LOCUS       sequence-455259-        9734 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CasRx protein for RNA targeting in piggyBac backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9734)
  AUTHORS   Wei J, Lotfy P, Faizi K, Baungaard S, Gibson E, Wang E, Slabodkin H,
            Kinnaman E, Chandrasekaran S, Kitano H, Durrant MG, Duffy CV, Pawluk
            A, Hsu PD, Konermann S
  TITLE     Deep learning and CRISPR-Cas13d ortholog discovery for optimized RNA
            targeting.
  JOURNAL   Cell Syst. 2023 Dec 20;14(12):1087-1102.e13. doi: 
            10.1016/j.cels.2023.11.006. Epub 2023 Dec 12.
  PUBMED    38091991
REFERENCE   2  (bases 1 to 9734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2023.11.006"; journalName: "Cell Syst"; date: 
            "2023-12-20- 20"; volume: "14"; issue: "12"; pages: "1087-1102.e13"
COMMENT     Sequence Label: PB_EF1a-CasRx-msfGFP-2A-Blast
FEATURES             Location/Qualifiers
     source          1..9734
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     repeat_region   1052..1086
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        1411..2589
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1642..2580
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2537..2557
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             2608..2628
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2638..5535
                     /codon_start=1
                     /product="RNA-targeting Type VI CRISPR protein from 
                     Ruminococcus flavefaciens strain XPD3002 (Konermann et al.,
                     2018)"
                     /label=RfxCas13d
                     /translation="IEKKKSFAKGMGVKSTLVSGSKVYMTTFAEGSDARLEKIVEGDSI
                     RSVNEGEAFSAEMADKNAGYKIGNAKFSHPKGYAVVANNPLYTGPVQQDMLGLKETLEK
                     RYFGESADGNDNICIQVIHNILDIEKILAEYITNAAYAVNNISGLDKDIIGFGKFSTVY
                     TYDEFKDPEHHRAAFNNNDKLINAIKAQYDEFDNFLDNPRLGYFGQAFFSKEGRNYIIN
                     YGNECYDILALLSGLRHWVVHNNEEESRISRTWLYNLDKNLDNEYISTLNYLYDRITNE
                     LTNSFSKNSAANVNYIAETLGINPAEFAEQYFRFSIMKEQKNLGFNITKLREVMLDRKD
                     MSEIRKNHKVFDSIRTKVYTMMDFVIYRYYIEEDAKVAAANKSLPDNEKSLSEKDIFVI
                     NLRGSFNDDQKDALYYDEANRIWRKLENIMHNIKEFRGNKTREYKKKDAPRLPRILPAG
                     RDVSAFSKLMYALTMFLDGKEINDLLTTLINKFDNIQSFLKVMPLIGVNAKFVEEYAFF
                     KDSAKIADELRLIKSFARMGEPIADARRAMYIDAIRILGTNLSYDELKALADTFSLDEN
                     GNKLKKGKHGMRNFIINNVISNKRFHYLIRYGDPAHLHEIAKNEAVVKFVLGRIADIQK
                     KQGQNGKNQIDRYYETCIGKDKGKSVSEKVDALTKIITGMNYDQFDKKRSVIEDTGREN
                     AEREKFKKIISLYLTVIYHILKNIVNINARYVIGFHCVERDAQLYKEKGYDINLKKLEE
                     KGFSSVTKLCAGIDETAPDKRKDVEKEMAERAKESIDSLESANPKLYANYIKYSDEKKA
                     EEFTRQINREKAKTALNAYLRNTKWNVIIREDLLRIDNKTCTLFRNKAVHLEVARYVHA
                     YINDIAEVNSYFQLYHYIMQRIIMNERYEKSSGKVSEYFDAVNDEKKYNDRLLKLLCVP
                     FGYCIPRFKNLSIEALFDRNEAAKFDKEKKKVSGNS"
     CDS             6310..6330
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6340..6366
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6376..6432
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6433..6831
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    6847..7435
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6900..6920)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7318..7329)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7605..7624)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   7806..7868
