LOCUS       sequence-455592-       12972 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mapping of cytosol-facing chloroplast outer membrane proximity 
            proteome by proximity-dependent biotinylation in living Arabidopsis 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12972)
  AUTHORS   Bao X, Jia H, Zhang X, Tian S, Zhao Y, Li X, Lin P, Ma C, Wang P, 
            Song CP, Zhu X
  TITLE     Mapping of cytosol-facing organelle outer membrane proximity 
            proteome by proximity-dependent biotinylation in living Arabidopsis 
            cells.
  JOURNAL   Plant J. 2024 Apr;118(1):7-23. doi: 10.1111/tpj.16641. Epub 2024 Jan
            23.
  PUBMED    38261530
REFERENCE   2  (bases 1 to 12972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.16641";
            journalName: "Plant J"; date: "2024-04"; volume: "118"; issue: "1"; 
            pages: "7-23"
COMMENT     Sequence Label: CP2
FEATURES             Location/Qualifiers
     source          1..12972
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(4..69)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(94..120)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(133..159)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(172..198)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(214..1173)
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID 
                     engineered for improved catalytic efficiency (Branon et 
                     al., 2018)"
                     /label=TurboID
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             complement(join(1174..1884,1885..1887,1888..1890))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(1174..1884,1885..1887,1888..1890))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1216..1237)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     1565..1584
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     promoter        complement(2010..2390)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     primer_bind     complement(2924..2941)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2924..2940)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2933..2955)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    3143..3167
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4468..5097
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5526..6599
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      6665..6859
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(7095..7117)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7203..7343
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7258..7277
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7358..7375)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7529..8117)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7609..7628)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9403..10197)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    10622..10646
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    10724..10898
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(10939..11964)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(12037..12382)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(12074..12094)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    12573..12594
