LOCUS       sequence-455588-       11521 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mapping of cytosol-facing chloroplast outer membrane proximity 
            proteome by proximity-dependent biotinylation in living Arabidopsis 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11521)
  AUTHORS   Bao X, Jia H, Zhang X, Tian S, Zhao Y, Li X, Lin P, Ma C, Wang P, 
            Song CP, Zhu X
  TITLE     Mapping of cytosol-facing organelle outer membrane proximity 
            proteome by proximity-dependent biotinylation in living Arabidopsis 
            cells.
  JOURNAL   Plant J. 2024 Apr;118(1):7-23. doi: 10.1111/tpj.16641. Epub 2024 Jan
            23.
  PUBMED    38261530
REFERENCE   2  (bases 1 to 11521)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.16641";
            journalName: "Plant J"; date: "2024-04"; volume: "118"; issue: "1"; 
            pages: "7-23"
COMMENT     Sequence Label: CP3
FEATURES             Location/Qualifiers
     source          1..11521
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(43..64)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(join(199..711,712..714,715..717))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     392..411
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             complement(736..1506)
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     promoter        complement(1629..2009)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     primer_bind     complement(2543..2560)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2543..2559)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2552..2574)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    2762..2786
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4087..4716
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5145..6218
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      6284..6478
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(6714..6736)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    6822..6962
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     6877..6896
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6977..6994)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7148..7736)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7228..7247)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7823..8617)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    9042..9066
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    9144..9318
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(9359..10384)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(10457..10802)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(10494..10514)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    10993..11014
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11029..11034,11035..11052,11053..11059)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11067..11083
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11072..11094
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11091..11107
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11091..11107
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      complement(11124..11376)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(11396..11422)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(11435..11461)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(11474..11500)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 cttgtacagg tcgtccatgc cgagagtgat cccggcggcg gtcacgaact ccagcaggac
       61 catgtgatcg cgcttctcgt tggggtcttt gctcagggcg gactgggtgc tcaggtagtg
