LOCUS       sequence-455586-        1231 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR mediated KI of BromoTag at N-terminus of 
            BRD2-GFP-P2A-BromoTag, works well with KHC064 and KHC065.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Bond AG, Craigon C, Chan KH, Testa A, Karapetsas A, Fasimoye R, 
            Macartney T, Blow JJ, Alessi DR, Ciulli A
  TITLE     Development of BromoTag: A ""Bump-and-Hole""-PROTAC System to Induce
            Potent, Rapid, and Selective Degradation of Tagged Target Proteins.
  JOURNAL   J Med Chem. 2021 Oct 28;64(20):15477-15502. doi: 
            10.1021/acs.jmedchem.1c01532. Epub 2021 Oct 15.
  PUBMED    34652918
REFERENCE   2  (bases 1 to 1231)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.1c01532"; journalName: "J Med Chem"; date: 
            "2021-10-28- 28"; volume: "64"; issue: "20"; pages: "15477-15502"
COMMENT     Sequence Label: KHC068 BRD2-KI-GFP-2A-L387A
FEATURES             Location/Qualifiers
     source          1..1231
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             24..35
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             join(552..554,555..557,558..1070)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(597..618)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(858..877)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ggcaaaaatc ctattaaggg cgaattgaag gaaggccgtc aaggccgcat ccgcccaatc
       61 ctcggagtct gtccaccccc tctactccgc cctcaagagg atttcaaaga tggaggcggc
      121 ggctccctaa accacttttc gtgttcatcc gcctccatcc gagatcgaaa cgggacctcg
      181 tcggccccgt aggggcccga caagaagagg gaatccctgc agaccaacag cgggctatat
      241 tgacgacggt gtctgagatc ggggaccgtc ttttgaagag tcagtccctc cttagttgcc
      301 cgcctcagct gaggccgccg ccattttctt gctgtccgcc gtctgcagag cgcgccaagc
      361 tgcccggagc tctccgagag gccccaaaga gactgctttc gtgccggcca ggcagggggt
      421 ttgtcgcctg gaggcccaag aggaacggcc tccccccaac ttagcgggtt atgctggacc
      481 gggcggtgag gggaaccgag ggcacccgga ctttccgcgg ctgagggcag cgccggttcg
      541 ttgcggtcaa gatggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
      601 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
      661 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
      721 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg
      781 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
      841 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
      901 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
      961 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca
     1021 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacgggagcg
     1081 tgcagctcgc cgaaccctac cagcagaaac cccccatcgg ggaagggccc ggggtggtgg
     1141 ccgaaaaccc taactggaaa ncccagtccg ccctgnanca agaaccccaa ggaaaagcgg
     1201 gatcccttgg cccggtgggg attcgggacc g
//
