LOCUS       sequence-455284-       10462 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pORTMAGE-Ec1 + supplement E. coli SSB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10462)
  AUTHORS   Fishman CB, Crawford KD, Bhattarai-Kline S, Poola D, Zhang K, 
            Gonzalez-Delgado A, Rojas-Montero M, Shipman SL
  TITLE     Continuous multiplexed phage genome editing using recombitrons.
  JOURNAL   Nat Biotechnol. 2024 Sep 5. doi: 10.1038/s41587-024-02370-5.
  PUBMED    39237706
REFERENCE   2  (bases 1 to 10462)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2024 Sep 5. doi: 10.1038/s41587-024-02370-5."
COMMENT     Sequence Label: pCF.111
FEATURES             Location/Qualifiers
     source          1..10462
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             1890..1901
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      2495..2589
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(2710..2729)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2721..3536
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     oriT            3703..3811
                     /note="incP origin of transfer"
     CDS             complement(3822..4673)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(4660..5499)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(6010..6981)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     rep_origin      7088..7482
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     terminator      complement(7520..7606)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(7631..8596)
                     /codon_start=1
                     /gene="xylS"
                     /product="XylS regulator encoded by the Pseudomonas putida 
                     TOL plasmid pWWO"
                     /label=XylS
                     /note="stimulates transcription from the Pm promoter in the
                     presence of aromatic compounds such as m-toluate or 
                     benzoate"
                     /translation="MDFCLLNEKSQIFVHAEPYAVSDYVNQYVGTHSIRLPKGGRPAGR
                     LHHRIFGCLDLCRISYGGSVRVISPGLETCYHLQIILKGHCLWRGHGQEHYFAPGELLL
                     LNPDDQADLTYSEDCEKFIVKLPSVVLDRACSDNNWHKPREGIRFAARHNLQQLDGFIN
                     LLGLVCDEAEHTKSMPRVQEHYAGIIASKLLEMLGSNVSREIFSKGNPSFERVVQFIEE
                     NLKRNISLERLAELAMMSPRSLYNLFEKHAGTTPKNYIRNRKLESIRACLNDPSANVRS
                     ITEIALDYGFLHLGRFAENYRSAFGELPSDTLRQCKKEVA"
     promoter        9496..9576
                     /label=Pm promoter
                     /note="The bacterial Pm promoter is activated by XylS in 
                     the presence of benzoate or m-toluate (Marques et al., 
                     1999)."
ORIGIN
        1 gatccaggag aatacacatg ccaattcagg tcttaccgcc acaactggcg aaccagattg
       61 ccgcaggtga ggtggtcgag cgacctgcgt cggtagtcaa agaactagtg aaaaacagcc
      121 tcgatgcagg tgcgacgcgt atcgatattg atatcgaacg cggtggggcg aaacttatcc
      181 gcattcgtga taacggctgc ggtatcaaaa aagatgagct ggcgctggcg ctggctcgtc
      241 atgccaccag taaaatcgcc tctctggacg atctcgaagc cattatcagc ctgggctttc
      301 gcggtgaggc gctggcgagt atcagttcgg tttcccgcct gacgctcact tcacgcaccg