      121 gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc
      181 gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg aagttcacct tgatgccgtt
      241 cttctgcttg tcggccatga tatagacgtt gtggctgttg tagttgtact ccagcttgtg
      301 ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc ggttcaccag
      361 ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt ccttgaagaa
      421 gatggtgcgc tcctggacgt agccttcggg catggcggac ttgaagaagt cgtgctgctt
      481 catgtggtcg gggtagcggc tgaagcactg cacgccgtag gtcagggtgg tcacgagggt
      541 gggccagggc acgggcagct tgccggtggt gcagatgaac ttcagggtca gcttgccgta
      601 ggtggcatcg ccctcgccct cgccggacac gctgaacttg tggccgttta cgtcgccgtc
      661 cagctcgacc aggatgggca ccaccccggt gaacagctcc tcgcccttgc tcaccatggt
      721 accggatcca ctagtctttt cggcagaccg cagactgatt tctccgccca tccagggttt
      781 gataactccg tcctgttcca gcagcagggc tccctgttta tcaatccccc ggctaatccc
      841 gaaaatctct ttgtccccaa tgatcagctt cacgggtcta ttgatgaagt tatccagctt
      901 ctcccaccgt gacagatatg gagccaggcc ttcctgctcg aacagttcca gggctgcccg
      961 cagctctcgg atcagcatag cggccagagt attcctgtcc aggttaatcc ctgcttcctg
     1021 cagtgtgatc cagccctgat tgaccacgct ttcctccacg cgcctcatag ccacgttaat
     1081 cccggcgcca atgacgatct gtgcagcatc gcctgttatt ccggccagct ccaccaggat
     1141 gcctgccagc tttctatcct gcagatacag gtcattgggc catttgactc gcaccttgtc
     1201 tgctcccagc tttcgcagcg cttctgccat gacaattccg atgaccgggc ccaggccgat
     1261 tgctgctggt ccccgcttca ggcgccaaaa catactcagg tacaggttag ctccaaaagg
     1321 agagaaccat ttcctccctc tgcttcctct ccctgcctgc tgatattctg caatgcaagc
     1381 atccccactc ttcagctcgc cgattcgatc cagcaggtac tgattggtgg agtcgaccac
     1441 aggcaggact gccacgctcc cgccgtccag ctgtcccaga atctgtttag cgttcagcag
     1501 cgggatcata ctagtatctt cacggacggt cttcttcagc tttttggtaa gcataagaat
     1561 cccagcgaga cctaaaccta gaagaaccca gagattcgca gcttgagacg ccataagctt
     1621 tctagagtcg atttggtgta tcgagattgg ttatgaaatt cagatgctag tgtaatgtat
     1681 tggtaatttg ggaagatata ataggaagca aggctattta tccatttctg aaaaggcgaa
     1741 atggcgtcac cgcgagcgtc acgcgcattc cgttcttgct gtaaagcgtt gtttggtaca
     1801 cttttgacta gcgaggcttg gcgtgtcagc gtatctattc aaaagtcgtt aatggctgcg
     1861 gatcaagaaa aagttggaat agaaacagaa tacccgcgaa attcaggccc ggttgccatg
     1921 tcctacacgc cgaaataaac gaccaaatta gtagaaaaat aaaaactgac tcggatactt
     1981 acgtcacgtc ttgcgcactg atttgaaaaa tctccctcga tcgagaaaga gatcaatgtt
     2041 gagctgcttc aaaagcaatg ggattgacca gctcgcggat cctacaggcc aaattcgctc
     2101 ttagccgtac aatattactc accggtgcga tgccccccat cgtaggtgaa ggtggaaatt
     2161 aatgatccat cttgagacca caggcccaca acagctacca gtttcctcaa gggtccacca
     2221 aaaacgtaag cgcttacgta catggtcgat aagaaaaggc aatttgtaga tgttaacatc
     2281 caacgtcgct ttcagggatc ctacaggcca aattcgctct tagccgtaca atattactca
     2341 ccggtgcgat gccccccatc gtaggtgaag gtggaaatta atgatccatc ttgagaccac
     2401 aggcccacaa cagctaccag tttcctcaag ggtccaccaa aaacgtaagc gcttacgtac
     2461 atggtcgata agaaaaggca atttgtagat gttaacatcc aacgtcgctt tcagggatcc
     2521 gcgagcttat cgataccgtg gcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     2581 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     2641 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgct
     2701 agagcagctt gagcttggat cagattgtcg tttcccgcct tcagtttaaa ctatcagtgt
     2761 ttgacaggat atattggcgg gtaaacctaa gagaaaagag cgtttattag aataatcgga
     2821 tatttaaaag ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtgca tgccaaccac
     2881 agggttcccc tcgggatcaa agtactttga tccaacccct ccgctgctat agtgcagtcg
