LOCUS       sequence-455456-        4369 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  L0 part - CDS (without STOP codon).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4369)
  AUTHORS   Golubova D, Salmon M, Su H, Tansley C, Kaithakottil GG, Linsmith G, 
            Schudoma C, Swarbreck D, O'Connell MA, Patron NJ
  TITLE     Biosynthesis and bioactivity of anti-inflammatory triterpenoids in 
            Calendula officinalis.
  JOURNAL   Nat Commun. 2025 Jul 28;16(1):6941. doi: 10.1038/s41467-025-62269-w.
  PUBMED    40721615
REFERENCE   2  (bases 1 to 4369)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-07-28"; volume: "16(1):6941. doi"; pages: " 
            10.1038/s41467-025-62269-w"
COMMENT     Sequence Label: pEPDG1CB0027
FEATURES             Location/Qualifiers
     source          1..4369
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2413..2429)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2413..2429)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2426..2448)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(2601..3189)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2681..2700)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3322..3416)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(3437..4096)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     4011..4030
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(4097..4199)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     4286..4308
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4300..4317
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4301..4317
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 taggtctcta atgtggaaat tgaaaattgg tgagaaaaat ggaaagctgg aaattggtga
       61 cggaaatggc gatgaatatt tgtatagtac caacaatttt gtgggcagac aaatatggga
      121 gttcgatcca agtgcaggca cgcaggaaga acgtgaccaa atcgaaagga ttcgagagca
      181 gttcttgaac aataagaaga aactcgacat ccattgttgt ggtgacttgc tcatgcgaaa
      241 ccagcttatc aaggaaagcc ggattgatct tactagtgaa cctttaatga aactcaaaga
      301 tgaagaggac gttaactatg aagccgtgac gacggcggtt aaaaaagcgg tacgcttgaa
      361 ccgtgcaatt caggcttggg acggtcattg gccggctgaa aatgcgggtc cccttttttt
      421 cacccctcct ctgataattg ccttgtacat aagtggtaca atggatacaa ttttatcaaa
      481 agaacaccag aaggagatga ttcgctacat gtacatccat caaaatgaag atggaggatg
      541 gggattctat atatcgggca aaagcacgat gattggaagt gctttgaact atgtggctct
      601 aagacttctt ggagaagctt caaccgatga tgacattaat ggtgacggtg cacttgctag
      661 agcccaaaaa tggatctttg atcacggtgg tgctacatcc atcccttcat ggggcaaggt
      721 ttatctggct gtgcttgggg tatatgaatg ggaaggctgc aatcctctgc ctccagaatt
      781 ttggctattt ccttcctttt tgccttatca tccagcaaaa atgtggtgtt actgtagaac
      841 gacatacatg ccgatgtcac acttgtatgg taaaagctac catggcccta ttactgatct
      901 tgttctgtct ttgagaaaag agattcacac tcttccttac caccacatta actggaacaa
      961 acaacgacat aactgttgca aggtggatct atactaccct cattcttcca tacaagatct
     1021 gttatgggat agtctgcatt atttttgtga accgcttttc agaaaatggc cctttaacaa
     1081 gctaagagag aagggtctta aaagagtcat tgacctaatg cgttatggat ccgaggaagg
     1141 acgttacata ggcatgggtt gtgttgacaa ggctttacaa atgatgtgtt tttatgcgga
     1201 ggatccaaat gggattgact tcaaacgcca cctggctaga gtccccgatt acttgtgggt
     1261 tgcagaagat ggtatgaaga tgcaaagttt cggaagtcag ttatgggatt gcactcttgt
     1321 aactcaagca attatggcca gtaatatggt cgatgagtat ggagattcac tcaaaaaagc
     1381 ccacttttac ttaaaacagt cacagattaa agagaaccct aaaggcgatt tcacaaaaat
     1441 gtgtcgttta ttcacgaaag gggcatggac tttctctgat caagatcatg gatgggtcgt
     1501 atcagattgc actgcagaag ctttgaaatg tttattggca ttgtcacaaa tgccacaaga
     1561 tattgctggc gaaaaggctg aagttgatag attatatgat gctgtgaatg tcctccttta
     1621 tctgcaaagt cctgaaagtg ggggtttcgc aatttgggag ccaccagttc caaaaccata
     1681 cttagagatg ctgaacccat cggaactttt tgcagacata gtggttgaga aagagcatgt
     1741 tgaatgtact ggatcaataa tccaagcatt gaacttattc aaaaatctgc acccacggca
     1801 ccgtgagaaa gaaatagaag ttgctatcga gaaaggcata cgttttttgg aaaccaaaca
     1861 acaaaaagat ggttcatggt acggttattg gggtatttgt tttctctatg gcacattttt
     1921 tgttctgcaa ggtttggtat catgcgggaa aacatatgaa aatagtgaaa ccattcgaaa
     1981 agctgtcaaa tttttgctct caacacaaaa tgcagaaggt ggttggggag agagttttga
     2041 gtcatgtcca caagagaaat tcataccttt ggagggaaat agaacaaatt tggtgcagac
     2101 ttcatgggca atgcttggac ttctctatgg tggacaggtt gaaagagatc caacaccgtt
     2161 acaccaggca gcaaaactgc tcattaatgg acaattggat aatggagatt ttccacagaa
     2221 ggaaataact ggagtttaca tgaaaaactg catgttgcat tatccagaat ataggaacac
     2281 ttttccgtta tgggcgttgg gagaatatcg caaccgtgtt tggttgccaa aacaacaagt
     2341 cggttcgaga gacctaaggc taggtggagg ctcagtgatg ataagtctgc gatggtggat
     2401 gcatgtgtca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca gagggcacaa
     2461 tcctattccg cgctatccga caatctccaa gacattaggt ggagttcagt tcggcgtatg
     2521 gcatatgtcg ctggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     2581 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     2641 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     2701 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     2761 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     2821 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     2881 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     2941 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     3001 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     3061 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     3121 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     3181 ggatctcaag aagatccttt gatcttttct acggggtctg acgctctatt caacaaagcc
     3241 gccgtcccgt caagtcagcg taaatgggta gggggcttca aatcgtcccc ccatacgata
     3301 taagttgtta ctagtgcttg gattctcacc aataaaaaac gcccggcggc aaccgagcgt
     3361 tctgaacaaa tccagatgga gttctgaggt cattactgga tctatcaaca ggagtccaag
     3421 cgagctcgat atcaaattac gccccgccct gccactcatc gcagtactgt tgtaattcat
     3481 taagcattct gccgacatgg aagccatcac aaacggcatg atgaacctga atcgccagcg
     3541 gcatcagcac cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga
     3601 agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg
     3661 agacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac
     3721 acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc
     3781 agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat
     3841 cccatatcac cagctcaccg tctttcattg ccatacgaaa ttccggatga gcattcatca
     3901 ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct
     3961 ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact
     4021 gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag
     4081 tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgataac tcaaaaaata
     4141 cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg tgccgatcaa
     4201 cgtctcattg atacctgaaa caaaacccat cgtacggcca aggaagtctc caataactgt
     4261 gatccaccac aagcgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtcat
     4321 gcataatccg cacgcatctg gaataaggaa gtgccattcc gcctgacct
//
