LOCUS       sequence-455589-       11556 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mapping of cytosol-facing mitochondrion outer membrane proximity 
            proteome by proximity-dependent biotinylation in living Arabidopsis 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11556)
  AUTHORS   Bao X, Jia H, Zhang X, Tian S, Zhao Y, Li X, Lin P, Ma C, Wang P, 
            Song CP, Zhu X
  TITLE     Mapping of cytosol-facing organelle outer membrane proximity 
            proteome by proximity-dependent biotinylation in living Arabidopsis 
            cells.
  JOURNAL   Plant J. 2024 Apr;118(1):7-23. doi: 10.1111/tpj.16641. Epub 2024 Jan
            23.
  PUBMED    38261530
REFERENCE   2  (bases 1 to 11556)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.16641";
            journalName: "Plant J"; date: "2024-04"; volume: "118"; issue: "1"; 
            pages: "7-23"
COMMENT     Sequence Label: MT4
FEATURES             Location/Qualifiers
     source          1..11556
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(126..836,837..839,840..842))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(126..836,837..839,840..842))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(168..189)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     517..536
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             complement(849..1619)
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             complement(1635..1661)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(1674..1700)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(1713..1739)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(1792..2172)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     primer_bind     complement(2706..2723)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2706..2722)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2715..2737)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    2925..2949
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4250..4879
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5308..6381
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      6447..6641
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(6877..6899)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    6985..7125
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7040..7059
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7140..7157)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7311..7899)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7391..7410)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7986..8780)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    9205..9229
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    9307..9481
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(9522..10547)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(10620..10965)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(10657..10677)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    11156..11177
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11192..11197,11198..11215,11216..11222)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11230..11246
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11235..11257
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11254..11270
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11254..11270
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      complement(11287..11539)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
ORIGIN
        1 cagagctgct ttcggtatct cacgaaggcc caaactgcta ccagggtaag ggcaaacact