     2941 gcttctgacg ttcagtgcag ccgtcttctg aaaacgacat gtcgcacaag tcctaagtta
     3001 cgcgacaggc tgccgccctg cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca
     3061 taaagtagaa tacttgcgac tagaaccgga gacattacgc catgaacaag agcgccgccg
     3121 ctggcctgct gggctatgcc cgcgtcagca ccgacgacca ggacttgacc aaccaacggg
     3181 ccgaactgca cgcggccggc tgcaccaagc tgttttccga gaagatcacc ggcaccaggc
     3241 gcgaccgccc ggagctggcc aggatgcttg accacctacg ccctggcgac gttgtgacag
     3301 tgaccaggct agaccgcctg gcccgcagca cccgcgacct actggacatt gccgagcgca
     3361 tccaggaggc cggcgcgggc ctgcgtagcc tggcagagcc gtgggccgac accaccacgc
     3421 cggccggccg catggtgttg accgtgttcg ccggcattgc cgagttcgag cgttccctaa
     3481 tcatcgaccg cacccggagc gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc
     3541 cccgccctac cctcaccccg gcacagatcg cgcacgcccg cgagctgatc gaccaggaag
     3601 gccgcaccgt gaaagaggcg gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg
     3661 cacttgagcg cagcgaggaa gtgacgccca ccgaggccag gcggcgcggt gccttccgtg
     3721 aggacgcatt gaccgaggcc gacgccctgg cggccgccga gaatgaacgc caagaggaac
     3781 aagcatgaaa ccgcaccagg acggccagga cgaaccgttt ttcattaccg aagagatcga
     3841 ggcggagatg atcgcggccg ggtacgtgtt cgagccgccc gcgcacgtct caaccgtgcg
     3901 gctgcatgaa atcctggccg gtttgtctga tgccaagctg gcggcctggc cggccagctt
     3961 ggccgctgaa gaaaccgagc gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag
     4021 cttgcgtcat gcggtcgctg cgtatatgat gcgatgagta aataaacaaa tacgcaaggg
     4081 gaacgcatga aggttatcgc tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc
     4141 gcaacccatc tagcccgcgc cctgcaactc gccggggccg atgttctgtt agtcgattcc
     4201 gatccccagg gcagtgcccg cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt
     4261 gtcggcatcg accgcccgac gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc
     4321 gtagtgatcg acggagcgcc ccaggcggcg gacttggctg tgtccgcgat caaggcagcc
     4381 gacttcgtgc tgattccggt gcagccaagc ccttacgaca tatgggccac cgccgacctg
     4441 gtggagctgg ttaagcagcg cattgaggtc acggatggaa ggctacaagc ggcctttgtc
     4501 gtgtcgcggg cgatcaaagg cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg
     4561 tacgagctgc ccattcttga gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc
     4621 gccgccggca caaccgttct tgaatcagaa cccgagggcg acgctgcccg cgaggtccag
     4681 gcgctggccg ctgaaattaa atcaaaactc atttgagtta atgaggtaaa gagaaaatga
     4741 gcaaaagcac aaacacgcta agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa
     4801 cgttggccag cctggcagac acgccagcca tgaagcgggt caactttcag ttgccggcgg
     4861 aggatcacac caagctgaag atgtacgcgg tacgccaagg caagaccatt accgagctgc
     4921 tatctgaata catcgcgcag ctaccagagt aaatgagcaa atgaataaat gagtagatga
     4981 attttagcgg ctaaaggagg cggcatggaa aatcaagaac aaccaggcac cgacgccgtg
     5041 gaatgcccca tgtgtggagg aacgggcggt tggccaggcg taagcggctg ggttgcctgc
     5101 cggccctgca atggcactgg aacccccaag cccgaggaat cggcgtgagc ggtcgcaaac
     5161 catccggccc ggtacaaatc ggcgcggcgc tgggtgatga cctggtggag aagttgaagg
     5221 ccgcgcaggc cgcccagcgg caacgcatcg aggcagaagc acgccccggt gaatcgtggc
     5281 aagcggccgc tgatcgaatc cgcaaagaat cccggcaacc gccggcagcc ggtgcgccgt
     5341 cgattaggaa gccgcccaag ggcgacgagc aaccagattt tttcgttccg atgctctatg
     5401 acgtgggcac ccgcgatagt cgcagcatca tggacgtggc cgttttccgt ctgtcgaagc
     5461 gtgaccgacg agctggcgag gtgatccgct acgagcttcc agacgggcac gtagaggttt
     5521 ccgcagggcc ggccggcatg gccagtgtgt gggattacga cctggtactg atggcggttt
     5581 cccatctaac cgaatccatg aaccgatacc gggaagggaa gggagacaag cccggccgcg
     5641 tgttccgtcc acacgttgcg gacgtactca agttctgccg gcgagccgat ggcggaaagc