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7934..7953)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7935..7953)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7960..7977)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7960..7976)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7969..7991)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8118..8573
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8205..8224)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8415..8436
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8599..8703
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8704..8772,8773..9564)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8922..8941)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctcg aaattaaccc
     1021 tcactaaagg gaacaaaagc tgggtactta accctagaaa gatagtctgc gtaaaattga
     1081 cgcatgcatt cttgaaatat tgctctctct ttctaaatag cgcgaatccg tcgctgtgca
     1141 tttaggacat ctcagtcgcc gcttggagct cccgtgaggc gtgcttgtca atgcggtaag
     1201 tgtcactgat tttgaactat aacgaccgcg tgagtcaaaa tgacgcatga ttatctttta
     1261 cgtgactttt aagatttaac tcatacgata attatattgt tatttcatgt tctacttacg
     1321 tgataactta ttatatatat attttcttgt tatagatatc ggaatctttg cagctaatgg
     1381 accttctagg tcttgaaagg agtgggaatt ggctccggtg cccgtcagtg ggcagagcgc
     1441 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac cggtgcctag
     1501 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc
     1561 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac
     1621 gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt
     1681 acgggttatg gcccttgcgt gccttgaatt acttccacct ggctgcagta cgtgattctt
     1741 gatcccgagc ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc
     1801 ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg cgtgcgaatc
     1861 tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt ctctagccat ttaaaatttt
     1921 tgatgacctg ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc gggccaagat
     1981 ctgcacactg gtatttcggt ttttggggcc gcgggcggcg acggggcccg tgcgtcccag
     2041 cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg acgggggtag
     2101 tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc
     2161 tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag cggaaagatg gccgcttccc
     2221 ggccctgctg cagggagctc aaaatggagg acgcggcgct cgggagagcg ggcgggtgag
     2281 tcacccacac aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg tgactccacg
     2341 gagtaccggg cgccgtccag gcacctcgat tagttctcga gcttttggag tacgtcgtct
     2401 ttaggttggg gggaggggtt ttatgcgatg gagtttcccc acactgagtg ggtggagact
     2461 gaagttaggc cagcttggca cttgatgtaa ttctccttgg aatttgccct ttttgagttt
     2521 ggatcttggt tcattctcaa gcctcagaca gtggttcaaa gtttttttct tccatttcag
     2581 gtgtcgtgac gtacggccac catgagcccc aagaagaaga gaaaggtgga ggccagcatc
     2641 gagaagaaga agagcttcgc caagggcatg ggcgtgaaga gcaccctggt gtccggctct
     2701 aaggtgtaca tgaccacatt tgccgagggc tccgacgcac ggctggagaa gatcgtggag
     2761 ggcgatagca tcagatccgt gaacgaggga gaggccttca gcgccgagat ggccgacaag
     2821 aatgccggct ataagatcgg caacgccaag ttttcccacc caaagggcta tgccgtggtg
     2881 gccaacaatc cactgtacac cggaccagtg cagcaggaca tgctgggcct gaaggagaca
     2941 ctggagaaga gatacttcgg cgagagcgcc gacggcaatg ataacatctg catccaggtc
     3001 atccacaata tcctggacat cgagaagatc ctggccgagt atatcaccaa cgccgcctac
     3061 gccgtgaaca atatctccgg cctggacaag gacatcatcg gcttcggcaa gttttctacc
     3121 gtgtacacct acgacgagtt caaggacccc gagcaccaca gggccgcctt taacaataac
     3181 gacaagctga tcaatgccat caaggcccag tacgacgagt tcgataattt tctggataac
     3241 ccccggctgg gctattttgg ccaggccttc ttttctaagg agggcagaaa ctatatcatc
     3301 aattacggca acgagtgtta cgacatcctg gccctgctga gcggcctgag gcactgggtg
     3361 gtgcacaata acgaggagga gtctaggatc agccgcacct ggctgtataa tctggacaag
     3421 aatctggata acgagtacat ctccacactg aactacctgt atgatcggat caccaatgag
