LOCUS       sequence-455591-       11707 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mapping of cytosol-facing chloroplast outer membrane proximity 
            proteome by proximity-dependent biotinylation in living Arabidopsis 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11707)
  AUTHORS   Bao X, Jia H, Zhang X, Tian S, Zhao Y, Li X, Lin P, Ma C, Wang P, 
            Song CP, Zhu X
  TITLE     Mapping of cytosol-facing organelle outer membrane proximity 
            proteome by proximity-dependent biotinylation in living Arabidopsis 
            cells.
  JOURNAL   Plant J. 2024 Apr;118(1):7-23. doi: 10.1111/tpj.16641. Epub 2024 Jan
            23.
  PUBMED    38261530
REFERENCE   2  (bases 1 to 11707)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.16641";
            journalName: "Plant J"; date: "2024-04"; volume: "118"; issue: "1"; 
            pages: "7-23"
COMMENT     Sequence Label: CP1
FEATURES             Location/Qualifiers
     source          1..11707
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(33..59)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(join(81..791,792..794,795..797))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDDLYK"
     primer_bind     complement(123..144)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     472..491
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             complement(816..1775)
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID 
                     engineered for improved catalytic efficiency (Branon et 
                     al., 2018)"
                     /label=TurboID
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     promoter        complement(1895..2275)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     primer_bind     complement(2809..2826)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2809..2825)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2818..2840)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    3028..3052
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4353..4982
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5411..6484
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      6550..6744
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(6980..7002)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7088..7228
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7143..7162
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7243..7260)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7414..8002)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7494..7513)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8089..8883)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    9308..9332
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    9410..9584
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(9625..10650)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(10723..11068)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(10760..10780)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    11259..11280
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11295..11300,11301..11318,11319..11325)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11333..11349
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11338..11360
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11357..11373
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11357..11373
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      complement(11390..11642)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(11662..11688)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(join(11701..11707,1..20))