     5701 agaaagacga cctggtagaa acctgcattc ggttaaacac cacgcacgtt gccatgcagc
     5761 gtacgaagaa ggccaagaac ggccgcctgg tgacggtatc cgagggtgaa gccttgatta
     5821 gccgctacaa gatcgtaaag agcgaaaccg ggcggccgga gtacatcgag atcgagctag
     5881 ctgattggat gtaccgcgag atcacagaag gcaagaaccc ggacgtgctg acggttcacc
     5941 ccgattactt tttgatcgat cccggcatcg gccgttttct ctaccgcctg gcacgccgcg
     6001 ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat ctacgaacgc agtggcagcg
     6061 ccggagagtt caagaagttc tgtttcaccg tgcgcaagct gatcgggtca aatgacctgc
     6121 cggagtacga tttgaaggag gaggcggggc aggctggccc gatcctagtc atgcgctacc
     6181 gcaacctgat cgagggcgaa gcatccgccg gttcctaatg tacggagcag atgctagggc
     6241 aaattgccct agcaggggaa aaaggtcgaa aaggtctctt tcctgtggat agcacgtaca
     6301 ttgggaaccc aaagccgtac attgggaacc ggaacccgta cattgggaac ccaaagccgt
     6361 acattgggaa ccggtcacac atgtaagtga ctgatataaa agagaaaaaa ggcgattttt
     6421 ccgcctaaaa ctctttaaaa cttattaaaa ctcttaaaac ccgcctggcc tgtgcataac
     6481 tgtctggcca gcgcacagcc gaagagctgc aaaaagcgcc tacccttcgg tcgctgcgct
     6541 ccctacgccc cgccgcttcg cgtcggccta tcgcggccgc tggccgctca aaaatggctg
     6601 gcctacggcc aggcaatcta ccagggcgcg gacaagccgc gccgtcgcca ctcgaccgcc
     6661 ggcgcccaca tcaaggcacc ctgcctcgcg cgtttcggtg atgacggtga aaacctctga
     6721 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
     6781 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca
     6841 cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag attgtactga
     6901 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
     6961 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     7021 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     7081 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     7141 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     7201 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     7261 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     7321 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     7381 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     7441 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     7501 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     7561 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     7621 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     7681 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     7741 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     7801 ttttggtcat gcattctagg tactaaaaca attcatccag taaaatataa tattttattt
     7861 tctcccaatc aggcttgatc cccagtaagt caaaaaatag ctcgacatac tgttcttccc
     7921 cgatatcctc cctgatcgac cggacgcaga aggcaatgtc ataccacttg tccgccctgc
     7981 cgcttctccc aagatcaata aagccactta ctttgccatc tttcacaaag atgttgctgt
     8041 ctcccaggtc gccgtgggaa aagacaagtt cctcttcggg cttttccgtc tttaaaaaat
     8101 catacagctc gcgcggatct ttaaatggag tgtcttcttc ccagttttcg caatccacat
     8161 cggccagatc gttattcagt aagtaatcca attcggctaa gcggctgtct aagctattcg
     8221 tatagggaca atccgatatg tcgatggagt gaaagagcct gatgcactcc gcatacagct
     8281 cgataatctt ttcagggctt tgttcatctt catactcttc cgagcaaagg acgccatcgg
     8341 cctcactcat gagcagattg ctccagccat catgccgttc aaagtgcagg acctttggaa
     8401 caggcagctt tccttccagc catagcatca tgtccttttc ccgttccaca tcataggtgg
     8461 tccctttata ccggctgtcc gtcattttta aatataggtt ttcattttct cccaccagct
     8521 tatatacctt agcaggagac attccttccg tatcttttac gcagcggtat ttttcgatca
     8581 gttttttcaa ttccggtgat attctcattt tagccattta ttatttcctt cctcttttct
     8641 acagtattta aagatacccc aagaagctaa ttataacaag acgaactcca attcactgtt
