LOCUS       sequence-455457-        3475 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  L0 part - CDS (without STOP codon).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3475)
  AUTHORS   Golubova D, Salmon M, Su H, Tansley C, Kaithakottil GG, Linsmith G, 
            Schudoma C, Swarbreck D, O'Connell MA, Patron NJ
  TITLE     Biosynthesis and bioactivity of anti-inflammatory triterpenoids in 
            Calendula officinalis.
  JOURNAL   Nat Commun. 2025 Jul 28;16(1):6941. doi: 10.1038/s41467-025-62269-w.
  PUBMED    40721615
REFERENCE   2  (bases 1 to 3475)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-07-28"; volume: "16(1):6941. doi"; pages: " 
            10.1038/s41467-025-62269-w"
COMMENT     Sequence Label: pEPDG1CB0028
FEATURES             Location/Qualifiers
     source          1..3475
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1519..1535)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1519..1535)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1532..1554)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(1707..2295)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1787..1806)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2428..2522)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2543..3202)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3117..3136
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(3203..3305)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     3392..3414
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3406..3423
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3407..3423
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 taggtctcta atggagatat tctatgcttc tcttatctct ctttttgttc ttgttgtttc
       61 tttttctctt cattttttct tctacaattc aaaacaaccc gaagatcaga aactacttcc
      121 accgggtaga acgggctggc ccataatcgg agaaagtctc gagtttctcg gaagcggttg
      181 gtacgggcac cccgaaaagt tcatacttga ccgaatgtca aagttttcgc ctatagtatt
      241 taggtcatct ctcttgctag aggatgctgc agtgatgtgt ggtgctcaag ggaacaaatt
      301 cttgttctca aatgagaaca aactagtcca agtatggtgg cccgcttcgg ttgacaaaat
      361 ctttccgtcg gctaacaaaa cctacaaaac cgaaaccatt aaaatgagaa aaatgctccc
      421 gaatttcctc aaacccgaag ccttgcaacg atatattcca atcatggata ttaccacaca
      481 aaaacacttt gccgcgggat gggaagggaa aaaagaagtt gtgacttatg aactcacaag
      541 acactttgct ttttggcttg cgtgcaagat ctttctagat atcgatgatc ctgaacgggt
      601 cagatattta tctggcccgt ttgagagcat cgttacgggg atcctgtcga tccccattga
      661 tcttccgggg acaccgttta gaagagggat caatgggtcc aacttcataa taaaggaact
      721 tgtggatatt gtgaagcaaa ggaaaattga tttggctcaa gggaaagcaa caccaaccca
      781 agatatattg tcacatatga ttctatttag cgacgaagat ggcgagttca tgaccgaaca
      841 tgacatttcg ggcaagattc ttgcgttgct tattggtggg catgacactg cgagctctgc
      901 tacggctttt attgttaagt atcttgccga attgccagaa atctatgatg gagtctacaa
      961 cgaacaaatg gaaattgcaa aatataaagc agctggggaa ttattgaatt gggaggatct
     1021 atcaaagatg aaatactcat ggaaagttgc atgtgaagtt cttagattag tcccaccggc
     1081 ccaaggtgct tttagagaag cccttacaga tttcatgtac aatggttact ctatccccaa
     1141 gggctggaag ttatattgga gtgcaagctc aacccataaa aaccccgagg ttttccccga
     1201 acccgaaaaa tttgacccgt caagatttga tggcagcggg ccagcacctt acacgttcgt
     1261 gccatttgga ggaggcccgc acatgtgtcc cggaaaagag tatgcccgat tagaaatact
     1321 cgtgtttatg catcatcttg taacaaagtt caaatgggag aaaattattc ccgatgagcc
     1381 cgttgttttc aacccgatgc ctagccctgc caagggactt cctattcgta tttaccctca
     1441 taaagctggt tcgagagacc taaggctagg tggaggctca gtgatgataa gtctgcgatg
     1501 gtggatgcat gtgtcatggt catagctgtt tcctgtgtga aattgttatc cgctcagagg
     1561 gcacaatcct attccgcgct atccgacaat ctccaagaca ttaggtggag ttcagttcgg
     1621 cgtatggcat atgtcgctgg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     1681 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     1741 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     1801 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     1861 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     1921 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     1981 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     2041 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     2101 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     2161 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     2221 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     2281 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tctattcaac
     2341 aaagccgccg tcccgtcaag tcagcgtaaa tgggtagggg gcttcaaatc gtccccccat
     2401 acgatataag ttgttactag tgcttggatt ctcaccaata aaaaacgccc ggcggcaacc
     2461 gagcgttctg aacaaatcca gatggagttc tgaggtcatt actggatcta tcaacaggag
     2521 tccaagcgag ctcgatatca aattacgccc cgccctgcca ctcatcgcag tactgttgta
     2581 attcattaag cattctgccg acatggaagc catcacaaac ggcatgatga acctgaatcg
     2641 ccagcggcat cagcaccttg tcgccttgcg tataatattt gcccatggtg aaaacggggg
     2701 cgaagaagtt gtccatattg gccacgttta aatcaaaact ggtgaaactc acccagggat
     2761 tggctgagac gaaaaacata ttctcaataa accctttagg gaaataggcc aggttttcac
     2821 cgtaacacgc cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt
     2881 cactccagag cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa
     2941 cactatccca tatcaccagc tcaccgtctt tcattgccat acgaaattcc ggatgagcat
     3001 tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta
     3061 cggtctttaa aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa
     3121 ctgactgaaa tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat
     3181 atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc gataactcaa
     3241 aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct cttacgtgcc
     3301 gatcaacgtc tcattgatac ctgaaacaaa acccatcgta cggccaagga agtctccaat
     3361 aactgtgatc caccacaagc gccagggttt tcccagtcac gacgttgtaa aacgacggcc
     3421 agtcatgcat aatccgcacg catctggaat aaggaagtgc cattccgcct gacct
//
