LOCUS       sequence-455447-        1260 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR mediated KI of BromoTag at N-terminus of 
            BRD2-GFP-P2A-BromoTag, works well with KHC064 and KHC065.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Bond AG, Craigon C, Chan KH, Testa A, Karapetsas A, Fasimoye R, 
            Macartney T, Blow JJ, Alessi DR, Ciulli A
  TITLE     Development of BromoTag: A ""Bump-and-Hole""-PROTAC System to Induce
            Potent, Rapid, and Selective Degradation of Tagged Target Proteins.
  JOURNAL   J Med Chem. 2021 Oct 28;64(20):15477-15502. doi: 
            10.1021/acs.jmedchem.1c01532. Epub 2021 Oct 15.
  PUBMED    34652918
REFERENCE   2  (bases 1 to 1260)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.1c01532"; journalName: "J Med Chem"; date: 
            "2021-10-28- 28"; volume: "64"; issue: "20"; pages: "15477-15502"
COMMENT     Sequence Label: KHC068 BRD2-KI-GFP-2A-L387A
FEATURES             Location/Qualifiers
     source          1..1260
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(974..1030)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(1082..1103)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ggaactgcga aggtttccga ctggaaagcg ggcagtgaaa ggaaggccca tgaggcccag
       61 taggaacctg cacacacggg agagtgatcc ctttaccctt ctctgggaat ccccgttgcc
      121 agtcccttaa gcgctccgtc accatggcaa ccctacaggg cgctagaggt gtccgtgaat
      181 cccccctaaa aggctaacag accgccgcta gataccagac ccaccaaacg ccaaacctgc
      241 tgttcaacct cactccactc caccgtcaaa ctagctctgc ctgactcggt taagactggg
      301 ccacggcggc attaatagcg tataaattga caacctgtat tcccctccac gagcagtagc
      361 cgagccgcca ccaccgttcc aaaaccgtcg ctcaattccc tccgcgcgac cgagtcgaca
      421 gctgcgccgc aacagcagcg cgccggggcc gctcagtact cccaacacgg cgccccacac
      481 ccctgtgccg ccgccctgca acatcccctt cccagacgcg gctcgcggaa acgtacttat
      541 tgtggggagt cacgttttgc aggccacctc cgcctccgcc ctcgtccggc atcttggcaa
      601 agcgcatttc gaacacatcc tggagcttgc gggccatggc caccacctca tggtcaggag
      661 ggttgtactt atagcagttg gagaacatca atcggacgtc agcaccaaac tcctgagcat
      721 cacggtactc acgggcctcc agtttagact tgattgtgct catgtccatg gggtgcttga
      781 tgatgtcaca gtagtcgtgg gcgcccagtg cctccacgtc cacaggcttg tagaagggcc
      841 aggcgtaggc ggcgtgcttc ttggcaaaca tctccttgag gatgccgctg cagcacttga
      901 gctgctccga gaccttgctg ctcttctctg gtgctgggtg ctgctgagag tcgggcacgt
      961 ccttcacgga cccaggcccg ggattctcct ccacgtcacc tgcttgtttg agtagtgaga
     1021 agtttgttgc tccggatccc ttgtacagct cgtccatgcc gagagtgatc ccggcggcgg
     1081 tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt ggggtctttg ctcagggcgg
     1141 actggggtgc tcaggtagtg gttgtcgggc accaccacgg ggccgtcccc caatgggggt
     1201 gttctgctgg taatggtccg gcaagctgca ccctgccgtc ctccaatgtt tggggcggaa
//
