LOCUS       sequence-455602-       13448 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EcTrp aaRS/tRNA system for TGA suppression with EGFP containing two 
            stop codons for dual suppression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13448)
  AUTHORS   Osgood AO, Zheng Y, Roy SJS, Biris N, Hussain M, Loynd C, Jewel D, 
            Italia JS, Chatterjee A
  TITLE     An Efficient Opal-Suppressor Tryptophanyl Pair Creates New Routes 
            for Simultaneously Incorporating up to Three Distinct Noncanonical 
            Amino Acids into Proteins in Mammalian Cells.
  JOURNAL   Angew Chem Int Ed Engl. 2023 May 2;62(19):e202219269. doi: 
            10.1002/anie.202219269. Epub 2023 Apr 12.
  PUBMED    36905325
REFERENCE   2  (bases 1 to 13448)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Angew Chem 
            Int Ed Engl."; date: "2023-05-2"; volume: "62(19):e202219269. doi"; 
            pages: " 10.1002/anie.202219269. Epub 2023 Apr 12"
COMMENT     Sequence Label: pAcBac3-EcTrp-TGA-EGFP**
FEATURES             Location/Qualifiers
     source          1..13448
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      102..556
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(189..208)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     398..419
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        582..686
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(687..755,756..1547)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(905..924)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1718..2306
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2207..2226
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2460..2477
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  2611..2835
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2902..3435)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3624..3629,3630..3646,3647..3652))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     polyA_signal    4082..4306
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     4295..4312
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     primer_bind     complement(5406..5425)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5407..5425)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(5470..5673)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(5499..5519)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5674..5977
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6051..6299
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     promoter        6399..6647
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     promoter        6747..6995
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     promoter        7095..7343
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     enhancer        7578..7881
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          8161..9177
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     9101..9123
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     9185..9204
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      9241..9250
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(9247..9249,9250..9252,9253..9963)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDAT*GKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNV*IMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9292..9313)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9553..9572)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9900..9921
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             9973..10002
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             10018..10047
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             10021..10050
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             10024..10053
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     misc_feature    10092..10680
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10145..10165)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10563..10574)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(10748..10767)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    10813..10868
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(10867..10886)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     promoter        11220..11311
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     11236..11253
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             join(11370..11417,11418..12905)
                     /codon_start=1
                     /product="vesicular stomatitis virus G glycoprotein"
                     /label=VSV-G
                     /note="Indiana strain"
                     /translation="MKCLLYLAFLFIGVNCKFTIVFPHNQKGNWKNVPSNYHYCPSSSD
                     LNWHNDLIGTALQVKMPKSHKAIQADGWMCHASKWVTTCDFRWYGPKYITHSIRSFTPS
                     VEQCKESIEQTKQGTWLNPGFPPQSCGYATVTDAEAVIVQVTPHHVLVDEYTGEWVDSQ
                     FINGKCSNYICPTVHNSTTWHSDYKVKGLCDSNLISMDITFFSEDGELSSLGKEGTGFR
                     SNYFAYETGGKACKMQYCKHWGVRLPSGVWFEMADKDLFAAARFPECPEGSSISAPSQT
                     SVDVSLIQDVERILDYSLCQETWSKIRAGLPISPVDLSYLAPKNPGTGPAFTIINGTLK
                     YFETRYIRVDIAAPILSRMVGMISGTTTERELWDDWAPYEDVEIGPNGVLRTSSGYKFP
                     LYMIGHGMLDSDLHLSSKAQVFEHPHIQDAASQLPDDESLFFGDTGLSKNPIELVEGWF
                     SSWKSSIASFFFIIGLIIGLFLVLRVGIHLCIKLKHTKKRQIYTDIEMNRLGK"
     polyA_signal    13038..13172