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 tcaggcacat cataggggta ggatcctgtg ccggcgtaat caggcacgtc atagggatac
       61 atgccgctcc ctccgagctc cttgtacagg tcgtccatgc cgagagtgat cccggcggcg
      121 gtcacgaact ccagcaggac catgtgatcg cgcttctcgt tggggtcttt gctcagggcg
      181 gactgggtgc tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg
      241 ttctgctggt agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg
      301 aagttcacct tgatgccgtt cttctgcttg tcggccatga tatagacgtt gtggctgttg
      361 tagttgtact ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc
      421 agctcgatgc ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag
      481 ttgccgtcgt ccttgaagaa gatggtgcgc tcctggacgt agccttcggg catggcggac
      541 ttgaagaagt cgtgctgctt catgtggtcg gggtagcggc tgaagcactg cacgccgtag
      601 gtcagggtgg tcacgagggt gggccagggc acgggcagct tgccggtggt gcagatgaac
      661 ttcagggtca gcttgccgta ggtggcatcg ccctcgccct cgccggacac gctgaacttg
      721 tggccgttta cgtcgccgtc cagctcgacc aggatgggca ccaccccggt gaacagctcc
      781 tcgcccttgc tcaccatggt accggatcca ctagtctttt cggcagaccg cagactgatt
      841 tctccgccca tccagggttt gataactccg tcctgttcca gcagcagggc tccctgttta
      901 tcaatccccc ggctaatccc gaaaatctct ttgtccccaa tgatcagctt cacgggtcta
      961 ttgatgaagt tatccagctt ctcccaccgt ggcagatatg gagccaggcc ttcctgctcg
     1021 aacagttcca gggctgcccg cagctctcgg atcagcgtag cggccagagt attcctgtcc
     1081 aggttaatcc ctgcttcctg cagtgtgatc cagccctgat tgaccacgct ttcctccacg
     1141 cgcctcatag ccacgttaat cccggcgcca atgacgatct gtgcagcatc gcctgttatt
     1201 ccggccagct ccaccaggat gcctgccagc tttctatcct gcagatacag gtcattgggc
     1261 catttgactc gcaccttgtc tgctcccagc tttcgcagcg cttctgccat gacaattccg
     1321 atgaccgggc ccaggccgat tgctgctggt ccccgcttca ggcgccaaaa catactcagg
     1381 tacaggttag ctccaaaagg agagaaccat ttcctccctc tgcttcctct ccctgcctgc
     1441 tgatattctg caatgcaagc atccccactc ttcagctcgc cgattcgatc cagcaggtac
     1501 tgattggtgg agtcgaccac aggcaggact gccacgctcc cgccgtccag ctgtcccaga
     1561 atctgtttag cgttcagcag cgggataggc tcgggcagag agtagccctt tccgggcacg
     1621 gtgaacacgt ccacgcccca gtcgcgcaga gtctgaatgt gcttgttgat agcggccctg
     1681 gacatgccca gggtttctcc cagctgttcg ccactatgga actcgccatt agccaggaga
     1741 gcgatcagct tcagaggcac agtattgtct ttcatactag tatcttcacg gacggtcttc
     1801 ttcagctttt tggtaagcat aagaatccca gcgagaccta aacctagaag aacccagaga
     1861 ttcgcagctt gagacgccat aagctttcta gagtcgattt ggtgtatcga gattggttat
     1921 gaaattcaga tgctagtgta atgtattggt aatttgggaa gatataatag gaagcaaggc
     1981 tatttatcca tttctgaaaa ggcgaaatgg cgtcaccgcg agcgtcacgc gcattccgtt
     2041 cttgctgtaa agcgttgttt ggtacacttt tgactagcga ggcttggcgt gtcagcgtat
     2101 ctattcaaaa gtcgttaatg gctgcggatc aagaaaaagt tggaatagaa acagaatacc
     2161 cgcgaaattc aggcccggtt gccatgtcct acacgccgaa ataaacgacc aaattagtag
     2221 aaaaataaaa actgactcgg atacttacgt cacgtcttgc gcactgattt gaaaaatctc
     2281 cctcgatcga gaaagagatc aatgttgagc tgcttcaaaa gcaatgggat tgaccagctc
     2341 gcggatccta caggccaaat tcgctcttag ccgtacaata ttactcaccg gtgcgatgcc
     2401 ccccatcgta ggtgaaggtg gaaattaatg atccatcttg agaccacagg cccacaacag
     2461 ctaccagttt cctcaagggt ccaccaaaaa cgtaagcgct tacgtacatg gtcgataaga
     2521 aaaggcaatt tgtagatgtt aacatccaac gtcgctttca gggatcctac aggccaaatt
     2581 cgctcttagc cgtacaatat tactcaccgg tgcgatgccc cccatcgtag gtgaaggtgg
     2641 aaattaatga tccatcttga gaccacaggc ccacaacagc taccagtttc ctcaagggtc
     2701 caccaaaaac gtaagcgctt acgtacatgg tcgataagaa aaggcaattt gtagatgtta
     2761 acatccaacg tcgctttcag ggatccgcga gcttatcgat accgtggcac tggccgtcgt
