LOCUS       sequence-455501-        5806 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The plasmid contains the human WIPI1 promotor sequence (-1226 bp 
            upstream to +329 bp downstream of the transcription start site) 
            cloned into pGL4.23[luc2/minP] (NheI/HindIII)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5806)
  AUTHORS   Sporbeck K, Haas ML, Pastor-Maldonado CJ, Schussele DS, Hunter C, 
            Takacs Z, Diogo de Oliveira AL, Franz-Wachtel M, Charsou C, 
            Pfisterer SG, Gubas A, Haller PK, Knorr RL, Kaulich M, Macek B, 
            Eskelinen EL, Simonsen A, Proikas-Cezanne T
  TITLE     The ABL-MYC axis controls WIPI1-enhanced autophagy in lifespan 
            extension.
  JOURNAL   Commun Biol. 2023 Aug 24;6(1):872. doi: 10.1038/s42003-023-05236-9.
  PUBMED    37620393
REFERENCE   2  (bases 1 to 5806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2023-08-24"; volume: "6(1):872. doi"; pages: " 
            10.1038/s42003-023-05236-9"
COMMENT     Sequence Label: pGL4.23-WIPI1promotor
FEATURES             Location/Qualifiers
     source          1..5806
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1601..1632
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      1659..1668
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1665..3317
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    3361..3482
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3372..3391)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3426..3445
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3730..3747)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3901..4489)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3981..4000)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4689..5480,5481..5549))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    5654..5702
                     /note="synthetic polyadenylation signal"
     misc_feature    join(5716..5806,1)
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     5756..5775
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 tggcctaact ggccggtacc tgagctcgct agcccaaagc tggcagagct cctgcttcat
       61 aaaccgtatt gacagtaatt ccgcatggga acatgtacag tactcagctt ttaaaaagag
      121 attctttgtt aaggtttatc tcgaattacg tgagttttga attgtgctat atcttcaaga
      181 cgttacccct ttcataaact acactgccct acacacactt tcattttttt cagctgcgga
      241 tcggataggt cttgtttaga tactttgtgc catttttgta acttttcaga tcaaaggtgc
      301 ttccccccac cttccagctc cgtcaaatgc tgcagtcatt tctgtcctgg ttagaaaacc
      361 tagaatctcg catgctaggt gctacgttgt ttttgcaaat tactgtttag cagttattta
      421 gtgcacaggt cttgaaagca gacatgcaga cattctacca tttactgtta ctttagacaa
      481 attgtttagc tttggagctt cagttgcctc ttctgtatat tgagcacaat cgtattttcc
      541 acacaagttt tataagaatt caagatgaca tacatagtac ggcgcgcttg gcaagtagtg
      601 agggttctca accgctcggc aatggataag ataacttgct atctctctgg ctgaggcagc
      661 cccatttcag gagcttctct caatgcggct tgaggactcg acaaaagggt ggggctttgg
      721 agtctaacgc tctctgctaa actctgtaag tttaataggc agagttacac atggcaaggg
      781 gtctgggcgg ggtgggaact agcgcgtcct cctgtcaccc ttgcggcggc cccgacgcag
      841 ttgccggact gaaaccagca gatctggccc cagccgcatt ccttcttccc tctccctccc
      901 agctgggcgg ctccggcaat gaaaacaccg ccacgtgggc ctgctgcctt tgcgtcacgc
      961 gcctttgtcc aatggagagc cgcgttacga agccaggggc gggtcgccag ccgggcgggt
     1021 ccgcccacag ctgccacgga ttggccaagg ggggcggtgc ccgccagccg tggcttgggc
     1081 tcgtggccaa tggtggccgg aggcaggggc cccgccctat caggtgctct ggtcctcggg
     1141 accagctgga ggggcgagaa ggggcggggc gaagccgggg acgcggcggg gacggggcgg
     1201 ggtaggggcg gggtggggac ggggcggggt aggggcgggg tggggacggg gcggggtagg
     1261 gcggggcagg accggcgacc cggcccgtgg agccggcgcg ggcgggctgc tgaggtggct
     1321 gtcgccggct ccgagctgcg gcttcccggg ccgagccccc gatggaggcc gaggccgcgg
     1381 acgctccccc gggcggggtt gagtcggcgc tcagctgctt ctctttcaac caggactgca
     1441 cgtaagctgc gactcggccc ctgccaggga ggtgggggac agagtgtcag gacccagggg
     1501 tgtcgtcctg agagcagctt gtggcttccg cctcgaggcg gcctcggcgg gagggccggt
     1561 gtctgcttcg gacgcattgg gatcttgtaa gcttagacac tagagggtat ataatggaag
     1621 ctcgacttcc agcttggcaa tccggtactg ttggtaaagc caccatggaa gatgccaaaa
     1681 acattaagaa gggcccagcg ccattctacc cactcgaaga cgggaccgcc ggcgagcagc
     1741 tgcacaaagc catgaagcgc tacgccctgg tgcccggcac catcgccttt accgacgcac
     1801 atatcgaggt ggacattacc tacgccgagt acttcgagat gagcgttcgg ctggcagaag
     1861 ctatgaagcg ctatgggctg aatacaaacc atcggatcgt ggtgtgcagc gagaatagct
     1921 tgcagttctt catgcccgtg ttgggtgccc tgttcatcgg tgtggctgtg gccccagcta
