LOCUS       sequence-455320-        5790 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Entry Clone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5790)
  AUTHORS   Calderwood MA, Venkatesan K, Xing L, Chase MR, Vazquez A, Holthaus 
            AM, Ewence AE, Li N, Hirozane-Kishikawa T, Hill DE, Vidal M, Kieff 
            E, Johannsen E
  TITLE     Epstein-Barr virus and virus human protein interaction maps.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 May 1;104(18):7606-11. doi: 
            10.1073/pnas.0702332104. Epub 2007 Apr 19.
  PUBMED    17446270
REFERENCE   2  (bases 1 to 5790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0702332104"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2007-05-1- 1"; volume: "104"; issue: "18"; pages: "7606-11"
COMMENT     Sequence Label: pENTR223-BPLF1_2001-3000
FEATURES             Location/Qualifiers
     source          1..5790
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3669..3768)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3785..3804)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3786..3804)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3809..3825)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3809..3825)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4256..5047
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5140..5728
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5629..5648
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcatg cgggcacaat ttctggcggc ggcagggccg gcgaaatacg ccggcaccct
      721 ctggctggaa acagagtctc cctgcgaccc ccttaacccg gcctacgtct ccgcagacac
      781 ccaggagccg ctcaactaca tccccgtcta ccacaacttt ctggaatatg tcatgcccac
      841 cgtgctggag aacccagagg ccttttccct gaccccagcc ggacgccccc aagctattgg
      901 tcccccgcag gatgaccaag agaggaggaa aagaaccctg gccagtgtag cttccgcccg
      961 cctcagcgcc gccgccgccg actcctactg ggacacctgg cccgacgtgg agtccaacgc
     1021 cggggagctg cttcgggaat acgtctctgc gcccaaagcc ctgatggaag acctggcaga
     1081 caacccaatt gtggccatga cgcttctagc gcacgccagt ctcatcgcct ctaggaatca
     1141 ccccccttat ccagccccgg ccacagaccg ggaagttatt ctattggaac aacgggagat
     1201 gatggcgctg ctggtcggca cacatccggc ctacgctgcc gccttcctgg gcgccccatc
     1261 gttctacgca ggactcggcc tggtctcggc tctagcgaga gatgggggtt tgggagacct
     1321 cctgtcggac tcggtcctca cctacaggct ggtcaggagt ccagcctccg ggaggggggg
     1381 catgccctca accacccgcg gcagcaacga cggggaagac gcgcgccgcc tcacccgcca
     1441 ccgcatcgcc ggtccaccca ccggcttcat cttcttccag gacgcctggg aggagatgga
     1501 tacccgggcc gccctctggc cgcaccccga gttcttgggc ctggtccaca accaaagtac
     1561 ggcccgcgcc cgagcctgca tgctgctcct cgcacgccgg tgcttcgccc ccgaggccct
     1621 tcagcagctc tggcactcat tgcgcccgct ggagggcccg gtcgcatttc aggactacct
     1681 gcgcgacttt gtgaagcagg cctatacacg cggagaagag ctccccaggg cggagggact
     1741 cgaggtcccc cgcgaaaccc cgtcttccta cggcaccgtt accggccggg ccctgagaaa
     1801 tcttatgcct tatggcactc ccattaccgg tcctaagagg ggctccggtg acaccatccc
     1861 cgtctccgtt tttgaggcgg ccgtggcggc ggccttcctc ggtcggcccc taactctctt
     1921 tgtctcctct caatatctgt ttaacctgaa gaccctggga caggtcaggg tcgttgctcc
     1981 cctcctctac tgcgacgggc actctgagcc cttccggtcc ctggtggaga ccatctcgct
     2041 gaattttctg caggacctgg acggctactc cgagtctttc gagccagaga tgtccatctt
     2101 tgcacgccag gccgtgtggc tacgcgagct tctcaccgag gccagggccg ccaaacctaa
     2161 ggaggccagg ccccccaccg tggccatcct ggccaacaga aaaaacatca tctggaagtg
     2221 tttcacctat cggcacaatc tgccggacgt gcagttctac tttaacgcgg ccggggcctc
     2281 acgatggccg accgatgtac tcaacccctc cttctacgag cacgaagatc ctccactgcc
     2341 ggttggatac cagcttcccc cgaatccacg caacgtccag gagctgttct ccggtttccc
     2401 cccaagggtg gggcacggac tggtcagcgg ggacgggttt cagtcagcag ataacacccc
     2461 ggcctcctcc gaccggctcc agcagttggg aggaggggag acggaccagg gggaaaaggg
     2521 gagcactact gctgaatctg aggcctctgg ccctcccagc ccacagagcc ccctcttaga
