LOCUS       sequence-455446-         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  KHC064 (pX335 BRD2 K1-1a), gRNA and cas9D10A mammalian expression 
            vector for CRISPR mediated cutting of BRD2 at the N-terminus, use 
            with KHC065.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Bond AG, Craigon C, Chan KH, Testa A, Karapetsas A, Fasimoye R, 
            Macartney T, Blow JJ, Alessi DR, Ciulli A
  TITLE     Development of BromoTag: A ""Bump-and-Hole""-PROTAC System to Induce
            Potent, Rapid, and Selective Degradation of Tagged Target Proteins.
  JOURNAL   J Med Chem. 2021 Oct 28;64(20):15477-15502. doi: 
            10.1021/acs.jmedchem.1c01532. Epub 2021 Oct 15.
  PUBMED    34652918
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.1c01532"; journalName: "J Med Chem"; date: 
            "2021-10-28- 28"; volume: "64"; issue: "20"; pages: "15477-15502"
COMMENT     Sequence Label: KHC064 pX335 BRD2 K1-1a
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     130..149
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        229..304
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        401..686
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 gtttgttagg ctgttgagag tattgggatt aatttgactg taaacacaaa gatattagta
       61 caaaatacgt gacgtagaaa gtaataattt cttgggtagt ttgcagtttt aaaattatgt
      121 tttaaaatgg actatcatat gcttaccgta acttgaaagt atttcgattt cttggcttta
      181 tatatcttgt ggaaaggacg aaacaccgag ggcagcgccg gttccttggt tttagagcta
      241 gaaatagcaa gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg
      301 gtgctttttt gttttagagc tagaaatagc aagttaaaat aaggctagtc cgtttttagc
      361 gcgtgcgcca attctgcaga caaatggctc tagaggtacc cgttacataa cttacggtaa
      421 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata gtaacgccaa
      481 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      541 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
      601 ccgcctggca ttgtgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
      661 acgtattagt catcgctatt accatggtcc aaggtgaacc cccacgttct gcttcactct
      721 cccccattct cccccccctt ccccaccccc aaattttgga attaatttat tttttaaatt
      781 attttgtggc aaccaatggg ggcggggggg gggggggggg g
//
