LOCUS       sequence-455426-       10249 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid encodes SgRNA, SpCas9 and homologous arms for homologous 
            recombination of mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10249)
  AUTHORS   Wiull K, Haugen LK, Eijsink VGH, Mathiesen G
  TITLE     CRISPR/Cas9-mediated genomic insertion of functional genes into 
            Lactiplantibacillus plantarum WCFS1.
  JOURNAL   Microbiol Spectr. 2025 Feb 4;13(2):e0202524. doi: 
            10.1128/spectrum.02025-24. Epub 2025 Jan 16.
  PUBMED    39817779
REFERENCE   2  (bases 1 to 10249)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microbiol 
            Spectr."; date: "2025-02-4"; volume: "13(2):e0202524. doi"; pages: "
            10.1128/spectrum.02025-24. Epub 2025 Jan 16"
COMMENT     Sequence Label: pCRISPR-PsppA-mCherry
FEATURES             Location/Qualifiers
     source          1..10249
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3221..7327
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             3221..7324
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     rep_origin      7652..8240
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8141..8160
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      10076..10147
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      10163..10190
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 cccctggtat aataactata ataaacgact tcggtggaat gtttaagagc tatgctggaa
       61 acagcatagc aagtttaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt
      121 cggtgctttt tttgaagctt gggcccgaac aaaaactcat ggatccctcc agtaactcga
      181 gctacaagat ccaccaactt tattgcctaa aatgctggcg atgcttcaaa ccggtgacta
      241 tgactgtatc ggaacaatgc gggaaaatcg tgtcggtgaa ccaatgattc ggtcattctt
      301 atcacgctcg ttttactcag ttgtcaacaa aatttcaaaa gtacaaatta ttccaaacgc
      361 ccgtgattac cggttaatga cacgtcagat ggttgatgcc atcttggaat tgcccgaata
      421 taaccgattt tcaaaaggca tcttcaactg ggtcggtttc cgaacgactt atttgaagtt
      481 tgagaatcag ccccgctccg ctggcgaaac acactggtca atttggcagc tgttcaatta
      541 ttcaatggac gctatcgtcg acttttcaga tgtacccctg aagattgcga ccttcaccgg
      601 tggcttgctc tcactaatct caattatcag tatatttgtc gttattattc gtaaaatatt
      661 atttgataac agcgtcagtg gttgggcttc aaccgtcaca attttgctct taatcggcgg
      721 catccagctg ttctgtctgg ggattatcgg taattatatt ggtaaaattt atatcgaatc
      781 aaaacaccgg ccccactaca ttgtgcagga aaagaagtaa acttttcaac ctaaactgag
      841 acgcatacga ccatatcggg aaaatggtat ggtcgtgtgc gtttcggcat ctggatgaag
      901 ttaaaggtta tgaatgaaat tattatttaa aagctctttt gggggcatca gtccttgtca
      961 agttgatgaa tgaaataata tgatttttaa ttacttttca aacggcttca tcgcggtatt
     1021 caaatgcggt agagccgttt ctttgtacta ttcgtatgat ttcattaaag tattaaaagc
     1081 ctggttaaag gtatcaacca ccgcctcgct ggttggctga cttaactaaa gatgttttag
     1141 agcgccgtaa atcgggcttt gtgcttgtta ttagcccata agaagagcac cggtgggccc
     1201 atattaacgt ttaaccgata aagttgaacg ttaatatttt ttttgcgcag aaatggtaaa
     1261 ttgaagcata atagtcttgt aaggtattta gctggctggc gtaaagtatg ctttataaaa
     1321 taatatatag gagtatgatt catatgagca aaggagaaga agataacatg gcaatcatca
     1381 aagaatttat gcgtttcaaa gttcacatgg aaggttctgt aaacggacac gaatttgaaa
     1441 ttgaaggtga aggtgaaggc cgtccttatg aaggaacaca aacggcaaag ctgaaagtaa
     1501 caaaaggcgg accgcttccg tttgcatggg atatcctttc tccgcaattc atgtacggtt
     1561 caaaagcata cgtgaagcat ccggctgata ttcctgatta tttgaagctg tcattccctg
     1621 aaggcttcaa atgggagcgt gtgatgaact ttgaagatgg cggtgttgtt actgttactc