     3481 ctgacaaaca gcttctccaa gaacagcgcc gccaatgtga actacatcgc cgagacactg
     3541 ggcatcaacc ccgccgagtt cgccgagcag tacttccggt tcagcatcat gaaggagcag
     3601 aagaatctgg gctttaacat cacaaagctg cgggaagtga tgctggacag aaaggatatg
     3661 tccgagatcc ggaagaacca caaggtgttc gattctatca gaaccaaggt gtatacaatg
     3721 atggactttg tgatctaccg gtactatatc gaggaggatg ccaaggtggc tgccgccaat
     3781 aagagcctgc ccgacaacga gaagtctctg agcgagaagg acatcttcgt gatcaatctg
     3841 agaggctcct ttaacgacga tcagaaggac gccctgtact atgatgaggc caatcggatc
     3901 tggagaaagc tggagaatat catgcacaac atcaaggagt tcaggggcaa caagacccgc
     3961 gagtacaaga agaaggacgc accaaggctg cctcgcatcc tgcctgcagg aagggacgtg
     4021 agcgccttca gcaagctgat gtatgccctg acaatgtttc tggacggcaa ggagatcaat
     4081 gatctgctga ccacactgat caataagttc gacaacatcc agtcctttct gaaagtgatg
     4141 cctctgatcg gcgtgaacgc caagttcgtg gaggagtacg ccttctttaa ggacagcgcc
     4201 aagatcgccg atgagctgag gctgatcaag tcctttgcca ggatgggaga gccaatcgca
     4261 gacgcaagga gagccatgta catcgatgcc atcagaatcc tgggcaccaa tctgtcttat
     4321 gatgagctga aggccctggc cgacaccttc agcctggacg agaatggcaa caagctgaag
     4381 aagggcaagc acggcatgcg caatttcatc atcaataacg tgatcagcaa caagaggttt
     4441 cactatctga tcagatacgg cgaccctgca cacctgcacg agatcgccaa gaacgaggcc
     4501 gtggtgaagt tcgtgctggg caggatcgcc gacatccaga agaagcaggg ccagaatggc
     4561 aagaaccaga tcgaccgcta ctatgagaca tgcatcggca aggataaggg caagtccgtg
     4621 tctgagaagg tggacgccct gaccaagatc atcacaggca tgaattatga ccagttcgat
     4681 aagaagcgga gcgtgatcga ggacaccgga agggagaacg cagagagaga gaagtttaag
     4741 aagatcatca gcctgtatct gacagtgatc taccacatcc tgaagaatat cgtgaatatc
     4801 aacgccagat acgtgatcgg cttccactgc gtggagcgcg atgcccagct gtataaggag
     4861 aagggctacg acatcaacct gaagaagctg gaggagaagg gctttagctc cgtgaccaag
     4921 ctgtgcgcag gaatcgacga gacagcaccc gacaagcgga aggatgtgga gaaggagatg
     4981 gccgagagag ccaaggagag catcgactcc ctggagtctg ccaatcctaa gctgtacgcc
     5041 aactatatca agtactccga tgagaagaag gccgaggagt tcacccggca gatcaataga
     5101 gagaaggcca agaccgccct gaacgcctac ctgcggaata caaagtggaa cgtgatcatc
     5161 agggaggacc tgctgcgcat cgataataag acctgtacac tgttcaggaa caaggccgtg
     5221 cacctggagg tggcccgcta tgtgcacgcc tacatcaatg acatcgccga ggtgaactcc
     5281 tactttcagc tgtaccacta tatcatgcag cggatcatca tgaacgagag atatgagaag
     5341 tctagcggca aggtgtctga gtacttcgac gccgtgaatg atgagaagaa gtataacgat
     5401 agactgctga agctgctgtg cgtgcctttc ggctactgta tcccaaggtt taagaatctg
     5461 agcatcgagg ccctgttcga caggaacgag gcagccaagt ttgataagga gaagaagaag
     5521 gtgagcggca actcctctag aggaggaggt ggaagcggag gaggaggaag cggaggagga
     5581 ggtagcgtga gtaaaggtga agaactcttc actggagtag tgcccattct ggtagagctt
     5641 gatggagatg taaatggaca taaattctcc gtcaggggcg aaggcgaagg ggacgccacg
     5701 aatggtaagc tgactctgaa attcatctgt acgacgggca aactgcccgt cccatggcct
     5761 acactcgtaa cgaccctcac ctacggcgtg caatgctttt ctcgatatcc cgaccacatg
     5821 aaacagcatg actttttcaa gtctgcaatg cctgaaggtt atgttcaaga aaggaccatc
     5881 agctttaagg atgatggtac atataaaacc cgagccgagg ttaaatttga aggggacact
     5941 ctggttaatc gaattgaact gaaaggtatt gattttaagg aggacggtaa catactgggg
     6001 cacaagttgg agtacaactt taacagccat aatgtgtata ttaccgctga taagcagaaa
     6061 aatgggataa aggccaactt taagatccga cataatgtcg aagatggtag tgttcaactg
     6121 gctgatcatt accaacaaaa tacgcccatc ggagatggac ctgtactctt gcctgacaat
     6181 cattatctct ccacgcaatc aaagctttcc aaggacccaa acgaaaagag agatcacatg
     6241 gtccttctgg aatttgtgac tgccgcaggc atcactctcg gtatggatga gctgtacaag
     6301 ggatccggac ctaagaaaaa gaggaaggtg gcggccgctt acccatacga tgttccagat
     6361 tacgctggat ccggcgcaac aaacttctct ctgctgaaac aagccggaga tgtcgaagag