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13075..13094)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     13129..13148
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  13201..13366
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga
       61 ctgaatatca acgcttattt gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
      121 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
      181 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
      241 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
      301 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
      361 ttttcgccct ttgacgttgg agtccacgtt cttaatagtg gactcttgtt ccaaactgga
      421 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
      481 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
      541 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
      601 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
      661 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
      721 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
      781 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
      841 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
      901 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
      961 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     1021 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     1081 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     1141 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     1201 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     1261 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     1321 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     1381 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     1441 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     1501 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     1561 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     1621 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     1681 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     1741 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     1801 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     1861 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     1921 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     1981 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     2041 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     2101 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     2161 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     2221 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     2281 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     2341 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     2401 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     2461 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc
     2521 tgtgcggtat ttcacaccgc atagaccagc cgcgtaacct ggcaaaatcg gttacggttg
     2581 agtaataaat ggatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa
     2641 caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga
     2701 aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact
     2761 cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg
     2821 gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc
     2881 gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac
     2941 ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc
     3001 ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttgagga gattgatgag
     3061 cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat
     3121 ctcactacgc ggctgctcaa acttgggcag aacgtaagcc gcgagagcgc caacaaccgc
     3181 ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta
     3241 atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag
     3301 atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat
     3361 gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt
     3421 gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg
     3481 cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga
     3541 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg
     3601 agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc
     3661 gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg
     3721 aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg
     3781 cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg cccttgcttc
     3841 aggagatcgg tagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag
     3901 tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag gactctagct
     3961 atagttctag tggttggcta cgtacccgta gtggctatgg cagggcttgc gcttaatgcg
     4021 ccgctacagg gcgcgtgggg atacccccta gagccccagc tggttctttc cgcctcagaa
     4081 gccatagagc ccaccgcatc cccagcatgc ctgctattgt cttcccaatc ctcccccttg
     4141 ctgtcctgcc ccaccccacc ccccagaata gaatgacacc tactcagaca atgcgatgca
     4201 atttcctcat tttattagga aaggacagtg ggagtggcac cttccagggt caaggaaggc
     4261 acgggggagg ggcaaacaac agatggctgg caactagaag gcacagtcga ggctgatcag
     4321 cgggtttaaa cgggccctct agactcgagt taaagtcgac ttaacgcgtt gaattcttac
     4381 ggcttcgcca caaaaccaat cgcttcgtac accgctttta gcgtacggga agcgtgcgcg
     4441 ctggcttttt ccgcgccatc tttcatcacc tgttgcagga aggcttcatc gttgcggaaa
     4501 cggtgatagc gttcctgcaa ttcagtcagc ataccggaaa cggcatcagc cacttcacct
     4561 ttcagatgac catacatctt gccttcgaac tgtttttcca gttctgggat gctctggccc