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12609..12614,12615..12632,12633..12639)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12647..12663
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12652..12674
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12671..12687
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12671..12687
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      complement(12704..12956)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
ORIGIN
        1 ttacttatcg tcatcgtcct tgtaatcgat gtcgtgatcc ttatagtctc catcatggtc
       61 tttgtagtcc atggtaccgg atccactagt ctaagcataa tcaggcacat cataagggta
      121 tccggcgctg ccagcgtagt caggcacatc ataggggtag gatcctgtgc cggcgtaatc
      181 aggcacgtca tagggataca tgccgctccc tcccttttcg gcagaccgca gactgatttc
      241 tccgcccatc cagggtttga taactccgtc ctgttccagc agcagggctc cctgtttatc
      301 aatcccccgg ctaatcccga aaatctcttt gtccccaatg atcagcttca cgggtctatt
      361 gatgaagtta tccagcttct cccaccgtgg cagatatgga gccaggcctt cctgctcgaa
      421 cagttccagg gctgcccgca gctctcggat cagcgtagcg gccagagtat tcctgtccag
      481 gttaatccct gcttcctgca gtgtgatcca gccctgattg accacgcttt cctccacgcg
      541 cctcatagcc acgttaatcc cggcgccaat gacgatctgt gcagcatcgc ctgttattcc
      601 ggccagctcc accaggatgc ctgccagctt tctatcctgc agatacaggt cattgggcca
      661 tttgactcgc accttgtctg ctcccagctt tcgcagcgct tctgccatga caattccgat
      721 gaccgggccc aggccgattg ctgctggtcc ccgcttcagg cgccaaaaca tactcaggta
      781 caggttagct ccaaaaggag agaaccattt cctccctctg cttcctctcc ctgcctgctg
      841 atattctgca atgcaagcat ccccactctt cagctcgccg attcgatcca gcaggtactg
      901 attggtggag tcgaccacag gcaggactgc cacgctcccg ccgtccagct gtcccagaat
      961 ctgtttagcg ttcagcagcg ggataggctc gggcagagag tagccctttc cgggcacggt
     1021 gaacacgtcc acgccccagt cgcgcagagt ctgaatgtgc ttgttgatag cggccctgga
     1081 catgcccagg gtttctccca gctgttcgcc actatggaac tcgccattag ccaggagagc
     1141 gatcagcttc agaggcacag tattgtcttt catcttgtac agctcgtcca tgccgagagt
     1201 gatcccggcg gcggtcacga actccagcag gaccatgtga tcgcgcttct cgttggggtc
     1261 tttgctcagg gcggactggg tgctcaggta gtggttgtcg ggcagcagca cggggccgtc
     1321 gccgatgggg gtgttctgct ggtagtggtc ggcgagctgc acgctgccgt cctcgatgtt
     1381 gtggcggatc ttgaagttca ccttgatgcc gttcttctgc ttgtcggcca tgatatagac
     1441 gttgtggctg ttgtagttgt actccagctt gtgccccagg atgttgccgt cctccttgaa
     1501 gtcgatgccc ttcagctcga tgcggttcac cagggtgtcg ccctcgaact tcacctcggc
     1561 gcgggtcttg tagttgccgt cgtccttgaa gaagatggtg cgctcctgga cgtagccttc
     1621 gggcatggcg gacttgaaga agtcgtgctg cttcatgtgg tcggggtagc ggctgaagca
     1681 ctgcacgccg taggtcaggg tggtcacgag ggtgggccag ggcacgggca gcttgccggt
     1741 ggtgcagatg aacttcaggg tcagcttgcc gtaggtggca tcgccctcgc cctcgccgga
     1801 cacgctgaac ttgtggccgt ttacgtcgcc gtccagctcg accaggatgg gcaccacccc
     1861 ggtgaacagc tcctcgccct tgctcaccat actagtatct tcacggacgg tcttcttcag
     1921 ctttttggta agcataagaa tcccagcgag acctaaacct agaagaaccc agagattcgc
     1981 agcttgagac gccataagct ttctagagtc gatttggtgt atcgagattg gttatgaaat
     2041 tcagatgcta gtgtaatgta ttggtaattt gggaagatat aataggaagc aaggctattt
     2101 atccatttct gaaaaggcga aatggcgtca ccgcgagcgt cacgcgcatt ccgttcttgc
     2161 tgtaaagcgt tgtttggtac acttttgact agcgaggctt ggcgtgtcag cgtatctatt
     2221 caaaagtcgt taatggctgc ggatcaagaa aaagttggaa tagaaacaga atacccgcga