       61 ggcagcagca ccacagctac aggaactcca ctaggctctc cgtcgcctcg atgccgcggg
      121 agctccttgt acagctcgtc catgccgaga gtgatcccgg cggcggtcac gaactccagc
      181 aggaccatgt gatcgcgctt ctcgttgggg tctttgctca gggcggactg ggtgctcagg
      241 tagtggttgt cgggcagcag cacggggccg tcgccgatgg gggtgttctg ctggtagtgg
      301 tcggcgagct gcacgctgcc gtcctcgatg ttgtggcgga tcttgaagtt caccttgatg
      361 ccgttcttct gcttgtcggc catgatatag acgttgtggc tgttgtagtt gtactccagc
      421 ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc ccttcagctc gatgcggttc
      481 accagggtgt cgccctcgaa cttcacctcg gcgcgggtct tgtagttgcc gtcgtccttg
      541 aagaagatgg tgcgctcctg gacgtagcct tcgggcatgg cggacttgaa gaagtcgtgc
      601 tgcttcatgt ggtcggggta gcggctgaag cactgcacgc cgtaggtcag ggtggtcacg
      661 agggtgggcc agggcacggg cagcttgccg gtggtgcaga tgaacttcag ggtcagcttg
      721 ccgtaggtgg catcgccctc gccctcgccg gacacgctga acttgtggcc gtttacgtcg
      781 ccgtccagct cgaccaggat gggcaccacc ccggtgaaca gctcctcgcc cttgctcacc
      841 atactagtct tttcggcaga ccgcagactg atttctccgc ccatccaggg tttgataact
      901 ccgtcctgtt ccagcagcag ggctccctgt ttatcaatcc cccggctaat cccgaaaatc
      961 tctttgtccc caatgatcag cttcacgggt ctattgatga agttatccag cttctcccac
     1021 cgtgacagat atggagccag gccttcctgc tcgaacagtt ccagggctgc ccgcagctct
     1081 cggatcagca tagcggccag agtattcctg tccaggttaa tccctgcttc ctgcagtgtg
     1141 atccagccct gattgaccac gctttcctcc acgcgcctca tagccacgtt aatcccggcg
     1201 ccaatgacga tctgtgcagc atcgcctgtt attccggcca gctccaccag gatgcctgcc
     1261 agctttctat cctgcagata caggtcattg ggccatttga ctcgcacctt gtctgctccc
     1321 agctttcgca gcgcttctgc catgacaatt ccgatgaccg ggcccaggcc gattgctgct
     1381 ggtccccgct tcaggcgcca aaacatactc aggtacaggt tagctccaaa aggagagaac
     1441 catttcctcc ctctgcttcc tctccctgcc tgctgatatt ctgcaatgca agcatcccca
     1501 ctcttcagct cgccgattcg atccagcagg tactgattgg tggagtcgac cacaggcagg
     1561 actgccacgc tcccgccgtc cagctgtccc agaatctgtt tagcgttcag cagcgggatc
     1621 atgccgctcc ctccagcata atcaggcaca tcataagggt atccggcgct gccagcgtag
     1681 tcaggcacat cataggggta ggatcctgtg ccggcgtaat caggcacgtc atagggatac
     1741 atactagtat ttaaatgtcg accccgggcc cctgcagaag ctttctagag tcgatttggt
     1801 gtatcgagat tggttatgaa attcagatgc tagtgtaatg tattggtaat ttgggaagat
     1861 ataataggaa gcaaggctat ttatccattt ctgaaaaggc gaaatggcgt caccgcgagc
     1921 gtcacgcgca ttccgttctt gctgtaaagc gttgtttggt acacttttga ctagcgaggc
     1981 ttggcgtgtc agcgtatcta ttcaaaagtc gttaatggct gcggatcaag aaaaagttgg
     2041 aatagaaaca gaatacccgc gaaattcagg cccggttgcc atgtcctaca cgccgaaata
     2101 aacgaccaaa ttagtagaaa aataaaaact gactcggata cttacgtcac gtcttgcgca
     2161 ctgatttgaa aaatctccct cgatcgagaa agagatcaat gttgagctgc ttcaaaagca
     2221 atgggattga ccagctcgcg gatcctacag gccaaattcg ctcttagccg tacaatatta
     2281 ctcaccggtg cgatgccccc catcgtaggt gaaggtggaa attaatgatc catcttgaga
     2341 ccacaggccc acaacagcta ccagtttcct caagggtcca ccaaaaacgt aagcgcttac
     2401 gtacatggtc gataagaaaa ggcaatttgt agatgttaac atccaacgtc gctttcaggg
     2461 atcctacagg ccaaattcgc tcttagccgt acaatattac tcaccggtgc gatgcccccc
     2521 atcgtaggtg aaggtggaaa ttaatgatcc atcttgagac cacaggccca caacagctac
     2581 cagtttcctc aagggtccac caaaaacgta agcgcttacg tacatggtcg ataagaaaag
     2641 gcaatttgta gatgttaaca tccaacgtcg ctttcaggga tccgcgagct tatcgatacc
     2701 gtggcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     2761 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     2821 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gctagagcag cttgagcttg
