LOCUS       sequence-455439-        4195 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pCB2.4 - pBR322 shuttle vector that replicates in E. coli and 
            Synechococcus sp. PCC 7002, contains HIP1 site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4195)
  AUTHORS   Kamoku C, Cooper C, Straub A, Miller  N, Nielsen DR
  TITLE     Delivery of Novel Replicating Vectors to Synechococcus sp. PCC 7002 
            Via Natural Transformation of Plasmid Multimers
  JOURNAL   Synthetic Biology and Engineering 2024, 2 (3), 10014
REFERENCE   2  (bases 1 to 4195)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synthetic 
            Biology and Engineering 2024, 2 (3), 10014"
COMMENT     Sequence Label: pCMK188
FEATURES             Location/Qualifiers
     source          1..4195
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      42..630
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     531..550
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             3271..4065
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3325..3344)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3935..3954
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      4088..4182
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
       61 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
      121 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
      181 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
      241 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
      301 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
      361 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
      421 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
      481 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
      541 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
      601 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
      661 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
      721 gataaccgta ttaccgcctt tgagtgagct ggagtttctg gtattttcta gcagtatgcc
      781 tacatttccc cgtccccctt gtgttcatag tctgattagt tcaccttgtc ggttgtcaag
      841 ggcacgaagt ggtgaacgaa gtgaacgcac ccttgactaa cgacaaggtg aacggattct
      901 cttttgacaa gggggacggg cttatgggtt ctattactat acttgaggtg ttatattttc
      961 caggtgctct aaaaaattag aatttaattg gcatcttagt ctatttggtg ctaaatttaa
     1021 taggtatcta atgttcattg cgaattacct atgcttcaaa aaagactatc tgatgatgtt
     1081 cctcagccta atgattttat ccgggcttta attaatggtg gtgtctcgac ttctggtgat
     1141 atggactctt tatctaaaaa gcgttctttt accattgaat ttactaatta tgcttttatt
     1201 caagcaatag taaatcttca gtcccctgaa tcttctatta atgcaaataa ggttcttaat
     1261 tactgtttgc gattttatat tgaatatctt caggattctt tacctgataa ggatttgaat
     1321 aagttagttg acgcttatga taaagcttat caagctttaa ttgatgaaga attgcctggt
     1381 ctaaatgagg gttttgactt tttaaagggg cggcgttcta atgtctaaat atcaagttct
     1441 ttacgttact aaatctgagt ttcaagatag aattactggg cggttagttc aaagtttaaa
     1501 ggtccaatac gctagtcctg atgcggttaa tactgataat gcaaaggggc ttcctgcttt
     1561 gactgttccg gctgaatttt ctctctggtc acagtttcgg caagttccgg gggcttatga
     1621 cttggaattt gcgtcaatac ctgacgggcg gggtcgtcct caacagacaa ttactggggt
     1681 taagctccaa gcttgatgct atttcctttg ggcattaaca acaaaaaaat aggctccctt
     1741 tgcgaattac cagttctgag ggggcctttc ttcaaacaaa tattaaatac tcattatgac
     1801 tttatcacta gactcggttg ccctggtagg caaaacccca tttggtaaca cggggttgaa
     1861 atgccaagag cttaaacctg atggaagctg gttacctagc agggggcgaa atatgcttct
     1921 cgatgggggt caacacgagg tggcgatcca ctggattcaa gggactttcc tgattaattc
     1981 cggggtgaca ggggaagctg agttggtgcc actccaggtt aagcagttgg cgcagtatgt
     2041 agccaatgcc actagggaaa ctttggagta ttgtccgggg gttggattgc caaggggtat
     2101 ctattgggaa cattctgcga ggtttggttg tggctctatt ctggcttggc actatgaaag
     2161 caatgggttt tggcgggtct ttctctcgat tccgggtcag gtatgtgagc ggttgggcct
     2221 gatggaaagt gctcgattag tcacggggtt aaaatgccat tttggttttg cggctaccag
     2281 ggttgatgcc aaggttcgtg tttcgtcttg gattctcact cctcagcagg catatcagga
     2341 aggaatacag ggaaacttat cgagggtgtt tgatgtcagt gagcaaacta gtacagtcaa
     2401 gggttatacc agtatgacag cgtatttcgg ttctaagggt agtaatgcta gaactagaat
     2461 atatgacccg tatcataatc acggggttat cggtggcacg gatattgagg cagaattgaa
     2521 agatgagaaa gcaaaagagt ttgttaacgt cttggctgac ttataccaaa ctgcggtaga
     2581 cgaaaaagat ttttcggtat gtgaacaata tgtagcgggg tgtgctgttg ggcaagttga
     2641 ctttttagat agaagtacag gtaagcgggc tagtcggtgt gagcgttgct cctggtggca
     2701 gttggttctt gatactgtgg ggactccgtg ccgggttgtt ggggcaaaag aaactaccag
     2761 tctgattaac acagcggcgt gggcagtgtc gcaagtagtt cccgctttgg cggcgttaaa
     2821 gcggttgacc ggtggcgaaa atttaatgtc ttggctgtca gtacaaatta ataaagtcga
     2881 taatttatca ggcggtatgc gacaaaaagt taatcaggca aaaattgacg gttttacttt
     2941 taatcaagtg gtgcggttag caacttgtgc ctagtggtgg tgtgccgccg tcacgaagta
     3001 ggcgtgctat tttttcaggt ctaaactaat ttaataagta ttttgttatt tattagtttt
     3061 agtaataaaa tatcaaaact attagtcaaa tttggggcag ttttcccttt gatgaacgaa
     3121 aactcacgga cgtcggaatt gccagctggg gcgccctctg gtaaggttgg gaagccctgc
     3181 aaagtaaact ggatggcttt cttgccgcca aggatctgat ggcgcagggg atcatgatct
     3241 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt
     3301 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
     3361 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
     3421 accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg
     3481 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
     3541 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgca
     3601 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
     3661 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
     3721 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
     3781 ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
     3841 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
     3901 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
     3961 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
     4021 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggcga
     4081 tcgcttggac tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc
     4141 agaacgctcg gttgccgccg ggcgtttttt attggtgaga atccaagcac tagtc
//