     4621 gttaccgctg aaaggatatc caacaggttg gaaacgcccg ctttgttctg cacatcgtag
     4681 cgaactaccg gcggctcgtc ggagtcagtg accgcacgtt tgattttctt cactaccgat
     4741 ttcggatctt ccagcaggcc gataacgtta ttgcgattat cgtcagactt ggacatcttc
     4801 ttggtcggct ccagcagcga cattacgcgc gcgccagatt tcggaataaa cggctccggc
     4861 accttaaaga tctcgccata cagcgcgttg aaacgctggg caatatcgcg gctcagttcg
     4921 aggtgctgtt tctggtcttc accacaagga cccagattag tttgatacag caggatgtcc
     4981 gctgccatca gcaccggata gtcaaacaga ccagcgttga tgttctcggc ataacgcgca
     5041 gatttatctt taaactgcgt catgcgactc agttcgccga agtaggtata gcagttcagt
     5101 gcccagccta actgtgcatg ttccggcacg tgggactgaa caaaaatggt gcttttctca
     5161 ggatcgatac cacaagccag atacaaggcc agcgtatcca gcgtcgcttt acgcagcttc
     5221 tgtgcatcct ggcgcacggt gatcgcgtgt tggtcaacga tacagtaaat gcaatggtag
     5281 tcatcctgca tgtttaccca ctgacgcagc gcacccatgt agttaccaat ggtcaattca
     5341 cctgagggct gtgcgccagc aaaaacgatg ggcttagtca tggtggcgct agccagcttg
     5401 ggtctcccta tagtgagtcg tattaatttc gataagccag taagcagtgg gttctctagt
     5461 tagccagaga gctctgctta tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt
     5521 caatggggcg gagttgttac gacattttgg aaagtcccgt tgattttggt gccaaaacaa
     5581 actcccattg acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa accgctatcc
     5641 acgcccattg atgtactgcc aaaaccgcat caccatggta atagcgatga ctaatacgta
     5701 gatgtactgc caagtaggaa agtcccataa ggtcatgtac tgggcataat gccaggcggg
     5761 ccatttaccg tcattgacgt caataggggg cgtacttggc atatgataca cttgatgtac
     5821 tgccaagtgg gcagtttacc gtaaatactc cacccattga cgtcaatgga aagtccctat
     5881 tggcgttact atgggaacat acgtcattat tgacgtcaat gggcgggggt cgttgggcgg
     5941 tcagccaggc gggccattta ccgtaagtta tgtaacgcgg aactccatat atgggctatg
     6001 aactaatgac cccgtaattg attactatta ataactaggt cgggcaggaa gagggcctat
     6061 ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag ataattagaa
     6121 ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga aagtaataat
     6181 ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat atgcttaccg
     6241 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgaaacacca
     6301 ggggcgtagt tcaattggta gagcaccggt cttcaaaacc gggtgttggg agttcgagtc
     6361 tctccgcccc tgccattttt tgctaggtcg ggcaggaaga gggcctattt cccatgattc
     6421 cttcatattt gcatatacga tacaaggctg ttagagagat aattagaatt aatttgactg
     6481 taaacacaaa gatattagta caaaatacgt gacgtagaaa gtaataattt cttgggtagt
     6541 ttgcagtttt aaaattatgt tttaaaatgg actatcatat gcttaccgta acttgaaagt
     6601 atttcgattt cttggcttta tatatcttgt ggaaaggacg aaacaccagg ggcgtagttc
     6661 aattggtaga gcaccggtct tcaaaaccgg gtgttgggag ttcgagtctc tccgcccctg
     6721 ccattttttg ctaggtcggg caggaagagg gcctatttcc catgattcct tcatatttgc
     6781 atatacgata caaggctgtt agagagataa ttagaattaa tttgactgta aacacaaaga
     6841 tattagtaca aaatacgtga cgtagaaagt aataatttct tgggtagttt gcagttttaa
     6901 aattatgttt taaaatggac tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct
     6961 tggctttata tatcttgtgg aaaggacgaa acaccagggg cgtagttcaa ttggtagagc
     7021 accggtcttc aaaaccgggt gttgggagtt cgagtctctc cgcccctgcc attttttgct
     7081 aggtcgggca ggaagagggc ctatttccca tgattccttc atatttgcat atacgataca
     7141 aggctgttag agagataatt agaattaatt tgactgtaaa cacaaagata ttagtacaaa
     7201 atacgtgacg tagaaagtaa taatttcttg ggtagtttgc agttttaaaa ttatgtttta
     7261 aaatggacta tcatatgctt accgtaactt gaaagtattt cgatttcttg gctttatata
     7321 tcttgtggaa aggacgaaac accaggggcg tagttcaatt ggtagagcac cggtcttcaa
     7381 aaccgggtgt tgggagttcg agtctctccg cccctgccat tttttgctag tcaataatca
     7441 atgtcaacgc gtatatctgg cccgtacatc gcgaagcagc gcaaaacgga tcctgcaggc
     7501 tgatcactgc ttgagcctag ttattaatag taatcaatta cggggtcatt agttcatagc
     7561 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     7621 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     7681 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     7741 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
     7801 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
     7861 ttagtcatcg ctattaccat ggtcgaggtg agccccacgt tctgcttcac tctccccatc
     7921 tcccccccct ccccaccccc aattttgtat ttatttattt tttaattatt ttgtgcagcg
     7981 atgggggcgg gggggggggg ggggcgcgcg ccrggsgggg sggggsgggg sgrggggsgg
     8041 ggsggggsgr ggsggrgagg tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt
     8101 ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg
     8161 ggagtcgctg cgcgctgcct tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg
     8221 ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg cccttctcct
     8281 ccgggctgta attagcgctt ggtttaatga cggcttgttt cttttctgtg gctgcgtgaa
     8341 agccttgagg ggctccggga gggccctttg tgcgggggga gcggctcggg gggtgcgtgc
     8401 gtgtgtgtgt gcgtggggag cgccgcgtgc ggctccgcgc tgcccggcgg ctgtgagcgc
     8461 tgcgggcgcg gcgcggggct ttgtgcgctc cgcagtgtgc gcgaggggag cgcggccggg
     8521 ggcggtgccc cgcggtgcgg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg
     8581 tgcgtggggg ggtgagcagg gggtgtgggc gcgtcggtcg ggctgcaacc ccccctgcac
     8641 ccccctcccc gagttgctga gcacggcccg gcttcgggtg cggggctccg tacggggcgt
     8701 ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc gggcggggcg
     8761 gggccgcctc gggccggggr gggctcgggg gaggggcgcg gcggcccccg gagcgccggc
     8821 ggctgtcgag gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg
     8881 cagggacttc ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc
