LOCUS       sequence-455551-       11199 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  All-in-one lentiviral version of the PEAR reporter. Constitutive RFP
            expression with GFP turned on in event of prime editing..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11199)
  AUTHORS   Gould SI, Wuest AN, Dong K, Johnson GA, Hsu A, Narendra VK, Atwa O, 
            Levine SS, Liu DR, Sanchez Rivera FJ
  TITLE     High-throughput evaluation of genetic variants with prime editing 
            sensor libraries.
  JOURNAL   Nat Biotechnol. 2024 Mar 12. doi: 10.1038/s41587-024-02172-9.
  PUBMED    38472508
REFERENCE   2  (bases 1 to 11199)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2024 Mar 12. doi: 10.1038/s41587-024-02172-9."
COMMENT     Sequence Label: Lenti-PEAR-mCherry
FEATURES             Location/Qualifiers
     source          1..11199
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2638..2886
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2638..2658
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2809..2828
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        2907..2982
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        3137..3347
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      3378..3387
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(4487..4506)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4834..4855
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4943..5495
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5087..5104)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5314..5333
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5507..6202
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(5640..5658)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5908..5927
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    6291..6879
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6344..6364)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6762..6773)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6951..7184
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7210..7227)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7216..7440
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7486..7914
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7573..7592)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7783..7804
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7923..7943)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7928..8257
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8108..8243
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8170..8189
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8305..8352
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8371..8745
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8875..8996
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8912..8931)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8966..8985
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9045..9061)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9045..9061)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9058..9080)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9069..9085
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9093..9099,9100..9117,9118..9123))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9138..9159
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9276..9293)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9447..10035)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9527..9546)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10206..10997,10998..11066))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10829..10848
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11067..11171)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta aggtaccgat cagtgtgagg gagtgtaaag ctggtttgag
     2641 ggcctatttc ccatgattcc ttcatatttg catatacgat acaaggctgt tagagagata
     2701 attggaatta atttgactgt aaacacaaag atattagtac aaaatacgtg acgtagaaag
     2761 taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga ctatcatatg
     2821 cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga
     2881 aacaccgagc tggacggcga cgtaaagttt tagagctaga aatagcaagt taaaataagg
     2941 ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gcggctacgt gcaggtaagt
     3001 ccgtttacgt cgccgtcctt ttttccgtta cataacttac ggtaaatggc ccaattcgag
     3061 tggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtcctaa ctcgaacgct
     3121 agctgtgcga tcgtttggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
     3181 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
     3241 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
     3301 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacagaaa ccgctgttcc
     3361 taggaatccc gaggcctgcc accatggtga gcaagggcga ggagctgttc accggggtgg
     3421 tgcccatcct ggtcgaactc gatggagatg tgaacggcca caagttcagc gtgtccggcg
     3481 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
     3541 agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca
     3601 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     3661 acgtgcagac aagtccgttt acgtcgccgt ccagctcatt ttttagttaa aatatgggaa
     3721 agtttaaaag aaggaatgat gaataaacta tacaaccact ttaaatatgt gttggtcccc
     3781 tttaagtcag caagcagata tatcagatat ggcagctaac ttaaggagtc cccgtggata
     3841 ctggatgaat agcccccaat tccaccaatg caagaaccaa cagtctattt ggtcttgatt
     3901 aggacagtaa acttcctgca caccatagtc tgtgcactaa acaggagtaa aacaccctta
     3961 aggcatctct tacattggct gcattaaagt gtgtcactta atgacatccg tgatatcagg
     4021 gatagtttga cattggacat acagttctct ctcagtgatt gtcacctgct tatatagtac
     4081 gaggacccat atgtggtcaa tcactttctt gttgccactc catgccaggg aagacatagt
     4141 tacaatgagt tataattata aaagctcagc cttgcatgag gatataaaat acatttgcca
     4201 tacctctaca tatgccttag ttgcttttaa gctttgtttg gaattgcaaa ggccaacttc
     4261 ctaaacacag tttcaaagta ttcttttgtc acacctttcc actggatact tgtattgtct
     4321 gcaggaagag gccaccctgc ctatgcttct ccttagggcc tcgttctgca gagtcagcat
     4381 aaactcccag tctgctcaac caattctgtc tgattagggc catatctcct taccatgagt
     4441 tgatttttgt ttcttttttg agcaggagcg caccatcttc ttcaaggacg acggcaacta
     4501 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     4561 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     4621 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     4681 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     4741 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
     4801 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     4861 cgccgggatc actctcggca tggacgagct gtacaagtaa gacccgacat tagcgctaca
     4921 gcttaagcgg cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt
     4981 gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg
     5041 aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga
     5101 atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa
     5161 acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc
     5221 tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac
     5281 gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag
     5341 gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc
     5401 acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg
     5461 gacgtggttt tcctttgaaa aacacgatga taatatggcc acaaccatgg tgagcaaggg
     5521 cgaggcagtg atcaaggagt tcatgcggtt caaggtgcac atggagggct ccatgaacgg
     5581 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc
     5641 caagctgaag gtgaccaagg gtggccccct gcccttctcc tgggacatcc tgtcccctca
     5701 gttcatgtac ggctccaggg ccttcaccaa gcaccccgcc gacatccccg actactataa
     5761 gcagtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgc
     5821 cgtgaccgtg acccaggaca cctccctgga ggacggcacc ctgatctaca aggtgaagct
     5881 ccgcggcacc aacttccctc ctgacggccc cgtaatgcag aagaagacaa tgggctggga
     5941 agcgtccacc gagcggttgt accccgagga cggcgtgctg aagggcgaca ttaagatggc
     6001 cctgcgcctg aaggacggcg gccgctacct ggcggacttc aagaccacct acaaggccaa
     6061 gaagcccgtg cagatgcccg gcgcctacaa cgtcgaccgc aagttggaca tcacctccca
     6121 caacgaggac tacaccgtgg tggaacagta cgaacgctcc gagggccgcc actccaccgg
     6181 cggcatggac gagctgtaca agtaaaaacg ttgttgtttg gggttgaatt actctacatg
     6241 tttaagggtt ccggttccac taggtacaat tcgatatcaa gcttatcgat aatcaacctc
     6301 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     6361 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     6421 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     6481 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     6541 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     6601 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     6661 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     6721 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     6781 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     6841 ctcagacgag tcggatctcc ctttgggccg cctccccgcg tcgactttaa gaccaatgac
     6901 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     6961 aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga
     7021 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     7081 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     7141 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagggccc ggcgaaaccc
     7201 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     7261 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     7321 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     7381 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     7441 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
     7501 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     7561 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     7621 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     7681 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     7741 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     7801 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     7861 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct
     7921 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     7981 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     8041 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     8101 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     8161 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
     8221 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
     8281 cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata
     8341 gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc cgttccggtg
     8401 ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc
     8461 cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc
     8521 agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc
     8581 ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc
     8641 gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac
     8701 ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt gctacgagat
     8761 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
     8821 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg
     8881 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     8941 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     9001 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
     9061 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     9121 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     9181 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     9241 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     9301 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     9361 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     9421 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     9481 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     9541 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     9601 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     9661 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     9721 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     9781 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     9841 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     9901 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     9961 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
    10021 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
    10081 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
    10141 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
    10201 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
    10261 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
    10321 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
    10381 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
    10441 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
    10501 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
    10561 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
    10621 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
    10681 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
    10741 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
    10801 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
    10861 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
    10921 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
    10981 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
    11041 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
    11101 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
    11161 ggggttccgc gcacatttcc ccgaaaagtg ccacctgac
//