      361 cagaacagca ggaagcctgg caggcctatg ccgaagggcg cgatatgaac gtgacggtaa
      421 aaccggcggc gcatcctgtg gggacgacgc tggaggtgct ggatctgttc tacaacaccc
      481 cggcgcggcg caaattcctg cgcaccgaga aaaccgaatt taaccacatt gatgagatca
      541 tccgccgcat tgcgctggcg cgtttcgacg tcacgatcaa cctgtcgcat aacggtaaaa
      601 ttgtgcgtca gtaccgcgca gtgccggaag gcgggcaaaa agaacggcgc ttaggcgcga
      661 tttgcggcac cgcttttctt gaacaagcgc tggcgattga atggcaacac ggcgatctca
      721 cgctacgcgg ctgggtggcc gatccaaatc acaccacgcc cgcactggca gaaattcagt
      781 attgctacgt gaacggtcgc atgatgcgcg atcgcctgat caatcacgcg atccgccagg
      841 cctgcgaaga caaactgggg gccgatcagc aaccggcatt tgtgttgtat ctggagatcg
      901 acccacatca ggtggacgtc aacgtgcacc ccgccaaaca cgaagtgcgt ttccatcagt
      961 cgcgtctggt gcatgatttt atctatcagg gcgtgctgag cgtgctacaa cagcaactgg
     1021 aaacgccgct accgctggac gatgaacccc aacctgcacc gcgttccatt ccggaaaacc
     1081 gcgtggcggc ggggcgcaat cactttgcag aaccggcagc tcgtgagccg gtagctccgc
     1141 gctacactcc tgcgccagca tcaggcagtc gtccggctgc cccctggccg aatgcgcagc
     1201 caggctacca gaaacagcaa ggtgaagtgt atcgccagct tttgcaaacg cccgcgccga
     1261 tgcaaaaatt aaaagcgccg gaaccgcagg aacctgcact tgcggcgaac agtcagagtt
     1321 ttggtcgggt actgactatc gtccattccg actgtgcgtt gctggagcgc gacggcaaca
     1381 tttcactttt atccttgcca gtggcagaac gttggctgcg tcaggcacaa ttgacgccgg
     1441 gtgaagcgcc cgtttgcgcc cagccgctgc tgattccgtt gcggctaaaa gtttctgccg
     1501 aagaaaaatc ggcattagaa aaagcgcagt ctgccctggc ggaattgggt attgatttcc
     1561 agtcagatgc acagcatgtg accatcaggg cagtgccttt acccttacgc caacaaaatt
     1621 tacaaatctt gattcctgaa ctgataggct acctggcgaa gcagtccgta ttcgaacctg
     1681 gcaatattgc gcagtggatt gcacgaaatc tgatgagcga acatgcgcag tggtcaatgg
     1741 cacaggccat aaccctgctg gcggacgtgg aacggttatg tccgcaactt gtgaaaacgc
     1801 cgccgggtgg tctgttacaa tctgttgatt tacatccggc gataaaagcc ctgaaagatg
     1861 agtgatctag agtcgacctg catctagaga aagaggagaa atactagatg atggccagca
     1921 gaggcgtaaa caaggttatt ctcgttggta atctgggtca ggacccggaa gtacgctaca
     1981 tgccaaatgg tggcgcagtt gccaacatta cgctggctac ttccgaatcc tggcgtgata
     2041 aagcgaccgg cgagatgaaa gagcagactg aatggcaccg cgttgtgctg ttcggcaaac
     2101 tggcagaagt ggccagcgaa tatctgcgta aaggttctca ggtttatatc gaaggtcagc
     2161 tgcgtacccg taaatggacc gatcaatccg gtcaggatcg ctacaccaca gaagtcgtgg
     2221 tgaacgttgg cggcaccatg cagatgctgg gtggtcgtca gggtggtggc gctccggcag
     2281 gtggtaatat cggtggtggt cagccgcagg gcggttgggg tcagcctcag cagccgcagg
     2341 gtggcaatca gttcagcggc ggcgcgcagt ctcgcccgca gcagtccgct ccggcagcgc
     2401 cgtctaacga gccgccgatg gactttgatg atgacattcc gttctgaagc ttgcggccgc
     2461 gtcgtgactg ggaaaaccct ggcgactagt cttggactcc tgttgataga tccagtaatg
     2521 acctcagaac tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatt
     2581 ggtgagaatc caggggtccc caataattac gatttaaatt tgtgtctcaa aatctctgat
     2641 gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct gcttacataa
     2701 acagtaatac aaggggtgtt atgagccata ttcagcgtga aacgagctgt agccgtccgc
     2761 gtctgaacag caacatggat gcggatctgt atggctataa atgggcgcgt gataacgtgg
     2821 gtcagagcgg cgcgaccatt tatcgtctgt atggcaaacc ggatgcgccg gaactgtttc
