LOCUS       sequence-45562-e         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Seeliger JC, Topp S, Sogi KM, Previti ML, Gallivan JP, Bertozzi CR
  TITLE     A riboswitch-based inducible gene expression system for 
            mycobacteria.
  JOURNAL   PLoS One. 2012;7(1):e29266. Epub 2012 Jan 18.
  PUBMED    22279533
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(1)"; pages: "e29266. Epub 2012 Jan 18"
COMMENT     Sequence Label: pRiboS EcoHind
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     34..53
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     terminator      complement(203..249)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 tcgcggcctc gagcaagacg tttcccgttg aatatggctc ataacacccc ttgtattact
       61 gtttatgtaa gcagacagtt ttattgttca tgatgatata tttttatctt gtgcaatgta
      121 acatcagaga ttttgagaca caacgtggct ttcccccccc cccgacgtca ggtggctagc
      181 tgatcaccgc ggccatgatg gcataaaacg aaaggcccag tctttcgact gagcctttcg
      241 ttttatttga tgcctggcag tcgatcgtac gctagttaac tacgtcgaca tcgataagct
      301 tgaattctgc agctggatcc gcaattgtct tggccatctt gttgcctcct tagcagggtg
      361 ctgccaaggg catcaagacg atgctggtat caccggtacc tatagtgagt cgtatcccgg
      421 tcgcgagtgc caacgttatt cttagcactc gcctatgccg agtgcaagaa gccccgcacc
      481 gggtcatgcc cctcgttcga ccttgtcctc ggccctccga tccgggtgag tatgttcggc
      541 ccatgaccgc caacgacaac aagacccgta aatggtcggc cgcagacgtc cccgatcaaa
      601 gcgggcgcgt tgtggtcacc tctagattta aagatctggt accgcggccg cacgcgttgc
      661 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
      721 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
      781 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
      841 atcacaaaaa tcgacgctca agtcag
//
