LOCUS       sequence-455590-       11587 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mapping of cytosol-facing chloroplast outer membrane proximity 
            proteome by proximity-dependent biotinylation in living Arabidopsis 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11587)
  AUTHORS   Bao X, Jia H, Zhang X, Tian S, Zhao Y, Li X, Lin P, Ma C, Wang P, 
            Song CP, Zhu X
  TITLE     Mapping of cytosol-facing organelle outer membrane proximity 
            proteome by proximity-dependent biotinylation in living Arabidopsis 
            cells.
  JOURNAL   Plant J. 2024 Apr;118(1):7-23. doi: 10.1111/tpj.16641. Epub 2024 Jan
            23.
  PUBMED    38261530
REFERENCE   2  (bases 1 to 11587)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.16641";
            journalName: "Plant J"; date: "2024-04"; volume: "118"; issue: "1"; 
            pages: "7-23"
COMMENT     Sequence Label: CP4
FEATURES             Location/Qualifiers
     source          1..11587
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(32..97)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(122..148)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(161..187)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(200..226)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(242..1012)
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             complement(join(1016..1726,1727..1729,1730..1732))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(1016..1726,1727..1729,1730..1732))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1058..1079)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     1407..1426
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     promoter        complement(1852..2232)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     primer_bind     complement(2766..2783)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2766..2782)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2775..2797)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    2985..3009
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4310..4939
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5368..6441
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      6507..6701
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(6937..6959)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7045..7185
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7100..7119
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7200..7217)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7371..7959)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7451..7470)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8046..8840)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    9265..9289
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    9367..9541
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(9582..10607)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(10680..11025)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(10717..10737)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    11216..11237
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11252..11257,11258..11275,11276..11282)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11290..11306
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11295..11317
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11314..11330
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11314..11330
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      complement(join(11347..11587,1..12))
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
ORIGIN