     2881 tgaaacatgg caaaggcagc gtggcgaacg atgtgaccga tgaaatggtg cgtctgaact
     2941 ggctgaccga atttatgccg ctgccgacca ttaaacattt tattcgcacc ccggatgatg
     3001 cgtggctgct gaccaccgcg attccgggca aaaccgcgtt tcaggtgctg gaagaatatc
     3061 cggatagcgg cgaaaacatt gtggatgcgc tggccgtgtt tctgcgtcgt ctgcatagca
     3121 ttccggtgtg caactgcccg tttaacagcg atcgtgtgtt tcgtctggcc caggcgcaga
     3181 gccgtatgaa caacggcctg gtggatgcga gcgattttga tgatgaacgt aacggctggc
     3241 cggtggaaca ggtgtggaaa gaaatgcata aactgctgcc gtttagcccg gatagcgtgg
     3301 tgacccacgg cgattttagc ctggataacc tgattttcga tgaaggcaaa ctgattggct
     3361 gcattgatgt gggccgtgtg ggcattgcgg atcgttatca ggatctggcc attctgtgga
     3421 actgcctggg cgaatttagc ccgagcctgc aaaaacgtct gtttcagaaa tatggcattg
     3481 ataatccgga tatgaacaaa ctgcaatttc atctgatgct ggatgaattt ttctaataat
     3541 taattggacc gcggtccgcg cgttgtcctt ttccgctgca taaccctgct tcggggtcat
     3601 tatagcgatt ttttcggtat atccatcctt tttcgcacga tatacaggat tttgccaaag
     3661 ggttcgtgta gactttcctt ggtgtatcca acggcgtcag ccgggcagga taggtgaagt
     3721 aggcccaccc gcgagcgggt gttccttctt cactgtccct tattcgcacc tggcggtgct
     3781 caacgggaat cctgctctgc gaggctggcc gtaggccggc ctcagcctgc cgccttgggc
     3841 cgggtgatgt cgtacttgcc cgccgcgaac tcggttaccg tccagcccag cgcgaccagc
     3901 tccggcaacg cctcgcgcac ccgctggcgg cgcttgcgca tggtcgaacc actggcctct
     3961 gacggccaga catagccgca caaggtatct atggaagcct tgccggtttt gccggggtcg
     4021 atccagccac acagccgctg gtgcagcagg cgggcggttt cgctgtccag cgcccgcacc
     4081 tcgtccatgc tgatgcgcac atgctggccg ccacccatga cggcctgcgc gatcaagggg
     4141 ttcagggcca cgtacaggcg cccgtccgcc tcgtcgctgg cgtactccga cagcagccga
     4201 aacccctgcc gcttgcggcc attctgggcg atgatggata ccttccaaag gcgctcgatg
     4261 cagtcctgta tgtgcttgag cgccccacca ctatcgacct ctgccccgat ttcctttgcc
     4321 agcgcccgat agctaccttt gaccaccatg gcatcagcgg tgacggcctc ccacttgggt
     4381 tccaggaaca gccggagctg ccgtccgcct tcggtcttgg gttccgggcc aagcactagg
     4441 ccattaggcc cagccatggc caccagccct tgcaggatgc gcagatcatc agcgcccagc
     4501 ggctccgggc cgctgaactc gatccgcttg ccgtcgccgt agtcatacgt cacgtccagc
     4561 ttgctgcgct tgcgctcgcc ccgcttgagg gcacggaaca ggccgggggc cagacagtgc
     4621 gccgggtcgt gccggacgtg gctgaggctg tgcttgttct taggcttcac cacggggcac
     4681 ccccttgctc ttgcgctgcc tctccagcac ggcgggcttg agcaccccgc cgtcatgccg
     4741 cctgaaccac cgatcagcga acggtgcgcc atagttggcc ttgctcacac cgaagcggac
     4801 gaagaaccgg cgctggtcgt cgtccacacc ccattcctcg gcctcggcgc tggtcatgct
     4861 cgacaggtag gactgccagc ggatgttatc gaccagtacc gagctgcccc ggctggcctg
     4921 ctgctggtcg cctgcgccca tcatggccgc gcccttgctg gcatggtgca ggaacacgat
     4981 agagcacccg gtatcggcgg cgatggcctc catgcgaccg atgacctggg ccatggggcc
     5041 gctggcgttt tcttcctcga tgtggaaccg gcgcagcgtg tccagcacca tcaggcggcg
     5101 gccctcggcg gcgcgcttga ggccgtcgaa ccactccggg gccatgatgt tgggcaggct
     5161 gccgatcagc ggctggatca gcaggccgtc agccacggct tgccgttcct cggcgctgag
     5221 gtgcgcccca agggcgtgca ggcggtgatg aatggcggtg ggcgggtctt cggcgggcag
     5281 gtagatcacc gggccggtgg gcagttcgcc cacctccagc agatccggcc cgcctgcaat
     5341 ctgtgcggcc agttgcaggg ccagcatgga tttaccggca ccaccgggcg acaccagcgc
