LOCUS       sequence-455503-        8568 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BioID experiment.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8568)
  TITLE     Ori Lab colocalization and BioID plasmids 2024
REFERENCE   2  (bases 1 to 8568)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pcDNA-pDEST_BirA_Flag_SLC45A1
FEATURES             Location/Qualifiers
     source          1..8568
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(859..906)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     1038..1057
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    1190..1414
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     1403..1420
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     protein_bind    1462..1486
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3872..3896)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(3909..3932)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(join(3963..4571,4572..4574,4575..4925))
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     primer_bind     complement(5047..5071)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    5081..5099
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5102..5120
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(5122..5325)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(5151..5171)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5326..5705
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     5877..5896
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        5971..6075
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6076..6144,6145..6936)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6294..6313)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7107..7695
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7596..7615
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7849..7866
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7983..8004
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8019..8024,8025..8042,8043..8049)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8057..8073
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8062..8084
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8081..8097
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8081..8097
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    8146..8267
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8157..8176)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8211..8230
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(join(8397..8568,1..851))
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             complement(join(8397..8568,1..851))
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
ORIGIN
        1 tcatcgaaat tgccgtcaac caagctctga tagagttggt caagaccaat gcggagcata
       61 tacgcccgga gccgcggcga tcctgcaagc tccggatgcc tccgctcgaa gtagcgcgtc
      121 tgctgctcca tacaagccaa ccacggcctc cagaagaaga tgttggcgac ctcgtattgg
      181 gaatccccga acatcgcctc gctccagtca atgaccgctg ttatgcggcc attgtccgtc
      241 aggacattgt tggagccgaa atccgcgtgc acgaggtgcc ggacttcggg gcagtcctcg
      301 gcccaaagca tcagctcatc gagagcctgc gcgacggacg cactgacggt gtcgtccatc
      361 acagtttgcc agtgatacac atggggatca gcaatcgcgc atatgaaatc acgccatgta
      421 gtgtattgac cgattccttg cggtccgaat gggccgaacc cgctcgtctg gctaagatcg
      481 gccgcagcga tcgcatccat ggcctccgcg accggctgca gaacagcggg cagttcggtt
      541 tcaggcaggt cttgcaacgt gacaccctgt gcacggcggg agatgcaata ggtcaggctc
      601 tcgctgaatt ccccaatgtc aagcacttcc ggaatcggga gcgcggccga tgcaaagtgc
      661 cgataaacat aacgatcttt gtagaaacca tcggcgcagc tatttacccg caggacatat
      721 ccacgccctc ctacatcgaa gctgaaagca cgagattctt cgccctccga gagctgcatc
      781 aggtcggaga cgctgtcgaa cttttcgatc agaaacttct cgacagacgt cgcggtgagt
      841 tcaggctttt tggccaagga agttcctata ctttctagag aataggaact tcggaatagg
      901 aacttctagg tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
      961 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
     1021 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
     1081 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
     1141 gcgtggggat accccctaga gccccagctg gttctttccg cctcagaagc catagagccc
     1201 accgcatccc cagcatgcct gctattgtct tcccaatcct cccccttgct gtcctgcccc
     1261 accccacccc ccagaataga atgacaccta ctcagacaat gcgatgcaat ttcctcattt
