LOCUS       sequence-455593-       12981 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mapping of cytosol-facing mitochondrion outer membrane proximity 
            proteome by proximity-dependent biotinylation in living Arabidopsis 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12981)
  AUTHORS   Bao X, Jia H, Zhang X, Tian S, Zhao Y, Li X, Lin P, Ma C, Wang P, 
            Song CP, Zhu X
  TITLE     Mapping of cytosol-facing organelle outer membrane proximity 
            proteome by proximity-dependent biotinylation in living Arabidopsis 
            cells.
  JOURNAL   Plant J. 2024 Apr;118(1):7-23. doi: 10.1111/tpj.16641. Epub 2024 Jan
            23.
  PUBMED    38261530
REFERENCE   2  (bases 1 to 12981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.16641";
            journalName: "Plant J"; date: "2024-04"; volume: "118"; issue: "1"; 
            pages: "7-23"
COMMENT     Sequence Label: MT2
FEATURES             Location/Qualifiers
     source          1..12981
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(4..69)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(join(211..921,922..924))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             complement(join(211..921,922..924))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(253..274)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     602..621
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             complement(925..1884)
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID 
                     engineered for improved catalytic efficiency (Branon et 
                     al., 2018)"
                     /label=TurboID
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             complement(1897..1923)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(1936..1962)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(1975..2001)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(2019..2399)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     primer_bind     complement(2933..2950)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2933..2949)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2942..2964)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    3152..3176
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4477..5106
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5535..6608
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      6674..6868
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(7104..7126)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7212..7352
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7267..7286
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7367..7384)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7538..8126)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7618..7637)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9412..10206)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    10631..10655
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    10733..10907
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(10948..11973)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(12046..12391)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(12083..12103)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    12582..12603
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12618..12623,12624..12641,12642..12648)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12656..12672
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12661..12683
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12680..12696
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12680..12696
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      complement(12713..12965)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
ORIGIN
        1 ttacttatcg tcatcgtcct tgtaatcgat gtcgtgatcc ttatagtctc catcatggtc
       61 tttgtagtcc atggtaccgg atccactagt tcagagctgc tttcggtatc tcacgaaggc
      121 ccaaactgct accagggtaa gggcaaacac tggcagcagc accacagcta caggaactcc