     2281 aattcaggcc cggttgccat gtcctacacg ccgaaataaa cgaccaaatt agtagaaaaa
     2341 taaaaactga ctcggatact tacgtcacgt cttgcgcact gatttgaaaa atctccctcg
     2401 atcgagaaag agatcaatgt tgagctgctt caaaagcaat gggattgacc agctcgcgga
     2461 tcctacaggc caaattcgct cttagccgta caatattact caccggtgcg atgcccccca
     2521 tcgtaggtga aggtggaaat taatgatcca tcttgagacc acaggcccac aacagctacc
     2581 agtttcctca agggtccacc aaaaacgtaa gcgcttacgt acatggtcga taagaaaagg
     2641 caatttgtag atgttaacat ccaacgtcgc tttcagggat cctacaggcc aaattcgctc
     2701 ttagccgtac aatattactc accggtgcga tgccccccat cgtaggtgaa ggtggaaatt
     2761 aatgatccat cttgagacca caggcccaca acagctacca gtttcctcaa gggtccacca
     2821 aaaacgtaag cgcttacgta catggtcgat aagaaaaggc aatttgtaga tgttaacatc
     2881 caacgtcgct ttcagggatc cgcgagctta tcgataccgt ggcactggcc gtcgttttac
     2941 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     3001 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     3061 gcagcctgaa tggcgaatgc tagagcagct tgagcttgga tcagattgtc gtttcccgcc
     3121 ttcagtttaa actatcagtg tttgacagga tatattggcg ggtaaaccta agagaaaaga
     3181 gcgtttatta gaataatcgg atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat
     3241 ttgtatgtgc atgccaacca cagggttccc ctcgggatca aagtactttg atccaacccc
     3301 tccgctgcta tagtgcagtc ggcttctgac gttcagtgca gccgtcttct gaaaacgaca
     3361 tgtcgcacaa gtcctaagtt acgcgacagg ctgccgccct gcccttttcc tggcgttttc
     3421 ttgtcgcgtg ttttagtcgc ataaagtaga atacttgcga ctagaaccgg agacattacg
     3481 ccatgaacaa gagcgccgcc gctggcctgc tgggctatgc ccgcgtcagc accgacgacc
     3541 aggacttgac caaccaacgg gccgaactgc acgcggccgg ctgcaccaag ctgttttccg
     3601 agaagatcac cggcaccagg cgcgaccgcc cggagctggc caggatgctt gaccacctac
     3661 gccctggcga cgttgtgaca gtgaccaggc tagaccgcct ggcccgcagc acccgcgacc
     3721 tactggacat tgccgagcgc atccaggagg ccggcgcggg cctgcgtagc ctggcagagc
     3781 cgtgggccga caccaccacg ccggccggcc gcatggtgtt gaccgtgttc gccggcattg
     3841 ccgagttcga gcgttcccta atcatcgacc gcacccggag cgggcgcgag gccgccaagg
     3901 cccgaggcgt gaagtttggc ccccgcccta ccctcacccc ggcacagatc gcgcacgccc
     3961 gcgagctgat cgaccaggaa ggccgcaccg tgaaagaggc ggctgcactg cttggcgtgc
     4021 atcgctcgac cctgtaccgc gcacttgagc gcagcgagga agtgacgccc accgaggcca
     4081 ggcggcgcgg tgccttccgt gaggacgcat tgaccgaggc cgacgccctg gcggccgccg
     4141 agaatgaacg ccaagaggaa caagcatgaa accgcaccag gacggccagg acgaaccgtt
     4201 tttcattacc gaagagatcg aggcggagat gatcgcggcc gggtacgtgt tcgagccgcc
     4261 cgcgcacgtc tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg atgccaagct
     4321 ggcggcctgg ccggccagct tggccgctga agaaaccgag cgccgccgtc taaaaaggtg
     4381 atgtgtattt gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga tgcgatgagt
     4441 aaataaacaa atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa ccagaaaggc
     4501 gggtcaggca agacgaccat cgcaacccat ctagcccgcg ccctgcaact cgccggggcc
     4561 gatgttctgt tagtcgattc cgatccccag ggcagtgccc gcgattgggc ggccgtgcgg
     4621 gaagatcaac cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg cgacgtgaag
     4681 gccatcggcc ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc ggacttggct
     4741 gtgtccgcga tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag cccttacgac
     4801 atatgggcca ccgccgacct ggtggagctg gttaagcagc gcattgaggt cacggatgga
     4861 aggctacaag cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat cggcggtgag
