LOCUS       sequence-455247-        9523 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Empty backbone with araC-ParaBAD expression system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9523)
  AUTHORS   Intorcia V, Sava RL, Schroeder GP, Gebhardt MJ
  TITLE     A series of vectors for inducible gene expression in 
            multidrug-resistant Acinetobacter baumannii.
  JOURNAL   Appl Environ Microbiol. 2024 Sep 18;90(9):e0047424. doi: 
            10.1128/aem.00474-24. Epub 2024 Aug 20.
  PUBMED    39162403
REFERENCE   2  (bases 1 to 9523)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol."; date: "2024-09-18"; volume: "90(9):e0047424. 
            doi"; pages: " 10.1128/aem.00474-24. Epub 2024 Aug 20"
COMMENT     Sequence Label: pMApra-Para
FEATURES             Location/Qualifiers
     source          1..9523
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..840
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             827..1678
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     primer_bind     1946..1962
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     1947..1963
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(2055..2933)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     primer_bind     3167..3186
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     terminator      3377..3463
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3555..3582
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3601..3692
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4135..4237
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(4304..4323)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             4650..5450
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      5980..6374
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     oriT            6716..6803
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     CDS             8042..9013
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
ORIGIN
        1 atggctaccc ataagcctat caatattctg gaggcgttcg cagcagcgcc gccaccgctg
       61 gactacgttt tgcccaacat ggtggccggt acggtcgggg cgctggtgtc gcccggtggt
      121 gccggtaaat ccatgctggc cctgcaactg gccgcacaga ttgcaggcgg gccggatctg
      181 ctggaggtgg gcgaactgcc caccggcccg gtgatctacc tgcccgccga agacccgccc
      241 accgccattc atcaccgcct gcacgccctt ggggcgcacc tcagcgccga ggaacggcaa
      301 gccgtggctg acggcctgct gatccagccg ctgatcggca gcctgcccaa catcatggcc
      361 ccggagtggt tcgacggcct caagcgcgcc gccgagggcc gccgcctgat ggtgctggac
      421 acgctgcgcc ggttccacat cgaggaagaa aacgccagcg gccccatggc ccaggtcatc
      481 ggtcgcatgg aggccatcgc cgccgatacc gggtgctcta tcgtgttcct gcaccatgcc
      541 agcaagggcg cggccatgat gggcgcaggc gaccagcagc aggccagccg gggcagctcg
      601 gtactggtcg ataacatccg ctggcagtcc tacctgtcga gcatgaccag cgccgaggcc
      661 gaggaatggg gtgtggacga cgaccagcgc cggttcttcg tccgcttcgg tgtgagcaag
      721 gccaactatg gcgcaccgtt cgctgatcgg tggttcaggc ggcatgacgg cggggtgctc
      781 aagcccgccg tgctggagag gcagcgcaag agcaaggggg tgccccgtgg tgaagcctaa
      841 gaacaagcac agcctcagcc acgtccggca cgacccggcg cactgtctgg cccccggcct
      901 gttccgtgcc ctcaagcggg gcgagcgcaa gcgcagcaag ctggacgtga cgtatgacta
      961 cggcgacggc aagcggatcg agttcagcgg cccggagccg ctgggcgctg atgatctgcg
     1021 catcctgcaa gggctggtgg ccatggctgg gcctaatggc ctagtgcttg gcccggaacc
     1081 caagaccgaa ggcggacggc agctccggct gttcctggaa cccaagtggg aggccgtcac
     1141 cgctgatgcc atggtggtca aaggtagcta tcgggcgctg gcaaaggaaa tcggggcaga
