LOCUS       sequence-455440-        2731 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Broad host range WVO1 vector that replicates in Synechococcus sp. 
            PCC 7002, KanR, contains HIP1 site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Kamoku C, Cooper C, Straub A, Miller  N, Nielsen DR
  TITLE     Delivery of Novel Replicating Vectors to Synechococcus sp. PCC 7002 
            Via Natural Transformation of Plasmid Multimers
  JOURNAL   Synthetic Biology and Engineering 2024, 2 (3), 10014
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synthetic 
            Biology and Engineering 2024, 2 (3), 10014"
COMMENT     Sequence Label: pCMK74
FEATURES             Location/Qualifiers
     source          1..2731
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      64..150
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             1481..1492
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      complement(1694..1788)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1806..2600)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEEYRCVKDTEGMSPAKVYKLVGENEILYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEGYED
                     EQSPEKTIELYAECIRLFHSIDISDCPYTNSLDGRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 ttttcaaagt tggcgtataa catagtatcg acggagccga ttttgaaacc acaccagagg
       61 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
      121 tcggtgaacg ctctcctgag taggacaaat ccgccgccct agacctagtg tcattttatt
      181 tcccccgttt cagcatcaag aacctttgca taacttgctc tatatccaca ctgataattg
      241 ccctcaaacc ataatctaaa ggcgctagag tttgttgaaa caatatcttt tacatcattc
      301 gtatttaaaa ttccaaactc cgctccccta aggcgaataa aagccattaa atcttttgta
      361 tttaccaaat tatagtcatc cactatatct aagagtaaat tcttcaattc tcttttttgg
      421 ctttcatcaa gtgttatata gcggtcaata tcaaaatcat taatgttcaa aatatctttt
      481 ttgtcgtata tatgtttatt cttagcaata gcgtcctttg attcatgagt caaatattca
      541 tatgaacctt tgatataatc aagtatctca acatgagcaa ctgaactatt ccccaatttt
      601 cgcttaatct tgttcctaac gctttctatt gttacaggat ttcgtgcaat atatataacg
      661 tgatagtgtg gttttttata gtgctttcca tttcgtataa catcactact attccatgta
      721 tctttatctt ttttttcgtc catatcgtgt aaaggactga cagccataga tacgcccaaa
      781 ctctctaatt tttccttcca atcattagga attgagtcag gatataataa aaatccaaaa
      841 tttctagctt tagtattttt aatagccatg atataattac cttatcaaaa acaagtagcg
      901 aaaactcgta tccttctaaa aacgcgagct ttcgcttatt ttttttgttc tgattccttt
      961 cttgcatatt cttctatagc taacgccgca accgcagatt ttgaaaaacc tttttgtttc
     1021 gccatatctg ttaatttttt atcttgctct tttgtcagag aaatcataac tctttttttc
     1081 gattctgaaa tcaccattta aaaaactcca atcaaataat tttataaagt tagtgtatca
     1141 ctttgtaatc ataaaaacaa caataaagct acttaaatat agatttataa aaaacgttgg
     1201 cgaaaacgtt ggcgattcgt tggcgattga aaaacccctt aaacccttga gccagttggg
     1261 atagagcgtt tttggcacaa aaattggcac tcggcactta atggggggtc gtagtacgga
     1321 agcaaaattc gcttcctttc cccccatttt tttccaaatt ccagattttt ttcaaaaatt
     1381 ttccagcgct accgctcggc aaaattgcaa gcaattttta aaatcaaacc catgagggaa
     1441 tttcattccc tcatactccc ttgagcctcc tccaaccgaa atagaagggc gctgcgctta
     1501 ttatttcatt cagtcatcgg ctttcataat ctaacagaca acatcttcgc tgcaaagcca
     1561 cgctacgctc aagggctttt acgctacgat aacgcctgtt ttaacgatta tgccgataac
     1621 taaacgaaat aaacgctaaa acgtctcaga aacgattttg agacgtttta ataaaaaatc
     1681 gcctagtgct tggattctca ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca
     1741 aatccagatg gagttctgag gtcattactg gatctatcaa cgggagtcca agcgatcgct
     1801 cggtactaaa acaattcatc cagtaaaata taatatttta ttttctccca atcaggcttg
     1861 atccccagta agtcaaaaaa tagctcgaca tactgttctt ccccgatatc ctccctgatc
     1921 gaccggacgc agaaggcaat gtcataccac ttgtccgccc tgccgcttct cccaagatca
     1981 ataaagccac ttactttgcc atctttcaca aagatgttgc tgtctcccag gtcgccgtgg
     2041 gaaaagacaa gttcctcttc gggcttttcc gtctttaaaa aatcatacag ctcgcgcgga
     2101 tctttaaatg gagtgtcttc ttcccagttt tcgcaatcca catcggccag atcgttattc
     2161 agtaagtaat ccaattcggc taagcggccg tctaagctat tcgtataggg acaatccgat
     2221 atgtcgatgg agtgaaagag cctgatgcac tccgcataca gctcgatagt cttttcaggg
     2281 ctttgttcat cttcataccc ttccgagcaa aggacgccat cggcctcact catgagcaga
     2341 ttgctccagc catcatgccg ttcaaagtgc aggacctttg gaacaggcag ctttccttcc
     2401 agccatagca tcatgtcctt ttcccgttcc acatcatagg tggtcccttt ataccggctg
     2461 tccgtcattt ttaaatatag gatttcattt tctcccacca gcttatatac cttagcagga
     2521 gacattcctt ccgtatcttt tacgcagcgg tattcttcga tcagtttttt caattccggt
     2581 gatattctca ttttagccat ttattatttc cttcctcttt tctacagtat ttaaagatac
     2641 cccaagaagc taattataac aagacgaact ccaattcact gttccttgca ttctaaaacc
     2701 ttaaatacag aaaacagcct tttcaaagtt g
//