      181 actaggctct ccgtcgcctc gatgccgcgg cttgtacagc tcgtccatgc cgagagtgat
      241 cccggcggcg gtcacgaact ccagcaggac catgtgatcg cgcttctcgt tggggtcttt
      301 gctcagggcg gactgggtgc tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc
      361 gatgggggtg ttctgctggt agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg
      421 gcggatcttg aagttcacct tgatgccgtt cttctgcttg tcggccatga tatagacgtt
      481 gtggctgttg tagttgtact ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc
      541 gatgcccttc agctcgatgc ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg
      601 ggtcttgtag ttgccgtcgt ccttgaagaa gatggtgcgc tcctggacgt agccttcggg
      661 catggcggac ttgaagaagt cgtgctgctt catgtggtcg gggtagcggc tgaagcactg
      721 cacgccgtag gtcagggtgg tcacgagggt gggccagggc acgggcagct tgccggtggt
      781 gcagatgaac ttcagggtca gcttgccgta ggtggcatcg ccctcgccct cgccggacac
      841 gctgaacttg tggccgttta cgtcgccgtc cagctcgacc aggatgggca ccaccccggt
      901 gaacagctcc tcgcccttgc tcaccttttc ggcagaccgc agactgattt ctccgcccat
      961 ccagggtttg ataactccgt cctgttccag cagcagggct ccctgtttat caatcccccg
     1021 gctaatcccg aaaatctctt tgtccccaat gatcagcttc acgggtctat tgatgaagtt
     1081 atccagcttc tcccaccgtg gcagatatgg agccaggcct tcctgctcga acagttccag
     1141 ggctgcccgc agctctcgga tcagcgtagc ggccagagta ttcctgtcca ggttaatccc
     1201 tgcttcctgc agtgtgatcc agccctgatt gaccacgctt tcctccacgc gcctcatagc
     1261 cacgttaatc ccggcgccaa tgacgatctg tgcagcatcg cctgttattc cggccagctc
     1321 caccaggatg cctgccagct ttctatcctg cagatacagg tcattgggcc atttgactcg
     1381 caccttgtct gctcccagct ttcgcagcgc ttctgccatg acaattccga tgaccgggcc
     1441 caggccgatt gctgctggtc cccgcttcag gcgccaaaac atactcaggt acaggttagc
     1501 tccaaaagga gagaaccatt tcctccctct gcttcctctc cctgcctgct gatattctgc
     1561 aatgcaagca tccccactct tcagctcgcc gattcgatcc agcaggtact gattggtgga
     1621 gtcgaccaca ggcaggactg ccacgctccc gccgtccagc tgtcccagaa tctgtttagc
     1681 gttcagcagc gggataggct cgggcagaga gtagcccttt ccgggcacgg tgaacacgtc
     1741 cacgccccag tcgcgcagag tctgaatgtg cttgttgata gcggccctgg acatgcccag
     1801 ggtttctccc agctgttcgc cactatggaa ctcgccatta gccaggagag cgatcagctt
     1861 cagaggcaca gtattgtctt tcatgccgct ccctccagca taatcaggca catcataagg
     1921 gtatccggcg ctgccagcgt agtcaggcac atcatagggg taggatcctg tgccggcgta
     1981 atcaggcacg tcatagggat acataagctt tctagagtcg atttggtgta tcgagattgg
     2041 ttatgaaatt cagatgctag tgtaatgtat tggtaatttg ggaagatata ataggaagca
     2101 aggctattta tccatttctg aaaaggcgaa atggcgtcac cgcgagcgtc acgcgcattc
     2161 cgttcttgct gtaaagcgtt gtttggtaca cttttgacta gcgaggcttg gcgtgtcagc
     2221 gtatctattc aaaagtcgtt aatggctgcg gatcaagaaa aagttggaat agaaacagaa
     2281 tacccgcgaa attcaggccc ggttgccatg tcctacacgc cgaaataaac gaccaaatta
     2341 gtagaaaaat aaaaactgac tcggatactt acgtcacgtc ttgcgcactg atttgaaaaa
     2401 tctccctcga tcgagaaaga gatcaatgtt gagctgcttc aaaagcaatg ggattgacca
     2461 gctcgcggat cctacaggcc aaattcgctc ttagccgtac aatattactc accggtgcga
     2521 tgccccccat cgtaggtgaa ggtggaaatt aatgatccat cttgagacca caggcccaca
     2581 acagctacca gtttcctcaa gggtccacca aaaacgtaag cgcttacgta catggtcgat
     2641 aagaaaaggc aatttgtaga tgttaacatc caacgtcgct ttcagggatc ctacaggcca
     2701 aattcgctct tagccgtaca atattactca ccggtgcgat gccccccatc gtaggtgaag
     2761 gtggaaatta atgatccatc ttgagaccac aggcccacaa cagctaccag tttcctcaag
     2821 ggtccaccaa aaacgtaagc gcttacgtac atggtcgata agaaaaggca atttgtagat
     2881 gttaacatcc aacgtcgctt tcagggatcc gcgagcttat cgataccgtg gcactggccg
     2941 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     3001 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     3061 aacagttgcg cagcctgaat ggcgaatgct agagcagctt gagcttggat cagattgtcg
     3121 tttcccgcct tcagtttaaa ctatcagtgt ttgacaggat atattggcgg gtaaacctaa
     3181 gagaaaagag cgtttattag aataatcgga tatttaaaag ggcgtgaaaa ggtttatccg
     3241 ttcgtccatt tgtatgtgca tgccaaccac agggttcccc tcgggatcaa agtactttga
     3301 tccaacccct ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag ccgtcttctg