     2881 gatcagattg tcgtttcccg ccttcagttt aaactatcag tgtttgacag gatatattgg
     2941 cgggtaaacc taagagaaaa gagcgtttat tagaataatc ggatatttaa aagggcgtga
     3001 aaaggtttat ccgttcgtcc atttgtatgt gcatgccaac cacagggttc ccctcgggat
     3061 caaagtactt tgatccaacc cctccgctgc tatagtgcag tcggcttctg acgttcagtg
     3121 cagccgtctt ctgaaaacga catgtcgcac aagtcctaag ttacgcgaca ggctgccgcc
     3181 ctgccctttt cctggcgttt tcttgtcgcg tgttttagtc gcataaagta gaatacttgc
     3241 gactagaacc ggagacatta cgccatgaac aagagcgccg ccgctggcct gctgggctat
     3301 gcccgcgtca gcaccgacga ccaggacttg accaaccaac gggccgaact gcacgcggcc
     3361 ggctgcacca agctgttttc cgagaagatc accggcacca ggcgcgaccg cccggagctg
     3421 gccaggatgc ttgaccacct acgccctggc gacgttgtga cagtgaccag gctagaccgc
     3481 ctggcccgca gcacccgcga cctactggac attgccgagc gcatccagga ggccggcgcg
     3541 ggcctgcgta gcctggcaga gccgtgggcc gacaccacca cgccggccgg ccgcatggtg
     3601 ttgaccgtgt tcgccggcat tgccgagttc gagcgttccc taatcatcga ccgcacccgg
     3661 agcgggcgcg aggccgccaa ggcccgaggc gtgaagtttg gcccccgccc taccctcacc
     3721 ccggcacaga tcgcgcacgc ccgcgagctg atcgaccagg aaggccgcac cgtgaaagag
     3781 gcggctgcac tgcttggcgt gcatcgctcg accctgtacc gcgcacttga gcgcagcgag
     3841 gaagtgacgc ccaccgaggc caggcggcgc ggtgccttcc gtgaggacgc attgaccgag
     3901 gccgacgccc tggcggccgc cgagaatgaa cgccaagagg aacaagcatg aaaccgcacc
     3961 aggacggcca ggacgaaccg tttttcatta ccgaagagat cgaggcggag atgatcgcgg
     4021 ccgggtacgt gttcgagccg cccgcgcacg tctcaaccgt gcggctgcat gaaatcctgg
     4081 ccggtttgtc tgatgccaag ctggcggcct ggccggccag cttggccgct gaagaaaccg
     4141 agcgccgccg tctaaaaagg tgatgtgtat ttgagtaaaa cagcttgcgt catgcggtcg
     4201 ctgcgtatat gatgcgatga gtaaataaac aaatacgcaa ggggaacgca tgaaggttat
     4261 cgctgtactt aaccagaaag gcgggtcagg caagacgacc atcgcaaccc atctagcccg
     4321 cgccctgcaa ctcgccgggg ccgatgttct gttagtcgat tccgatcccc agggcagtgc
     4381 ccgcgattgg gcggccgtgc gggaagatca accgctaacc gttgtcggca tcgaccgccc
     4441 gacgattgac cgcgacgtga aggccatcgg ccggcgcgac ttcgtagtga tcgacggagc
     4501 gccccaggcg gcggacttgg ctgtgtccgc gatcaaggca gccgacttcg tgctgattcc
     4561 ggtgcagcca agcccttacg acatatgggc caccgccgac ctggtggagc tggttaagca
     4621 gcgcattgag gtcacggatg gaaggctaca agcggccttt gtcgtgtcgc gggcgatcaa
     4681 aggcacgcgc atcggcggtg aggttgccga ggcgctggcc gggtacgagc tgcccattct
     4741 tgagtcccgt atcacgcagc gcgtgagcta cccaggcact gccgccgccg gcacaaccgt
     4801 tcttgaatca gaacccgagg gcgacgctgc ccgcgaggtc caggcgctgg ccgctgaaat
     4861 taaatcaaaa ctcatttgag ttaatgaggt aaagagaaaa tgagcaaaag cacaaacacg
     4921 ctaagtgccg gccgtccgag cgcacgcagc agcaaggctg caacgttggc cagcctggca
     4981 gacacgccag ccatgaagcg ggtcaacttt cagttgccgg cggaggatca caccaagctg
     5041 aagatgtacg cggtacgcca aggcaagacc attaccgagc tgctatctga atacatcgcg
     5101 cagctaccag agtaaatgag caaatgaata aatgagtaga tgaattttag cggctaaagg
     5161 aggcggcatg gaaaatcaag aacaaccagg caccgacgcc gtggaatgcc ccatgtgtgg
     5221 aggaacgggc ggttggccag gcgtaagcgg ctgggttgcc tgccggccct gcaatggcac
     5281 tggaaccccc aagcccgagg aatcggcgtg agcggtcgca aaccatccgg cccggtacaa
     5341 atcggcgcgg cgctgggtga tgacctggtg gagaagttga aggccgcgca ggccgcccag
     5401 cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga
     5461 atccgcaaag aatcccggca accgccggca gccggtgcgc cgtcgattag gaagccgccc
     5521 aagggcgacg agcaaccaga ttttttcgtt ccgatgctct atgacgtggg cacccgcgat
     5581 agtcgcagca tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg acgagctggc
     5641 gaggtgatcc gctacgagct tccagacggg cacgtagagg tttccgcagg gccggccggc
     5701 atggccagtg tgtgggatta cgacctggta ctgatggcgg tttcccatct aaccgaatcc