     2821 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     2881 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     2941 gttgcgcagc ctgaatggcg aatgctagag cagcttgagc ttggatcaga ttgtcgtttc
     3001 ccgccttcag tttaaactat cagtgtttga caggatatat tggcgggtaa acctaagaga
     3061 aaagagcgtt tattagaata atcggatatt taaaagggcg tgaaaaggtt tatccgttcg
     3121 tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg gatcaaagta ctttgatcca
     3181 acccctccgc tgctatagtg cagtcggctt ctgacgttca gtgcagccgt cttctgaaaa
     3241 cgacatgtcg cacaagtcct aagttacgcg acaggctgcc gccctgccct tttcctggcg
     3301 ttttcttgtc gcgtgtttta gtcgcataaa gtagaatact tgcgactaga accggagaca
     3361 ttacgccatg aacaagagcg ccgccgctgg cctgctgggc tatgcccgcg tcagcaccga
     3421 cgaccaggac ttgaccaacc aacgggccga actgcacgcg gccggctgca ccaagctgtt
     3481 ttccgagaag atcaccggca ccaggcgcga ccgcccggag ctggccagga tgcttgacca
     3541 cctacgccct ggcgacgttg tgacagtgac caggctagac cgcctggccc gcagcacccg
     3601 cgacctactg gacattgccg agcgcatcca ggaggccggc gcgggcctgc gtagcctggc
     3661 agagccgtgg gccgacacca ccacgccggc cggccgcatg gtgttgaccg tgttcgccgg
     3721 cattgccgag ttcgagcgtt ccctaatcat cgaccgcacc cggagcgggc gcgaggccgc
     3781 caaggcccga ggcgtgaagt ttggcccccg ccctaccctc accccggcac agatcgcgca
     3841 cgcccgcgag ctgatcgacc aggaaggccg caccgtgaaa gaggcggctg cactgcttgg
     3901 cgtgcatcgc tcgaccctgt accgcgcact tgagcgcagc gaggaagtga cgcccaccga
     3961 ggccaggcgg cgcggtgcct tccgtgagga cgcattgacc gaggccgacg ccctggcggc
     4021 cgccgagaat gaacgccaag aggaacaagc atgaaaccgc accaggacgg ccaggacgaa
     4081 ccgtttttca ttaccgaaga gatcgaggcg gagatgatcg cggccgggta cgtgttcgag
     4141 ccgcccgcgc acgtctcaac cgtgcggctg catgaaatcc tggccggttt gtctgatgcc
     4201 aagctggcgg cctggccggc cagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa
     4261 aggtgatgtg tatttgagta aaacagcttg cgtcatgcgg tcgctgcgta tatgatgcga
     4321 tgagtaaata aacaaatacg caaggggaac gcatgaaggt tatcgctgta cttaaccaga
     4381 aaggcgggtc aggcaagacg accatcgcaa cccatctagc ccgcgccctg caactcgccg
     4441 gggccgatgt tctgttagtc gattccgatc cccagggcag tgcccgcgat tgggcggccg
     4501 tgcgggaaga tcaaccgcta accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg
     4561 tgaaggccat cggccggcgc gacttcgtag tgatcgacgg agcgccccag gcggcggact
     4621 tggctgtgtc cgcgatcaag gcagccgact tcgtgctgat tccggtgcag ccaagccctt
     4681 acgacatatg ggccaccgcc gacctggtgg agctggttaa gcagcgcatt gaggtcacgg
     4741 atggaaggct acaagcggcc tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg
     4801 gtgaggttgc cgaggcgctg gccgggtacg agctgcccat tcttgagtcc cgtatcacgc
     4861 agcgcgtgag ctacccaggc actgccgccg ccggcacaac cgttcttgaa tcagaacccg
     4921 agggcgacgc tgcccgcgag gtccaggcgc tggccgctga aattaaatca aaactcattt
     4981 gagttaatga ggtaaagaga aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc
     5041 gagcgcacgc agcagcaagg ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa
     5101 gcgggtcaac tttcagttgc cggcggagga tcacaccaag ctgaagatgt acgcggtacg
     5161 ccaaggcaag accattaccg agctgctatc tgaatacatc gcgcagctac cagagtaaat
     5221 gagcaaatga ataaatgagt agatgaattt tagcggctaa aggaggcggc atggaaaatc
     5281 aagaacaacc aggcaccgac gccgtggaat gccccatgtg tggaggaacg ggcggttggc
     5341 caggcgtaag cggctgggtt gcctgccggc cctgcaatgg cactggaacc cccaagcccg
     5401 aggaatcggc gtgagcggtc gcaaaccatc cggcccggta caaatcggcg cggcgctggg
     5461 tgatgacctg gtggagaagt tgaaggccgc gcaggccgcc cagcggcaac gcatcgaggc
     5521 agaagcacgc cccggtgaat cgtggcaagc ggccgctgat cgaatccgca aagaatcccg
     5581 gcaaccgccg gcagccggtg cgccgtcgat taggaagccg cccaagggcg acgagcaacc
     5641 agattttttc gttccgatgc tctatgacgt gggcacccgc gatagtcgca gcatcatgga
     5701 cgtggccgtt ttccgtctgt cgaagcgtga ccgacgagct ggcgaggtga tccgctacga