     4921 gttgccgagg cgctggccgg gtacgagctg cccattcttg agtcccgtat cacgcagcgc
     4981 gtgagctacc caggcactgc cgccgccggc acaaccgttc ttgaatcaga acccgagggc
     5041 gacgctgccc gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact catttgagtt
     5101 aatgaggtaa agagaaaatg agcaaaagca caaacacgct aagtgccggc cgtccgagcg
     5161 cacgcagcag caaggctgca acgttggcca gcctggcaga cacgccagcc atgaagcggg
     5221 tcaactttca gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg gtacgccaag
     5281 gcaagaccat taccgagctg ctatctgaat acatcgcgca gctaccagag taaatgagca
     5341 aatgaataaa tgagtagatg aattttagcg gctaaaggag gcggcatgga aaatcaagaa
     5401 caaccaggca ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg ttggccaggc
     5461 gtaagcggct gggttgcctg ccggccctgc aatggcactg gaacccccaa gcccgaggaa
     5521 tcggcgtgag cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg
     5581 acctggtgga gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag
     5641 cacgccccgg tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac
     5701 cgccggcagc cggtgcgccg tcgattagga agccgcccaa gggcgacgag caaccagatt
     5761 ttttcgttcc gatgctctat gacgtgggca cccgcgatag tcgcagcatc atggacgtgg
     5821 ccgttttccg tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc tacgagcttc
     5881 cagacgggca cgtagaggtt tccgcagggc cggccggcat ggccagtgtg tgggattacg
     5941 acctggtact gatggcggtt tcccatctaa ccgaatccat gaaccgatac cgggaaggga
     6001 agggagacaa gcccggccgc gtgttccgtc cacacgttgc ggacgtactc aagttctgcc
     6061 ggcgagccga tggcggaaag cagaaagacg acctggtaga aacctgcatt cggttaaaca
     6121 ccacgcacgt tgccatgcag cgtacgaaga aggccaagaa cggccgcctg gtgacggtat
     6181 ccgagggtga agccttgatt agccgctaca agatcgtaaa gagcgaaacc gggcggccgg
     6241 agtacatcga gatcgagcta gctgattgga tgtaccgcga gatcacagaa ggcaagaacc
     6301 cggacgtgct gacggttcac cccgattact ttttgatcga tcccggcatc ggccgttttc
     6361 tctaccgcct ggcacgccgc gccgcaggca aggcagaagc cagatggttg ttcaagacga
     6421 tctacgaacg cagtggcagc gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc
     6481 tgatcgggtc aaatgacctg ccggagtacg atttgaagga ggaggcgggg caggctggcc
     6541 cgatcctagt catgcgctac cgcaacctga tcgagggcga agcatccgcc ggttcctaat
     6601 gtacggagca gatgctaggg caaattgccc tagcagggga aaaaggtcga aaaggtctct
     6661 ttcctgtgga tagcacgtac attgggaacc caaagccgta cattgggaac cggaacccgt
     6721 acattgggaa cccaaagccg tacattggga accggtcaca catgtaagtg actgatataa
     6781 aagagaaaaa aggcgatttt tccgcctaaa actctttaaa acttattaaa actcttaaaa
     6841 cccgcctggc ctgtgcataa ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc
     6901 ctacccttcg gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg
     6961 ctggccgctc aaaaatggct ggcctacggc caggcaatct accagggcgc ggacaagccg
     7021 cgccgtcgcc actcgaccgc cggcgcccac atcaaggcac cctgcctcgc gcgtttcggt
     7081 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     7141 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     7201 ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg
     7261 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
