca ccttccaaga taagcaagtg tctcgggtac aattctccct tcaactcttc
      361 aagaagttta attctagtgt gctgagtttc gagattaaga acatgtcaaa gaaaactaac
      421 ttgattgtcg attctcggga attggggtat ttgaacaaga tggatctccc ttatcgctgt
      481 atggtgaggt ttggggaata ccaattcctt atggaaaagg aagacggaga atccctggag
      541 ttcttcgaaa cacagttcat cctgagtccc aggagcctgc tccaggagaa taattggccg
      601 ccgcatcgac caatccccga atacggaacc tactcactgt gttctagtca gtcttcttca
      661 cccaccgaga tggacgagaa cgaaagctag ctcgagatat ctagacccag ctttcttgta
      721 caaagtggtt gatatccagc acagtggcgg ccgctcgagt ctagagggcc cgcggttcga
      781 atacccatac gacgtcccag actacgctta gtaatgatta attaaactag aaattctacc
      841 gggtagggga ggcgcttttc ccaaggcagt ctggagtaac ccacccaaga tctggcctcc
      901 gcgccgggtt ttggcgcctc ccgcgggcgc ccccctcctc acggcgagcg ctgccacgtc
      961 agacgaaggg cgcagcgagc gtcctgatcc ttccgcccgg acgctcagga cagcggcccg
     1021 ctgctcataa gactcggcct tagaacccca gtatcagcag aaggacattt taggacggga
     1081 cttgggtgac tctagggcac tggttttctt tccagagagc ggaacaggcg aggaaaagta
     1141 gtcccttctc ggcgattctg cggagggatc tcc
//