     5761 atgaaccgat accgggaagg gaagggagac aagcccggcc gcgtgttccg tccacacgtt
     5821 gcggacgtac tcaagttctg ccggcgagcc gatggcggaa agcagaaaga cgacctggta
     5881 gaaacctgca ttcggttaaa caccacgcac gttgccatgc agcgtacgaa gaaggccaag
     5941 aacggccgcc tggtgacggt atccgagggt gaagccttga ttagccgcta caagatcgta
     6001 aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc tagctgattg gatgtaccgc
     6061 gagatcacag aaggcaagaa cccggacgtg ctgacggttc accccgatta ctttttgatc
     6121 gatcccggca tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa
     6181 gccagatggt tgttcaagac gatctacgaa cgcagtggca gcgccggaga gttcaagaag
     6241 ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta cgatttgaag
     6301 gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct accgcaacct gatcgagggc
     6361 gaagcatccg ccggttccta atgtacggag cagatgctag ggcaaattgc cctagcaggg
     6421 gaaaaaggtc gaaaaggtct ctttcctgtg gatagcacgt acattgggaa cccaaagccg
     6481 tacattggga accggaaccc gtacattggg aacccaaagc cgtacattgg gaaccggtca
     6541 cacatgtaag tgactgatat aaaagagaaa aaaggcgatt tttccgccta aaactcttta
     6601 aaacttatta aaactcttaa aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca
     6661 gccgaagagc tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg ccccgccgct
     6721 tcgcgtcggc ctatcgcggc cgctggccgc tcaaaaatgg ctggcctacg gccaggcaat
     6781 ctaccagggc gcggacaagc cgcgccgtcg ccactcgacc gccggcgccc acatcaaggc
     6841 accctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
     6901 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
     6961 agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt
     7021 gtatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg
     7081 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc
     7141 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     7201 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     7261 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     7321 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     7381 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     7441 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     7501 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     7561 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     7621 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     7681 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     7741 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     7801 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     7861 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     7921 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgcattct
     7981 aggtactaaa acaattcatc cagtaaaata taatatttta ttttctccca atcaggcttg
     8041 atccccagta agtcaaaaaa tagctcgaca tactgttctt ccccgatatc ctccctgatc
     8101 gaccggacgc agaaggcaat gtcataccac ttgtccgccc tgccgcttct cccaagatca
     8161 ataaagccac ttactttgcc atctttcaca aagatgttgc tgtctcccag gtcgccgtgg
     8221 gaaaagacaa gttcctcttc gggcttttcc gtctttaaaa aatcatacag ctcgcgcgga
     8281 tctttaaatg gagtgtcttc ttcccagttt tcgcaatcca catcggccag atcgttattc
     8341 agtaagtaat ccaattcggc taagcggctg tctaagctat tcgtataggg acaatccgat
     8401 atgtcgatgg agtgaaagag cctgatgcac tccgcataca gctcgataat cttttcaggg
     8461 ctttgttcat cttcatactc ttccgagcaa aggacgccat cggcctcact catgagcaga
     8521 ttgctccagc catcatgccg ttcaaagtgc aggacctttg gaacaggcag ctttccttcc
     8581 agccatagca tcatgtcctt ttcccgttcc acatcatagg tggtcccttt ataccggctg
     8641 tccgtcattt ttaaatatag gttttcattt tctcccacca gcttatatac cttagcagga