        1 gtttgaacga tcggggaaat tcgagctctt acttatcgtc atcgtccttg taatcgatgt
       61 cgtgatcctt atagtctcca tcatggtctt tgtagtccat ggtaccggat ccactagtct
      121 aagcataatc aggcacatca taagggtatc cggcgctgcc agcgtagtca ggcacatcat
      181 aggggtagga tcctgtgccg gcgtaatcag gcacgtcata gggatacatg ccgctccctc
      241 ccttttcggc agaccgcaga ctgatttctc cgcccatcca gggtttgata actccgtcct
      301 gttccagcag cagggctccc tgtttatcaa tcccccggct aatcccgaaa atctctttgt
      361 ccccaatgat cagcttcacg ggtctattga tgaagttatc cagcttctcc caccgtgaca
      421 gatatggagc caggccttcc tgctcgaaca gttccagggc tgcccgcagc tctcggatca
      481 gcatagcggc cagagtattc ctgtccaggt taatccctgc ttcctgcagt gtgatccagc
      541 cctgattgac cacgctttcc tccacgcgcc tcatagccac gttaatcccg gcgccaatga
      601 cgatctgtgc agcatcgcct gttattccgg ccagctccac caggatgcct gccagctttc
      661 tatcctgcag atacaggtca ttgggccatt tgactcgcac cttgtctgct cccagctttc
      721 gcagcgcttc tgccatgaca attccgatga ccgggcccag gccgattgct gctggtcccc
      781 gcttcaggcg ccaaaacata ctcaggtaca ggttagctcc aaaaggagag aaccatttcc
      841 tccctctgct tcctctccct gcctgctgat attctgcaat gcaagcatcc ccactcttca
      901 gctcgccgat tcgatccagc aggtactgat tggtggagtc gaccacaggc aggactgcca
      961 cgctcccgcc gtccagctgt cccagaatct gtttagcgtt cagcagcggg atcatcttgt
     1021 acagctcgtc catgccgaga gtgatcccgg cggcggtcac gaactccagc aggaccatgt
     1081 gatcgcgctt ctcgttgggg tctttgctca gggcggactg ggtgctcagg tagtggttgt
     1141 cgggcagcag cacggggccg tcgccgatgg gggtgttctg ctggtagtgg tcggcgagct
     1201 gcacgctgcc gtcctcgatg ttgtggcgga tcttgaagtt caccttgatg ccgttcttct
     1261 gcttgtcggc catgatatag acgttgtggc tgttgtagtt gtactccagc ttgtgcccca
     1321 ggatgttgcc gtcctccttg aagtcgatgc ccttcagctc gatgcggttc accagggtgt
     1381 cgccctcgaa cttcacctcg gcgcgggtct tgtagttgcc gtcgtccttg aagaagatgg
     1441 tgcgctcctg gacgtagcct tcgggcatgg cggacttgaa gaagtcgtgc tgcttcatgt
     1501 ggtcggggta gcggctgaag cactgcacgc cgtaggtcag ggtggtcacg agggtgggcc
     1561 agggcacggg cagcttgccg gtggtgcaga tgaacttcag ggtcagcttg ccgtaggtgg
     1621 catcgccctc gccctcgccg gacacgctga acttgtggcc gtttacgtcg ccgtccagct
     1681 cgaccaggat gggcaccacc ccggtgaaca gctcctcgcc cttgctcacc atactagtat
     1741 cttcacggac ggtcttcttc agctttttgg taagcataag aatcccagcg agacctaaac
     1801 ctagaagaac ccagagattc gcagcttgag acgccataag ctttctagag tcgatttggt
     1861 gtatcgagat tggttatgaa attcagatgc tagtgtaatg tattggtaat ttgggaagat
     1921 ataataggaa gcaaggctat ttatccattt ctgaaaaggc gaaatggcgt caccgcgagc
     1981 gtcacgcgca ttccgttctt gctgtaaagc gttgtttggt acacttttga ctagcgaggc
     2041 ttggcgtgtc agcgtatcta ttcaaaagtc gttaatggct gcggatcaag aaaaagttgg
     2101 aatagaaaca gaatacccgc gaaattcagg cccggttgcc atgtcctaca cgccgaaata
     2161 aacgaccaaa ttagtagaaa aataaaaact gactcggata cttacgtcac gtcttgcgca
     2221 ctgatttgaa aaatctccct cgatcgagaa agagatcaat gttgagctgc ttcaaaagca
     2281 atgggattga ccagctcgcg gatcctacag gccaaattcg ctcttagccg tacaatatta
     2341 ctcaccggtg cgatgccccc catcgtaggt gaaggtggaa attaatgatc catcttgaga
     2401 ccacaggccc acaacagcta ccagtttcct caagggtcca ccaaaaacgt aagcgcttac
     2461 gtacatggtc gataagaaaa ggcaatttgt agatgttaac atccaacgtc gctttcaggg
     2521 atcctacagg ccaaattcgc tcttagccgt acaatattac tcaccggtgc gatgcccccc
     2581 atcgtaggtg aaggtggaaa ttaatgatcc atcttgagac cacaggccca caacagctac
     2641 cagtttcctc aagggtccac caaaaacgta agcgcttacg tacatggtcg ataagaaaag
     2701 gcaatttgta gatgttaaca tccaacgtcg ctttcaggga tccgcgagct tatcgatacc
     2761 gtggcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     2821 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     2881 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gctagagcag cttgagcttg
