LOCUS       sequence-455429-        4047 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Integrates KanR into glpK, 570 bp homology, three homologous HIP1 
            sites in left homology arm.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4047)
  AUTHORS   Kamoku C, Nielsen DR
  TITLE     Highly iterated palindrome 1 sequence improves Synechococcus sp. PCC
            7002 transformation efficiencies in a homology- and 
            methylation-dependent manner.
  JOURNAL   Biotechnol Prog. 2025 Jan 23:e3518. doi: 10.1002/btpr.3518.
  PUBMED    39846505
REFERENCE   2  (bases 1 to 4047)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Prog. 2025 Jan 23:e3518. doi: 10.1002/btpr.3518."
COMMENT     Sequence Label: pCMK105
FEATURES             Location/Qualifiers
     source          1..4047
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      139..727
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     628..647
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1564..2358
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1618..1637)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2228..2247
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      2389..2483
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 ttcatccgct tattatcact tattcaggcg tagcaaccag gcgtttaagg gcaccaataa
       61 ctgccttaaa aaaattacgc cccgtgagtt ttcgttccac tgagcgtcag accccgtaga
      121 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
      181 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
      241 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
      301 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
      361 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
      421 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
      481 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
      541 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
      601 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
      661 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
      721 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
      781 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
      841 gtgagcttga ttggctatga tctaccaaag ctggcgattt ttctgcaaac cgaattagaa
      901 ccacaatttg attttttaaa gccgattacc attgccaacc tgaaggaaat agcgatcgcc
      961 tttatggagg atggccacga ggcttgtcgg gtctcccatt attgtggatc ggtaccggat
     1021 gagcgggcga gctttaattt acgcttgcgg caatatcgcc aggcccagcc ttggctgcga
     1081 aatcatctcg atcctgcaag gggcgatcgc ctgcaccatt ggtcggatca ccaacgcacc
     1141 attttctacg gcaggcgcac caatcccgac acccagcagc ggcttgtgtt agtggcgcac
     1201 atggccgggg ctccgaagac cgttgagatt ggcaaatggc tcgccctgga tttggatcgt
     1261 tggcagttgg cgatcgccac accgactttg aagatcaaca ccatctatga cttagcccaa
     1321 attcacttgc acaatggcga aggttttctg ttatctgaaa ttcctcccta aatgatgtct
     1381 tttgagccta aaaacacact tttttgacct aatttaaccc agacgtcgga attgccagct
     1441 ggggcgccct ctggtaaggt tgggaagccc tgcaaagtaa actggatggc tttcttgccg
     1501 ccaaggatct gatggcgcag gggatcatga tctgatcaag agacaggatg aggatcgttt
     1561 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
     1621 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
     1681 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
     1741 ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
     1801 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
     1861 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
     1921 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
     1981 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
     2041 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc
     2101 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa
     2161 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
     2221 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
     2281 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
     2341 cttgacgagt tcttctgagc gggactctgg ggttcgagag ctcgcttgga ctcctgttga
     2401 tagatccagt aatgacctca gaactccatc tggatttgtt cagaacgctc ggttgccgcc
     2461 gggcgttttt tattggtgag aatccaagca ctagtcattc cccctcttgc ctacagcatc
     2521 tcccccaggg gagaattctt cctgtttcaa ctccctctaa cgtaaaccca ttgaatttaa
     2581 aaaagacttt atgactgctt tactgctcca tgaccaacat tattcccttg atcatgaagc
     2641 ctttctctca accctcagca acacagaaaa tttactcatt attcaagatc tagatggcgt
     2701 ttgcatgggg ttagtcaaag accccttaac ccgcaaaatt gatcctgact atatccgcgc
     2761 cacacgcaag tttagagacc acttctttgt cctcaccaac ggtgaacatg aaggcagaag
     2821 gggagtaaat cgcatcgttg aacgggcatt tcgcaatgtt gaagccaaag aggaaacaag
     2881 ctatttacct ggtttagcag cagggggtgt gcaatggcag acagataatg gccaaatttc
     2941 ccatcccggt gttagccaag cagaactcga tttccttgcc acagtgccag atttaattgg
     3001 tcaaagttta ggacaatttt ttactaaata tgttgatatt tttcccgctg agcttcaacc
     3061 tgagctgatc catgcttctg ttttagataa tctcgtttct cgcacctcgc taacggattc
     3121 accactccaa gaattggagc caatcaattc ttgcggagaa ctgtgaatgc gcaaaccaac
     3181 ccttggcaga acatatccat cgcgtccgcc atctccagca gccgcacgcg gcgcatctcg
     3241 ggcagcgttg ggtcctggcc acgggtgcgc atgatcgtgc tcctgtcgtt gaggacccgg
     3301 ctaggctggc ggggttgcct tactggttag cagaatgaat caccgatacg cgagcgaacg
     3361 tgaagcgact gctgctgcaa aacgtctgcg acctgagcaa caacatgaat ggtcttcggt
     3421 ttccgtgttt cgtaaagtct ggaaacgcgg aagtccccta cgtgctgctg aagttgcccg
     3481 caacagagag tggaaccaac cggtgatacc acgatactat gactgagagt caacgccatg
     3541 agcggcctca tttcttattc tgagttacaa cagtccgcac cgctgccggt agctccttcc
     3601 ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt atcatgcaac
     3661 tcgtaggaca ggtgccggca gcgcccaaca gtcccccggc cacggggcct gccaccatac
     3721 ccacgccgaa acaagcgccc tgcaccatta tgttccggat ctgcatcgca ggatgctgct
     3781 ggctaccctg tggaacacct acatctgtat taacgaagcg ctaaccgttt ttatcaggct
     3841 ctgggaggca gaataaatga tcatatcgtc aattattacc tccacgggga gagcctgagc
     3901 aaactggcct caggcatttg agaagcacac ggtcacactg cttccggtag tcaataaacc
     3961 ggtaaaccag caatagacat aagcggctat ttaacgaccc tgccctgaac cgacgaccgg
     4021 gtcgaatttg ctttcgaatt tctgcca
//