     1681 aagattcaag ccttcaagac ggtgaattta tttacaaagt gaagctgcgc ggaacaaact
     1741 tcccatctga cggacctgtc atgcaaaaga aaacaatggg ctgggaagca agctctgaac
     1801 gcatgtatcc agaggacggt gctttaaaag gagaaatcaa acagcgtttg aagctgaaag
     1861 acggcggaca ctatgacgct gaagtgaaaa caacttacaa agcgaaaaag ccggttcagc
     1921 ttccaggtgc ttacaacgta aacatcaaac ttgatattac aagccacaat gaagattata
     1981 cgattgttga acaatatgaa cgcgctgaag gccgtcattc aactggcgga atggatgagc
     2041 tttacaaata aaagcttcgc tcttcataat cctacttggt tagactgagt tacttgattt
     2101 aatttcttac ttagtttgtt tgatgcgttg aggccgctta atacaacttt aacattttag
     2161 atggagtaca gatacagata tcaagtgttt aactgaaaac ccaaaaccaa tcgattcatg
     2221 atgaagccac ttattataga ctgagcgcga caaacctggc ttgctaaaca aggtggcctg
     2281 aacaccatga atatccgagc cgacggtttt gattccttga taggctaatt tatgttgtgc
     2341 attcaatctt tgtgctgtat tttttggact tttttaaaaa gaagtcaaac ctcctgctcc
     2401 ttgatttgaa tcttaagttg acgtttcttg agccttagtc gttcaacctc agtcattttt
     2461 tcaattggtt tcgtccatcc aagccggtct ttaagtgcga tgtaaccatt ggtggtggtt
     2521 ttgagcatcc agaaacggat ttgctgataa gatacattgt aatgtttcgc tgctcgggaa
     2581 tatgcatgtt cactcaccgt aatgtattca acgattctaa tgcgttcttc aaaagttatt
     2641 tttctactca taatgtggac cagcaattca ctgatccggt cctccagccg tctagtactt
     2701 ctaaggccaa atctgatttg aatatcccac aaactacttt catcattcaa gtacgctgat
     2761 atggcctgcc gcttaatctc tgagatatat cgaatccaat tgtgctgttc ttttaatcct
     2821 tccagaccat cctgcatata aaaattttga catctgatga aagtacttcg agcgatacca
     2881 cacgctttaa aacacgttga tttagtcagg ttagtttttt caagacccag aataatagct
     2941 aatttttctg tgaccataaa attggttcta gatataataa aatctcctta aaattgatag
     3001 atgaagtata tcatttcatc tatcaacctt aaggggacta tagcacttaa aactgtctgg
     3061 ctcgtaccgg tgggcccata ttaacgttta accgataaag ttgaacgtta atattttttt
     3121 tgcgcagaaa tggtaaattg aagcataata gtcttgtaag gtatttagct ggctggcgta
     3181 aagtatgctt tataaaataa tatataggag tatgattccc atggataaga aatactcaat
     3241 aggcttagat atcggcacaa atagcgtcgg atgggcggtg atcactgatg aatataaggt
     3301 tccgtctaaa aagttcaagg ttctgggaaa tacagaccgc cacagtatca aaaaaaatct
     3361 tataggggct cttttatttg acagtggaga gacagcggaa gcgactcgtc tcaaacggac
     3421 agctcgtaga aggtatacac gtcggaagaa tcgtatttgt tatctacagg agattttttc
     3481 aaatgagatg gcgaaagtag atgatagttt ctttcatcga cttgaagagt cttttttggt
     3541 ggaagaagac aagaagcatg aacgtcatcc tatttttgga aatatagtag atgaagttgc
     3601 ttatcatgag aaatatccaa ctatctatca tctgcgaaaa aaattggtag attctactga
     3661 taaagcggat ttgcgcttaa tctatttggc cttagcgcat atgattaagt ttcgtggtca
     3721 ttttttgatt gagggagatt taaatcctga taatagtgat gtggacaaac tatttatcca
     3781 gttggtacaa acctacaatc aattatttga agaaaaccct attaacgcaa gtggagtaga
     3841 tgctaaagcg attctttctg cacgattgag taaatcaaga cgattagaaa atctcattgc
     3901 tcagctcccc ggtgagaaga aaaatggctt atttgggaat ctcattgctt tgtcattggg
     3961 tttgacccct aattttaaat caaattttga tttggcagaa gatgctaaat tacagctttc
     4021 aaaagatact tacgatgatg atttagataa tttattggcg caaattggag atcaatatgc
     4081 tgatttgttt ttggcagcta agaatttatc agatgctatt ttactttcag atatcctaag
     4141 agtaaatact gaaataacta aggctcccct atcagcttca atgattaaac gctacgatga
     4201 acatcatcaa gacttgactc ttttaaaagc tttagttcga caacaacttc cagaaaagta
     4261 taaagaaatc ttttttgatc aatcaaaaaa cggatatgca ggttatattg atgggggagc
     4321 tagccaagaa gaattttata aatttatcaa accaatttta gaaaaaatgg atggtactga
     4381 ggaattattg gtgaaactaa atcgtgaaga tttgctgcgc aagcaacgga cctttgacaa
     4441 cggctctatt ccccatcaaa ttcacttggg tgagctgcat gctattttga gaagacaaga