     2941 gatcagattg tcgtttcccg ccttcagttt aaactatcag tgtttgacag gatatattgg
     3001 cgggtaaacc taagagaaaa gagcgtttat tagaataatc ggatatttaa aagggcgtga
     3061 aaaggtttat ccgttcgtcc atttgtatgt gcatgccaac cacagggttc ccctcgggat
     3121 caaagtactt tgatccaacc cctccgctgc tatagtgcag tcggcttctg acgttcagtg
     3181 cagccgtctt ctgaaaacga catgtcgcac aagtcctaag ttacgcgaca ggctgccgcc
     3241 ctgccctttt cctggcgttt tcttgtcgcg tgttttagtc gcataaagta gaatacttgc
     3301 gactagaacc ggagacatta cgccatgaac aagagcgccg ccgctggcct gctgggctat
     3361 gcccgcgtca gcaccgacga ccaggacttg accaaccaac gggccgaact gcacgcggcc
     3421 ggctgcacca agctgttttc cgagaagatc accggcacca ggcgcgaccg cccggagctg
     3481 gccaggatgc ttgaccacct acgccctggc gacgttgtga cagtgaccag gctagaccgc
     3541 ctggcccgca gcacccgcga cctactggac attgccgagc gcatccagga ggccggcgcg
     3601 ggcctgcgta gcctggcaga gccgtgggcc gacaccacca cgccggccgg ccgcatggtg
     3661 ttgaccgtgt tcgccggcat tgccgagttc gagcgttccc taatcatcga ccgcacccgg
     3721 agcgggcgcg aggccgccaa ggcccgaggc gtgaagtttg gcccccgccc taccctcacc
     3781 ccggcacaga tcgcgcacgc ccgcgagctg atcgaccagg aaggccgcac cgtgaaagag
     3841 gcggctgcac tgcttggcgt gcatcgctcg accctgtacc gcgcacttga gcgcagcgag
     3901 gaagtgacgc ccaccgaggc caggcggcgc ggtgccttcc gtgaggacgc attgaccgag
     3961 gccgacgccc tggcggccgc cgagaatgaa cgccaagagg aacaagcatg aaaccgcacc
     4021 aggacggcca ggacgaaccg tttttcatta ccgaagagat cgaggcggag atgatcgcgg
     4081 ccgggtacgt gttcgagccg cccgcgcacg tctcaaccgt gcggctgcat gaaatcctgg
     4141 ccggtttgtc tgatgccaag ctggcggcct ggccggccag cttggccgct gaagaaaccg
     4201 agcgccgccg tctaaaaagg tgatgtgtat ttgagtaaaa cagcttgcgt catgcggtcg
     4261 ctgcgtatat gatgcgatga gtaaataaac aaatacgcaa ggggaacgca tgaaggttat
     4321 cgctgtactt aaccagaaag gcgggtcagg caagacgacc atcgcaaccc atctagcccg
     4381 cgccctgcaa ctcgccgggg ccgatgttct gttagtcgat tccgatcccc agggcagtgc
     4441 ccgcgattgg gcggccgtgc gggaagatca accgctaacc gttgtcggca tcgaccgccc
     4501 gacgattgac cgcgacgtga aggccatcgg ccggcgcgac ttcgtagtga tcgacggagc
     4561 gccccaggcg gcggacttgg ctgtgtccgc gatcaaggca gccgacttcg tgctgattcc
     4621 ggtgcagcca agcccttacg acatatgggc caccgccgac ctggtggagc tggttaagca
     4681 gcgcattgag gtcacggatg gaaggctaca agcggccttt gtcgtgtcgc gggcgatcaa
     4741 aggcacgcgc atcggcggtg aggttgccga ggcgctggcc gggtacgagc tgcccattct
     4801 tgagtcccgt atcacgcagc gcgtgagcta cccaggcact gccgccgccg gcacaaccgt
     4861 tcttgaatca gaacccgagg gcgacgctgc ccgcgaggtc caggcgctgg ccgctgaaat
     4921 taaatcaaaa ctcatttgag ttaatgaggt aaagagaaaa tgagcaaaag cacaaacacg
     4981 ctaagtgccg gccgtccgag cgcacgcagc agcaaggctg caacgttggc cagcctggca
     5041 gacacgccag ccatgaagcg ggtcaacttt cagttgccgg cggaggatca caccaagctg
     5101 aagatgtacg cggtacgcca aggcaagacc attaccgagc tgctatctga atacatcgcg
     5161 cagctaccag agtaaatgag caaatgaata aatgagtaga tgaattttag cggctaaagg
     5221 aggcggcatg gaaaatcaag aacaaccagg caccgacgcc gtggaatgcc ccatgtgtgg
     5281 aggaacgggc ggttggccag gcgtaagcgg ctgggttgcc tgccggccct gcaatggcac
     5341 tggaaccccc aagcccgagg aatcggcgtg agcggtcgca aaccatccgg cccggtacaa
     5401 atcggcgcgg cgctgggtga tgacctggtg gagaagttga aggccgcgca ggccgcccag
     5461 cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga
     5521 atccgcaaag aatcccggca accgccggca gccggtgcgc cgtcgattag gaagccgccc
     5581 aagggcgacg agcaaccaga ttttttcgtt ccgatgctct atgacgtggg cacccgcgat
     5641 agtcgcagca tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg acgagctggc
     5701 gaggtgatcc gctacgagct tccagacggg cacgtagagg tttccgcagg gccggccggc