     5761 gcttccagac gggcacgtag aggtttccgc agggccggcc ggcatggcca gtgtgtggga
     5821 ttacgacctg gtactgatgg cggtttccca tctaaccgaa tccatgaacc gataccggga
     5881 agggaaggga gacaagcccg gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt
     5941 ctgccggcga gccgatggcg gaaagcagaa agacgacctg gtagaaacct gcattcggtt
     6001 aaacaccacg cacgttgcca tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac
     6061 ggtatccgag ggtgaagcct tgattagccg ctacaagatc gtaaagagcg aaaccgggcg
     6121 gccggagtac atcgagatcg agctagctga ttggatgtac cgcgagatca cagaaggcaa
     6181 gaacccggac gtgctgacgg ttcaccccga ttactttttg atcgatcccg gcatcggccg
     6241 ttttctctac cgcctggcac gccgcgccgc aggcaaggca gaagccagat ggttgttcaa
     6301 gacgatctac gaacgcagtg gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg
     6361 caagctgatc gggtcaaatg acctgccgga gtacgatttg aaggaggagg cggggcaggc
     6421 tggcccgatc ctagtcatgc gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc
     6481 ctaatgtacg gagcagatgc tagggcaaat tgccctagca ggggaaaaag gtcgaaaagg
     6541 tctctttcct gtggatagca cgtacattgg gaacccaaag ccgtacattg ggaaccggaa
     6601 cccgtacatt gggaacccaa agccgtacat tgggaaccgg tcacacatgt aagtgactga
     6661 tataaaagag aaaaaaggcg atttttccgc ctaaaactct ttaaaactta ttaaaactct
     6721 taaaacccgc ctggcctgtg cataactgtc tggccagcgc acagccgaag agctgcaaaa
     6781 agcgcctacc cttcggtcgc tgcgctccct acgccccgcc gcttcgcgtc ggcctatcgc
     6841 ggccgctggc cgctcaaaaa tggctggcct acggccaggc aatctaccag ggcgcggaca
     6901 agccgcgccg tcgccactcg accgccggcg cccacatcaa ggcaccctgc ctcgcgcgtt
     6961 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
     7021 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
     7081 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta
     7141 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
     7201 atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct
     7261 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     7321 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     7381 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     7441 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     7501 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     7561 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     7621 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     7681 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     7741 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     7801 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     7861 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     7921 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     7981 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     8041 gtggaacgaa aactcacgtt aagggatttt ggtcatgcat tctaggtact aaaacaattc
     8101 atccagtaaa atataatatt ttattttctc ccaatcaggc ttgatcccca gtaagtcaaa
     8161 aaatagctcg acatactgtt cttccccgat atcctccctg atcgaccgga cgcagaaggc
     8221 aatgtcatac cacttgtccg ccctgccgct tctcccaaga tcaataaagc cacttacttt
     8281 gccatctttc acaaagatgt tgctgtctcc caggtcgccg tgggaaaaga caagttcctc
     8341 ttcgggcttt tccgtcttta aaaaatcata cagctcgcgc ggatctttaa atggagtgtc
     8401 ttcttcccag ttttcgcaat ccacatcggc cagatcgtta ttcagtaagt aatccaattc
     8461 ggctaagcgg ctgtctaagc tattcgtata gggacaatcc gatatgtcga tggagtgaaa
     8521 gagcctgatg cactccgcat acagctcgat aatcttttca gggctttgtt catcttcata
     8581 ctcttccgag caaaggacgc catcggcctc actcatgagc agattgctcc agccatcatg
     8641 ccgttcaaag tgcaggacct ttggaacagg cagctttcct tccagccata gcatcatgtc
     8701 cttttcccgt tccacatcat aggtggtccc tttataccgg ctgtccgtca tttttaaata