     1201 ggtcgatagt ggtggggcgc tcaagcacat acaggactgc atcgagcgcc tttggaaggt
     1261 atccatcatc gcccagaatg gccgcaagcg gcaggggttt cggctgctgt cggagtacgc
     1321 cagcgacgag gcggacgggc gcctgtacgt ggccctgaac cccttgatcg cgcaggccgt
     1381 catgggtggc ggccagcatg tgcgcatcag catggacgag gtgcgggcgc tggacagcga
     1441 aaccgcccgc ctgctgcacc agcggctgtg tggctggatc gaccccggca aaaccggcaa
     1501 ggcttccata gataccttgt gcggctatgt ctggccgtca gaggccagtg gttcgaccat
     1561 gcgcaagcgc cgccagcggg tgcgcgaggc gttgccggag ctggtcgcgc tgggctggac
     1621 ggtaaccgag ttcgcggcgg gcaagtacga catcacccgg cccaaggcgg caggctgacc
     1681 ccccccactc tattgtaaac aagacatttt tatcttttat attcaatggc ttattttcct
     1741 gctaattggt aataccatga aaaataccat gctcagaaaa ggcttaacaa tattttgaaa
     1801 aattgcctac tgagcgctgc cgcacagctc cataggccgc tttcctggct ttgcttccag
     1861 atgtatgctc ttctgctccc gaacgccagc aagacgtagc ccagcgcgtc ggccagcttg
     1921 caattcgcgc taacttacat taatctcgag gtcgacggta tcgatgcata atgtgcctgt
     1981 caaatggacg aagcagggat tctgcaaacc ctatgctact ccgtcaagcc gtcaattgtc
     2041 tgattcgtta ccaattatga caacttgacg gctacatcat tcactttttc ttcacaaccg
     2101 gcacggaact cgctcgggct ggccccggtg cattttttaa atacccgcga gaaatagagt
     2161 tgatcgtcaa aaccaacatt gcgaccgacg gtggcgatag gcatccgggt ggtgctcaaa
     2221 agcagcttcg cctggctgat acgttggtcc tcgcgccagc ttaagacgct aatccctaac
     2281 tgctggcgga aaagatgtga cagacgcgac ggcgacaagc aaacatgctg tgcgacgctg
     2341 gcgatatcaa aattgctgtc tgccaggtga tcgctgatgt actgacaagc ctcgcgtacc
     2401 cgattatcca tcggtggatg gagcgactcg ttaatcgctt ccatgcgccg cagtaacaat
     2461 tgctcaagca gatttatcgc cagcagctcc gaatagcgcc cttccccttg cccggcgtta
     2521 atgatttgcc caaacaggtc gctgaaatgc ggctggtgcg cttcatccgg gcgaaagaac
     2581 cccgtattgg caaatattga cggccagtta agccattcat gccagtaggc gcgcggacga
     2641 aagtaaaccc actggtgata ccattcgcga gcctccggat gacgaccgta gtgatgaatc
     2701 tctcctggcg ggaacagcaa aatatcaccc ggtcggcaaa caaattctcg tccctgattt
     2761 ttcaccaccc cctgaccgcg aatggtgaga ttgagaatat aacctttcat tcccagcggt
     2821 cggtcgataa aaaaatcgag ataaccgttg gcctcaatcg gcgttaaacc cgccaccaga
     2881 tgggcattaa acgagtatcc cggcagcagg ggatcatttt gcgcttcagc catacttttc
     2941 atactcccgc cattcagaga agaaaccaat tgtccatatt gcatcagaca ttgccgtcac
     3001 tgcgtctttt actggctctt ctcgctaacc aaaccggtaa ccccgcttat taaaagcatt
     3061 ctgtaacaaa gcgggaccaa agccatgaca aaaacgcgta acaaaagtgt ctataatcac
     3121 ggcagaaaag tccacattga ttatttgcac ggcgtcacac tttgctatgc catagcattt
     3181 ttatccataa gattagcgga tcctacctga cgctttttat cgcaactctc tactgtttct
     3241 agagacgtcg aattcctgca gaagcttact agtggctgaa tcccgaaaca gttcgcaggt
     3301 aatagttaga gcctgcataa cggtttcggg attttttata ggtctcccca tgcgagagta
     3361 gggaactgcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
     3421 tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccgg gagcggattt
     3481 gaacgttgcg aagcaacggc ccggagggtg gcgggcagga cgcccgccat aaactgccag
     3541 gcatcaaatt aagcagaagg ccatcctgac ggatggcctt tttgcgtttc tacaaactct
     3601 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     3661 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     3721 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     3781 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     3841 acagcggtaa gatccttgag agttttcgcc ccgaagaacg tgatgtccgg cggtgctttt
     3901 gccgttacgc accaccccgt cagtagctga acaggaggga cagctgatag aaacagaagc
     3961 cactggcctc aaaaacacca tcatacacta aatcagtaag ttggcagcat cacccgacgc