     5761 atggccagtg tgtgggatta cgacctggta ctgatggcgg tttcccatct aaccgaatcc
     5821 atgaaccgat accgggaagg gaagggagac aagcccggcc gcgtgttccg tccacacgtt
     5881 gcggacgtac tcaagttctg ccggcgagcc gatggcggaa agcagaaaga cgacctggta
     5941 gaaacctgca ttcggttaaa caccacgcac gttgccatgc agcgtacgaa gaaggccaag
     6001 aacggccgcc tggtgacggt atccgagggt gaagccttga ttagccgcta caagatcgta
     6061 aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc tagctgattg gatgtaccgc
     6121 gagatcacag aaggcaagaa cccggacgtg ctgacggttc accccgatta ctttttgatc
     6181 gatcccggca tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa
     6241 gccagatggt tgttcaagac gatctacgaa cgcagtggca gcgccggaga gttcaagaag
     6301 ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta cgatttgaag
     6361 gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct accgcaacct gatcgagggc
     6421 gaagcatccg ccggttccta atgtacggag cagatgctag ggcaaattgc cctagcaggg
     6481 gaaaaaggtc gaaaaggtct ctttcctgtg gatagcacgt acattgggaa cccaaagccg
     6541 tacattggga accggaaccc gtacattggg aacccaaagc cgtacattgg gaaccggtca
     6601 cacatgtaag tgactgatat aaaagagaaa aaaggcgatt tttccgccta aaactcttta
     6661 aaacttatta aaactcttaa aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca
     6721 gccgaagagc tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg ccccgccgct
     6781 tcgcgtcggc ctatcgcggc cgctggccgc tcaaaaatgg ctggcctacg gccaggcaat
     6841 ctaccagggc gcggacaagc cgcgccgtcg ccactcgacc gccggcgccc acatcaaggc
     6901 accctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
     6961 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
     7021 agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt
     7081 gtatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg
     7141 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc
     7201 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     7261 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     7321 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     7381 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     7441 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     7501 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     7561 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     7621 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     7681 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     7741 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     7801 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     7861 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     7921 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     7981 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgcattct
     8041 aggtactaaa acaattcatc cagtaaaata taatatttta ttttctccca atcaggcttg
     8101 atccccagta agtcaaaaaa tagctcgaca tactgttctt ccccgatatc ctccctgatc
     8161 gaccggacgc agaaggcaat gtcataccac ttgtccgccc tgccgcttct cccaagatca
     8221 ataaagccac ttactttgcc atctttcaca aagatgttgc tgtctcccag gtcgccgtgg
     8281 gaaaagacaa gttcctcttc gggcttttcc gtctttaaaa aatcatacag ctcgcgcgga
     8341 tctttaaatg gagtgtcttc ttcccagttt tcgcaatcca catcggccag atcgttattc
     8401 agtaagtaat ccaattcggc taagcggctg tctaagctat tcgtataggg acaatccgat
     8461 atgtcgatgg agtgaaagag cctgatgcac tccgcataca gctcgataat cttttcaggg
     8521 ctttgttcat cttcatactc ttccgagcaa aggacgccat cggcctcact catgagcaga
     8581 ttgctccagc catcatgccg ttcaaagtgc aggacctttg gaacaggcag ctttccttcc
     8641 agccatagca tcatgtcctt ttcccgttcc acatcatagg tggtcccttt ataccggctg