     8701 ccttgcattc taaaacctta aataccagaa aacagctttt tcaaagttgt tttcaaagtt
     8761 ggcgtataac atagtatcga cggagccgat tttgaaaccg cggtgatcac aggcagcaac
     8821 gctctgtcat cgttacaatc aacatgctac cctccgcgag atcatccgtg tttcaaaccc
     8881 ggcagcttag ttgccgttct tccgaatagc atcggtaaca tgagcaaagt ctgccgcctt
     8941 acaacggctc tcccgctgac gccgtcccgg actgatgggc tgcctgtatc gagtggtgat
     9001 tttgtgccga gctgccggtc ggggagctgt tggctggctg gtggcaggat atattgtggt
     9061 gtaaacaaat tgacgcttag acaacttaat aacacattgc ggacgttttt aatgtactga
     9121 attaacgccg aattaattcg ggggatctgg attttagtac tggattttgg ttttaggaat
     9181 tagaaatttt attgatagaa gtattttaca aatacaaata catactaagg gtttcttata
     9241 tgctcaacac atgagcgaaa ccctatagga accctaattc ccttatctgg gaactactca
     9301 cacattatta tggagaaact cgagcttgtc gatcgacaga tcccggtcgg catctactct
     9361 atttctttgc cctcggacga gtgctggggc gtcggtttcc actatcggcg agtacttcta
     9421 cacagccatc ggtccagacg gccgcgcttc tgcgggcgat ttgtgtacgc ccgacagtcc
     9481 cggctccgga tcggacgatt gcgtcgcatc gaccctgcgc ccaagctgca tcatcgaaat
     9541 tgccgtcaac caagctctga tagagttggt caagaccaat gcggagcata tacgcccgga
     9601 gtcgtggcga tcctgcaagc tccggatgcc tccgctcgaa gtagcgcgtc tgctgctcca
     9661 tacaagccaa ccacggcctc cagaagaaga tgttggcgac ctcgtattgg gaatccccga
     9721 acatcgcctc gctccagtca atgaccgctg ttatgcggcc attgtccgtc aggacattgt
     9781 tggagccgaa atccgcgtgc acgaggtgcc ggacttcggg gcagtcctcg gcccaaagca
     9841 tcagctcatc gagagcctgc gcgacggacg cactgacggt gtcgtccatc acagtttgcc
     9901 agtgatacac atggggatca gcaatcgcgc atatgaaatc acgccatgta gtgtattgac
     9961 cgattccttg cggtccgaat gggccgaacc cgctcgtctg gctaagatcg gccgcagcga
    10021 tcgcatccat agcctccgcg accggttgta gaacagcggg cagttcggtt tcaggcaggt
    10081 cttgcaacgt gacaccctgt gaacggcggg agatgcaata ggtcaggctc tcgctaaact
    10141 ccccaatgtc aagcacttcc ggaatcggga gcgcggccga tgcaaagtgc cgataaacat
    10201 aacgatcttt gtagaaacca tcggcgcagc tatttacccg caggacatat ccacgccctc
    10261 ctacatcgaa gctgaaagca cgagattctt cgccctccga gagctgcatc aggtcggaga
    10321 cgctgtcgaa cttttcgatc agaaacttct cgacagacgt cgcggtgagt tcaggctttt
    10381 tcatatctca ttgccccccg gatctgcgaa agctcgagag agatagattt gtagagagag
    10441 actggtgatt tcagcgtgtc ctctccaaat gaaatgaact tccttatata gaggaagggt
    10501 cttgcgaagg atagtgggat tgtgcgtcat cccttacgtc agtggagata tcacatcaat
    10561 ccacttgctt tgaagacgtg gttggaacgt cttctttttc cacgatgctc ctcgtgggtg
    10621 ggggtccatc tttgggacca ctgtcggcag aggcatcttg aacgatagcc tttcctttat
    10681 cgcaatgatg gcatttgtag gtgccacctt ccttttctac tgtccttttg atgaagtgac
    10741 agatagctgg gcaatggaat ccgaggaggt ttcccgatat taccctttgt tgaaaagtct
    10801 caatagccct ttggtcttct gagactgtat ctttgatatt cttggagtag acgagagtgt
    10861 cgtgctccac catgttggca agctgctcta gccaatacgc aaaccgcctc tccccgcgcg
    10921 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
    10981 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
    11041 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
    11101 ctatgacatg attacgaatt cccgatctag taacatagat gacaccgcgc gcgataattt
    11161 atcctagttt gcgcgctata ttttgttttc tatcgcgtat taaatgtata attgcgggac
    11221 tctaatcata aaaacccatc tcataaataa cgtcatgcat tacatgttaa ttattacatg
    11281 cttaacgtaa ttcaacagaa attatatgat aatcatcgca agaccggcaa caggattcaa
    11341 tcttaagaaa ctttattgcc aaatgtttga acgatcgggg aaattcgagc tctcaagcat
    11401 aatcaggcac atcataaggg tatccggcgc tgccagcgta gtcaggcaca tcataggggt
    11461 aggatcctgt gccggcgtaa tcaggcacgt catagggata catgccgctc cctccgagct
    11521 c
//