     4021 actttgcgcc gaataaatac ctgtgacgga agatcacttc gcagaataaa taaatcctgg
     4081 tgtccctgtt gataccggga agccctgggc caacttttgg cgaaaatgag acgttgatcg
     4141 gcacgtaaga ggttccaact ttcaccataa tgaaataaga tcactaccgg gcgtattttt
     4201 tgagttatcg agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata
     4261 taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt
     4321 tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt tatcaaggcc
     4381 cgatccttgg agcccttgcc ctcccgcacg atgatcgtgc cgtgatcgaa atccagatcc
     4441 ttgacccgca gttgcaaacc ctcactgatc cggctcacgg taactgatgc cgtatttgca
     4501 gtaccagcgt acggcccaca gaatgatgtc acgctgaaaa tgccggcctt tgaatgggtt
     4561 catgtgcagc tccatcagca aaaggggatg ataagtttat caccacctac tatttgcaac
     4621 agtgccgttg atcgtgctat gatcgactga tgtcatcagc ggtggagtgc aatgtcgtgc
     4681 aatacgaatg gcgaaaagcc gagctcatcg gtcagcttct caaccttggg gttacccccg
     4741 gcggtgtgct gctggtccac agctccttcc gtagcgtccg gcccctcgaa gatgggccac
     4801 ttggactgat cgaggccctg cgtgctgcgc tgggtccggg agggacgctc gtcatgccct
     4861 cgtggtcagg tctggacgac gagccgttcg atcctgccac gtcgcccgtt acaccggacc
     4921 ttggagttgt ctctgacaca ttctggcgcc tgccaaatgt aaagcgcagc gcccatccat
     4981 ttgcctttgc ggcagcgggg ccacaggcag agcagatcat ctctgatcca ttgcccctgc
     5041 cacctcactc gcctgcaagc ccggtcgccc gtgtccatga actcgatggg caggtacttc
     5101 tcctcggcgt gggacacgat gccaacacga cgctgcatct tgccgagttg atggcaaagg
     5161 ttccctatgg ggtgccgaga cactgcacca ttcttcagga tggcaagttg gtacgcgtcg
     5221 attatctcga gaatgaccac tgctgtgagc gctttgcctt ggcggacagg tggctcaagg
     5281 agaagagcct tcagaaggaa ggtccagtcg gtcatgcctt tgctcggttg atccgctccc
     5341 gcgacattgt ggcgacagcc ctgggtcaac tgggccgaga tccgttgatc ttcctgcatc
     5401 cgccagaggc gggatgcgaa gaatgcgatg ccgctcgcca gtcgattggc tgagctcatg
     5461 aaaacgtggc caatatggac aacttcttcg cccccgtttt caccatgggc aaatattata
     5521 cgcaaggcga caaggtgctg atgccgctgg cgattcaggt tcatcatgcc gtttgtgatg
     5581 gcttccatgt cggcagaatg cttaatgaat tacaacagta ctgcgatgag tggcagggcg
     5641 gggcgtaatt tttttaaggc agttattgtc ttcaaattcc cgttgcacat agcccggcaa
     5701 ttcctttccc tgctctgcca taagcgcagc gaatgccggg taatactcgt caacgatctg
     5761 atagagaagg gtttgctcgg gtcggtggct ctggtaacga ccagtatccc gatcccggct
     5821 ggccgtcctg gccgccacat gaggcatgtt ccgcgtcctt gcaatactgt gtttacatac
     5881 agtctatcgc ttagcggaaa gttcttttac cctcagccga aatgcctgcc gttgctagac
     5941 attgccagcc agtgcccgtc actcccgtac taactgtcac gaacccctgc aataactgtc
     6001 acgcccccct gcaataactg tcacgaaccc ctgcaataac tgtcacgccc ccaaacctgc
     6061 aaacccagca ggggcggggg ctggcggggt gttggaaaaa tccatccatg attatctaag
     6121 aataatccac taggcgcggt tatcagcgcc cttgtggggc gctgctgccc ttgcccaata
     6181 tgcccggcca gaggccggat agctggtcta ttcgctgcgc taggctacac accgccccac
     6241 cgctgcgcgg cagggggaaa ggcgggcaaa gcccgctaaa ccccacacca aaccccgcag
     6301 aaatacgctg gagcgctttt agccgcttta gcggcctttc cccctacccg aagggtgggg
     6361 ccgcgtgtgc agccccgcag ggcctgtctc ggtcgatcat tcagcccggc tcatccttct
     6421 ggcgtggcgg cagaccgaac aaggcgcggt cgtggtcgcg ttcaaggtac gcatccattg
     6481 ccgccatgag ccgatcctcc ggccactcgc tgctgttcac cttggccaaa atcatggccc
     6541 ccaccagcac cttgcgcctt gtttcgttct tgcgctcttg ctgctgttcc cttgcccgca
     6601 cccgctgaat ttcggcattg attcgcgctc gttgttcttc gagcttggcc agccgatccg
     6661 ccgccttgtt gctcccctta accatcttga caccccattg ttaatgtgct gtctcgtagg
     6721 ctatcatgga ggcacagcgg cggcaatccc gaccctactt tgtaggggag ggcgcactta