     6421 aatcctggac cgatggccaa gcctttgtct caagaagaat ccaccctcat tgaaagagca
     6481 acggctacaa tcaacagcat ccccatctct gaagactaca gcgtcgccag cgcagctctc
     6541 tctagcgacg gccgcatctt cactggtgtc aatgtatatc attttactgg gggaccttgt
     6601 gcagaactcg tggtgctggg cactgctgct gctgcggcag ctggcaacct gacttgtatc
     6661 gtcgcgatcg gaaatgagaa caggggcatc ttgagcccct gcggacggtg ccgacaggtg
     6721 cttctcgatc tgcatcctgg gatcaaagcc atagtgaagg acagtgatgg acagccgacg
     6781 gcagttggga ttcgtgaatt gctgccctct ggttatgtgt gggagggcta aacgcgttaa
     6841 gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     6901 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     6961 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     7021 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     7081 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     7141 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     7201 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     7261 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     7321 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     7381 cgtcttcgcc ttcgccctca gacgagtcgg atttcccttt gggccgcctc cccgcgtcga
     7441 ctttaagacc aatgacttac aaggcataat cagccatacc acatttgtag aggttttact
     7501 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
     7561 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     7621 tttcacaaat gctagctttt gttactttat agaagaaatt ttgagttttt gttttttttt
     7681 aataaataaa taaacataaa taaattgttt gttgaattta ttattagtat gtaagtgtaa
     7741 atataataaa acttaatatc tattcaaatt aataaataaa cctcgatata cagaccgata
     7801 aaacacatgc gtcaatttta cgcatgatta tctttaacgt acgtcacaat atgattatct
     7861 ttctagggtt aaataatagt ttctaatttt tttattattc agcctgctgt cgtgaatacc
     7921 gagctccaat tcgccctata gtgagtcgta ttacaattca ctggccgtcg ttttacaacg
     7981 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     8041 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     8101 cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     8161 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     8221 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     8281 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     8341 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     8401 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     8461 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     8521 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc
     8581 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     8641 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     8701 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     8761 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     8821 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     8881 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     8941 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     9001 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     9061 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     9121 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     9181 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     9241 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     9301 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     9361 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     9421 tcccgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     9481 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     9541 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     9601 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     9661 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     9721 atcaaaggat cttc
//