     3361 aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg cccttttcct
     3421 ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac tagaaccgga
     3481 gacattacgc catgaacaag agcgccgccg ctggcctgct gggctatgcc cgcgtcagca
     3541 ccgacgacca ggacttgacc aaccaacggg ccgaactgca cgcggccggc tgcaccaagc
     3601 tgttttccga gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc aggatgcttg
     3661 accacctacg ccctggcgac gttgtgacag tgaccaggct agaccgcctg gcccgcagca
     3721 cccgcgacct actggacatt gccgagcgca tccaggaggc cggcgcgggc ctgcgtagcc
     3781 tggcagagcc gtgggccgac accaccacgc cggccggccg catggtgttg accgtgttcg
     3841 ccggcattgc cgagttcgag cgttccctaa tcatcgaccg cacccggagc gggcgcgagg
     3901 ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac cctcaccccg gcacagatcg
     3961 cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg gctgcactgc
     4021 ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa gtgacgccca
     4081 ccgaggccag gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc gacgccctgg
     4141 cggccgccga gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg acggccagga
     4201 cgaaccgttt ttcattaccg aagagatcga ggcggagatg atcgcggccg ggtacgtgtt
     4261 cgagccgccc gcgcacgtct caaccgtgcg gctgcatgaa atcctggccg gtttgtctga
     4321 tgccaagctg gcggcctggc cggccagctt ggccgctgaa gaaaccgagc gccgccgtct
     4381 aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg cgtatatgat
     4441 gcgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc tgtacttaac
     4501 cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc cctgcaactc
     4561 gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg cgattgggcg
     4621 gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac gattgaccgc
     4681 gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc ccaggcggcg
     4741 gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt gcagccaagc
     4801 ccttacgaca tatgggccac cgccgacctg gtggagctgg ttaagcagcg cattgaggtc
     4861 acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg cacgcgcatc
     4921 ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga gtcccgtatc
     4981 acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct tgaatcagaa
     5041 cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa atcaaaactc
     5101 atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta agtgccggcc
     5161 gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac acgccagcca
     5221 tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag atgtacgcgg
     5281 tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag ctaccagagt
     5341 aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg cggcatggaa
     5401 aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg aacgggcggt
     5461 tggccaggcg taagcggctg ggttgcctgc cggccctgca atggcactgg aacccccaag
     5521 cccgaggaat cggcgtgagc ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc
     5581 tgggtgatga cctggtggag aagttgaagg ccgcgcaggc cgcccagcgg caacgcatcg
     5641 aggcagaagc acgccccggt gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat
     5701 cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc
     5761 aaccagattt tttcgttccg atgctctatg acgtgggcac ccgcgatagt cgcagcatca
     5821 tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct
     5881 acgagcttcc agacgggcac gtagaggttt ccgcagggcc ggccggcatg gccagtgtgt
     5941 gggattacga cctggtactg atggcggttt cccatctaac cgaatccatg aaccgatacc
     6001 gggaagggaa gggagacaag cccggccgcg tgttccgtcc acacgttgcg gacgtactca
     6061 agttctgccg gcgagccgat ggcggaaagc agaaagacga cctggtagaa acctgcattc
     6121 ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg
     6181 tgacggtatc cgagggtgaa gccttgatta gccgctacaa gatcgtaaag agcgaaaccg
     6241 ggcggccgga gtacatcgag atcgagctag ctgattggat gtaccgcgag atcacagaag
     6301 gcaagaaccc ggacgtgctg acggttcacc ccgattactt tttgatcgat cccggcatcg
     6361 gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt
     6421 tcaagacgat ctacgaacgc agtggcagcg ccggagagtt caagaagttc tgtttcaccg