     6781 ccggtttctc ttcgagaaac tggcctaacg gccacccttc gggcggtgcg ctctccgagg
     6841 gccattgcat ggagccgaaa agcaaaagca acagcgaggc agcatggcga tttatcacct
     6901 tacggcgaaa accggcagca ggtcgggcgg ccaatcggcc agggccaagg ccgactacat
     6961 ccagcgcgaa ggcaagtatg cccgcgacat ggatgaagtc ttgcacgccg aatccgggca
     7021 catgccggag ttcgtcgagc ggcccgccga ctactgggat gctgccgacc tgtatgaacg
     7081 cgccaatggg cggctgttca aggaggtcga atttgccctg ccggtcgagc tgaccctcga
     7141 ccagcagaag gcgctggcgt ccgagttcgc ccagcacctg accggtgccg agcgcctgcc
     7201 gtatacgctg gccatccatg ccggtggcgg cgagaacccg cactgccacc tgatgatctc
     7261 cgagcggatc aatgacggca tcgagcggcc cgccgctcag tggttcaagc ggtacaacgg
     7321 caagaccccg gagaagggcg gggcacagaa gaccgaagcg ctcaagccca aggcatggct
     7381 tgagcagacc cgcgaggcat gggccgacca tgccaaccgg gcattagagc gggctggcca
     7441 cgacgcccgc attgaccaca gaacacttga ggcgcagggc atcgagcgcc tgcccggtgt
     7501 tcacctgggg ccgaacgtgg tggagatgga aggccggggc atccgcaccg accgggcaga
     7561 cgtggccctg aacatcgaca ccgccaacgc ccagatcatc gacttacagg aataccggga
     7621 ggcaatagac catgaacgca atcgacagag tgaagaaatc cagaggcatc aacgagttag
     7681 cggagcagat cgaaccgctg gcccagagca tggcgacact ggccgacgaa gcccggcagg
     7741 tcatgagcca gacccagcag gccagcgagg cgcaggcggc ggagtggctg aaagcccagc
     7801 gccagacagg ggcggcatgg gtggagctgg ccaaagagtt gcgggaggta gccgccgagg
     7861 tgagcagcgc cgcgcagagc gcccggagcg cgtcgcgggg gtggcactgg aagctatggc
     7921 taaccgtgat gctggcttcc atgatgccta cggtggtgct gctgatcgca tcgttgctct
     7981 tgctcgacct gacgccactg acaaccgagg acggctcgat ctggctgcgc ttggtggccc
     8041 gatgaagaac gacaggactt tgcaggccat aggccgacag ctcaaggcca tgggctgtga
     8101 gcgcttcgat atcggcgtca gggacgccac caccggccag atgatgaacc gggaatggtc
     8161 agccgccgaa gtgctccaga acacgccatg gctcaagcgg atgaatgccc agggcaatga
     8221 cgtgtatatc aggcccgccg agcaggagcg gcatggtctg gtgctggtgg acgacctcag
     8281 cgagtttgac ctggatgaca tgaaagccga gggccgggag cctgccctgg tagtggaaac
     8341 cagcccgaag aactatcagg catgggtcaa ggtggccgac gccgcaggcg gtgaacttcg
     8401 ggggcagatt gcccggacgc tggccagcga gtacgacgcc gacccggcca gcgccgacag
     8461 ccgccactat ggccgcttgg cgggcttcac caaccgcaag gacaagcaca ccacccgcgc
     8521 cggttatcag ccgtgggtgc tgctgcgtga atccaagggc aagaccgcca ccgctggccc
     8581 ggcgctggtg cagcaggctg gccagcagat cgagcaggcc cagcggcagc aggagaaggc
     8641 ccgcaggctg gccagcctcg aactgcccga gcggcagctt agccgccacc ggcgcacggc
     8701 gctggacgag taccgcagcg agatggccgg gctggtcaag cgcttcggtg atgacctcag
     8761 caagtgcgac tttatcgccg cgcagaagct ggccagccgg ggccgcagtg ccgaggaaat
     8821 cggcaaggcc atggccgagg ccagcccagc gctggcagag cgcaagcccg gccacgaagc
     8881 ggattacatc gagcgcaccg tcagcaaggt catgggtctg cccagcgtcc agcttgcgcg
     8941 ggccgagctg gcacgggcac cggcaccccg ccagcgaggc atggacaggg gcgggccaga
     9001 tttcagcatg tagtgcttgc gttggtactc acgcctgtta tactatgagt actcacgcac
     9061 agaagggggt tttatggaat acgaaaaaag cgcttcaggg tcggtctacc tgatcaaaag
     9121 tgacaagggc tattggttgc ccggtggctt tggttatacg tcaaacaagg ccgaggctgg
     9181 ccgcttttca gtcgctgata tggccagcct taaccttgac ggctgcacct tgtccttgtt
     9241 ccgcgaagac aagcctttcg gccccggcaa gtttctcggt gactgatatg aaagaccaaa
     9301 aggacaagca gaccggcgac ctgctggcca gccctgacgc tgtacgccaa gcgcgatatg
     9361 ccgagcgcat gaaggccaaa gggatgcgtc agcgcaagtt ctggctgacc gacgacgaat
     9421 acgaggcgct gcgcgagtgc ctggaagaac tcagagcggc gcagggcggg ggtagtgacc
     9481 ccgccagcgc ctaaccacca actgcctgca aaggaggcaa tca
//