     4501 agacttttat ccatttttaa aagacaatcg tgagaagatt gaaaaaatct tgacttttcg
     4561 aattccttat tatgttggtc cattggcgcg tggcaatagt cgttttgcat ggatgactcg
     4621 gaagtctgaa gaaacaatta ccccatggaa ttttgaagaa gttgtcgata aaggtgcttc
     4681 agctcaatca tttattgaac gcatgacaaa ctttgataaa aatcttccaa atgaaaaagt
     4741 actaccaaaa catagtttgc tttatgagta ttttacggtt tataacgaat tgacaaaggt
     4801 caaatatgtt actgaaggaa tgcgaaaacc agcatttctt tcaggtgaac agaagaaagc
     4861 cattgttgat ttactcttca aaacaaatcg aaaagtaacc gttaagcaat taaaagaaga
     4921 ttatttcaaa aaaatagaat gttttgatag tgttgaaatt tcaggagttg aagatagatt
     4981 taatgcttca ttaggtacct accatgattt gctaaaaatt attaaagata aagatttttt
     5041 ggataatgaa gaaaatgaag atatcttaga ggatattgtt ttaacattga ccttatttga
     5101 agatagggag atgattgagg aaagacttaa aacatatgct cacctctttg atgataaggt
     5161 gatgaaacag cttaaacgtc gccgttatac tggttgggga cgtttgtctc gaaaattgat
     5221 taatggtatt agggataagc aatctggcaa aacaatatta gattttttga aatcagatgg
     5281 ttttgccaat cgcaatttta tgcagctgat ccatgatgat agtttgacat ttaaagaaga
     5341 cattcaaaaa gcacaagtgt ctggacaagg cgatagttta catgaacata ttgcaaattt
     5401 agctggtagc cctgctatta aaaaaggtat tttacagact gtaaaagttg ttgatgaatt
     5461 ggtcaaagta atggggcggc ataagccaga aaatatcgtt attgaaatgg cacgtgaaaa
     5521 tcagacaact caaaagggcc agaaaaattc gcgagagcgt atgaaacgaa tcgaagaagg
     5581 tatcaaagaa ttaggaagtc agattcttaa agagcatcct gttgaaaata ctcaattgca
     5641 aaatgaaaag ctctatctct attatctcca aaatggaaga gacatgtatg tggaccaaga
     5701 attagatatt aatcgtttaa gtgattatga tgtcgatcac attgttccac aaagtttcct
     5761 taaagacgat tcaatagaca ataaggtctt aacgcgttct gataaaaatc gtggtaaatc
     5821 ggataacgtt ccaagtgaag aagtagtcaa aaagatgaaa aactattgga gacaacttct
     5881 aaacgccaag ttaatcactc aacgtaagtt tgataattta acgaaagctg aacgtggagg
     5941 tttgagtgaa cttgataaag ctggttttat caaacgccaa ttggttgaaa ctcgccaaat
     6001 cactaagcat gtggcacaaa ttttggatag tcgcatgaat actaaatacg atgaaaatga
     6061 taaacttatt cgagaggtta aagtgattac cttaaaatct aaattagttt ctgacttccg
     6121 aaaagatttc caattctata aagtacgtga gattaacaat taccatcatg cccatgatgc
     6181 gtatctaaat gccgtcgttg gaactgcttt gattaagaaa tatccaaaac ttgaatcgga
     6241 gtttgtctat ggtgattata aagtttatga tgttcgtaaa atgattgcta agtctgagca
     6301 agaaataggc aaagcaaccg caaaatattt cttttactct aatatcatga acttcttcaa
     6361 aacagaaatt acacttgcaa atggagagat tcgcaaacgc cctctaatcg aaactaatgg
     6421 ggaaactgga gaaattgtct gggataaagg gcgagatttt gccacagtgc gcaaagtatt
     6481 gtccatgccc caagtcaata ttgtcaagaa aacagaagta cagacaggcg gattctccaa
     6541 ggagtcaatt ttaccaaaaa gaaattcgga caagcttatt gctcgtaaaa aagactggga
     6601 tccaaaaaaa tatggtggtt ttgatagtcc aacggtagct tattcagtcc tagtggttgc
     6661 taaggtggaa aaagggaaat cgaagaagtt aaaatccgtt aaagagttac tagggatcac
     6721 aattatggaa agaagttcct ttgaaaaaaa tccgattgac tttttagaag ctaaaggata
     6781 taaggaagtt aaaaaagact taatcattaa actacctaaa tatagtcttt ttgagttaga
     6841 aaacggtcgt aaacggatgc tggctagtgc cggagaatta caaaaaggaa atgagctggc
     6901 tctgccaagc aaatatgtga attttttata tttagctagt cattatgaaa agttgaaggg
     6961 tagtccagaa gataacgaac aaaaacaatt gtttgtggag cagcataagc attatttaga
     7021 tgagattatt gagcaaatca gtgaattttc taagcgtgtt attttagcag atgccaattt
     7081 agataaagtt cttagtgcat ataacaaaca tagagacaaa ccaatacgtg aacaagcaga
     7141 aaatattatt catttattta cgttgacgaa tcttggagct cccgctgctt ttaaatattt
     7201 tgatacaaca attgatcgta aacgatatac gtctacaaaa gaagttttag atgccactct
     7261 tatccatcaa tccatcactg gtctttatga aacacgcatt gatttgagtc agctaggagg