     1321 tattaggaaa ggacagtggg agtggcacct tccagggtca aggaaggcac gggggagggg
     1381 caaacaacag atggctggca actagaaggc acagtcgagg ctgatcagcg ggtttaaacg
     1441 ggccctctag actcgagtca caccactttg tacaagaaag ctgggtcggc gcgcccaccc
     1501 tttcacacat tcagcagcag aggtctgtgc tcctcatcgg cggcgtcgct tggagggatc
     1561 tcatagatca cgaacagaga ggagtacaga cagcccagaa aggacaccag gctagagaaa
     1621 tacatcacgc cgttggcgga tcccacggcg cttgtcagtg gtcccagcac caggctcacc
     1681 aggatctggg ccagaaagta ctggcagctc agcagagaga tatccacgcc catgcctctc
     1741 ctggttccgt cggcggagct tcctgcgaac ttcttagact gatagtagtc gcacagcagg
     1801 gagtagggca gtgtgcacag ggtgctaaac aggatgccat atgtgatgca cagagacagc
     1861 accacgtaca gatttctgga cagtgtggcc agtccggttc ccaggccaaa ggccagatag
     1921 gcgatgaagt acagggtccg cacggacagg aactcctcca gcttctccag gatggcagaa
     1981 taaaaggcgg cggagaaggc gtagatgcac atgccccagc agcccattgt cacgccgctg
     2041 ttatacttct ggtaggcctc agaggtgtgt ggggccttag gatcgccctg aaacaccacc
     2101 tcgcccataa agtcggtata gaacagcagc atgccctcaa agctcagcca gcccaggaag
     2161 tgattcacgc acagtgtcct cagggccttt ggcatgttac agatggtgct gcacagtctg
     2221 cccacagaca gaggctgctc tgccctctca gaggatccgg tcagctcgga ctcgtacttc
     2281 acgccattca gcaggatgtt ggccacctgc tgggagaatg tcacgttgcg ccttctgctg
     2341 gtctcagggc ctcctcctcc tgctgcatct gggattgcca gtgtctgtgg gcgcttcagg
     2401 atgccgctag acctgggctt agaggtgtcc agggagccca cgcgcaggat atcgccgtca
     2461 cagccgctgg tcagggcgcc ctcccttccc tccagcagtc ctctatcctg ccgataaaag
     2521 ccgtctggtg cccttgggaa agacacggag atgggtggcc taggcacaga tcctgggatt
     2581 gccaggtagg agtcgtgtcc tcctgtaaag caatcgatca gcacggagtc gatattggct
     2641 gtgccaaagg agctggcgaa ctcgctgatg ccggtcagag aggaatcgcg ggagatgaag
     2701 ctgccgtact taggggtcag gggagaaggg ggggagattg ggctagaaaa gtttgtggcg
     2761 gtgtggctag gcagagagtc gcctggtccc tcctcaggca gcactggagg gcttggtggc
     2821 agtggcaggc taggagactt catggcggcc ctcttctcag aaggtggcct cagtggcctc
     2881 tctgggatag acaccagtgt cagcactgtg gtcacggaca gtgtcacggc ggtgaacaga
     2941 tagatcactc tcagctgtcc tcccagggcc cgtccaaatc cggtcttatc ccagtggatg
     3001 ccgcccacca cgtagccgaa gcctcctccc agtcctgcca gcagtgcgtg gatattcagg
     3061 cctctatcct ggtcggcggg gctgcacacg tccatcatgt aggcgtgaga tgggttatcg
     3121 gcggagtcgg cgctgaagtc catcagcacc actccgcaca ctgtcagcag cagtccccac
     3181 ttgtgattgc cggtcacatc tgccagggcg attccgatgt cccttccgtt cagcagcagg
     3241 ctcagtccca gcagggcgcc gattgccagc accaggataa agggccggcg cctgccgaat
     3301 ctgctggtac acctatcaga ccatgctccc agcagtggct gcagcagaaa gcccaggatt
     3361 ggggagatga accacaccag gctatacagc tggtcaggca ggcccatctg cagcagcacg
     3421 ggtgtcacat aggcggtctc catggcgtaa gaaaactcga tgccgaacag gatgcagcca
     3481 ttaaacagca gctctctgaa ggaccgctgg gggtgcagat cgccgaagtc caccagctcc
     3541 agaggacagg gggtgtttgg agggggtgga gggcttggtc tgatgcactt tctccgctta
     3601 gggtgccgct tgaagttgtt ggcgcggtgg gacaggtgcc ttgtcacgga tccgctgtat
     3661 ccggtcacct gggagcgcca aaaatcctgg ggtgccacgc ttgggaacag tgcgtcgcct
     3721 ggtggtgtgg agcttgctgc agggatcatg gttggcaggg cagggggcct gccctgaggg
     3781 tgcagcctgc atgcatccct gtcgccggag ggctgctggc ggcctggcct aggtcctggc
     3841 tgctgcagca tggtgaaggg ggcggccgcg gagcctgctt ttttgtacaa acttgtgggc
     3901 gcgcctagtt tatcgtcatc gtctttgtag tctggcgcgc cgccgccggg gccagatccc
     3961 cccttctcgg cgctccgcag gctgatctcg ccgcccatcc agggcttgat gatgccgtcc
     4021 tgctccagca gcagggcgcc ctgcttgtcg atgccccggc tgatgccgaa gatctccttg
     4081 tcgccgatga tcagcttcac gggccggttg atgaagttgt ccagcttctc ccaccggctc
     4141 aggtaggggg ccaggccctc ctgctcgaac agctccaggg cggcccgcag ctcccggatc
     4201 agcatggcgg ccagggtgtt ccggtccagg ttgatgccgg cctcctgcag ggtgatccag
     4261 ccctggttca ccacgctctc ctccacccgc cgcatggcca tgttgatgcc ggcgccgatc
     4321 acgatctggg cggcgtcgcc ggtcttgccg gtcagctcca ccaggatgcc ggccagcttc
     4381 cggtcctgca ggtacaggtc gttgggccac ttcacccgca ccttgtcggc gcccagcttc
     4441 cgcagcacct cggccatcac gatgccgatc accaggctca ggccgatggc ggcggcgggg
     4501 ccctgctcca gccgccagaa catgctcagg tacaggttgg cgccgaaggg gctgaaccac
     4561 ttccggcccc ggccgccccg gccggcctgc tggtactcgg cgatgcaggc gtcgccgctc
     4621 ttcagctcgc cgatccggtc cagcaggtac tggttggtgc tgtcgatcac gggcagcacg
     4681 gccacgctgc cgccgtccag ctggcccagg atctgcttgg cgttcagcag ctggatgggc