     6481 tgcgcaagct gatcgggtca aatgacctgc cggagtacga tttgaaggag gaggcggggc
     6541 aggctggccc gatcctagtc atgcgctacc gcaacctgat cgagggcgaa gcatccgccg
     6601 gttcctaatg tacggagcag atgctagggc aaattgccct agcaggggaa aaaggtcgaa
     6661 aaggtctctt tcctgtggat agcacgtaca ttgggaaccc aaagccgtac attgggaacc
     6721 ggaacccgta cattgggaac ccaaagccgt acattgggaa ccggtcacac atgtaagtga
     6781 ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa
     6841 ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc gaagagctgc
     6901 aaaaagcgcc tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg cgtcggccta
     6961 tcgcggccgc tggccgctca aaaatggctg gcctacggcc aggcaatcta ccagggcgcg
     7021 gacaagccgc gccgtcgcca ctcgaccgcc ggcgcccaca tcaaggcacc ctgcctcgcg
     7081 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
     7141 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
     7201 gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta
     7261 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
     7321 acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact
     7381 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     7441 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     7501 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     7561 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     7621 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     7681 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     7741 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     7801 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     7861 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     7921 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     7981 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     8041 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     8101 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     8161 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gcattctagg gaaggtgcga
     8221 acaagtccct gatatgagat catgtttgtc atctggagcc atagaacagg gttcatcatg
     8281 agtcatcaac ttaccttcgc cgacagtgaa ttcagcagta agcgccgtca gaccagaaaa
     8341 gagattttct tgtcccgcat ggagcagatt ctgccatggc aaaacatggt ggaagtcatc
     8401 gagccgtttt accccaaggc tggtaatggc cggcgacctt atccgctgga aaccatgcta
     8461 cgcattcact gcatgcagca ttggtacaac ctgagcgatg gcgcgatgga agatgctctg
     8521 tacgaaatcg cctccatgcg tctgtttgcc cggttatccc tggatagcgc cttgccggac
     8581 cgcaccacca tcatgaattt ccgccacctg ctggagcagc atcaactggc ccgccaattg
     8641 ttcaagacca tcaatcgctg gctggccgaa gcaggcgtca tgatgactca aggcaccttg
     8701 gtcgatgcca ccatcattga ggcacccagc tcgaccaaga acaaagagca gcaacgcgat
     8761 ccggagatgc atcagaccaa gaaaggcaat cagtggcact ttggcatgaa ggcccacatt
     8821 ggtgtcgatg ccaagagtgg cctgacccac agcctggtca ccaccgcggc caacgagcat
     8881 gacctcaatc agctgggtaa tctgctgcat ggagaggagc aatttgtctc agccgatgcc
     8941 ggctaccaag gggcgccaca gcgcgaggag ctggccgagg tggatgtgga ctggctgatc
     9001 gccgagcgcc ccggcaaggt aagaaccttg aaacagcatc cacgcaagaa caaaacggcc
     9061 atcaacatcg aatacatgaa agccagcatc cgggccaggg tggagcaccc atttcgcatc
     9121 atcaagcgac agttcggctt cgtgaaagcc agatacaagg ggttgctgaa aaacgataac
     9181 caactggcga tgttattcac gctggccaac ctgtttcggg cggaccaaat gatacgtcag
     9241 tgggagagat ctcactaaaa actggggata acgccttaaa tggcgaagaa acggtctaaa
     9301 taggctgatt caaggcattt acgggagaaa aaatcggctc aaacatgaag aaatgaaatg
     9361 actgagtcag ccgagaagaa tttccccgct tattcgcacc ttccctaggt actaaaacaa
     9421 ttcatccagt aaaatataat attttatttt ctcccaatca ggcttgatcc ccagtaagtc
     9481 aaaaaatagc tcgacatact gttcttcccc gatatcctcc ctgatcgacc ggacgcagaa
     9541 ggcaatgtca taccacttgt ccgccctgcc gcttctccca agatcaataa agccacttac
     9601 tttgccatct ttcacaaaga tgttgctgtc tcccaggtcg ccgtgggaaa agacaagttc
     9661 ctcttcgggc ttttccgtct ttaaaaaatc atacagctcg cgcggatctt taaatggagt
     9721 gtcttcttcc cagttttcgc aatccacatc ggccagatcg ttattcagta agtaatccaa
