LOCUS       sequence-455585-        1208 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  KHC065 (pBABE pU6 BRD2 K1-2C), gRNA and cas9D10A mammalian 
            expression vector for CRISPR mediated cutting of BRD2 at the 
            N-terminus, use with KHC064.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Bond AG, Craigon C, Chan KH, Testa A, Karapetsas A, Fasimoye R, 
            Macartney T, Blow JJ, Alessi DR, Ciulli A
  TITLE     Development of BromoTag: A ""Bump-and-Hole""-PROTAC System to Induce
            Potent, Rapid, and Selective Degradation of Tagged Target Proteins.
  JOURNAL   J Med Chem. 2021 Oct 28;64(20):15477-15502. doi: 
            10.1021/acs.jmedchem.1c01532. Epub 2021 Oct 15.
  PUBMED    34652918
REFERENCE   2  (bases 1 to 1208)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.1c01532"; journalName: "J Med Chem"; date: 
            "2021-10-28- 28"; volume: "64"; issue: "20"; pages: "15477-15502"
COMMENT     Sequence Label: KHC065 pBABE pU6 BRD2 K1-2C
FEATURES             Location/Qualifiers
     source          1..1208
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        64..139
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             complement(170..586)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(186..202)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(218..240)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    787..986
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
ORIGIN
        1 gatttcttgg ctttatatat cttgtggaaa ggacgaaaca ccgtcagccg cggaaagtcc
       61 ggggttttag agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa
      121 agtggcaccg agtcggtgct tttttgaatt cgcggccgca gatccggccg gcgcctagag
      181 aaggagtgag ggctggataa agggaggatc gaggcggggt cgaacgagga ggttcaaggg
      241 ggagagacgg ggcggatgga ggaagaggag gcggaggctt agggtgtaca aagggcttga
      301 cccagggagg ggggtcaaaa gccaaggctt cccaggtcac gatgtagggg acctggtctg
      361 ggtgtccatg cgggccaggt gaaaagacct tgatcttaac ctgggtgatg aggtctcggt
      421 taaaggtgcc gtctcgcggc catccgacgt taaaggttgg ccattctgca gagcagaagg
      481 taacccaacg tctcttcttg acatctaccg actggttgtg agcgatccgc tcgacatctt
      541 tccagtgacc taaggtcaaa cttaagggag tggtaacagt ctggccctaa ttttcagaca
      601 aatacagaaa cacagtcaga cagagacaac acagaacgat gctgcagcgc tgcagcagac
      661 aagacgcgcg gcttcggttc caaaccgaaa gcaaaaattc agacggaggc gggaactgtt
      721 ttaggttctc gtctcctacc agaaccacat atcctgacgg ggtcggattc cacatcgact
      781 cccttcctca ggtcgggcca caaaaacggc ccccaaagtc cctgggacgt ctcccagggt
      841 tgcggccggg tgttcagaac tcgtcagttc caccacgggt ccgccagata cagagctagt
      901 tagctaacta gtaccgacgc aggcgcataa aatcagtcat agacactaga caatcggaca
      961 gacacagata agttgctggc cagcttgcct cccggtggtg ggtcggtggt ccctgggcag
     1021 gggtctcccg atcccggacg agcccccaaa tgaaagaccc ccgctgacgg gtagtcaatc
     1081 actcagagga gaccctccca aggaacagcg agaccacaag tcggatgcaa ctgcagaagg
     1141 gttattggaa acccgggtac ccgggcgaat cagtcaatcg gaggaatggg cccccaataa
     1201 nagggttt
//
