LOCUS       sequence-455043-        1164 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TIFA WT gene under control of a doxycycline-inducible promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Martin A, Caron S, Marcotte M, Bronnec P, Garneret E, Martel N, 
            Maalouf G, Seve P, Saadoun D, Jamilloux Y, Henry T
  TITLE     IFN-gamma licenses normal and pathogenic ALPK1/TIFA pathway in human
            monocytes.
  JOURNAL   iScience. 2024 Dec 10;28(1):111563. doi: 10.1016/j.isci.2024.111563.
            eCollection 2025 Jan 17.
  PUBMED    39868044
REFERENCE   2  (bases 1 to 1164)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2024-12-10"; volume: "28(1):111563. doi"; pages: " 
            10.1016/j.isci.2024.111563. eCollection 2025 Jan 17"
COMMENT     Sequence Label: pInducer20 TIFA WT
FEATURES             Location/Qualifiers
     source          1..1164
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(579..644)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(687..711)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(820..844)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(841..879)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    912..930
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    954..972
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    996..1014
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1038..1056
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1080..1098
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1122..1140
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 ggaataatct cgagctagct ttcgttctcg tccatctcgg tgggtgaaga agactgacta
       61 gaacacagtg agtaggttcc gtattcgggg attggtcgat gcggcggcca attattctcc
      121 tggagcaggc tcctgggact caggatgaac tgtgtttcga agaactccag ggattctccg
      181 tcttcctttt ccataaggaa ttggtattcc ccaaacctca ccatacagcg ataagggaga
      241 tccatcttgt tcaaataccc caattcccga gaatcgacaa tcaagttagt tttctttgac
      301 atgttcttaa tctcgaaact cagcacacta gaattaaact tcttgaagag ttgaagggag
      361 aattgtaccc gagacacttg cttatcttgg aaggtgtagt ggcatatatt agagttgcga
      421 ccgaacttga ctacctcaga acttggaagc ttttcgcgat tgaaggaaat gctttggaag
      481 atgccgcatt ggagttgacc tgggtgatat acggtcattt gcaggcaggt gacggtttcc
      541 tcagtatcgg cgtcctcgaa ggaagtcgcg gccgcaagct tgtcatcgtc atccttgtaa
      601 tcgatgtcat gatctttata atcaccgtca tggtctttgt agtccatggt ggtaccggat
      661 ccagtcgact gaattggttc ctttaaagcc tgcttttttg tacaaacttg ttgatatctg
      721 cagaattcca ccacactgga ctagttcggg gccgcggagg ctggatcggt cccggtgtct
      781 tctatggagg tcaaaacagc gtggatggcg tctccaggcg atctgacggt tcactaaacg
      841 agctctgctt atataggcct cccaccgtac acgcctacct cgacccgggt accgagctcg
      901 actttcactt ttctctatca ctgataggga gtggtaaact cgactttcac ttttctctat
      961 cactgatagg gagtggtaaa ctcgactttc acttttctct atcactgata gggagtggta
     1021 aactcgactt tcacttttct ctatcactga tagggagtgg taaactcgac tttcactttt
     1081 ctctatcact gatagggagt ggtaaactcg actttcactt ttctctatca ctgataggga
     1141 gtggtaaact cgactttcac tttt
//