     7321 taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     7381 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     7441 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     7501 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     7561 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     7621 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     7681 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     7741 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     7801 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     7861 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     7921 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     7981 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     8041 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     8101 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     8161 acgaaaactc acgttaagga aggtgcgaac aagtccctga tatgagatca tgtttgtcat
     8221 ctggagccat agaacagggt tcatcatgag tcatcaactt accttcgccg acagtgaatt
     8281 cagcagtaag cgccgtcaga ccagaaaaga gattttcttg tcccgcatgg agcagattct
     8341 gccatggcaa aacatggtgg aagtcatcga gccgttttac cccaaggctg gtaatggccg
     8401 gcgaccttat ccgctggaaa ccatgctacg cattcactgc atgcagcatt ggtacaacct
     8461 gagcgatggc gcgatggaag atgctctgta cgaaatcgcc tccatgcgtc tgtttgcccg
     8521 gttatccctg gatagcgcct tgccggaccg caccaccatc atgaatttcc gccacctgct
     8581 ggagcagcat caactggccc gccaattgtt caagaccatc aatcgctggc tggccgaagc
     8641 aggcgtcatg atgactcaag gcaccttggt cgatgccacc atcattgagg cacccagctc
     8701 gaccaagaac aaagagcagc aacgcgatcc ggagatgcat cagaccaaga aaggcaatca
     8761 gtggcacttt ggcatgaagg cccacattgg tgtcgatgcc aagagtggcc tgacccacag
     8821 cctggtcacc accgcggcca acgagcatga cctcaatcag ctgggtaatc tgctgcatgg
     8881 agaggagcaa tttgtctcag ccgatgccgg ctaccaaggg gcgccacagc gcgaggagct
     8941 ggccgaggtg gatgtggact ggctgatcgc cgagcgcccc ggcaaggtaa gaaccttgaa
     9001 acagcatcca cgcaagaaca aaacggccat caacatcgaa tacatgaaag ccagcatccg
     9061 ggccagggtg gagcacccat ttcgcatcat caagcgacag ttcggcttcg tgaaagccag
     9121 atacaagggg ttgctgaaaa acgataacca actggcgatg ttattcacgc tggccaacct
     9181 gtttcgggcg gaccaaatga tacgtcagtg ggagagatct cactaaaaac tggggataac
     9241 gccttaaatg gcgaagaaac ggtctaaata ggctgattca aggcatttac gggagaaaaa
     9301 atcggctcaa acatgaagaa atgaaatgac tgagtcagcc gagaagaatt tccccgctta
     9361 ttcgcacctt ccttaaggga ttttggtcat gcattctagg tactaaaaca attcatccag
     9421 taaaatataa tattttattt tctcccaatc aggcttgatc cccagtaagt caaaaaatag
     9481 ctcgacatac tgttcttccc cgatatcctc cctgatcgac cggacgcaga aggcaatgtc
     9541 ataccacttg tccgccctgc cgcttctccc aagatcaata aagccactta ctttgccatc
     9601 tttcacaaag atgttgctgt ctcccaggtc gccgtgggaa aagacaagtt cctcttcggg
     9661 cttttccgtc tttaaaaaat catacagctc gcgcggatct ttaaatggag tgtcttcttc
     9721 ccagttttcg caatccacat cggccagatc gttattcagt aagtaatcca attcggctaa
     9781 gcggctgtct aagctattcg tatagggaca atccgatatg tcgatggagt gaaagagcct
     9841 gatgcactcc gcatacagct cgataatctt ttcagggctt tgttcatctt catactcttc
     9901 cgagcaaagg acgccatcgg cctcactcat gagcagattg ctccagccat catgccgttc
     9961 aaagtgcagg acctttggaa caggcagctt tccttccagc catagcatca tgtccttttc
    10021 ccgttccaca tcataggtgg tccctttata ccggctgtcc gtcattttta aatataggtt
    10081 ttcattttct cccaccagct tatatacctt agcaggagac attccttccg tatcttttac
    10141 gcagcggtat ttttcgatca gttttttcaa ttccggtgat attctcattt tagccattta
    10201 ttatttcctt cctcttttct acagtattta aagatacccc aagaagctaa ttataacaag
    10261 acgaactcca attcactgtt ccttgcattc taaaacctta aataccagaa aacagctttt