     2581 gaaggtggcc cctggcaggc ccagggactg gctgtctcct acctcctccc cccgggacgt
     2641 gacagttacc ccgggattgg ccgcccccat cactctccca ggcccccgat tgatggcaag
     2701 gccctatttc ggggccgaaa cgagggcttc cgagagccca gaccggtctc cgggaagctc
     2761 cccaaggcca tggcccaaag attccctgga gctccttccc caaccagcac cacaacagcc
     2821 cccctcaagt ccctgggctt ctgaacaagg gcccatcgtc tacacattgt ctccacactc
     2881 tacaccatcg actgcctcgg ggtcacagaa gaaacatacc atccagattc cagggttggt
     2941 gccttcacag aagcccagtt atccaccctc agccccctac aagcccggcc agtcgacggg
     3001 aggcatagct cccacaccat cagcggcatc tttaacgact tttgggctcc agccccagga
     3061 cacacaagcc tcctctcagg acccacccta tggccattct atcatgcaac gggagaagaa
     3121 acagcagggg ggccgggaag aagccgcgga gattcggccc tccgccaccc gcctccccac
     3181 ggccgtcggg ctgcgacccc gtgcgcccgt ggtggccgcc ggcgcagcag cctccgctac
     3241 tccagcgttc gacccgggag aagcgccgtc cggctttccc atcccccagg cacccgccct
     3301 ggggtccggc ttggccgccc cggcgcacac cccagttggt gcattggcac cgcgccccca
     3361 aaaaacgcaa gcacaaaggc cccaagatgc agctgccctg cccaccccca caattaaagc
     3421 ggtgggtgcc aggcccgtgc caaaggccac gggggcgctg gcggccggcg cccgtcctcg
     3481 ggggcagccc accgcggccc cgccgtcggc tgcatctccg ccgcgcgtgt ctctccctgt
     3541 caggagcaga cagcaacaat cccccgcaat cccattgccc ccaatgcatt ccggctcgga
     3601 gcctggcgcg cggcccgagg tgcgcctatc ccagtaccgg catgcgggtc cccaaacata
     3661 caccgtgcga ccaactttct tgtacaaagt tggcattata agaaagcatt gcttatcaat
     3721 ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgcc atccagctga
     3781 tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc tctggcccgt
     3841 gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat gcctcctcta
     3901 gaccagccag gacagaaatg cctcgacttc gctgctaccc aaggttgccg ggtgacgcac
     3961 accgtggaaa cggatgaagg cacgaaccca gtggacataa gcctgttcgg ttcgtaagct
     4021 gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg aacgcagcgg
     4081 tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg gggtacagtc
     4141 tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg
     4201 gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa acattatgag
     4261 ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg tcatcgagcg
     4321 ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg atggcggcct
     4381 gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg atgaaacaac
     4441 gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag agagcgagat
     4501 tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt ggcgttatcc
     4561 agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg caggtatctt
     4621 cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa gagaacatag
     4681 cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg aacaggatct
     4741 atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact gggctggcga
     4801 tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa ccggcaaaat
     4861 cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc agtatcagcc
     4921 cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct tggcctcgcg
     4981 cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg
     5041 caaataaccc tcgagccacc catgaccaaa atcccttaac gtgagttacg cgtcgttcca
     5101 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     5161 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     5221 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     5281 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     5341 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     5401 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     5461 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     5521 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     5581 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     5641 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     5701 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     5761 ggccttttgc tggccttttg ctcacatgtt
//