     8941 ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag
     9001 ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg gggctgtccg
     9061 cggggggacg gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg
     9121 accggcggct ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc
     9181 ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaatt ggccaaggag
     9241 gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     9301 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
     9361 acttgaggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     9421 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
     9481 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     9541 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     9601 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     9661 gggcacaagc tggagtacaa ctacaacagc cacaacgtct agatcatggc cgacaagcag
     9721 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
     9781 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     9841 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
     9901 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     9961 aaggggccct tcgaacaaaa actcatctca gaagaggatc tgaatatgca taccggtcat
    10021 catcaccatc accatcatca ccatcaccat cattaaggcc tctaaggccg aattcaacgc
    10081 gttaagtcga caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
    10141 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
    10201 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
    10261 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
    10321 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
    10381 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
    10441 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
    10501 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
    10561 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
    10621 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
    10681 gtcgacttta actcggccag cacagtggtc gatcgaccaa tgccctggct cacaaatacc
    10741 actgagatct ttttccctct gccaaaaatt atggggacat catgaagccc cttgagcatc
    10801 tgacttctgg ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt
    10861 gtctctcact cggaaggaca tatgggaggg caaatcattt aaaacatcag aatgagtatt
    10921 tggtttagag tttggcaaca tatgcccata tgctggctgc catgaacaaa ggttggctat
    10981 aaagaggtca tcagtatatg aaacagcccc ctgctgtcca ttccttattc catagaaaag
    11041 ccttgacttg aggttagatt ttttttatat tttgttttgt gttatttttt tctttaacat
    11101 ccctaaaatt ttccttacat gttttactag ccagattttt cctcctctcc tgactactcc
    11161 cagtcatagc tgtccctctt ctttgcggcc gcggtccgta tactccggaa tattaataga
    11221 tcatggagat aattaaaatg ataaccatct cgcaaataaa taagtatttt actgttttcg
    11281 taacagtttt gtaataaaaa aacctataaa tattccggat tattcatacc gtcccaccat
    11341 cgggcgcgaa ctcctaaaaa accgccacca tgaagtgcct tttgtactta gcctttttat
    11401 tcattggggt gaattgcaag ttcaccatag tttttccaca caaccaaaaa ggaaactgga
    11461 aaaatgttcc ttctaattac cattattgcc cgtcaagctc agatttaaat tggcataatg
    11521 acttaatagg cacagcctta caagtcaaaa tgcccaagag tcacaaggct attcaagcag
    11581 acggttggat gtgtcatgct tccaaatggg tcactacttg tgatttccgc tggtatggac
    11641 cgaagtatat aacacattcc atccgatcct tcactccatc tgtagaacaa tgcaaggaaa
    11701 gcattgaaca aacgaaacaa ggaacttggc tgaatccagg cttccctcct caaagttgtg
    11761 gatatgcaac tgtgacggat gccgaagcag tgattgtcca ggtgactcct caccatgtgc
    11821 tggttgatga atacacagga gaatgggttg attcacagtt catcaacgga aaatgcagca
    11881 attacatatg ccccactgtc cataactcta caacctggca ttctgactat aaggtcaaag
    11941 ggctatgtga ttctaacctc atttccatgg acatcacctt cttctcagag gacggagagc
    12001 tatcatccct gggaaaggag ggcacagggt tcagaagtaa ctactttgct tatgaaactg
    12061 gaggcaaggc ctgcaaaatg caatactgca agcattgggg agtcagactc ccatcaggtg
    12121 tctggttcga gatggctgat aaggatctct ttgctgcagc cagattccct gaatgcccag
    12181 aagggtcaag tatctctgct ccatctcaga cctcagtgga tgtaagtcta attcaggacg
    12241 ttgagaggat cttggattat tccctctgcc aagaaacctg gagcaaaatc agagcgggtc
    12301 ttccaatctc tccagtggat ctcagctatc ttgctcctaa aaacccagga accggtcctg
    12361 ctttcaccat aatcaatggt accctaaaat actttgagac cagatacatc agagtcgata
    12421 ttgctgctcc aatcctctca agaatggtcg gaatgatcag tggaactacc acagaaaggg
    12481 aactgtggga tgactgggca ccatatgaag acgtggaaat tggacccaat ggagttctga
    12541 ggaccagttc aggatataag tttcctttat acatgattgg acatggtatg ttggactccg
    12601 atcttcatct tagctcaaag gctcaggtgt tcgaacatcc tcacattcaa gacgctgctt
    12661 cgcaacttcc tgatgatgag agtttatttt ttggtgatac tgggctatcc aaaaatccaa
    12721 tcgagcttgt agaaggttgg ttcagtagtt ggaaaagctc tattgcctct tttttcttta
    12781 tcatagggtt aatcattgga ctattcttgg ttctccgagt tggtatccat ctttgcatta
    12841 aattaaagca caccaagaaa agacagattt atacagacat agagatgaac cgacttggaa
    12901 agtgataaaa gcttgtcgag aagtactaga ggatcataat cagccatacc acatttgtag
    12961 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
    13021 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
    13081 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
    13141 aactcatcaa tgtatcttat catgtctgga tctgatcact gcttgagcct aggagatccg
    13201 aaccagataa gtgaaatcta gttccaaact attttgtcat ttttaatttt cgtattagct
    13261 tacgacgcta cacccagttc ccatctattt tgtcactctt ccctaaataa tccttaaaaa
    13321 ctccatttcc acccctccca gttcccaact attttgtccg cccacagcgg ggcatttttc
    13381 ttcctgttat gtttttaatc aaacatcctg ccaactccat gtgacaaacc gtcatcttcg
    13441 gctacttt
//