     1981 acgacatcta caacgagcgc gagctgctga acagcatggg catcagccag cccaccgtcg
     2041 tattcgtgag caagaaaggg ctgcaaaaga tcctcaacgt gcaaaagaag ctaccgatca
     2101 tacaaaagat catcatcatg gatagcaaga ccgactacca gggcttccaa agcatgtaca
     2161 ccttcgtgac ttcccatttg ccacccggct tcaacgagta cgacttcgtg cccgagagct
     2221 tcgaccggga caaaaccatc gccctgatca tgaacagtag tggcagtacc ggattgccca
     2281 agggcgtagc cctaccgcac cgcaccgctt gtgtccgatt cagtcatgcc cgcgacccca
     2341 tcttcggcaa ccagatcatc cccgacaccg ctatcctcag cgtggtgcca tttcaccacg
     2401 gcttcggcat gttcaccacg ctgggctact tgatctgcgg ctttcgggtc gtgctcatgt
     2461 accgcttcga ggaggagcta ttcttgcgca gcttgcaaga ctataagatt caatctgccc
     2521 tgctggtgcc cacactattt agcttcttcg ctaagagcac tctcatcgac aagtacgacc
     2581 taagcaactt gcacgagatc gccagcggcg gggcgccgct cagcaaggag gtaggtgagg
     2641 ccgtggccaa acgcttccac ctaccaggca tccgccaggg ctacggcctg acagaaacaa
     2701 ccagcgccat tctgatcacc cccgaagggg acgacaagcc tggcgcagta ggcaaggtgg
     2761 tgcccttctt cgaggctaag gtggtggact tggacaccgg taagacactg ggtgtgaacc
     2821 agcgcggcga gctgtgcgtc cgtggcccca tgatcatgag cggctacgtt aacaaccccg
     2881 aggctacaaa cgctctcatc gacaaggacg gctggctgca cagcggcgac atcgcctact
     2941 gggacgagga cgagcacttc ttcatcgtgg accggctgaa gagcctgatc aaatacaagg
     3001 gctaccaggt agccccagcc gaactggaga gcatcctgct gcaacacccc aacatcttcg
     3061 acgccggggt cgccggcctg cccgacgacg atgccggcga gctgcccgcc gcagtcgtcg
     3121 tgctggaaca cggtaaaacc atgaccgaga aggagatcgt ggactatgtg gccagccagg
     3181 ttacaaccgc caagaagctg cgcggtggtg ttgtgttcgt ggacgaggtg cctaaaggac
     3241 tgaccggcaa gttggacgcc cgcaagatcc gcgagattct cattaaggcc aagaagggcg
     3301 gcaagatcgc cgtgtaataa ttctagagtc ggggcggccg gccgcttcga gcagacatga
     3361 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
     3421 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
     3481 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
     3541 tttaaagcaa gtaaaacctc tacaaatgtg gtaaaatcga taaggatccg tcgaccgatg
     3601 cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc
     3661 gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc
     3721 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     3781 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     3841 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     3901 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gttagaggtg
     3961 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     4021 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     4081 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     4141 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     4201 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     4261 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     4321 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     4381 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     4441 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     4501 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     4561 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     4621 atcaatctaa agtatatatg agtaaacttg gtctgacagc ggccgcaaat gctaaaccac
     4681 tgcagtggtt accagtgctt gatcagtgag gcaccgatct cagcgatctg cctatttcgt
     4741 tcgtccatag tggcctgact ccccgtcgtg tagatcacta cgattcgtga gggcttacca
     4801 tcaggcccca gcgcagcaat gatgccgcga gagccgcgtt caccggcccc cgatttgtca
     4861 gcaatgaacc agccagcagg gagggccgag cgaagaagtg gtcctgctac tttgtccgcc
     4921 tccatccagt ctatgagctg ctgtcgtgat gctagagtaa gaagttcgcc agtgagtagt
     4981 ttccgaagag ttgtggccat tgctactggc atcgtggtat cacgctcgtc gttcggtatg
     5041 gcttcgttca actctggttc ccagcggtca agccgggtca catgatcacc catattatga
     5101 agaaatgcag tcagctcctt agggcctccg atcgttgtca gaagtaagtt ggccgcggtg
     5161 ttgtcgctca tggtaatggc agcactacac aattctctta ccgtcatgcc atccgtaaga
     5221 tgcttttccg tgaccggcga gtactcaacc aagtcgtttt gtgagtagtg tatacggcga
     5281 ccaagctgct cttgcccggc gtctatacgg gacaacaccg cgccacatag cagtactttg
     5341 aaagtgctca tcatcgggaa tcgttcttcg gggcggaaag actcaaggat cttgccgcta
     5401 ttgagatcca gttcgatata gcccactctt gcacccagtt gatcttcagc atcttttact
     5461 ttcaccagcg tttcggggtg tgcaaaaaca ggcaagcaaa atgccgcaaa gaagggaatg
     5521 agtgcgacac gaaaatgttg gatgctcata ctcgtccttt ttcaatatta ttgaagcatt
     5581 tatcagggtt actagtacgt ctctcaagga taagtaagta atattaaggt acgggaggta
     5641 ttggacaggc cgcaataaaa tatctttatt ttcattacat ctgtgtgttg gttttttgtg
     5701 tgaatcgata gtactaacat acgctctcca tcaaaacaaa acgaaacaaa acaaactagc
     5761 aaaataggct gtccccagtg caagtgcagg tgccagaaca tttctc
//