     8701 gacattcctt ccgtatcttt tacgcagcgg tatttttcga tcagtttttt caattccggt
     8761 gatattctca ttttagccat ttattatttc cttcctcttt tctacagtat ttaaagatac
     8821 cccaagaagc taattataac aagacgaact ccaattcact gttccttgca ttctaaaacc
     8881 ttaaatacca gaaaacagct ttttcaaagt tgttttcaaa gttggcgtat aacatagtat
     8941 cgacggagcc gattttgaaa ccgcggtgat cacaggcagc aacgctctgt catcgttaca
     9001 atcaacatgc taccctccgc gagatcatcc gtgtttcaaa cccggcagct tagttgccgt
     9061 tcttccgaat agcatcggta acatgagcaa agtctgccgc cttacaacgg ctctcccgct
     9121 gacgccgtcc cggactgatg ggctgcctgt atcgagtggt gattttgtgc cgagctgccg
     9181 gtcggggagc tgttggctgg ctggtggcag gatatattgt ggtgtaaaca aattgacgct
     9241 tagacaactt aataacacat tgcggacgtt tttaatgtac tgaattaacg ccgaattaat
     9301 tcgggggatc tggattttag tactggattt tggttttagg aattagaaat tttattgata
     9361 gaagtatttt acaaatacaa atacatacta agggtttctt atatgctcaa cacatgagcg
     9421 aaaccctata ggaaccctaa ttcccttatc tgggaactac tcacacatta ttatggagaa
     9481 actcgagctt gtcgatcgac agatcccggt cggcatctac tctatttctt tgccctcgga
     9541 cgagtgctgg ggcgtcggtt tccactatcg gcgagtactt ctacacagcc atcggtccag
     9601 acggccgcgc ttctgcgggc gatttgtgta cgcccgacag tcccggctcc ggatcggacg
     9661 attgcgtcgc atcgaccctg cgcccaagct gcatcatcga aattgccgtc aaccaagctc
     9721 tgatagagtt ggtcaagacc aatgcggagc atatacgccc ggagtcgtgg cgatcctgca
     9781 agctccggat gcctccgctc gaagtagcgc gtctgctgct ccatacaagc caaccacggc
     9841 ctccagaaga agatgttggc gacctcgtat tgggaatccc cgaacatcgc ctcgctccag
     9901 tcaatgaccg ctgttatgcg gccattgtcc gtcaggacat tgttggagcc gaaatccgcg
     9961 tgcacgaggt gccggacttc ggggcagtcc tcggcccaaa gcatcagctc atcgagagcc
    10021 tgcgcgacgg acgcactgac ggtgtcgtcc atcacagttt gccagtgata cacatgggga
    10081 tcagcaatcg cgcatatgaa atcacgccat gtagtgtatt gaccgattcc ttgcggtccg
    10141 aatgggccga acccgctcgt ctggctaaga tcggccgcag cgatcgcatc catagcctcc
    10201 gcgaccggtt gtagaacagc gggcagttcg gtttcaggca ggtcttgcaa cgtgacaccc
    10261 tgtgaacggc gggagatgca ataggtcagg ctctcgctaa actccccaat gtcaagcact
    10321 tccggaatcg ggagcgcggc cgatgcaaag tgccgataaa cataacgatc tttgtagaaa
    10381 ccatcggcgc agctatttac ccgcaggaca tatccacgcc ctcctacatc gaagctgaaa
    10441 gcacgagatt cttcgccctc cgagagctgc atcaggtcgg agacgctgtc gaacttttcg
    10501 atcagaaact tctcgacaga cgtcgcggtg agttcaggct ttttcatatc tcattgcccc
    10561 ccggatctgc gaaagctcga gagagataga tttgtagaga gagactggtg atttcagcgt
    10621 gtcctctcca aatgaaatga acttccttat atagaggaag ggtcttgcga aggatagtgg
    10681 gattgtgcgt catcccttac gtcagtggag atatcacatc aatccacttg ctttgaagac
    10741 gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc atctttggga
    10801 ccactgtcgg cagaggcatc ttgaacgata gcctttcctt tatcgcaatg atggcatttg
    10861 taggtgccac cttccttttc tactgtcctt ttgatgaagt gacagatagc tgggcaatgg
    10921 aatccgagga ggtttcccga tattaccctt tgttgaaaag tctcaatagc cctttggtct
    10981 tctgagactg tatctttgat attcttggag tagacgagag tgtcgtgctc caccatgttg
    11041 gcaagctgct ctagccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
    11101 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
    11161 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
    11221 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac atgattacga
    11281 attcccgatc tagtaacata gatgacaccg cgcgcgataa tttatcctag tttgcgcgct
    11341 atattttgtt ttctatcgcg tattaaatgt ataattgcgg gactctaatc ataaaaaccc
    11401 atctcataaa taacgtcatg cattacatgt taattattac atgcttaacg taattcaaca
    11461 gaaattatat gataatcatc gcaagaccgg caacaggatt caatcttaag aaactttatt
    11521 gccaaatgtt tgaacgatcg gggaaattcg agctct
//