     5401 cccgaccgta ccggccacca tgttgggcaa aacgtagtcc agcggtggcg gcgctgctgc
     5461 gaacgcctcc agaatattga taggcttatg ggtagccatt gattgcctcc tttgcaggca
     5521 gttggtggtt aggcgctggc ggggtcacta cccccgccct gcgccgctct gagttcttcc
     5581 aggcactcgc gcagcgcctc gtattcgtcg tcggtcagcc agaacttgcg ctgacgcatc
     5641 cctttggcct tcatgcgctc ggcatatcgc gcttggcgta cagcgtcagg gctggccagc
     5701 aggtcgccgg tctgcttgtc cttttggtct ttcatatcag tcaccgagaa acttgccggg
     5761 gccgaaaggc ttgtcttcgc ggaacaagga caaggtgcag ccgtcaaggt taaggctggc
     5821 catatcagcg actgaaaagc ggccagcctc ggccttgttt gacgtataac caaagccacc
     5881 gggcaaccaa tagcccttgt cacttttgat caggtagacc gaccctgaag cgcttttttc
     5941 gtattccata aaaccccctt ctgtgcgtga gtactcatag tataacaggc gtgagtacca
     6001 acgcaagcac tacatgctga aatctggccc gcccctgtcc atgcctcgct ggcggggtgc
     6061 cggtgcccgt gccagctcgg cccgcgcaag ctggacgctg ggcagaccca tgaccttgct
     6121 gacggtgcgc tcgatgtaat ccgcttcgtg gccgggcttg cgctctgcca gcgctgggct
     6181 ggcctcggcc atggccttgc cgatttcctc ggcactgcgg ccccggctgg ccagcttctg
     6241 cgcggcgata aagtcgcact tgctgaggtc atcaccgaag cgcttgacca gcccggccat
     6301 ctcgctgcgg tactcgtcca gcgccgtgcg ccggtggcgg ctaagctgcc gctcgggcag
     6361 ttcgaggctg gccagcctgc gggccttctc ctgctgccgc tgggcctgct cgatctgctg
     6421 gccagcctgc tgcaccagcg ccgggccagc ggtggcggtc ttgcccttgg attcacgcag
     6481 cagcacccac ggctgataac cggcgcgggt ggtgtgcttg tccttgcggt tggtgaagcc
     6541 cgccaagcgg ccatagtggc ggctgtcggc gctggccggg tcggcgtcgt actcgctggc
     6601 cagcgtccgg gcaatctgcc cccgaagttc accgcctgcg gcgtcggcca ccttgaccca
     6661 tgcctgatag ttcttcgggc tggtttccac taccagggca ggctcccggc cctcggcttt
     6721 catgtcatcc aggtcaaact cgctgaggtc gtccaccagc accagaccat gccgctcctg
     6781 ctcggcgggc ctgatataca cgtcattgcc ctgggcattc atccgcttga gccatggcgt
     6841 gttctggagc acttcggcgg ctgaccattc ccggttcatc atctggccgg tggtggcgtc
     6901 cctgacgccg atatcgaagc gctcacagcc catggccttg agctgtcggc ctatggcctg
     6961 caaagtcctg tcgttcttca tcgggccacc aagcgattcc cacacattat acgagccgga
     7021 agcataaagt gtagctagat ccgaaggatg agccgggctg aatgatcgac cgagacaggc
     7081 cctgcggggc tgcacacgcg cccccaccct tcgggtaggg ggaaaggccg ctaaagcggc
     7141 taaaagcgct ccagcgtatt tctgcggggt ttggtgtggg gtttagcggg ctttgcccgc
     7201 ctttccccct gccgcgcagc ggtggggcgg tgtgtagcct agcgcagcga atagaccagc
     7261 tatccggcct ctggccgggc atattgggca agggcagcag cgccccacaa gggcgctgat
     7321 aaccgcgcct agtggattat tcttagataa tcatggatgg atttttccaa caccccgcca
     7381 gcccccgccc ctgctgggtt tgcaggtttg ggggcgtgac agttattgca ggggttcgtg
     7441 acagttattg caggggggcg tgacagttat tgcaggggtt cgtgacagtt agggcgcgcc
     7501 cagctgtcta gggcggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga
     7561 taaaacgaaa ggcccagtct ttcgactgag cctttcgttt tatttgatgc ctttaattaa
     7621 acgttcgtaa tcaagccact tcctttttgc attgacgcag ggtgtcggaa ggcaactcgc
     7681 cgaacgcgct cctatagttt tcagcgaagc gtcccaaatg taagaagccg tagtctaggg
     7741 ctatctcagt tatactacgc acattggcac tgggatcgtt caagcaggcg cggatgcttt
     7801 cgagcttgcg gttgcggatg tagttcttcg gcgtggtgcc ggcgtgcttc tcgaacaaat
     7861 tgtagagcga gcgtggactc atcatcgcca gctccgctaa ccgctcaagg ctgatattcc