     7321 tgactgaccc gggttcgaag gcgccaagct tcaaattaca gcacgtgttg ctttgattga
     7381 tagccaaaaa gcagcagttg ataaagcaat tactgatatt gctgaaaaat tgtaatttat
     7441 aaataaaaat caccttttag aggtggtttt tttatttata aattattcgt ttgatttcgc
     7501 tttcgataga acaatcaaag cgagaataag gaagataaat cccataaggg cgggagcaga
     7561 atgtccgaga ctaattcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     7621 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     7681 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     7741 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     7801 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     7861 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     7921 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     7981 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     8041 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     8101 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     8161 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     8221 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     8281 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     8341 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgctgcgagt
     8401 cagtgagcga gaaggattat tcggctggtt gagacgttaa aatgataaag gttgtattaa
     8461 tcttatatta cggttataat gtactcaact taataaatga acgcaaaaaa aagaaccctc
     8521 aacttagcag agttaggatt cacgacttat cagcacaacc tgataagatt ttcgatagca
     8581 agtactacca atacaagcta tctaacttgg tactattata acatgtaggc taagtttttc
     8641 aaccattgat acttaaagta aacggttgtt atcgggaatc ttaacagaaa cctgatagca
     8701 accgtttttt tgttattcaa tggttagcaa ccatcaaagc aactaaaggc tggaaacctg
     8761 ttcttagcta gtaaaacctc ccgtgagtgt cgttcgtgac cccgcttgca gttaacaaca
     8821 taggtatgct aaaccttgtc gagatcaacg cgactaaaga cgtggctgga agactaggaa
     8881 atgatacgga caggctaact attaacgcag attattcggg ttgctgctaa aaccaactct
     8941 aataatagtt agtgcaaggg ctggttgagc ttaaattgtc tgataaagag ttctctcttt
     9001 atactgcaaa agaagcgcag ttattcacga ttaggataac tgtttgagag agcctaaggg
     9061 cttgaccctt gatggtttaa gcaccgctat gcgtgcggga tccttatttg ctgaaaatga
     9121 ggaattaaaa aaagaaatta aggacttaaa aatgaagaaa gcagacaagt aagcctccta
     9181 aattcacttt agataaaaat ttaggaggca tatcaaatga actttaataa aattgattta
     9241 gacaattgga agagaaaaga gatatttaat cattatttga accaacaaac gacttttagt
     9301 ataaccacag aaattgatat tagtgtttta taccgaaaca taaaacaaga aggatataaa
     9361 ttttaccctg catttatttt cttagtgaca agggtgataa actcaaatac agcttttaga
     9421 actggttaca atagcgacgg agagttaggt tattgggata agttagagcc actttataca
     9481 atttttgatg gtgtatctaa aacattctct ggtatttgga ctcctgtaaa gaatgacttc
     9541 aaagagtttt atgatttata cctttctgat gtagagaaat ataatggttc ggggaaattg
     9601 tttcccaaaa cacctatacc tgaaaatgct ttttctcttt ctattattcc atggacttca
     9661 tttactgggt ttaacttaaa tatcaataat aatagtaatt accttctacc cattattaca
     9721 gcaggaaaat tcattaataa aggtaattca atatatttac cgctatcttt acaggtacat
     9781 cattctgttt gtgatggtta tcatgcagga ttgtttatga actctattca ggaattgtca
     9841 gataggccta atgactggct tttataagtc gacgcatgcg gcaaagcact caaaagttta
     9901 ccttatgggt gcttttttcg tgcttttttg aaaagacaaa aaaaagaacc ttgccaagca
     9961 agattctttt gatagggtgg tctccgatcg tgtactgcag aaaactagtc gcaaaaaacc
    10021 ccgcttcggc ggggtttttt cgctctagac ggtgatcaac acgctagcca ggcatcaaat
    10081 aaaacgaaag gcttagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
    10141 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatagaat
    10201 tcggtcgaca gatctccatt ctacagttta ttcttgacat tgcactgtc
//
