LOCUS       sequence-45553-e        1755 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Azpiazu I, Gautam N
  TITLE     A fluorescence resonance energy transfer-based sensor indicates that
            receptor access to a G protein is unrestricted in a living mammalian
            cell.
  JOURNAL   J Biol Chem. 2004 Jun 25;279(26):27709-18. Epub 2004 Apr 12.
  PUBMED    15078878
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2004-06-25"; volume: "279(26)"; pages: "27709-18. 
            Epub 2004 Apr 12"
COMMENT     Sequence Label: YFP B1 in pcDNA3.1
FEATURES             Location/Qualifiers
     source          1..1755
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..720
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             7..720
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(37..65)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     660..680
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 ggatccatga gtaaaggaga agaacttttc actggagttg tcccaattct tgttgaatta
       61 gatggtgatg ttaatgggca caaattttct gtcagtggag agggtgaagg tgatgcaaca
      121 tacggaaaac ttacccttaa atttatttgc actactggaa aactacctgt tccatggcca
      181 acacttgtca ctactttcgg ttatggtctt atgtgctttg caagataccc agatcatatg
      241 aaacagcatg actttttcaa gagtgccatg cccgaaggtt atgtacagga aagaactata
      301 tttttcaaag atgacgggaa ctacaagaca cgtgctgaag tcaagtttga aggtgatacc
      361 cttgttaata gaatcgagtt aaaaggtatt gattttaaag aagatggaaa cattcttgga
      421 cacaaattgg agtacaacta taactcacac aatgtataca tcatggcaga caaacaaaag
      481 aatggaatca aagttaactt caaaattaga cacaacattg aagatggaag cgttcaacta
      541 gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt accagacaac
      601 cattacctgt cctatcaatc taagctttcg aaagatccca acgaaaagag agaccacatg
      661 gtccttcttg agtttgtaac agctgctggg attacacatg gcatggatga actatacaaa
      721 actagtatga gtgaacttga ccagttacgg caggaggccg aacagcttaa aaaccaaatt
      781 agagatgctc ggaaggcgtg tgcagatgcg actctctctc agatcacaaa caacatcgac
      841 cctgtgggaa gaatccagat gcgcaccagg aggacgctga ggggacactt ggccaagatc
      901 tatgccatgc actggggcac cgactccagg cttctactca gtgcctcgca ggatggtaaa
      961 cttattatct gggacagcta caccacaaac aaggtccatg ccatccctct gcgctcctcg
     1021 tgggtcatga cgtgcgctta tgccccttct gggaactacg tggcctgtgg aggcctggat
     1081 aacatctgct ccatttacaa tctgaaaact cgtgaaggga atgtacgtgt gagtcgtgag
     1141 ctggctggac acacaggtta cctgtcctgc tgccgttttc tggatgacaa tcagattgtt
     1201 accagctctg gagacaccac ctgtgccctg tgggacatcg agactggcca gcagacgacc
     1261 acattcactg gacacaccgg agacgtcatg agcctttcac tcgctccgga caccagactg
     1321 tttgtctctg gtgcttgtga cgcttcagcc aaactctggg acgtgcgaga agggatgtgc
     1381 cggcaaacct tcaccggcca tgagtccgat atcaatgcca tctgtttctt cccgaacggc
     1441 aatgcatttg ccactggctc agacgatgcc acctgccggc tgttcgacct ccgtgcggac
     1501 caggagctca tgacctactc ccatgacaac atcatctgcg ggatcacctc cgtctccttc
     1561 tccaagagcg ggcgcctcct tctcgcaggg tacgacgact tcaactgcaa cgtctgggac
     1621 gccctcaagg ccgacagggc aggggtcttg gccgggcatg acaaccgcgt gagctgcctg
     1681 ggggtaactg atgacgggat ggccgtggcg acgggatcct gggacagctt cctcaaaatc
     1741 tggaactaat ctaga
//