     8701 tccgtcattt ttaaatatag gttttcattt tctcccacca gcttatatac cttagcagga
     8761 gacattcctt ccgtatcttt tacgcagcgg tatttttcga tcagtttttt caattccggt
     8821 gatattctca ttttagccat ttattatttc cttcctcttt tctacagtat ttaaagatac
     8881 cccaagaagc taattataac aagacgaact ccaattcact gttccttgca ttctaaaacc
     8941 ttaaatacca gaaaacagct ttttcaaagt tgttttcaaa gttggcgtat aacatagtat
     9001 cgacggagcc gattttgaaa ccgcggtgat cacaggcagc aacgctctgt catcgttaca
     9061 atcaacatgc taccctccgc gagatcatcc gtgtttcaaa cccggcagct tagttgccgt
     9121 tcttccgaat agcatcggta acatgagcaa agtctgccgc cttacaacgg ctctcccgct
     9181 gacgccgtcc cggactgatg ggctgcctgt atcgagtggt gattttgtgc cgagctgccg
     9241 gtcggggagc tgttggctgg ctggtggcag gatatattgt ggtgtaaaca aattgacgct
     9301 tagacaactt aataacacat tgcggacgtt tttaatgtac tgaattaacg ccgaattaat
     9361 tcgggggatc tggattttag tactggattt tggttttagg aattagaaat tttattgata
     9421 gaagtatttt acaaatacaa atacatacta agggtttctt atatgctcaa cacatgagcg
     9481 aaaccctata ggaaccctaa ttcccttatc tgggaactac tcacacatta ttatggagaa
     9541 actcgagctt gtcgatcgac agatcccggt cggcatctac tctatttctt tgccctcgga
     9601 cgagtgctgg ggcgtcggtt tccactatcg gcgagtactt ctacacagcc atcggtccag
     9661 acggccgcgc ttctgcgggc gatttgtgta cgcccgacag tcccggctcc ggatcggacg
     9721 attgcgtcgc atcgaccctg cgcccaagct gcatcatcga aattgccgtc aaccaagctc
     9781 tgatagagtt ggtcaagacc aatgcggagc atatacgccc ggagtcgtgg cgatcctgca
     9841 agctccggat gcctccgctc gaagtagcgc gtctgctgct ccatacaagc caaccacggc
     9901 ctccagaaga agatgttggc gacctcgtat tgggaatccc cgaacatcgc ctcgctccag
     9961 tcaatgaccg ctgttatgcg gccattgtcc gtcaggacat tgttggagcc gaaatccgcg
    10021 tgcacgaggt gccggacttc ggggcagtcc tcggcccaaa gcatcagctc atcgagagcc
    10081 tgcgcgacgg acgcactgac ggtgtcgtcc atcacagttt gccagtgata cacatgggga
    10141 tcagcaatcg cgcatatgaa atcacgccat gtagtgtatt gaccgattcc ttgcggtccg
    10201 aatgggccga acccgctcgt ctggctaaga tcggccgcag cgatcgcatc catagcctcc
    10261 gcgaccggtt gtagaacagc gggcagttcg gtttcaggca ggtcttgcaa cgtgacaccc
    10321 tgtgaacggc gggagatgca ataggtcagg ctctcgctaa actccccaat gtcaagcact
    10381 tccggaatcg ggagcgcggc cgatgcaaag tgccgataaa cataacgatc tttgtagaaa
    10441 ccatcggcgc agctatttac ccgcaggaca tatccacgcc ctcctacatc gaagctgaaa
    10501 gcacgagatt cttcgccctc cgagagctgc atcaggtcgg agacgctgtc gaacttttcg
    10561 atcagaaact tctcgacaga cgtcgcggtg agttcaggct ttttcatatc tcattgcccc
    10621 ccggatctgc gaaagctcga gagagataga tttgtagaga gagactggtg atttcagcgt
    10681 gtcctctcca aatgaaatga acttccttat atagaggaag ggtcttgcga aggatagtgg
    10741 gattgtgcgt catcccttac gtcagtggag atatcacatc aatccacttg ctttgaagac
    10801 gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc atctttggga
    10861 ccactgtcgg cagaggcatc ttgaacgata gcctttcctt tatcgcaatg atggcatttg
    10921 taggtgccac cttccttttc tactgtcctt ttgatgaagt gacagatagc tgggcaatgg
    10981 aatccgagga ggtttcccga tattaccctt tgttgaaaag tctcaatagc cctttggtct
    11041 tctgagactg tatctttgat attcttggag tagacgagag tgtcgtgctc caccatgttg
    11101 gcaagctgct ctagccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
    11161 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
    11221 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
    11281 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac atgattacga
    11341 attcccgatc tagtaacata gatgacaccg cgcgcgataa tttatcctag tttgcgcgct
    11401 atattttgtt ttctatcgcg tattaaatgt ataattgcgg gactctaatc ataaaaaccc
    11461 atctcataaa taacgtcatg cattacatgt taattattac atgcttaacg taattcaaca
    11521 gaaattatat gataatcatc gcaagaccgg caacaggatt caatcttaag aaactttatt
    11581 gccaaat
//