     4741 tcgggcaggc tgtagccctt gccgggcacg gtgaacacgt ccacgcccca gtcccgcagg
     4801 gtctggatgt gcttgttgat ggcggcccgg ctcatgccca gggtctcgcc cagctgctcg
     4861 ccgctgtgga actcgccgtt ggccagcagg gcgatcagct tcaggggcac ggtgttgtcc
     4921 ttcatggtgg cgctagctgg atccgagctc ggtaccaagc ttaagtttaa acgctagagt
     4981 ccggaggctg gatcggtccc ggtgtcttct atggaggtca aaacagcgtg gatggcgtct
     5041 ccaggcgatc tgacggttca ctaaacgagc tcgtcgacga tctctatcac tgatagggag
     5101 atctctatca ctgataggga gagctctgct tatatagacc tcccaccgta cacgcctacc
     5161 gcccatttgc gtcaatgggg cggagttgtt acgacatttt ggaaagtccc gttgattttg
     5221 gtgccaaaac aaactcccat tgacgtcaat ggggtggaga cttggaaatc cccgtgagtc
     5281 aaaccgctat ccacgcccat tgatgtactg ccaaaaccgc atcaccatgg taatagcgat
     5341 gactaatacg tagatgtact gccaagtagg aaagtcccat aaggtcatgt actgggcata
     5401 atgccaggcg ggccatttac cgtcattgac gtcaataggg ggcgtacttg gcatatgata
     5461 cacttgatgt actgccaagt gggcagttta ccgtaaatac tccacccatt gacgtcaatg
     5521 gaaagtccct attggcgtta ctatgggaac atacgtcatt attgacgtca atgggcgggg
     5581 gtcgttgggc ggtcagccag gcgggccatt taccgtaagt tatgtaacgc ggaactccat
     5641 atatgggcta tgaactaatg accccgtaat tgattactat taataactag tcaataatca
     5701 atgtcaacgc gtatatctgg cccgtacatc gcgaagcagc gcaaaacgcc taaccctaag
     5761 cagattcttc atgcaattgt cggtcaagcc ttgccttgtt gtagcttaaa ttttgctcgc
     5821 gcactactca gcgacctcca acacacaagc agggagcaga tactggctta actatgcggc
     5881 atcagagcag attgtactga gagtgcacca taggggatcg ggagatctcc cgatccgtcg
     5941 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     6001 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     6061 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     6121 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     6181 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     6241 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     6301 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     6361 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     6421 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     6481 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     6541 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     6601 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     6661 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     6721 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     6781 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     6841 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     6901 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     6961 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     7021 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     7081 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     7141 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     7201 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     7261 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     7321 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     7381 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     7441 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     7501 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     7561 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     7621 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     7681 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     7741 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     7801 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     7861 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     7921 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     7981 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
     8041 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
     8101 gattacgcca agctctagct agaggtcgac ggtatacaga catgataaga tacattgatg
     8161 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
     8221 atgctattgc tttatttgta accattataa gctgcaataa acaagttggg gtgggcgaag
     8281 aactccagca tgagatcccc gcgctggagg atcatccagc cggcgtcccg gaaaacgatt
     8341 ccgaagccca acctttcata gaaggcggcg gtggaatcga aatctcgtag tacgtgctat
     8401 tcctttgccc tcggacgagt gctggggcgt cggtttccac tatcggcgag tacttctaca
     8461 cagccatcgg tccagacggc cgcgcttctg cgggcgattt gtgtacgccc gacagtcccg
     8521 gctccggatc ggacgattgc gtcgcatcga ccctgcgccc aagctgca
//