    10321 tcaaagttgt tttcaaagtt ggcgtataac atagtatcga cggagccgat tttgaaaccg
    10381 cggtgatcac aggcagcaac gctctgtcat cgttacaatc aacatgctac cctccgcgag
    10441 atcatccgtg tttcaaaccc ggcagcttag ttgccgttct tccgaatagc atcggtaaca
    10501 tgagcaaagt ctgccgcctt acaacggctc tcccgctgac gccgtcccgg actgatgggc
    10561 tgcctgtatc gagtggtgat tttgtgccga gctgccggtc ggggagctgt tggctggctg
    10621 gtggcaggat atattgtggt gtaaacaaat tgacgcttag acaacttaat aacacattgc
    10681 ggacgttttt aatgtactga attaacgccg aattaattcg ggggatctgg attttagtac
    10741 tggattttgg ttttaggaat tagaaatttt attgatagaa gtattttaca aatacaaata
    10801 catactaagg gtttcttata tgctcaacac atgagcgaaa ccctatagga accctaattc
    10861 ccttatctgg gaactactca cacattatta tggagaaact cgagcttgtc gatcgacaga
    10921 tcccggtcgg catctactct atttctttgc cctcggacga gtgctggggc gtcggtttcc
    10981 actatcggcg agtacttcta cacagccatc ggtccagacg gccgcgcttc tgcgggcgat
    11041 ttgtgtacgc ccgacagtcc cggctccgga tcggacgatt gcgtcgcatc gaccctgcgc
    11101 ccaagctgca tcatcgaaat tgccgtcaac caagctctga tagagttggt caagaccaat
    11161 gcggagcata tacgcccgga gtcgtggcga tcctgcaagc tccggatgcc tccgctcgaa
    11221 gtagcgcgtc tgctgctcca tacaagccaa ccacggcctc cagaagaaga tgttggcgac
    11281 ctcgtattgg gaatccccga acatcgcctc gctccagtca atgaccgctg ttatgcggcc
    11341 attgtccgtc aggacattgt tggagccgaa atccgcgtgc acgaggtgcc ggacttcggg
    11401 gcagtcctcg gcccaaagca tcagctcatc gagagcctgc gcgacggacg cactgacggt
    11461 gtcgtccatc acagtttgcc agtgatacac atggggatca gcaatcgcgc atatgaaatc
    11521 acgccatgta gtgtattgac cgattccttg cggtccgaat gggccgaacc cgctcgtctg
    11581 gctaagatcg gccgcagcga tcgcatccat agcctccgcg accggttgta gaacagcggg
    11641 cagttcggtt tcaggcaggt cttgcaacgt gacaccctgt gaacggcggg agatgcaata
    11701 ggtcaggctc tcgctaaact ccccaatgtc aagcacttcc ggaatcggga gcgcggccga
    11761 tgcaaagtgc cgataaacat aacgatcttt gtagaaacca tcggcgcagc tatttacccg
    11821 caggacatat ccacgccctc ctacatcgaa gctgaaagca cgagattctt cgccctccga
    11881 gagctgcatc aggtcggaga cgctgtcgaa cttttcgatc agaaacttct cgacagacgt
    11941 cgcggtgagt tcaggctttt tcatatctca ttgccccccg gatctgcgaa agctcgagag
    12001 agatagattt gtagagagag actggtgatt tcagcgtgtc ctctccaaat gaaatgaact
    12061 tccttatata gaggaagggt cttgcgaagg atagtgggat tgtgcgtcat cccttacgtc
    12121 agtggagata tcacatcaat ccacttgctt tgaagacgtg gttggaacgt cttctttttc
    12181 cacgatgctc ctcgtgggtg ggggtccatc tttgggacca ctgtcggcag aggcatcttg
    12241 aacgatagcc tttcctttat cgcaatgatg gcatttgtag gtgccacctt ccttttctac
    12301 tgtccttttg atgaagtgac agatagctgg gcaatggaat ccgaggaggt ttcccgatat
    12361 taccctttgt tgaaaagtct caatagccct ttggtcttct gagactgtat ctttgatatt
    12421 cttggagtag acgagagtgt cgtgctccac catgttggca agctgctcta gccaatacgc
    12481 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
    12541 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
    12601 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
    12661 caatttcaca caggaaacag ctatgacatg attacgaatt cccgatctag taacatagat
    12721 gacaccgcgc gcgataattt atcctagttt gcgcgctata ttttgttttc tatcgcgtat
    12781 taaatgtata attgcgggac tctaatcata aaaacccatc tcataaataa cgtcatgcat
    12841 tacatgttaa ttattacatg cttaacgtaa ttcaacagaa attatatgat aatcatcgca
    12901 agaccggcaa caggattcaa tcttaagaaa ctttattgcc aaatgtttga acgatcgggg
    12961 aaattcgagc tc
//