     9781 ttcggctaag cggctgtcta agctattcgt atagggacaa tccgatatgt cgatggagtg
     9841 aaagagcctg atgcactccg catacagctc gataatcttt tcagggcttt gttcatcttc
     9901 atactcttcc gagcaaagga cgccatcggc ctcactcatg agcagattgc tccagccatc
     9961 atgccgttca aagtgcagga cctttggaac aggcagcttt ccttccagcc atagcatcat
    10021 gtccttttcc cgttccacat cataggtggt ccctttatac cggctgtccg tcatttttaa
    10081 atataggttt tcattttctc ccaccagctt atatacctta gcaggagaca ttccttccgt
    10141 atcttttacg cagcggtatt tttcgatcag ttttttcaat tccggtgata ttctcatttt
    10201 agccatttat tatttccttc ctcttttcta cagtatttaa agatacccca agaagctaat
    10261 tataacaaga cgaactccaa ttcactgttc cttgcattct aaaaccttaa ataccagaaa
    10321 acagcttttt caaagttgtt ttcaaagttg gcgtataaca tagtatcgac ggagccgatt
    10381 ttgaaaccgc ggtgatcaca ggcagcaacg ctctgtcatc gttacaatca acatgctacc
    10441 ctccgcgaga tcatccgtgt ttcaaacccg gcagcttagt tgccgttctt ccgaatagca
    10501 tcggtaacat gagcaaagtc tgccgcctta caacggctct cccgctgacg ccgtcccgga
    10561 ctgatgggct gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt
    10621 ggctggctgg tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata
    10681 acacattgcg gacgttttta atgtactgaa ttaacgccga attaattcgg gggatctgga
    10741 ttttagtact ggattttggt tttaggaatt agaaatttta ttgatagaag tattttacaa
    10801 atacaaatac atactaaggg tttcttatat gctcaacaca tgagcgaaac cctataggaa
    10861 ccctaattcc cttatctggg aactactcac acattattat ggagaaactc gagcttgtcg
    10921 atcgacagat cccggtcggc atctactcta tttctttgcc ctcggacgag tgctggggcg
    10981 tcggtttcca ctatcggcga gtacttctac acagccatcg gtccagacgg ccgcgcttct
    11041 gcgggcgatt tgtgtacgcc cgacagtccc ggctccggat cggacgattg cgtcgcatcg
    11101 accctgcgcc caagctgcat catcgaaatt gccgtcaacc aagctctgat agagttggtc
    11161 aagaccaatg cggagcatat acgcccggag tcgtggcgat cctgcaagct ccggatgcct
    11221 ccgctcgaag tagcgcgtct gctgctccat acaagccaac cacggcctcc agaagaagat
    11281 gttggcgacc tcgtattggg aatccccgaa catcgcctcg ctccagtcaa tgaccgctgt
    11341 tatgcggcca ttgtccgtca ggacattgtt ggagccgaaa tccgcgtgca cgaggtgccg
    11401 gacttcgggg cagtcctcgg cccaaagcat cagctcatcg agagcctgcg cgacggacgc
    11461 actgacggtg tcgtccatca cagtttgcca gtgatacaca tggggatcag caatcgcgca
    11521 tatgaaatca cgccatgtag tgtattgacc gattccttgc ggtccgaatg ggccgaaccc
    11581 gctcgtctgg ctaagatcgg ccgcagcgat cgcatccata gcctccgcga ccggttgtag
    11641 aacagcgggc agttcggttt caggcaggtc ttgcaacgtg acaccctgtg aacggcggga
    11701 gatgcaatag gtcaggctct cgctaaactc cccaatgtca agcacttccg gaatcgggag
    11761 cgcggccgat gcaaagtgcc gataaacata acgatctttg tagaaaccat cggcgcagct
    11821 atttacccgc aggacatatc cacgccctcc tacatcgaag ctgaaagcac gagattcttc
    11881 gccctccgag agctgcatca ggtcggagac gctgtcgaac ttttcgatca gaaacttctc
    11941 gacagacgtc gcggtgagtt caggcttttt catatctcat tgccccccgg atctgcgaaa
    12001 gctcgagaga gatagatttg tagagagaga ctggtgattt cagcgtgtcc tctccaaatg
    12061 aaatgaactt ccttatatag aggaagggtc ttgcgaagga tagtgggatt gtgcgtcatc
    12121 ccttacgtca gtggagatat cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc
    12181 ttctttttcc acgatgctcc tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga
    12241 ggcatcttga acgatagcct ttcctttatc gcaatgatgg catttgtagg tgccaccttc
    12301 cttttctact gtccttttga tgaagtgaca gatagctggg caatggaatc cgaggaggtt
    12361 tcccgatatt accctttgtt gaaaagtctc aatagccctt tggtcttctg agactgtatc
    12421 tttgatattc ttggagtaga cgagagtgtc gtgctccacc atgttggcaa gctgctctag
    12481 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
    12541 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
    12601 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
    12661 agcggataac aatttcacac aggaaacagc tatgacatga ttacgaattc ccgatctagt
    12721 aacatagatg acaccgcgcg cgataattta tcctagtttg cgcgctatat tttgttttct
    12781 atcgcgtatt aaatgtataa ttgcgggact ctaatcataa aaacccatct cataaataac
    12841 gtcatgcatt acatgttaat tattacatgc ttaacgtaat tcaacagaaa ttatatgata
    12901 atcatcgcaa gaccggcaac aggattcaat cttaagaaac tttattgcca aatgtttgaa
    12961 cgatcgggga aattcgagct c
//