     8761 taggttttca ttttctccca ccagcttata taccttagca ggagacattc cttccgtatc
     8821 ttttacgcag cggtattttt cgatcagttt tttcaattcc ggtgatattc tcattttagc
     8881 catttattat ttccttcctc ttttctacag tatttaaaga taccccaaga agctaattat
     8941 aacaagacga actccaattc actgttcctt gcattctaaa accttaaata ccagaaaaca
     9001 gctttttcaa agttgttttc aaagttggcg tataacatag tatcgacgga gccgattttg
     9061 aaaccgcggt gatcacaggc agcaacgctc tgtcatcgtt acaatcaaca tgctaccctc
     9121 cgcgagatca tccgtgtttc aaacccggca gcttagttgc cgttcttccg aatagcatcg
     9181 gtaacatgag caaagtctgc cgccttacaa cggctctccc gctgacgccg tcccggactg
     9241 atgggctgcc tgtatcgagt ggtgattttg tgccgagctg ccggtcgggg agctgttggc
     9301 tggctggtgg caggatatat tgtggtgtaa acaaattgac gcttagacaa cttaataaca
     9361 cattgcggac gtttttaatg tactgaatta acgccgaatt aattcggggg atctggattt
     9421 tagtactgga ttttggtttt aggaattaga aattttattg atagaagtat tttacaaata
     9481 caaatacata ctaagggttt cttatatgct caacacatga gcgaaaccct ataggaaccc
     9541 taattccctt atctgggaac tactcacaca ttattatgga gaaactcgag cttgtcgatc
     9601 gacagatccc ggtcggcatc tactctattt ctttgccctc ggacgagtgc tggggcgtcg
     9661 gtttccacta tcggcgagta cttctacaca gccatcggtc cagacggccg cgcttctgcg
     9721 ggcgatttgt gtacgcccga cagtcccggc tccggatcgg acgattgcgt cgcatcgacc
     9781 ctgcgcccaa gctgcatcat cgaaattgcc gtcaaccaag ctctgataga gttggtcaag
     9841 accaatgcgg agcatatacg cccggagtcg tggcgatcct gcaagctccg gatgcctccg
     9901 ctcgaagtag cgcgtctgct gctccataca agccaaccac ggcctccaga agaagatgtt
     9961 ggcgacctcg tattgggaat ccccgaacat cgcctcgctc cagtcaatga ccgctgttat
    10021 gcggccattg tccgtcagga cattgttgga gccgaaatcc gcgtgcacga ggtgccggac
    10081 ttcggggcag tcctcggccc aaagcatcag ctcatcgaga gcctgcgcga cggacgcact
    10141 gacggtgtcg tccatcacag tttgccagtg atacacatgg ggatcagcaa tcgcgcatat
    10201 gaaatcacgc catgtagtgt attgaccgat tccttgcggt ccgaatgggc cgaacccgct
    10261 cgtctggcta agatcggccg cagcgatcgc atccatagcc tccgcgaccg gttgtagaac
    10321 agcgggcagt tcggtttcag gcaggtcttg caacgtgaca ccctgtgaac ggcgggagat
    10381 gcaataggtc aggctctcgc taaactcccc aatgtcaagc acttccggaa tcgggagcgc
    10441 ggccgatgca aagtgccgat aaacataacg atctttgtag aaaccatcgg cgcagctatt
    10501 tacccgcagg acatatccac gccctcctac atcgaagctg aaagcacgag attcttcgcc
    10561 ctccgagagc tgcatcaggt cggagacgct gtcgaacttt tcgatcagaa acttctcgac
    10621 agacgtcgcg gtgagttcag gctttttcat atctcattgc cccccggatc tgcgaaagct
    10681 cgagagagat agatttgtag agagagactg gtgatttcag cgtgtcctct ccaaatgaaa
    10741 tgaacttcct tatatagagg aagggtcttg cgaaggatag tgggattgtg cgtcatccct
    10801 tacgtcagtg gagatatcac atcaatccac ttgctttgaa gacgtggttg gaacgtcttc
    10861 tttttccacg atgctcctcg tgggtggggg tccatctttg ggaccactgt cggcagaggc
    10921 atcttgaacg atagcctttc ctttatcgca atgatggcat ttgtaggtgc caccttcctt
    10981 ttctactgtc cttttgatga agtgacagat agctgggcaa tggaatccga ggaggtttcc
    11041 cgatattacc ctttgttgaa aagtctcaat agccctttgg tcttctgaga ctgtatcttt
    11101 gatattcttg gagtagacga gagtgtcgtg ctccaccatg ttggcaagct gctctagcca
    11161 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
    11221 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
    11281 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
    11341 ggataacaat ttcacacagg aaacagctat gacatgatta cgaattcccg atctagtaac
    11401 atagatgaca ccgcgcgcga taatttatcc tagtttgcgc gctatatttt gttttctatc
    11461 gcgtattaaa tgtataattg cgggactcta atcataaaaa cccatctcat aaataacgtc
    11521 atgcattaca tgttaattat tacatgctta acgtaattca acagaaatta tatgataatc
    11581 atcgcaagac cggcaacagg attcaatctt aagaaacttt attgccaaat gtttgaacga
    11641 tcggggaaat tcgagctctc aagcataatc aggcacatca taagggtatc cggcgctgcc
    11701 agcgtag
//