     7921 gtttgagatt ctcctcaatg aattgaacga ctcgctcgaa agacgggtta cctttgctga
     7981 aaatttcacg gctgacattg ctgcccagca tttcgagcag cttggaagcg atgatccccg
     8041 catagtgctc ttggacccga ggcatcgact ttgtatgttc cgcttcgtca caaactaacc
     8101 cgagtagatt gataaagcca tcgagttgct ggagattgtg tcgcgcggcg aaacggatac
     8161 cctccctcgg cttgtgccaa ttgttgtcac tgcacgcccg atcaaggacc actgagggca
     8221 atttaacgat aaatttctcg caatcttctg aataggtcag gtcggcttgg tcatccggat
     8281 tgagcagcaa tagttcgccc ggcgcaaaat agtgctcctg gccatggcca cgccacaggc
     8341 aatggccttt gagtattatt tgcagatgat aacaggtctc taatccaggc gagattaccc
     8401 tcacgctacc gccgtagctg attcgacaca gatcgaggca tccgaagatt ctgtggtgca
     8461 gcctgcctgc cgggcgcccg cccttgggca ggcgaataga gtgcgtaccg acatactggt
     8521 taacataatc ggagactgca tagggctcgg cgtggacgaa gatctgactt ttctcgttca
     8581 ataagcaaaa atccatagtt cacggttctc ttattttaat gtgggctgct tggtgtgatg
     8641 tagaaaggcg ccaagtcgat gaaaatgcat ctcgacgtga tgcgtatacg ggttaccccc
     8701 attgccacgt tgcgccatcc tttttgcaat cagtgaccac ttttccaagc aaaaataacg
     8761 ccaagcagaa cgaagacgtt ctttttaaga agcgagaaca ccagaagttc gtgctgtcgg
     8821 ggcatggggc gacgaattgg cggataaagg ggatctgctg gatattacgg cctttttaaa
     8881 gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct
     8941 gatgaatgct catccgtaat tacgtatggc aatgaaagac ggtgagctgg tgatatggga
     9001 tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg
     9061 gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg
     9121 ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc
     9181 agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt
     9241 cttcgccccc gttttcacca tgcatgggaa ttagcttgat ctgaccaacg accggtagcg
     9301 gagctatcca acggcggtat accaggaaaa cacacagcag gtacatcaga acagtaccat
     9361 gactgaagaa caaatagttt tttcctgatc cataaagcag aacggcctgc tccatgacaa
     9421 atctggctcc ccaactaatg ccccatgcag ccagcataac cagcataaag tgcagtgtcc
     9481 ggtttgatag ggataagtcc agccttgcaa gaagcggata caggagtgca aaaaatggct
     9541 atctctagta aggcctaccc cttaggcttt atgcaacaga aacaataata atggagtcat
     9601 gaccatgcct aggccgcggg ccgcgcgaat tcagaaggag aatataccat gaaccaaatc
     9661 gtgaagttca ctgacgactc tggcctggcg gttcaagtta ctccagacga tgttcgccgt
     9721 tatatctgtg agaacgctac tgaaaaagag gtgggcctct ttctgcaact ctgtcagact
     9781 caacgtctga atccgtttgt gaaagacgct tacctggtga aatacggcgg tgctccagct
     9841 tctatgatta cttcctatca agtttttaac cgtcgcgcgt gtcgtgatgc taactatgat
     9901 ggtatcaaat ctggtgtggt tgttctgcgt gacggtgatg ttgtgcataa acgtggtgct
     9961 gcgtgctaca aaaaggcggg tgaggagctc atcggtggtt gggcggaagt tcgctttaag
    10021 gatggccgcg agactgcgta tgctgaggtg gcgctcgacg actattccac cggcaaatct
    10081 aattgggcga aaatgccggg tgttatgatc gaaaaatgcg cgaaggctgc tgcttggcgc
    10141 ctcgcgttcc cggacacttt tcagggcatg tacgctgcgg aggaaatgga tcaagcgcaa
    10201 cagccagaac aggtgcgcgc tcaggcggag caaccagtgg atctccagcc aatccgcgaa
    10261 ctcttcaagc catattgcga acacttcggc atcactccgg ctgagggtat gactgctgtt
    10321 tgtggtgcgg tgggcgctga aggcatgcac tctatgaccg agcagcaagc tcgccgtgct
    10381 cgcgcttgga tggaggaaga aatggctgcg ccagctgtgg aagcggagta tgaggttgtt
    10441 gacgagggcg aggtgtttta ag
//
